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Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein

Nature volume 346, pages 623–628 (1990)Cite this article

Abstract

The three-dimensional structure of the amino-terminal 44K ATPase fragment of the 70K bovine heat-shock cognate protein has been solved to a resolution of 2.2 Å. The ATPase fragment has two structural lobes with a deep cleft between them; ATP binds at the base of the cleft. Surprisingly, the nucleotide-binding 'core' of the ATPase fragment has a tertiary structure similar to that of hexokinase, although the remainder of the structures of the two proteins are completely dissimilar, suggesting that both the phosphotransferase mechanism and the substrate-induced conformational change intrinsic to the hexokinases may be used by the 70K heat shock-related proteins.

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Authors and Affiliations

  1. Beckman Laboratories for Structural Biology, Department of Cell Biology, Stanford University School of Medicine, Stanford, California, 94305-5400, USA

    Kevin M. Flaherty, Camilla DeLuca-Flaherty & David B. McKay

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  1. Kevin M. Flaherty
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  2. Camilla DeLuca-Flaherty
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  3. David B. McKay
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Flaherty, K., DeLuca-Flaherty, C. & McKay, D. Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein. Nature 346, 623–628 (1990). https://doi.org/10.1038/346623a0

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