Abstract
SNARE proteins mediate membrane fusion. Neurosecretion depends on neuronal soluble NSF attachment protein receptors (SNAREs; SNAP-25, syntaxin-1, and synaptobrevin-1 or synaptobrevin-2) and is blocked by neurotoxin-mediated cleavage or genetic ablation. We found that exocytosis in mouse inner hair cells (IHCs) was insensitive to neurotoxins and genetic ablation of neuronal SNAREs. mRNA, but no synaptically localized protein, of neuronal SNAREs was present in IHCs. Thus, IHC exocytosis is unconventional and may operate independently of neuronal SNAREs.
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Acknowledgements
We thank M.C. Wilson for providing SNAP-25 knockout mice, A. Nystuen for providing lethal-wasting mice, D. Bruns and T.C. Südhof for providing synaptobrevin-2/3 double knockout mice, J. Sørensen for discussion, and A. Leonov and C. Griesinger for DM-Nitrophen. We would like to thank R. Jahn and E. Neher for comments on the manuscript and S. Blume, N. Dankenbrink-Werder and M. Köppler for technical assistance. This work was supported by grants of the Deutsche Forschungsgemeinschaft (800/1-1 to R.N. and MO896 2-1 to T.M.), the Max Planck Society (Tandem-Project to N.B. and T.M.) and by a fellowship of the Alexander von Humboldt Foundation (T.P.).
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The study was designed by R.N. and T.M. The experimental work was performed by R.N., J.N., A.V.B., E.R., T.P., T.F. and S.S. T.M., R.N., J.N., E.R., T.P., N.B. and T.B. prepared the manuscript.
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Nouvian, R., Neef, J., Bulankina, A. et al. Exocytosis at the hair cell ribbon synapse apparently operates without neuronal SNARE proteins. Nat Neurosci 14, 411â413 (2011). https://doi.org/10.1038/nn.2774
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DOI: https://doi.org/10.1038/nn.2774
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