Issue 24, 2011
From the journal:

Lab on a Chip

A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film†

Alexander D. Edwards,‡*ab   Nuno M. Reis,§‡a   Nigel K. H. Slatera  and  Malcolm R. Mackleya  

* Corresponding authors

a Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, UK
E-mail: a.d.edwards@reading.ac.uk
Fax: +44 (0) 118 378 4703
Tel: +44 (0) 118 378 4253

b Reading School of Pharmacy, Whiteknights, PO Box 224, Reading, UK

Abstract

We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200μm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.

Graphical abstract: A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film

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Article information

DOI
https://doi.org/10.1039/C0LC00357C
Article type
Technical Note
Submitted
31 Aug 2010
Accepted
04 Oct 2011
First published
27 Oct 2011

Lab Chip, 2011,11, 4267-4273

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A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film

A. D. Edwards, N. M. Reis, N. K. H. Slater and M. R. Mackley, Lab Chip, 2011, 11, 4267 DOI: 10.1039/C0LC00357C

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