Some studies show that a person’s characteristics, such as attractiveness or trustworthiness, could be perceived by looking at the eyes and the pupils [
16,
17]. In our study using only eyes with neutral expressions, we found large rating differences for exaggerated pupils (Experiment 1), but these differences were mostly absent for realistically looking pupils (Experiment 2). Pupils did not influence perceived sex and age, and they also did not systematically affect ratings of attractiveness, trustworthiness, intelligence, realism, familiarity, and valence. Contrary to the literature, we found no evidence of specific directional associations between pupils and ratings (i.e., big pupils denote higher ratings). Also, participants in both experiments could not perceive a person’s arousal from looking at the pupils. Therefore, it seems that the pupils, although when they are exaggerated could sometimes influence the perceived characteristics of another person, provide information mainly when interpreted in relation to other characteristics of facial expression.
4.1. How the Pupils Influence Perceived Characteristics of a Person
It seems unlikely that the pupils can influence the perceived sex and age of a person. In fact, we found no differences in sex and age ratings between the pupils. From the literature, biological sex influences eye size [
6,
37]. However, biological sex is unrelated to pupil size [
38,
39]. Age is typically perceived via features such as wrinkles [
9,
40]. However, pupil size is related to age. Older people have smaller pupils than younger people [
41]. This decrease in pupil size is also very small [
42] and difficult to perceive. We speculate that one could perceive age if the pupils appear cloudy (i.e., cataracts). This is due to the fact that cataracts are common in the older population [
43]. Nonetheless, it is unlikely that sex and age can be perceived from changing pupil sizes.
Other characteristics about a person may be perceived by looking at the pupils, which could be driven by more complex processes. Our data show that the perceived attractiveness, trustworthiness, intelligence, realism, familiarity, and valence, can be influenced by pupil size when looking at neutral eyes. However, our results do not show that pupil sizes alone influence how a person reads various characteristics about another person by looking at the eyes.
Kret and De Dreu [
17] showed that big (i.e., dilating) pupils appeared more attractive and trustworthy than small (i.e., constricting) pupils. In their experiment, big and small pupils differed by over 80% pupil area. If Kret and De Dreu’s results could be explained by pupil size differences, then we should also observe similar results between big and medium pupils in Experiment 1, since big and medium pupils differed by 100% in area. We found no differences between these two exaggerated pupils (Experiment 1). We also did not find any differences between big and small realistic pupils (Experiment 2) and they differed by twice the size (+100% area). Thus, it is unlikely that size alone can explain why the ratings differed from our study.
A key difference between our results and those from Kret and De Dreu’s study is motion. Although both studies used neutral eyes, our stimuli were static images, as compared to dynamic pupil changes. This hint that the changing of pupil sizes influences our perception regarding attractiveness and trustworthiness of another person. As a final remark, our results for exaggerated pupils changed in the same direction as those in Kret et al. [
17], despite being static images. Hence, it is possible that there may be an interaction between static pupil sizes and motion from dynamically changing pupils, which contribute to how we read different characteristics about a person by looking at their eyes.
4.2. Ecological Validity of Image Manipulation in Experiments
The directional associations between pupils and ratings shown in previous studies [
12,
17,
44] were missing from our study. Instead, in Experiment 1, eyes with small and vergence pupils were rated much lower than big and medium pupils in attractiveness, trustworthiness, intelligence, realism, familiarity, and valence. This finding was not replicated in Experiment 2 with more realistic pupils. This finding raises the question concerning to which extent ecological validity in stimulus preparation influences the generalizability of the results.
One explanation of the differences in our results might be related to the
uncanny valley effect. This effect refers to evoked feelings of uneasiness when an artificial agent or imagery looks almost human-like but not perfectly [
45]. It might be that in Experiment 1, the small pupils and the simulated vergence evoke
uncanny vibes, lessening the trustworthiness of the eyes in comparison to big pupils. Importantly, big pupils also looked unrealistic, but was not susceptible to the uncanny valley effect. Participants could have rated big pupils as less unrealistic, but not considered them uncanny based on a general understanding of pupillary response. Big pupils are uncommon in well-lit conditions for most every-day situations. The pupils constrict and become comparably small in response to light or changes in illumination [
46,
47]. Therefore, the exaggerated big pupils, although appearing unrealistic in Experiment 1, were not prone to the uncanny valley effect.
Laboratory experiments often take place in highly controlled environments so that manipulations can be accounted for. However, lab environments hardly mimic the real-world. When interacting in the real world, we often integrate multisensory cues to minimize communication errors [
48,
49]. These multisensorial cues are often missing in experiments to simplify the number of variables needed to be manipulated. As a result, realistic stimuli often produce smaller effects compared to artificial/schematic ones [
50]. In the context of our study, the eye-stimuli are also manipulated in unrealistic ways in existing literature, either by changing pupil sizes across an arbitrary duration [
16,
17], or by artificially modifying the sizes [
12]. However, these manipulations are not replicable in the real world.
Pupillary changes are dynamic and unpredictable in the real world. The pupils can change very quickly in response to minor fluctuations in luminance, cognitive processes, and arousal [
42,
51,
52]. Some studies suggest that our eyes can sense changes in another person’s pupil sizes through mimicking the pupil size of another person or animal during social interactions [
16,
53,
54]. However, these studies cannot reliably isolate the influence of luminance on pupil dynamics [
55,
56]. In fact, pupil dynamics may not be easily seen in the real world since most (79%) people have brown irises compared to blue (10%) and other colors across the world [
57]. There is also a spectrum of different shades for brown irises. Thus, it is difficult to say whether sensation of another person’s pupil size is possible despite the ever-changing luminance in the environment since there is a high chance that most people do not even see another person’s pupils due to the iris color.
It is unlikely that we can process changes in another person’s pupils unconsciously or consciously report the pupillary changes in real-world interactions. This is due to the fact that we do not make constant eye contact with whom we interact with. Eye contact can vary depending on the physical distance between people and the interaction’s phase. For example, eye contact is shorter and less frequent between the opposite sex, or when members are very close together [
58]. In contrast, eye contact is more common when someone is listening to a speaker [
59]. It is also unlikely that we pay close attention and look intently into another person’s eyes throughout the interaction as doing so causes the other person to feel uneasy. Since we do not make constant eye contact with others, it is less likely that we unconsciously process another person’s pupillary changes or become aware of such changes during real-world interactions. Hence, we can speculate that the impact of our measures may not replicate to real-world social interactions, since participants in our experiments were aware of the differently sized pupils and vergence when rating characteristics.
Lastly, an important aspect to consider is whether the stimulus preparation could be meaningful in drawing parallel conclusions about the real world. To that, some researchers have called for a clearer context-driven approach in defining ecological validity [
60], where research questions should be defined as context-specific questions. In any case, future experiments should adopt realistic and multisensory stimuli, whilst formulating specific contexts, to further investigate the pupils’ role in influencing how we perceive characteristics about another person by looking at their eyes.
4.3. Perceiving Arousal from the Pupils
Pupil dilation indicates arousal when pupil size changes are tracked in an observer [
61]. The pupils also dilate when we are emotionally stimulated [
62]. It seems that we may not be good at perceiving a person’s emotional arousal when looking at their pupils. This is since we found no differences in arousal ratings for both experiments. One criticism to our claim could be since the exaggerated pupils appeared absurd in Experiment 1. However, we did not find any differences when using more realistic pupils in Experiment 2. The findings may change if we had presented emotionally expressive eyes. However, this would suggest that the perceived emotional arousal was extracted by integrating information about the eye expression and the pupil size. In short, it is likely that we are bad at perceiving a person’s emotional arousal from looking at their pupils.
Additional visual signals besides the pupils are required to perceive a person’s arousal, specifically the sexual arousal. The pupils dilate when a person is sexually aroused. The pupils dilate in response to sexual attraction [
63]. Consequently, a person with larger pupils is perceived as more attractive [
44,
64]. One may even evaluate a stranger’s pupils in online dating to pick their potential partners [
65]. These studies show that pupil sizes influence perceived arousal (in terms of sexual arousal) when a full face is visible. This differs from our study where only the eyes were shown. Since we did not measure perceived sexual arousal, it remains possible that sexual arousal could be perceived by looking at the pupils in the eyes.
4.4. The Pupils as Important Cues in Interactions
It is important to investigate how the pupils influence what we perceive about another person. The pupils tell us where the eyes are gazing [
66,
67], so they help us understand how the eyes inform mutual interaction [
68]. In such interactions, pupil dilations communicate trust [
69], eye blinks signal the transition of conversational topics [
70], gaze patterns can indicate specific cognitive processes that are relevant for learning [
71] and recognizing eye movement patterns facilitates the recognition of facial expressions [
72]. Teachers who gaze into the students’ eyes during teaching are perceived to show greater interests in the student’s learning [
73]. Gazing at students also helps capture the students’ attention [
74] and fosters an interpersonal connection to their students, which is critical for the quality of teaching and learning [
75,
76]. With the recent surge in online-schooling, the frequency of making eye contact has fallen dramatically, creating severe problems in teaching as teachers must be able to observe their students’ attention to create an effective and efficient learning environment. In on-site teaching contexts, teachers can instantly pool information from many pupils to infer the direction of a group’s collective gaze [
77]. In online learning, this is not possible (since the webcam is not embedded in the screen), so alternative measures need to be considered in online education, such as mapping the teachers gaze point on the material for guiding the students [
78] or visualizing the aggregated gaze points of students [
79].
4.5. Limitations and Future Directions
The current study investigates the pupils in neutral static eye images. Thus, the results are specific only to static eyes without expressions. It is known that we rely on the eyes to identify fear and surprise [
80,
81]. Dynamic changes in the eye region also influence whether one correctly recognizes certain emotional expressions [
82]. Since the eyes also convey different emotions, it would be interesting to investigate how the interpretation of the current study changes, when eye emotions are introduced. Future studies could investigate the influence of varying pupils of either different static emotional eye expressions or dynamic eye expressions on the perceived characteristics of another person by looking at the eyes. The current sample sizes differ between the two experiments. Future experiments should aim for more balanced samples, especially when comparing data across experiments.
The addition of the first Purkinje image makes the eyes appear more lifelike. This is a technique commonly used in art. One criticism of Experiment 2 was that the reflections could have reduced the overall perceivable pupil size. According to the literature, the presence and absence of these reflections do not alter the perceived gaze of a painted portrait. It only affects the realism [
83]. Therefore, subsequent studies can compare whether the first Purkinje images reduce the overall perceived pupil size.
Cultural factors influence how often a person looks at the eyes. Eastern cultures have smaller emphasis on the eyes than the western culture [
84,
85]. We did not query participants’ ethnicity. Participant recruitment was also of convenient means (i.e., social media, friends, families). We also did not test eyes from other ethnicities. Hence, our conclusions are limited to the western culture.
It is without a doubt challenging in capturing the pupil dynamics in a controlled and reliable manner so that they mimic real-world behaviors. Nevertheless, future studies should strive for more ecological valid stimuli, such as recording real pupil dynamics, and presenting them as stimuli. Studies should also define context-specific problems to solve, so that ecological validity could be captured more precisely. Alternatively, the study could be conducted on real-person interactions where the experimental conditions elicit dilated or constricted pupils.