Markers of Epithelial–Mesenchymal Transition and Mucinous Histology Are Significant Predictors of Disease Severity and Tumor Characteristics in Early-Onset Colorectal Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Subjects
2.2. Immunohistochemical Analysis
2.3. Evaluation of E-Cadherin Expression
2.4. Evaluation of Vimentin Expression
2.5. Evaluation of Mucin-1 Expression
2.6. Pancytokeratin Staining and Tumor Budding Evaluation
2.7. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Non-Mucionous (n = 53) | Mucinous (n = 53) | p Value | |
---|---|---|---|
Age, years | 36.9 ± 5.2 | 35.7 ± 7.2 | 0.619 |
Male gender, % | 66.0 | 66.0 | 1.000 |
Tumor localization, % | |||
rectum + left colon | 81.1 | 60.4 | 0.039 * |
right colon | 18.9 | 39.6 | |
T stadium, % | |||
T2 | 13.2 | 11.3 | 0.587 |
T3 | 58.5 | 50.9 | |
T4 | 28.3 | 37.8 | |
N stadium, % | |||
N0 | 32.0 | 30.2 | 0.429 |
N1 | 30.2 | 41.5 | |
N2 | 37.8 | 28.3 | |
TNM stage, % | |||
I | 9.4 | 3.8 | 0.027 * |
IIA | 13.2 | 20.8 | |
IIB | 3.8 | 3.8 | |
IIC | 3.8 | 3.8 | |
IIIA | 3.8 | 0 | |
IIIB | 43.4 | 28.3 | |
IIIC | 16.9 | 11.3 | |
IVA | 3.8 | 9.4 | |
IVB | 1.9 | 0 | |
IVC | 0 | 18.8 | |
Tumor grade, % | |||
G1 + G2 | 94.3 | 73.6 | 0.007 * |
G3 | 5.7 | 26.4 | |
Tumor budding, % | |||
1 | 26.4 | 37.8 | 0.450 |
2 | 20.8 | 18.9 | |
3 | 52.8 | 43.3 | |
L positive, % | 73.1 | 84.8 | 0.218 |
V positive, % | 51.9 | 54.2 | 0.844 |
Mucin-1 score | 2.07 ± 0.96 | 3.15 ± 0.64 | <0.001 * |
E-cadherin score | |||
total | 1.84 ± 1.46 | 2.17 ± 1.09 | 0.112 |
membrane | 0.84 ± 0.96 | 0.95 ± 0.89 | 0.206 |
cytoplasmatic | 1.00 ± 0.62 | 1.21 ± 0.42 | 0.043 * |
Vimentin score | 0.85 ± 1.73 | 0.62 ± 1.47 | 0.473 |
Epithelial (n = 51) | Mesenchymal (n = 55) | p Value | |
---|---|---|---|
Age, years | 37.1 ± 7.2 | 35.6 ± 6.0 | 0.143 |
Male gender, % | 70.6 | 61.8 | 0.413 |
Tumor localization, % | |||
rectum + left colon | 72.5 | 65.5 | 0.726 |
right colon | 27.5 | 34.5 | |
T stadium, % | |||
T2 | 15.7 | 9.1 | 0.231 |
T3 | 58.8 | 50.9 | |
T4 | 25.5 | 40.0 | |
N stadium, % | |||
N0 | 39.2 | 23.6 | 0.091 |
N1 | 37.3 | 34.5 | |
N2 | 23.5 | 41.9 | |
TNM stage, % | |||
I | 9.8 | 3.6 | 0.198 |
IIA | 21.6 | 12.7 | |
IIB | 2.0 | 5.4 | |
IIC | 5.8 | 1.9 | |
IIIA | 2.0 | 1.9 | |
IIIB | 37.2 | 34.5 | |
IIIC | 7.8 | 20.0 | |
IVA | 2.0 | 10.9 | |
IVB | 0 | 1.9 | |
IVC | 11.8 | 7.2 | |
Tumor grade, % | |||
G1 + G2 | 92.2 | 76.4 | 0.034 * |
G3 | 7.8 | 23.6 | |
Tumor budding, % | |||
1 | 43.1 | 21.8 | <0.001 * |
2 | 31.4 | 9.1 | |
3 | 25.5 | 69.1 | |
L positive, % | 73.9 | 82.7 | 0.331 |
V positive, % | 44.7 | 60.4 | 0.160 |
Mucin-1 score | 2.56 ± 0.98 | 2.64 ± 0.98 | 0.716 |
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Djikic Rom, A.; Dragicevic, S.; Jankovic, R.; Radojevic Skodric, S.; Sabljak, P.; Markovic, V.; Stojkovic, J.R.; Barisic, G.; Nikolic, A. Markers of Epithelial–Mesenchymal Transition and Mucinous Histology Are Significant Predictors of Disease Severity and Tumor Characteristics in Early-Onset Colorectal Cancer. Diagnostics 2024, 14, 1512. https://doi.org/10.3390/diagnostics14141512
Djikic Rom A, Dragicevic S, Jankovic R, Radojevic Skodric S, Sabljak P, Markovic V, Stojkovic JR, Barisic G, Nikolic A. Markers of Epithelial–Mesenchymal Transition and Mucinous Histology Are Significant Predictors of Disease Severity and Tumor Characteristics in Early-Onset Colorectal Cancer. Diagnostics. 2024; 14(14):1512. https://doi.org/10.3390/diagnostics14141512
Chicago/Turabian StyleDjikic Rom, Aleksandra, Sandra Dragicevic, Radmila Jankovic, Sanja Radojevic Skodric, Predrag Sabljak, Velimir Markovic, Jovana Rosic Stojkovic, Goran Barisic, and Aleksandra Nikolic. 2024. "Markers of Epithelial–Mesenchymal Transition and Mucinous Histology Are Significant Predictors of Disease Severity and Tumor Characteristics in Early-Onset Colorectal Cancer" Diagnostics 14, no. 14: 1512. https://doi.org/10.3390/diagnostics14141512