Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Au-LFIA Test Strips
2.3. Pretreatment of Ground Pork Samples
2.4. Enrichment with Modified EC Broth and Evaluation by Using Au-LFIA Test Strip
2.5. Specificity of the Au-LFIA Test Strip
2.6. Identification of the False-Positive Bacterium
2.7. Cross Activity of C. freundii with Au-LFIA Strip
2.8. Optimization of Enrichment Conditions
2.9. Evaluation of the Optimized Enrichment by Using Au-LFIA Test Strip
3. Results and Discussion
3.1. Specificity of the Au-LFIA
3.2. False-Positive Result of Au-LFIA Test Strip
3.3. Isolation and Identification of C. freundii from Incubated Broth
3.4. Cross-Reactivity of C. freundii with Au-LFIA Strip
3.5. Optimization of the Modified Culture Medium for C. freundii Inhibition
3.6. Evaluation of Modified CT-SMAC in Ground Pork Test
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Species | Source | Result (Approx. 105 CFU/mL) |
---|---|---|
E. coli O157:H7 | ATCC 43888 | + |
E. coli O157:H7 | CMCC 44828 | + |
E. coli O157:H7 | NCTC 12900 | + |
E. coli O157:H7 | XY0540 a | + |
E. coli O157:H7 | XY0480 a | + |
Bacillus cereus | CMCC 63303 | − |
Bacillus cereus | CMCC 63305 | − |
Bacillus cereus | SLK a | − |
Bacillus licheniformis | CMCC 63519 | − |
Bacillus subtilis | BD366 a | − |
Candida albicans | ATCC 10231 | − |
Candida albicans | Z1 a | − |
Cronobacter cloacae | CMCC 45301 | − |
Cronobacter sakazakii | CMCC 45401 | − |
Cronobacter sakazakii | CMCC 45402 | − |
enteropathogenic E. coli | CMCC 44496 | − |
E. coli | CMCC 44102 | − |
E. coli | ATCC 25922 | − |
Lactobacillus bulgaricus | F1 a | − |
Listeria grayi | ATCC 25401 | − |
Listeria innocua | ATCC 33090 | − |
Listeria innocua | ATCC 11288 | − |
Listeria ivanovii | ATCC 19119 | − |
Listeria monocytogenes | ATCC 13932 | − |
Listeria monocytogenes | CMCC 54001 | − |
Listeria monocytogenes | CMCC 54007 | − |
Listeria welshimeri serovar 6b | ATCC 35897 | − |
Listeria seeligeri | ATCC 35967 | − |
Micrococcus luteus | CMCC 28001 | − |
Proteus vulgaris | CMCC 49027 | − |
Pseudomonas aeruginosa | ATCC 27853 | − |
Pseudomonas aeruginosa | CMCC 10104 | − |
Salmonella anatum | ATCC 9270 | − |
Salmonella choleraesuis | ATCC 13312 | − |
Salmonella choleraesuis | CICC 21493 | − |
Salmonella enteritidis | ATCC 13076 | − |
Salmonella paratyphi A | ATCC 9150 | − |
Salmonella enterica | ATCC 10708 | − |
Salmonella typhimurium | ATCC 13311 | − |
Serratia marcescens | CMCC 41002 | − |
Shigella flexneri | ATCC 12022 | − |
Shigella sonnei | CMCC 51592 | − |
Staphylococcus aureus | CMCC 26001 | − |
Staphylococcus aureus | CMCC 26003 | − |
Vibrio parahaemolyticus | CGMCC 1.1997 | − |
Samples | Negative Result of Au-LFIA | Positive Result of Au-LFIA | |
---|---|---|---|
Samples from the slaughterhouse | 10 positive samples | 0 | 20 |
10 negative samples | 0 | 20 | |
Samples from the supermarket | 10 positive samples | 0 | 20 |
10 negative samples | 12 | 8 |
Pork Samples | CHROmagarTM Plates | Au-LFIA Test Strip | ||
---|---|---|---|---|
+ | − | + | − | |
Positive samples (E. coli O157:H7) (n = 20) | 20 | 0 | 20 | 0 |
Negative samples (C. freundii) (n = 20) | 0 | 20 | 1 | 19 |
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Cheng, S.; Chen, M.-h.; Zhang, G.-g.; Yu, Z.-b.; Liu, D.-f.; Xiong, Y.-h.; Wei, H.; Lai, W.-h. Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay. Sensors 2017, 17, 753. https://doi.org/10.3390/s17040753
Cheng S, Chen M-h, Zhang G-g, Yu Z-b, Liu D-f, Xiong Y-h, Wei H, Lai W-h. Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay. Sensors. 2017; 17(4):753. https://doi.org/10.3390/s17040753
Chicago/Turabian StyleCheng, Song, Ming-hui Chen, Gang-gang Zhang, Zhi-biao Yu, Dao-feng Liu, Yong-hua Xiong, Hua Wei, and Wei-hua Lai. 2017. "Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay" Sensors 17, no. 4: 753. https://doi.org/10.3390/s17040753