Enhancement of Enzymatic Hydrolysis of Lignocellulosic Materials by Nonionic Surfactant

Jatuporn Parnthong; Suratsawadee Kungsanant; Sumaeth Chavadej

doi:10.4028/www.scientific.net/KEM.757.151

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 757Enhancement of Enzymatic Hydrolysis of...

Enhancement of Enzymatic Hydrolysis of Lignocellulosic Materials by Nonionic Surfactant

Abstract:

Palm fruit bunch (PFB) and palm fruit residue (PFR) are considered as potential raw materials for ethanol production due to their large availability from palm oil industry. However, the presence of lignin can retard the rate of enzymatic hydrolysis. Both PFB and PFR were pretreated with a 10% w/v sodium hydroxide solution. The amounts of their hemicellulose were substantially decreased whereas their lignin contents were slightly reduced. After that, they were hydrolyzed using cellulase from Trichoderma reesei (ATCC 26921) at 50 °C and pH 5. An addition of Tween 80, nonionic surfactant, with 0.25% w/v concentration provided an increase in reducing sugar production about 50.5% at 10 FPU/g PFB cellulase loading, while the addition of Tween 80 with 0.5% w/v concentration increased reducing sugar production by 38.8% at 20 FPU/g PFR cellulase loading. The greater the amount of lignin in the lignocellulosic materials, the higher the dosages of enzyme and surfactant required for the enzymatic hydrolysis. The adsorption of added nonionic surfactant onto the hydrophobic surface of lignin resulted in an increase in the availability of added enzyme to both cellulose and hemicellulose, leading to the enhancement of enzymatic hydrolysis. Additionally, the adsorption of cellulase on PFB and PFR were well fitted with the Freundlich isotherm.

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Periodical:

Key Engineering Materials (Volume 757)

Pages:

151-155

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.757.151

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Online since:

October 2017

Authors:

Jatuporn Parnthong, Suratsawadee Kungsanant*, Sumaeth Chavadej

Keywords:

Adsorption, Cellulase, Hydrolysis, Lignin, Nonionic Surfactant

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References

[1] P. Boonsawang, Y. Subkaree, T. Srinorakutara, Ethanol production from palm pressed fiber by prehydrolysis prior to simultaneous saccharification and fermentation (SSF), Biomass Bioenergy. 40 (2012) 127–132.

DOI: 10.1016/j.biombioe.2012.02.009

Google Scholar

[2] J. Parnthong, S. Kungsanant, Statistical optimization for application of nonionic surfactants in enzymatic hydrolysis of palm fiber for ethanol production, IJCE. 5 (2014) 23–25.

DOI: 10.7763/ijcea.2014.v5.344

Google Scholar

[3] Y. Li, Z. Sun, X. Ge, J. Zhang, Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose, Biotechnol. Biofuels. 9: 20 (2016) 1–9.

DOI: 10.1186/s13068-016-0434-0

Google Scholar

[4] T. Eriksson, J. Börjesson, F. Tjerneld, Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose, Enzyme Microb. Technol. 31 (2002) 353–364.

DOI: 10.1016/s0141-0229(02)00134-5

Google Scholar

[5] D.S. Hirotaka, F. Akiyoshi, Effects of a non-ionic surfactant, Tween 20, on adsorption/ desorption of saccharification enzymes onto/from lignocelluloses and saccharification rate, Adsorption. 17 (2011) 813–822.

DOI: 10.1007/s10450-011-9340-8

Google Scholar

[6] S. Palamae, W. Palachum, Y. Chisti, W. Choorit, Retention of hemicellulose during delignification of oil palm empty fruit bunch (EFB) fiber with peracetic acid and alkaline peroxide, Biomass Bioenergy. 66 (2014) 240–248.

DOI: 10.1016/j.biombioe.2014.03.045

Google Scholar

[7] G. L. Miller, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem. 31 (1959) 426–428.

DOI: 10.1021/ac60147a030

Google Scholar

[8] M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248–254.

DOI: 10.1016/0003-2697(76)90527-3

Google Scholar

[9] H.S. Shin, J. Kim, Isotherm, kinetic, and thermodynamic characteristics of adsorption of paclitaxel onto Diaion HP-20, Process Biochem. 51 (2016) 917–924.

DOI: 10.1016/j.procbio.2016.03.013

Google Scholar

[10] A. Adewuyi and F. Vargas, Nitrilotriacetic acid functionalized adansonia digitata biosorbent : Preparation, characterization and sorption of Pb (II) and Cu (II) pollutants from aqueous solution, JRA. 7 (2016) 947–959.

DOI: 10.1016/j.jare.2016.10.001

Google Scholar