doiSerbia

Olive leaf extract modulates cold restraint stress-induced oxidative changes in rat liver

Dekanski Dragana (Biomedical Research, R&D Institute, Galenika a.d., Belgrade)
Ristić Slavica (Biomedical Research, R&D Institute, Galenika a.d., Belgrade)
Radonjić Nevena V. (Institute for Medical and Clinical Biochemistry, School of Medicine, Belgrade)
Petronijević Nataša D. (Institute for Medical and Clinical Biochemistry, School of Medicine, Belgrade)
Dekanski Aleksandar (Institute for Chemistry, Technology and Metallurgy, Belgrade)
Mitrović Dušan M. (Institute for Medical Physiology “Richard Burian”, School of Medicine, Belgrade)

Recently, demonstrated the beneficial effects of different single doses of standardized dry olive (Olea europaea L.) leaf extract (OLE) in cold restraint stress (CRS)-induced gastric lesions in rats and its influence on oxidative parameters in gastric mucosa were demonstrated. The present study was undertaken to investigate the long-term pretreatment efficacy of OLE and its potential in the modulation of CRS-induced oxidative changes at the liver level. The experimental animals were divided into four groups, i.e., control, OLE-treated, CRS non-treated and CRS treated with OLE (CRS+OLE) groups. CRS caused severe gastric lesions in all non-pretreated animals and two-week pretreatment with OLE (80 mg kg-1 b.w.) attenuated stress-induced gastric lesions significantly. The malondialdehyde (MDA) level as an index of lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activities were measured spectrophotometrically in liver tissue homogenates. The MDA level was increased in the CRS group and significantly decreased in the CRS+OLE group. The SOD and CAT activities were significantly decreased in the CRS group. In the CRS+OLE group, the activities of these two enzymes were significantly increased in comparison with the CRS group. The results obtained indicate that long-term supplementation with OLE provides oxidant/antioxidant balance in liver during stress condition.

Keywords: olive leaf, cold restraint stress, oxidative stress, liver