B. licheniformis is an important host for the industrial production of enzymes mainly because of ... more B. licheniformis is an important host for the industrial production of enzymes mainly because of its ability to secrete large amounts of protein. We analyzed the proteome of Bacillus licheniformis cells growing in a minimal medium. Beside the cytosolic proteome, the membrane and the extracellular proteome were studied. We could identify 1470 proteins; 1168 proteins were classified as cytosolic proteins, 195 proteins with membrane spanning domains were classified as membrane proteins and 107 proteins, with either putative signals peptides or flagellin-like sequences were classified as secreted proteins. The identified proteins were grouped into functional categories and used to reconstruct cellular functions and metabolic pathways of growing B. licheniformis cells. The largest group were proteins with functions in basic metabolic pathways such as carbon metabolism, amino acid and nucleotide synthesis and synthesis of fatty acids and cofactors. Many proteins detected were involved in ...
According to Corey's retrosynthetic approach, 1 the chemical synthesis of individual target c... more According to Corey's retrosynthetic approach, 1 the chemical synthesis of individual target compounds requires the use of specific starting synthons in most cases. In contrast, the matrix approach 2 inspired by nature's biosynthetic machinery is based on a diversity-...
B. licheniformis is an important host for the industrial production of enzymes mainly because of ... more B. licheniformis is an important host for the industrial production of enzymes mainly because of its ability to secrete large amounts of protein. We analyzed the proteome of Bacillus licheniformis cells growing in a minimal medium. Beside the cytosolic proteome, the membrane and the extracellular proteome were studied. We could identify 1470 proteins; 1168 proteins were classified as cytosolic proteins, 195 proteins with membrane spanning domains were classified as membrane proteins and 107 proteins, with either putative signals peptides or flagellin-like sequences were classified as secreted proteins. The identified proteins were grouped into functional categories and used to reconstruct cellular functions and metabolic pathways of growing B. licheniformis cells. The largest group were proteins with functions in basic metabolic pathways such as carbon metabolism, amino acid and nucleotide synthesis and synthesis of fatty acids and cofactors. Many proteins detected were involved in ...
According to Corey's retrosynthetic approach, 1 the chemical synthesis of individual target c... more According to Corey's retrosynthetic approach, 1 the chemical synthesis of individual target compounds requires the use of specific starting synthons in most cases. In contrast, the matrix approach 2 inspired by nature's biosynthetic machinery is based on a diversity-...
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