Introduction: Natural compounds obtained from marine algae, especially Rhodophyta are now being i... more Introduction: Natural compounds obtained from marine algae, especially Rhodophyta are now being investigated for various biological activities. However, the antimicrobial activity and toxicity profile of compounds present in these algae isunderexplored. background: Bacterial resistance is becoming an imminent threat which necessitates the discovery of molecules capable of inhibiting their proliferation inside the host. Objective: This study procured two compounds, Debilon and Phorbasterone-B, from Rhodophyta to analyze their In-silico and in-vitro potential against pathogenic bacterial strains and their acute toxicity. objective: To exploit the physiochemical properties of Debilon and Phorbasterone-B for Antibacterial activity Methods: Debilon and Phorbasterone-B were extracted from Rhodophyta by a previously reported method and were further subsequently exploited computationally for their physicochemical properties, prediction of biological activity and molecular docking against ba...
Background and Objectives: The current study focuses on an eco-friendly and cost-effective method... more Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Results: Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical s...
The 3D polymer network of hydrogels got immense importance because of distinctive characteristics... more The 3D polymer network of hydrogels got immense importance because of distinctive characteristics like high water content, flexible and soft nature, biodegradable, and biocompatible behavior. These materials can be produced by physical or chemical cross-linking of the usual natural and synthetic hydrophilic polymer chains into a 3D network structure. The resemblances of hydrogels with the living body tissues make these materials to be applied extensively in the biomedical field. The present study emphasizes the main characteristics and use of hydrogels in biomedical fields. This review provides the reader with a comprehensive detail of the polymer hydrogel based inventions and products as well as perspective on future potential developments.
Preparation conditions for obtaining dense 8YSZ electrolyte films by dip-coating on NiO–YSZ anode... more Preparation conditions for obtaining dense 8YSZ electrolyte films by dip-coating on NiO–YSZ anode electrode substrates for SOFC application were investigated with focus on the co-sintering process. The NiO–YSZ substrates were prepared from nanoparticles which were obtained by a single modified sol–gel process using sucrose and pectin as organic precursors. The microstructure and the morphology of the product powders were analyzed by X-ray diffraction and TEM. A thin film of 8YSZ was obtained by a single-step dip-coating process using phosphate ester (PE) as dispersant and polyvinyl butyral (PVB) as binder. The microstructure of the sintered films was analyzed with SEM. The effect of the sinterability of the anode substrate on the 8YSZ film quality was studied. Fully dense electrolyte films were obtained on anode substrates which held a linear shrinkage of ∼23%. The use of substrates with a linear shrinkage below 23%, resulted in a poorly densified 8YSZ microstructure.
Background and Objectives: The current study focuses on an eco-friendly and cost-effective method... more Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Results: Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical scavenging and reducing power were 73.8 ± 0.32 µg/mg extract, 71.8 ± 0.73% and 105.4 ± 0.65 µg/mg extract respectively. The synthesized EpNPs were observed to possess high cytotoxicity against HepG2 cancer cell lines with IC50 (61.3 µg/mL) as compared aqueous extract with IC50 of (247 µg/mL). EpNPs were found to be biocompatible and have less effect on human erythrocytes. EpNPs exhibited significant antioxidant potentials and exhibited considerable activity against Escherichia coli and Bacillus subtilis with Minimum Inhibitory Concentration (MICs) of 11.12 µg/mL and 11.33 µg/mL respectively. Fungal species Aspergillus niger and Aspergillus flavus were found susceptible to EpNPs. Conclusions: Results of the current study revealed that EpNPs exhibited considerable antibacterial, antifungal and cytotoxic potentials. Aqueous extract possesses significant anti-radical properties and thus can be useful in free radicals induced degenerative disorders.
Stimuli-responsive biosensors are empirically measurable signatures for the detection of physiolo... more Stimuli-responsive biosensors are empirically measurable signatures for the detection of physiological status of environment and thus can act as clinical diagnosis devices. Poly(N-isopropylacrylamide) (PNIPAM) polymeric gels are widely explored as in vivo and in vitro thermoresponsive gels by changing their lower critical solution temperature (LCST). In this perspective, multi-responsive thin film sensors having polymer part composed of poly(N-isopropylacrylamide) (PNIPAM) integrated with photochromic dye (malachite green, MG) were prepared, which changed their behavior upon exposure to light and with the adjustment of the pH and temperature of their environment. The sensing materials were characterized by different techniques such as scanning electron microscopy (SEM), thermal analysis (TGA/DTA), UV–Vis spectroscopy, and reflectance spectroscopy to confirm their synthesis and integration of MG with PNIPAM. These sensors changed their color in the pH-responsive fields to yield patterns that change the reflectance spectra upon exposure to UV–visible light. By using thin films of low price, commonly available metals as coverslips, the reflectance response with light of these polymeric gels was measured. The response of these sensors was proved to be completely reversible and could be triggered multiple times. The Cr/Ag coverslips were found to be the best against environments with high or low pH values. In this investigation, we proved that a class of portable pH-sensing materials can be synthesized which can be implemented in specific drug delivery and display technologies in future.
Introduction: Natural compounds obtained from marine algae, especially Rhodophyta are now being i... more Introduction: Natural compounds obtained from marine algae, especially Rhodophyta are now being investigated for various biological activities. However, the antimicrobial activity and toxicity profile of compounds present in these algae isunderexplored. background: Bacterial resistance is becoming an imminent threat which necessitates the discovery of molecules capable of inhibiting their proliferation inside the host. Objective: This study procured two compounds, Debilon and Phorbasterone-B, from Rhodophyta to analyze their In-silico and in-vitro potential against pathogenic bacterial strains and their acute toxicity. objective: To exploit the physiochemical properties of Debilon and Phorbasterone-B for Antibacterial activity Methods: Debilon and Phorbasterone-B were extracted from Rhodophyta by a previously reported method and were further subsequently exploited computationally for their physicochemical properties, prediction of biological activity and molecular docking against ba...
Background and Objectives: The current study focuses on an eco-friendly and cost-effective method... more Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Results: Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical s...
The 3D polymer network of hydrogels got immense importance because of distinctive characteristics... more The 3D polymer network of hydrogels got immense importance because of distinctive characteristics like high water content, flexible and soft nature, biodegradable, and biocompatible behavior. These materials can be produced by physical or chemical cross-linking of the usual natural and synthetic hydrophilic polymer chains into a 3D network structure. The resemblances of hydrogels with the living body tissues make these materials to be applied extensively in the biomedical field. The present study emphasizes the main characteristics and use of hydrogels in biomedical fields. This review provides the reader with a comprehensive detail of the polymer hydrogel based inventions and products as well as perspective on future potential developments.
Preparation conditions for obtaining dense 8YSZ electrolyte films by dip-coating on NiO–YSZ anode... more Preparation conditions for obtaining dense 8YSZ electrolyte films by dip-coating on NiO–YSZ anode electrode substrates for SOFC application were investigated with focus on the co-sintering process. The NiO–YSZ substrates were prepared from nanoparticles which were obtained by a single modified sol–gel process using sucrose and pectin as organic precursors. The microstructure and the morphology of the product powders were analyzed by X-ray diffraction and TEM. A thin film of 8YSZ was obtained by a single-step dip-coating process using phosphate ester (PE) as dispersant and polyvinyl butyral (PVB) as binder. The microstructure of the sintered films was analyzed with SEM. The effect of the sinterability of the anode substrate on the 8YSZ film quality was studied. Fully dense electrolyte films were obtained on anode substrates which held a linear shrinkage of ∼23%. The use of substrates with a linear shrinkage below 23%, resulted in a poorly densified 8YSZ microstructure.
Background and Objectives: The current study focuses on an eco-friendly and cost-effective method... more Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Results: Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical scavenging and reducing power were 73.8 ± 0.32 µg/mg extract, 71.8 ± 0.73% and 105.4 ± 0.65 µg/mg extract respectively. The synthesized EpNPs were observed to possess high cytotoxicity against HepG2 cancer cell lines with IC50 (61.3 µg/mL) as compared aqueous extract with IC50 of (247 µg/mL). EpNPs were found to be biocompatible and have less effect on human erythrocytes. EpNPs exhibited significant antioxidant potentials and exhibited considerable activity against Escherichia coli and Bacillus subtilis with Minimum Inhibitory Concentration (MICs) of 11.12 µg/mL and 11.33 µg/mL respectively. Fungal species Aspergillus niger and Aspergillus flavus were found susceptible to EpNPs. Conclusions: Results of the current study revealed that EpNPs exhibited considerable antibacterial, antifungal and cytotoxic potentials. Aqueous extract possesses significant anti-radical properties and thus can be useful in free radicals induced degenerative disorders.
Stimuli-responsive biosensors are empirically measurable signatures for the detection of physiolo... more Stimuli-responsive biosensors are empirically measurable signatures for the detection of physiological status of environment and thus can act as clinical diagnosis devices. Poly(N-isopropylacrylamide) (PNIPAM) polymeric gels are widely explored as in vivo and in vitro thermoresponsive gels by changing their lower critical solution temperature (LCST). In this perspective, multi-responsive thin film sensors having polymer part composed of poly(N-isopropylacrylamide) (PNIPAM) integrated with photochromic dye (malachite green, MG) were prepared, which changed their behavior upon exposure to light and with the adjustment of the pH and temperature of their environment. The sensing materials were characterized by different techniques such as scanning electron microscopy (SEM), thermal analysis (TGA/DTA), UV–Vis spectroscopy, and reflectance spectroscopy to confirm their synthesis and integration of MG with PNIPAM. These sensors changed their color in the pH-responsive fields to yield patterns that change the reflectance spectra upon exposure to UV–visible light. By using thin films of low price, commonly available metals as coverslips, the reflectance response with light of these polymeric gels was measured. The response of these sensors was proved to be completely reversible and could be triggered multiple times. The Cr/Ag coverslips were found to be the best against environments with high or low pH values. In this investigation, we proved that a class of portable pH-sensing materials can be synthesized which can be implemented in specific drug delivery and display technologies in future.
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Papers by Mehwish Shah