haider faisal

Background: Hodgkin's lymphoma (HL) and Non-Hodgkin's lymphoma(NHL) are characterized by a profound disturbance of the cell cycle and apoptosis regulation., Ki-67 and P53 expression has been shown to be associated with adverse clinical outcome in a variety of malignant hematological disorders, including HL and NHL. Aim: This study aimed to clarify Ki-67 expression level and its correlation with p53 expression in HL and NHL. Material and methods: total of 28 newly diagnosed HL and NHL patients were investigated for Ki-67 expression and its correlation to p53 expression by immunohistochemical technique. Results: Ki-67 expression was observed in 46.7 % of HL patients while 7.7% of NHL patients gave positive Ki-67 expression. A significant Association between thetype of lymphoma and Ki67 expression (p<0.05) noticed. The histopathologcal type appeared to be 57.1% mixed cellularity and 37.5% of them showed positive Ki67 expression , while 42.9 % were intermediate grade and only 16.7 % showed positive Ki67 expression. There were no significant correlation between the histopathologcal type and Ki67 expression. only 4 (14.3 %) of the cases were p53 negative ,while the rest 24 cases were p53 positive ,and the majority (66.7%) were within score 2. Eight cases (28.6 %) were positive for Ki-67 and p53 , Ki-67 was negative in 71.4% cases , and four of them were negative for p53 and the rest 16 (80%)cases were p53 positive .A significant correlation was found between p53 and Ki-67 expression where(r=0.608,p=0.001). conclusion : it is suggested that immunohistochemical studies of p53 and Ki-67 expression in tumor tissue including HL and NHL can help in monitoring of patients at risk, and the markers may aid in controlling the progression of lymphoma and detect the degree of aggressiveness of the disease to give suitable treatment and management of patients .

Background: Recurrent pregnancy loss (RPL) has been found to be associated with increase in the pro-inflammatory cytokines which cause up-regulation of inflammatory mediators including cell adhesion molecules (CAMs) that might act in aggravation of this pathological process. Objective: To find out whether there is a relation between the pathology of RPL and the expression of intracellular adhesion molecule-1 (ICAM-1) and ICAM-3 at the feto-maternal interface in these patients. Methods: Immunohistochemistry technique was performed to detect and determine the expression of ICAM-1 and ICAM-3 using paraffin embedded sections of curate samples obtained from 40 women, who where divided into three groups: 24 women with RPL, 10 women with abortion for the first time, and 6 women with induced abortion.

Background: There are several regulatory proteins involved in the control of lymphocyte apoptosis. Their impairment may play a role in the pathogenesis of several autoimmune diseases. Recent studies have reported impairment in the apoptosis of peripheral blood lymphocytes (PBLs) in patients with rheumatoid arthritis (RA). Objective: The aim of this study is to evaluate the cellular expression of P53 and BcI-2 proteins in the PBLs and their roles in the apoptotic process, and correlate their cellular expressions with the percent of peripheral T cell population. Methods: This study involved forty-six RA patients were examined and compared with 17 healthy control individuals of similar ages. Lymphocytes were separated from peripheral blood samples, the assessment of their cellular expression of CD3 and regulatory proteins p53 and Bcl-2 by immunocytochemistry staining method. Results: The results showed abundant accumulation of CD3 T lymphocytes in the peripheral circulation of RA patients in comparison with controls. A highly significant increased percentage of Bcl-2

In Crohn’s disease, the interaction between ATG16L1 genetic variant and Murine Norovirus infection has been proposed. This study aims to estimate the association of Human Norovirus infection with ATG16L1 genetic variant among inflammatory bowel disease patients. This Case- control study involves 35 Crohn’s disease (CD) and 40 ulcerative colitis (UC) and 35 normal subjects obtained from 3 gastroenterology centers in Baghdad. Sequence specific primer polymerase chain reaction (SSP- PCR) used for ATG16L1 genotyping. Biopsies were sectioned and Norovirus by indirect immunofluorescence staining. Among Iraqis, T300A genetic variant confirmed as a risk factor in CD than HC (OR=2.57) or UC (OR=2.76). Among IBD patients, 9 (25.7%) CD and 7 (17.5%) UC patients were infected with Norovirus. 6 (50%) of homozygous mutant (GG) have Norovirus and 2 (20%) of heterozygous (GA) while, homozygous wild type have no evidence of Norovirus in their tissue biopsies. Among G allele carriage 38.5% were positive while 9.68% among allele A carriage were Norovirus positive. Among clinical samples the interaction between host genetics and viral infection.

Human infection with Brucella spp. had been able to evoke humeral immune response containing both IgG and IgM.
This study designed to compare results obtained from Rose Bengal Test (RBT), Tube Agglutination Test (TAT) and Enzyme Linked Immunosorbent Assay (ELISA) employ serum are described and compared for the detection of human IgG and IgM anti-brucella antibodies.
Serum samples from 105 subjects were collected. 90 were clinically infected with human brucellosis,
and 15 were age and gender matched controls. RBT and TAT are the two screening tests routinely recognized, while the 2-mercaptoethanol test (2ME) is the confirmatory assays currently in use. In order to improve the serological diagnosis of human brucellosis, an indirect IgG, IgM and IgG-IgM ELISA kits were evaluated
Totally, 90 cases were positive in RBT, from those only 92% shows positive TAT, and by ELISA there are IgG (27.78%), IgM (14.44%) and (57.78%) were Positive for both immunoglobulins.
Although RBT and TAT are widely applied tests, they cannot differentiate acute and chronic states of brucellosis. Our data suggest that IgM ELISA may be a suitable test for diagnosis acute brucellosis.

Glioma is one of the most aggressive and most common tumors of the central nervous system (CNS) in humans. The exact causes of glioma are not well known, but evidence suggests the involvement of genetic factors in addition to environmental risk factors. The present study aimed to determine whether polymorphisms in IL-10-1082A/G, IL-12p40 1188C/A, and IL-13+2044G/A (rs20541) are associated with the incidence of glioma in Iraqi patients. Ninety-six patients with different grades of glioma and 40 apparently healthy individuals were recruited. A blood sample and genomic DNA were collected from all subjects. The amplification refractory mutation system and sequence-specific primer polymerase chain reaction (PCR) were used for genotyping of IL-10-1082A/G and IL-12p40 1188C/A, respectively; whereas, the IL-13+2044G/A was detected by DNA sequencing after amplification of the genes by PCR.

Autophagy related 16 like 1 gene (ATG16L1) Thr300Ala genetic variant confer an increased risk for the development of Crohn s disease. According to Montreal disease classification, this study aims to determine the clinical significance of ATG16L1 T300A genetic variant among those patients. This case control study
involved 35 CD, 40 UC and 35 HC. After extraction of DNA from blood samples, ATG16L1 T300A genotyping were done by SSP-PCR. Immunohistological techniques done for localization of ATG16L1, LC3C, Lysozyme, IL-17A, Norovirus and H. pylori in tissue samples of all subjects. In this study, we observed an association of ATG16L1 Thr300Ala genetic variants with CD (55.71%) conferring higher risk for the disease development (OR=2.76, 95% CI= 1.3-5.1), rather than UC, the genetic variation was 31.25% showed no association with disease development (OR=0. 93, 95% CI= 0.4-1.8) when compared with HC 32.8%. Clinically, patients with CD risk allele had non-specific clinical phenotype, but they tend to have an extra-intestinal manifestations (75%) and the need for surgical intervention (76.4%). This study concluded that ATG16L1 T300A genetic
variant (CD risk allele) is a risk factor for CD development rather than UC.