Phaedra (Faidra) Eleftheriou

International Hellenic University - IHU, Biomedical sciences, Faculty Member

ATEI Thessalonikis, Medical Laboratoy Study, Faculty Member

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Eleftheriou Phaedra (Faidra)Education: Bachelor of Chemistry, AUTH, PhD in Biochemistry, AUTH.Present position: Professor of Clinical Chemistry – Biochemistry, Department of Biomedical Sciences, School of Health, International Hellenic University. Research Interests: ∎Study of Immunologic response to food derived antigens and its relation to pathologic disorders including several cancer types, autoimmune and metabolic disorders. Computer-aided investigation of the related mechanism based on protein structure relationship and protein-protein interactions. Investigation of their use as disease prognosis or monitoring tool.∎Effect of the Diet, Life Style and Environmental Factors at the biological markers of healthy individuals.∎Correlation of biochemical markers with disease pathophysiology ∎Molecular modeling applications in the investigation of Disease pathophysiology and Therapy. ∎ Computer-Aided Rational design of specific enzyme inhibitors with application in Diagnosis and Therapy, biological activity testing of new compounds and natural products as enzyme inhibitors with therapeutic and diagnostic interest. ∎ Enzyme Inhibitors with Applications in Diagnosis and Therapy: Phosphatase Inhibitors: SHP-2 (JMML), PTP1B, LAR (Diabetes II), etc. Squalene synthase inhibitors: SQS (dyslipidemia) inhibitors of COX-1/2, LOX (anti-inflammatory agents), HIV-1 reverse transcriptase and integrase inhibitors, etc. Teaching in Undergraduate courses: Biochemistry (ATEITH, IHU), Clinical Chemistry (ATEITH, IHU), Biochemical Pharmacology – Toxicology (IHU), Enzymology-Enzyme Engineering, Cell Culture and Laboratory Animals, Molecular Diagnostics, Medical Biotechnology (ATEITH), Enzymology (Department of Biochemistry and Biotechnology, University of Thessaly). Laboratory exercise in Pharmacology and Medicinal Chemistry (AUTH) Teaching of Post-Graduate Courses: -2013 – 2018: Advanced Glycation End Products, Master in “Diabetes Mellitus Care” A.TEI.TH, Greece.-2014-2020: Enzymes

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Papers by Phaedra (Faidra) Eleftheriou

In Silico Evaluation of the Effectivity of Approved Protease Inhibitors against the Main Protease of the Novel SARS-CoV-2 Virus

by Phaedra (Faidra) Eleftheriou and Dionysia Amanatidou

article, 2020

The coronavirus disease, COVID-19, caused by the novel coronavirus SARS-CoV-2, which first emerge... more The coronavirus disease, COVID-19, caused by the novel coronavirus SARS-CoV-2, which first emerged in Wuhan, China and was made known to the World in December 2019 turned into a pandemic causing more than 126,124 deaths worldwide up to April 16th, 2020. It has 79.5% sequence identity with SARS-CoV-1 and the same strategy for host cell invasion through the ACE-2 surface protein. Since the development of novel drugs is a long-lasting process, researchers look for effective substances among drugs already approved or developed for other purposes. The 3D structure of the SARS-CoV-2 main protease was compared with the 3D structures of seven proteases, which are drug targets, and docking analysis to the SARS-CoV-2 protease structure of thirty four approved and on-trial protease inhibitors was performed. Increased 3D structural similarity between the SARS-CoV-2 main protease, the HCV protease and α-thrombin was found. According to docking analysis the most promising results were found for HCV protease, DPP-4, α-thrombin and coagulation Factor Xa known inhibitors, with several of them exhibiting estimated free binding energy lower than −8.00 kcal/mol and better prediction results than reference compounds. Since some of the compounds are well-tolerated drugs, the promising in silico results may warrant further evaluation for viral anticipation. DPP-4 inhibitors with anti-viral action may be more useful for infected patients with diabetes, while anti-coagulant treatment is proposed in severe SARS-CoV-2 induced pneumonia.

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Terpenes with HIV-1 reverse transcriptase inhibitory action

Novel 2-(chloro/fluorophenyl)-3-(chloro/fluorobenzo[d]thiazol-2-yl) thiazolidin-4-one derivatives as HIV-1 Reverse Transcreptase inhibitors

Novel 2-(adamantan-1-yl)-5-[(aryl)methylidene]-[1,2,4] triazolo[3,2-b][1,3]thiazol-6-one derivatives as potential anti-inflammatory drugs with COX1/COX2

Prevalence of anti-Neu5Gc antibodies in a sample of general population and in patients with thyroid glad diseases and rheumatoid arthritis

Novel 3,4-diphenyl-1,2,4-triazole Derivatives as Potential Anti-inflammatory Drugs with COX1/COX2 Inhibitory Action

ABSTRACT

Evaluation of HIV-1 Reverse Transcriptase Inhibitory Action of Different Thiazolidinone Derivatives

ABSTRACT

Novel thiazole/benzothiazol/benzoisothiazolyl-5-aryliden-4-thiazolidinones with COX/LOX inhibitory activity

Serum antioxidant status among young, middle-aged and elderly people before and after antioxidant rich diet

Hippokratia

The influence of factors such as age, sex, life style and smoking on oxidative stress status of t... more The influence of factors such as age, sex, life style and smoking on oxidative stress status of the organism remains unclear. There is evidence that dietary intake of antioxidants is thought to enforce the organism ability to counteract free radicals. Administration of synthetic antioxidants as dietary supplements does not seem to have the same beneficial effect as consumption of the same antioxidants as part of food ingredients. This work focuses on the investigation of age and diet effects on oxidative stress and examines the hypotheses of their significant influence. Blood samples of 146 volunteers, were collected and allocated in three age groups. All volunteers completed a questionnaire concerning home and working environmental conditions, special habits and dietary preferences. We implemented a thirty days diet rich in antioxidants in 55 volunteers. Antioxidant activity was estimated before and after the special diet by measuring the influence of serum in oxidation of ABTS by the ferryl myoglobinhydrogen peroxide system. Our findings showed unexpected lower serum total antioxidant capacity (TAC) in younger people (ages 18-35 yrs) 79%, compared to middle aged and elderly individuals and a large increase 62% in serum TAC of all age-groups after the one-month special diet. These results imply that a diet rich in antioxidants based on antioxidant rich food consumption and not on single antioxidants administration, can increase the antioxidant status of the organism and offer better health. The total serum antioxidant status increases with age and this fact should be taken into account when TAC is measured in different diseases.