The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (C... more The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (Cd) induced chromosomal aberrations of bone marrow and sperm abnormalities. Forty five (45) adult male albino mice were caged into six groups. Mice were injected, i.p, 300 mg HY/kg and/or 0.67 mg CdCl 2 /kg b.w for 96 h, separately and alternated. The alternated trials were continued for consecutive eight days. Results show that mice injected with cadmium had significant increase in the frequency of aberrant chromosomes as fragment, centric fusion, gap, stickiness and aneuploidy and in sperm abnormality. The administration of HY improved the frequency of the chromosomal aberrations and sperm abnormality induced by Cd.
The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hem... more The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hematological parameters of the Nile tilapia (Oreochromis niloticus ) were studied. The 96 hrs LC50 of profenofos to Nile tilapia was determined to be 0.87 mg/l. No detectable profenofos in aquaria water was observed after 14, 21 and 28 days of trial period and during recovery. Profenofos were accumulated in higher rate fish tissues during the acute exposure. However, during chronic period a gradual increase was observed through 1 to 7 days followed by gradual decrease until it was not detectable at the end of 28 days. Regarding hematology, highly significant increase in WBCs counts during both the acute and chronic exposure to was observed. Highly significant decrease in RBCs counts, Hb content and Hct % was noticed during experimental periods. For most of the recorded data, it showed marked improvement during the recovery period. There was a significant increase in MCHC during both acute and chronic periods which were restored to its normal values by the end of chronic period. During the acute intoxication, MCH and MCV values showed highly significant decrease. Thereafter, MCH and MCV restored its normal level, except MCV on day 7th during the chronic exposure. In conclusion, profenofos exert an alteration of fish hematological parameters and profenofos deposited in their flesh.
The induction of 70 kDa heat - shock protein (hsp70) in the Nile tilapia, Oreochromis niloticus, ... more The induction of 70 kDa heat - shock protein (hsp70) in the Nile tilapia, Oreochromis niloticus, was investigated. The accumulation of hsp70 was determined in tissues of the liver, gills, spleen and heart of heat shocked fish. Other specimens were also exposed for 96 hr to four concentrations (1,0, 0.5, 0.1 and 0.001 mg/1) of Cu as copper sulphate and the accumulation of hsp70 was determined in the liver and gills. In both experiments, antibodies raised against hsp70 were employed as probes for dot-blot analysis and immunobinding assay of tilapia tissues. Decreasingly, hsp70 was abundant in the spleen, gills, heart and iiver of heat shocked fish. Also, data showed greater hsp70 level in the liver and gills of fish exposed to elevated Cu concentrations than the that of controls. These data suggest that hsp70 accumulation may hold a promise as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.
Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different ... more Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different localities in Qalyobya governorate, i.e., unpolluted, agricultural-polluted and industrial-polluted waters of River Nile. Water analysis was carried out to evaluate the heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution. The current study aims to revealing the effect of the water pollution, both agricultural and industerial, on the proteinogram and DNA damage in fish inhabiting these polluted areas of River Nile. The study suggested that there is clear molecular changes in T. zillii; this declares the use of these molecular parameters in diagnosing pollution.
Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egyp... more Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egypt, may find its way to water system and adversely effect on aquatic life particularly fish. Nile tilapia, Oreochromis niloticus as a major fish species in River Nile and one of the major sources of protein for human beings in Egypt, and it can also be a source of threaten to human health. Transport profenofos directly to tilapia fish may affect their physiological status and then fish production. The mortality of profenofos toxicity was estimated on tilapia and LC50 was detected as 0.87 mg/l. Also fish were exposed to 1/2 LC50 for 96 hrs and to 1/10 LC50 for 28 days and lastly were left after the chronic toxicity for another 28 days as recovery period. The increase of blood glucose was accompanied with decrease in liver and muscles glycogen throughout the acute and chronic trail periods. Fish showed also highly significant decrease in serum total protein and globulin with increasing in albumin and A/G ratio. A sharp elevation in serum creatinine, urea and uric acid with decrease in serum total lipid, triglyceride and cholesterol were also recorded. Lastly gradual and sharp elevation in the levels of serum enzymes, S-AST, S-ALT and S-ALP was revealed in profenofos-exposed tilapia. Same behavior as S-AST and S-ALT were in liver transaminases (L-AST & L-ALT). Our study revealed adverse change of metabolism in tilapia due to profenofos exposure. This may inform about the dangerous use of profenofos and limitations should be managed.
Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. I... more Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. It receives sewage water from Kafr Shanawan drainage canal. Also, it receive illegal run–off from sewage and wastes besides sewage of Shebeen Al-koom city. This study is aimed to investigate the frequency of chromosomal damage by using chromosomal aberrations test of head kidney cells in Oreochromis niloticus and Tilapia zillii fish. The study was performed on 5 groups of fish, control and four polluted groups. A and B stations are located before the source of sewage discharge while C and D stations are located after the source of sewage. In each group cytological studies were performed. Fishes caught from areas before the sources of sewage showing different types of chromosomal aberrations, including centromeric attenuations, chromatid breaks, chromatid gabs, chromatid deletion, centric fusion and fragmentation. The total aberrations were more obvious among the fishes of the areas after the source of sewage than those of the areas before the source of sewage. It was noted that O. niloticus is more sensitive to the effect of pollution than T. zillii. Cytogenetic Studies of the current Work could be used as criteria for pollution intensity, which can be used to avoid its toxic effect on aquatic environment.
The present study was carried out to investigate the effect of sublethal zinc (Zn)
concentrations... more The present study was carried out to investigate the effect of sublethal zinc (Zn) concentrations on the growth, biochemical variables, and Zn residues in common carp, Cyprinus carpio L.. Fish (18.1 – 19.1 g) were exposed to 0.0 (control), 5.0 and 10.0 mg Zn/L for 7, 14, 28, and 56 days. At each time interval and each treatment, fish were collected, weighed and sampled to measure the growth, biochemical variables, and Zn residues in whole-fish body. Growth performance was significantly reduced with increasing Zn concentrations. However, fish exposed to 10.0 mg Zn/L for 56 days grew lower than that of the control group. Likewise, the optimum feed intake and feed conversion ratio were obtained at control group at 56 days. Furthermore, glucose, AST, ALT, creatinine, and cortisol increased significantly with increasing Zn concentration and exposure time, with maximal values at 56 days. Meanwhile, the highest values of serum protein and lipids of were obtained in the control fish reared for 56 days, whereas the lowest values were observed in fish exposed to 10.0 mg Zn/L for 56 days. The content of whole-body moisture and total ash increased significantly, while crude protein and total lipid contents decreased significantly with increasing Zn concentrations. In addition, Zn exposure increased Zn residues in fish body; however, Zn bioaccumulation in fish body was Zn dose and time dependant. The present study revealed that the growth and health status of common carp were deteriorated by Zn toxicity.
The present study deals with the effect of some water pollutants; copper sulfate as a fungicide, ... more The present study deals with the effect of some water pollutants; copper sulfate as a fungicide, malathion as an insecticide and paraquat as a herbicide, on ;:'_rum total protein serum albumin and globulin. Furthermore, proteinogram of blood serum niloticus in Oreochromis was done using electrophoresis. It was found that the control fish have nine serum protein fractions. The exposure of fish to the examined pollutants induced a disappearance of some fractions and consequently changes of relative mobility and area that indicate genetic damage.
The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (C... more The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (Cd) induced chromosomal aberrations of bone marrow and sperm abnormalities. Forty five (45) adult male albino mice were caged into six groups. Mice were injected, i.p, 300 mg HY/kg and/or 0.67 mg CdCl 2 /kg b.w for 96 h, separately and alternated. The alternated trials were continued for consecutive eight days. Results show that mice injected with cadmium had significant increase in the frequency of aberrant chromosomes as fragment, centric fusion, gap, stickiness and aneuploidy and in sperm abnormality. The administration of HY improved the frequency of the chromosomal aberrations and sperm abnormality induced by Cd.
The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hem... more The effect of acute and chronic exposure of pesticide profenofos on its accumulation and some hematological parameters of the Nile tilapia (Oreochromis niloticus ) were studied. The 96 hrs LC50 of profenofos to Nile tilapia was determined to be 0.87 mg/l. No detectable profenofos in aquaria water was observed after 14, 21 and 28 days of trial period and during recovery. Profenofos were accumulated in higher rate fish tissues during the acute exposure. However, during chronic period a gradual increase was observed through 1 to 7 days followed by gradual decrease until it was not detectable at the end of 28 days. Regarding hematology, highly significant increase in WBCs counts during both the acute and chronic exposure to was observed. Highly significant decrease in RBCs counts, Hb content and Hct % was noticed during experimental periods. For most of the recorded data, it showed marked improvement during the recovery period. There was a significant increase in MCHC during both acute and chronic periods which were restored to its normal values by the end of chronic period. During the acute intoxication, MCH and MCV values showed highly significant decrease. Thereafter, MCH and MCV restored its normal level, except MCV on day 7th during the chronic exposure. In conclusion, profenofos exert an alteration of fish hematological parameters and profenofos deposited in their flesh.
The induction of 70 kDa heat - shock protein (hsp70) in the Nile tilapia, Oreochromis niloticus, ... more The induction of 70 kDa heat - shock protein (hsp70) in the Nile tilapia, Oreochromis niloticus, was investigated. The accumulation of hsp70 was determined in tissues of the liver, gills, spleen and heart of heat shocked fish. Other specimens were also exposed for 96 hr to four concentrations (1,0, 0.5, 0.1 and 0.001 mg/1) of Cu as copper sulphate and the accumulation of hsp70 was determined in the liver and gills. In both experiments, antibodies raised against hsp70 were employed as probes for dot-blot analysis and immunobinding assay of tilapia tissues. Decreasingly, hsp70 was abundant in the spleen, gills, heart and iiver of heat shocked fish. Also, data showed greater hsp70 level in the liver and gills of fish exposed to elevated Cu concentrations than the that of controls. These data suggest that hsp70 accumulation may hold a promise as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.
Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different ... more Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different localities in Qalyobya governorate, i.e., unpolluted, agricultural-polluted and industrial-polluted waters of River Nile. Water analysis was carried out to evaluate the heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution. The current study aims to revealing the effect of the water pollution, both agricultural and industerial, on the proteinogram and DNA damage in fish inhabiting these polluted areas of River Nile. The study suggested that there is clear molecular changes in T. zillii; this declares the use of these molecular parameters in diagnosing pollution.
Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egyp... more Profenofos as an organophosphorus insecticide has been used in the agricultural countries as Egypt, may find its way to water system and adversely effect on aquatic life particularly fish. Nile tilapia, Oreochromis niloticus as a major fish species in River Nile and one of the major sources of protein for human beings in Egypt, and it can also be a source of threaten to human health. Transport profenofos directly to tilapia fish may affect their physiological status and then fish production. The mortality of profenofos toxicity was estimated on tilapia and LC50 was detected as 0.87 mg/l. Also fish were exposed to 1/2 LC50 for 96 hrs and to 1/10 LC50 for 28 days and lastly were left after the chronic toxicity for another 28 days as recovery period. The increase of blood glucose was accompanied with decrease in liver and muscles glycogen throughout the acute and chronic trail periods. Fish showed also highly significant decrease in serum total protein and globulin with increasing in albumin and A/G ratio. A sharp elevation in serum creatinine, urea and uric acid with decrease in serum total lipid, triglyceride and cholesterol were also recorded. Lastly gradual and sharp elevation in the levels of serum enzymes, S-AST, S-ALT and S-ALP was revealed in profenofos-exposed tilapia. Same behavior as S-AST and S-ALT were in liver transaminases (L-AST & L-ALT). Our study revealed adverse change of metabolism in tilapia due to profenofos exposure. This may inform about the dangerous use of profenofos and limitations should be managed.
Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. I... more Shanawan drainage canal is one of the many drainage canals present in Menufiya province, Egypt. It receives sewage water from Kafr Shanawan drainage canal. Also, it receive illegal run–off from sewage and wastes besides sewage of Shebeen Al-koom city. This study is aimed to investigate the frequency of chromosomal damage by using chromosomal aberrations test of head kidney cells in Oreochromis niloticus and Tilapia zillii fish. The study was performed on 5 groups of fish, control and four polluted groups. A and B stations are located before the source of sewage discharge while C and D stations are located after the source of sewage. In each group cytological studies were performed. Fishes caught from areas before the sources of sewage showing different types of chromosomal aberrations, including centromeric attenuations, chromatid breaks, chromatid gabs, chromatid deletion, centric fusion and fragmentation. The total aberrations were more obvious among the fishes of the areas after the source of sewage than those of the areas before the source of sewage. It was noted that O. niloticus is more sensitive to the effect of pollution than T. zillii. Cytogenetic Studies of the current Work could be used as criteria for pollution intensity, which can be used to avoid its toxic effect on aquatic environment.
The present study was carried out to investigate the effect of sublethal zinc (Zn)
concentrations... more The present study was carried out to investigate the effect of sublethal zinc (Zn) concentrations on the growth, biochemical variables, and Zn residues in common carp, Cyprinus carpio L.. Fish (18.1 – 19.1 g) were exposed to 0.0 (control), 5.0 and 10.0 mg Zn/L for 7, 14, 28, and 56 days. At each time interval and each treatment, fish were collected, weighed and sampled to measure the growth, biochemical variables, and Zn residues in whole-fish body. Growth performance was significantly reduced with increasing Zn concentrations. However, fish exposed to 10.0 mg Zn/L for 56 days grew lower than that of the control group. Likewise, the optimum feed intake and feed conversion ratio were obtained at control group at 56 days. Furthermore, glucose, AST, ALT, creatinine, and cortisol increased significantly with increasing Zn concentration and exposure time, with maximal values at 56 days. Meanwhile, the highest values of serum protein and lipids of were obtained in the control fish reared for 56 days, whereas the lowest values were observed in fish exposed to 10.0 mg Zn/L for 56 days. The content of whole-body moisture and total ash increased significantly, while crude protein and total lipid contents decreased significantly with increasing Zn concentrations. In addition, Zn exposure increased Zn residues in fish body; however, Zn bioaccumulation in fish body was Zn dose and time dependant. The present study revealed that the growth and health status of common carp were deteriorated by Zn toxicity.
The present study deals with the effect of some water pollutants; copper sulfate as a fungicide, ... more The present study deals with the effect of some water pollutants; copper sulfate as a fungicide, malathion as an insecticide and paraquat as a herbicide, on ;:'_rum total protein serum albumin and globulin. Furthermore, proteinogram of blood serum niloticus in Oreochromis was done using electrophoresis. It was found that the control fish have nine serum protein fractions. The exposure of fish to the examined pollutants induced a disappearance of some fractions and consequently changes of relative mobility and area that indicate genetic damage.
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Papers by خالد شرف الدين
These data suggest that hsp70 accumulation may hold a promise as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.
heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in
the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution.
The current study aims to revealing the effect of the water pollution, both agricultural and industerial, on the proteinogram and DNA damage in fish inhabiting these polluted areas of River Nile.
The study suggested that there is clear molecular changes in T. zillii; this declares the use of these molecular parameters in diagnosing pollution.
chromosomal damage by using chromosomal aberrations test of head kidney cells in Oreochromis niloticus and Tilapia zillii fish. The study was performed on 5 groups of fish, control and four polluted groups. A and B stations are located before the source of sewage discharge while C and D stations are located after the source of sewage. In each group cytological studies were performed. Fishes caught from areas before the sources of sewage showing different types of chromosomal aberrations, including centromeric attenuations, chromatid breaks, chromatid gabs, chromatid deletion, centric fusion and fragmentation. The total aberrations were more obvious among the fishes of the areas after the source of sewage than those of the areas before the source of sewage. It was noted that O. niloticus is more sensitive to the effect of pollution than T. zillii. Cytogenetic Studies of the current Work could be used as criteria for pollution intensity, which can be used to avoid its toxic effect on aquatic environment.
concentrations on the growth, biochemical variables, and Zn residues in common
carp, Cyprinus carpio L.. Fish (18.1 – 19.1 g) were exposed to 0.0 (control), 5.0 and
10.0 mg Zn/L for 7, 14, 28, and 56 days. At each time interval and each treatment,
fish were collected, weighed and sampled to measure the growth, biochemical
variables, and Zn residues in whole-fish body. Growth performance was significantly
reduced with increasing Zn concentrations. However, fish exposed to 10.0 mg Zn/L
for 56 days grew lower than that of the control group. Likewise, the optimum feed
intake and feed conversion ratio were obtained at control group at 56 days.
Furthermore, glucose, AST, ALT, creatinine, and cortisol increased significantly with
increasing Zn concentration and exposure time, with maximal values at 56 days.
Meanwhile, the highest values of serum protein and lipids of were obtained in the
control fish reared for 56 days, whereas the lowest values were observed in fish
exposed to 10.0 mg Zn/L for 56 days. The content of whole-body moisture and total
ash increased significantly, while crude protein and total lipid contents decreased
significantly with increasing Zn concentrations. In addition, Zn exposure increased Zn
residues in fish body; however, Zn bioaccumulation in fish body was Zn dose and
time dependant. The present study revealed that the growth and health status of
common carp were deteriorated by Zn toxicity.
These data suggest that hsp70 accumulation may hold a promise as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.
heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in
the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution.
The current study aims to revealing the effect of the water pollution, both agricultural and industerial, on the proteinogram and DNA damage in fish inhabiting these polluted areas of River Nile.
The study suggested that there is clear molecular changes in T. zillii; this declares the use of these molecular parameters in diagnosing pollution.
chromosomal damage by using chromosomal aberrations test of head kidney cells in Oreochromis niloticus and Tilapia zillii fish. The study was performed on 5 groups of fish, control and four polluted groups. A and B stations are located before the source of sewage discharge while C and D stations are located after the source of sewage. In each group cytological studies were performed. Fishes caught from areas before the sources of sewage showing different types of chromosomal aberrations, including centromeric attenuations, chromatid breaks, chromatid gabs, chromatid deletion, centric fusion and fragmentation. The total aberrations were more obvious among the fishes of the areas after the source of sewage than those of the areas before the source of sewage. It was noted that O. niloticus is more sensitive to the effect of pollution than T. zillii. Cytogenetic Studies of the current Work could be used as criteria for pollution intensity, which can be used to avoid its toxic effect on aquatic environment.
concentrations on the growth, biochemical variables, and Zn residues in common
carp, Cyprinus carpio L.. Fish (18.1 – 19.1 g) were exposed to 0.0 (control), 5.0 and
10.0 mg Zn/L for 7, 14, 28, and 56 days. At each time interval and each treatment,
fish were collected, weighed and sampled to measure the growth, biochemical
variables, and Zn residues in whole-fish body. Growth performance was significantly
reduced with increasing Zn concentrations. However, fish exposed to 10.0 mg Zn/L
for 56 days grew lower than that of the control group. Likewise, the optimum feed
intake and feed conversion ratio were obtained at control group at 56 days.
Furthermore, glucose, AST, ALT, creatinine, and cortisol increased significantly with
increasing Zn concentration and exposure time, with maximal values at 56 days.
Meanwhile, the highest values of serum protein and lipids of were obtained in the
control fish reared for 56 days, whereas the lowest values were observed in fish
exposed to 10.0 mg Zn/L for 56 days. The content of whole-body moisture and total
ash increased significantly, while crude protein and total lipid contents decreased
significantly with increasing Zn concentrations. In addition, Zn exposure increased Zn
residues in fish body; however, Zn bioaccumulation in fish body was Zn dose and
time dependant. The present study revealed that the growth and health status of
common carp were deteriorated by Zn toxicity.