Scope of the present work is to infer the migratory ability of leukocytes by stochastic processes... more Scope of the present work is to infer the migratory ability of leukocytes by stochastic processes in order to distinguish the spontaneous organization of immune cells against an insult (namely cancer). For this purpose, spleen cells from immunodeficient mice, selectively lacking the transcription factor IRF-8 (IRF-8 knockout; IRF-8 KO), or from immunocompetent animals (wild-type; WT), were allowed to interact, alternatively, with murine B16.F10 melanoma cells in an ad hoc microfluidic environment developed on a LabOnChip technology. In this setting, onlyWTspleen cells were able to establish physical interactions with melanoma cells. Conversely, IRF-8 KO immune cells exhibited poor dynamical reactivity towards the neoplastic cells. In the present study, we collected data on the motility of these two types of spleen cells and built a complete set of observables that recapitulate the biological complexity of the system in these experiments. With remarkable accuracy, we concluded that the IRF-8KOcells performed pure uncorrelated random walks, while WT splenocytes were able to make singular drifted random walks that collapsed on a straight ballistic motion for the system as a whole, hence giving rise to a highly coordinate response. These results may provide a useful system to quantitatively analyse the real time cell-cell interactions and to foresee the behavior of immune cells with tumor cells at the tissue level.
ABSTRACT The use of superhydrophobic surfaces (SHSs) is now emerging as an attractive platform fo... more ABSTRACT The use of superhydrophobic surfaces (SHSs) is now emerging as an attractive platform for the realization of one-dimensional (1D) nanostructures with potential applications in many nanotechnological and biotechnological fields. To this purpose, a strict control of the nanostructures size and their spatial arrangement is highly required. However, these parameters may be strongly dependent on the complex evaporation dynamics of the sessile droplet on the SHS. In this work, we investigated the effect of the evaporation dynamics on the size and the spatial arrangement of self-assembled 1D DNA bundles. Our results reveal that different arrangements and bundle size distributions may occur depending on droplet evaporation stage. These results contribute to elucidate the formation mechanism of 1D nanostructures on SHSs.
Scope of the present work is to infer the migratory ability of leukocytes by stochastic processes... more Scope of the present work is to infer the migratory ability of leukocytes by stochastic processes in order to distinguish the spontaneous organization of immune cells against an insult (namely cancer). For this purpose, spleen cells from immunodeficient mice, selectively lacking the transcription factor IRF-8 (IRF-8 knockout; IRF-8 KO), or from immunocompetent animals (wild-type; WT), were allowed to interact, alternatively, with murine B16.F10 melanoma cells in an ad hoc microfluidic environment developed on a LabOnChip technology. In this setting, onlyWTspleen cells were able to establish physical interactions with melanoma cells. Conversely, IRF-8 KO immune cells exhibited poor dynamical reactivity towards the neoplastic cells. In the present study, we collected data on the motility of these two types of spleen cells and built a complete set of observables that recapitulate the biological complexity of the system in these experiments. With remarkable accuracy, we concluded that the IRF-8KOcells performed pure uncorrelated random walks, while WT splenocytes were able to make singular drifted random walks that collapsed on a straight ballistic motion for the system as a whole, hence giving rise to a highly coordinate response. These results may provide a useful system to quantitatively analyse the real time cell-cell interactions and to foresee the behavior of immune cells with tumor cells at the tissue level.
ABSTRACT The use of superhydrophobic surfaces (SHSs) is now emerging as an attractive platform fo... more ABSTRACT The use of superhydrophobic surfaces (SHSs) is now emerging as an attractive platform for the realization of one-dimensional (1D) nanostructures with potential applications in many nanotechnological and biotechnological fields. To this purpose, a strict control of the nanostructures size and their spatial arrangement is highly required. However, these parameters may be strongly dependent on the complex evaporation dynamics of the sessile droplet on the SHS. In this work, we investigated the effect of the evaporation dynamics on the size and the spatial arrangement of self-assembled 1D DNA bundles. Our results reveal that different arrangements and bundle size distributions may occur depending on droplet evaporation stage. These results contribute to elucidate the formation mechanism of 1D nanostructures on SHSs.
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Papers by Adele Ninno
distinguish the spontaneous organization of immune cells against an insult (namely cancer). For this
purpose, spleen cells from immunodeficient mice, selectively lacking the transcription factor IRF-8 (IRF-8
knockout; IRF-8 KO), or from immunocompetent animals (wild-type; WT), were allowed to interact,
alternatively, with murine B16.F10 melanoma cells in an ad hoc microfluidic environment developed on a
LabOnChip technology. In this setting, onlyWTspleen cells were able to establish physical interactions with
melanoma cells. Conversely, IRF-8 KO immune cells exhibited poor dynamical reactivity towards the
neoplastic cells. In the present study, we collected data on the motility of these two types of spleen cells and
built a complete set of observables that recapitulate the biological complexity of the system in these
experiments. With remarkable accuracy, we concluded that the IRF-8KOcells performed pure uncorrelated
random walks, while WT splenocytes were able to make singular drifted random walks that collapsed on a
straight ballistic motion for the system as a whole, hence giving rise to a highly coordinate response. These
results may provide a useful system to quantitatively analyse the real time cell-cell interactions and to foresee
the behavior of immune cells with tumor cells at the tissue level.
distinguish the spontaneous organization of immune cells against an insult (namely cancer). For this
purpose, spleen cells from immunodeficient mice, selectively lacking the transcription factor IRF-8 (IRF-8
knockout; IRF-8 KO), or from immunocompetent animals (wild-type; WT), were allowed to interact,
alternatively, with murine B16.F10 melanoma cells in an ad hoc microfluidic environment developed on a
LabOnChip technology. In this setting, onlyWTspleen cells were able to establish physical interactions with
melanoma cells. Conversely, IRF-8 KO immune cells exhibited poor dynamical reactivity towards the
neoplastic cells. In the present study, we collected data on the motility of these two types of spleen cells and
built a complete set of observables that recapitulate the biological complexity of the system in these
experiments. With remarkable accuracy, we concluded that the IRF-8KOcells performed pure uncorrelated
random walks, while WT splenocytes were able to make singular drifted random walks that collapsed on a
straight ballistic motion for the system as a whole, hence giving rise to a highly coordinate response. These
results may provide a useful system to quantitatively analyse the real time cell-cell interactions and to foresee
the behavior of immune cells with tumor cells at the tissue level.