Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in ou... more Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in outer membrane preparations from Leptospira pomona serovar mozdok. The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosic vaccine vax-SPIRAL ® in outer membrane proteins preparations from Leptospira pomoma serovar mozdok was made. In this paper we are beginning with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes- Edward method. In addition we carried out the characterization of obtained fractions, the molecular weight of the protein present in this fractions and its immunogenic capacity by western and dot blot were determinate. Ours results shows that this method is not adequate to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 principal bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 lessening bans such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolizacaride. Dot and western blot techniques showed the recognition of these proteins by the sera from individuals vaccinated. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 y 17.2 kDa.
The preliminary identification of antigens recognized by sera from individuals vaccinated with th... more The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosis vaccine vax-SPIRAL ® in outer membrane protein preparations from Leptospira pomoma serovar mozdok was made. In this paper we start with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes-Edward method. In addition, we characterized the obtained fractions; the molecular weight of the protein present in this fraction and its immunogenic capacity by western and dot blot were determined. Our results show that this is an appropriate method to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 main bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 minor bands such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolysaccharide. Dot and western blot techniques showed the recognition of these proteins by the sera from vaccinated individuals. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 and 17.2 kDa.
Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in ou... more Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in outer membrane preparations from Leptospira pomona serovar mozdok. The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosic vaccine vax-SPIRAL ® in outer membrane proteins preparations from Leptospira pomoma serovar mozdok was made. In this paper we are beginning with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes- Edward method. In addition we carried out the characterization of obtained fractions, the molecular weight of the protein present in this fractions and its immunogenic capacity by western and dot blot were determinate. Ours results shows that this method is not adequate to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 principal bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 lessening bans such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolizacaride. Dot and western blot techniques showed the recognition of these proteins by the sera from individuals vaccinated. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 y 17.2 kDa.
The preliminary identification of antigens recognized by sera from individuals vaccinated with th... more The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosis vaccine vax-SPIRAL ® in outer membrane protein preparations from Leptospira pomoma serovar mozdok was made. In this paper we start with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes-Edward method. In addition, we characterized the obtained fractions; the molecular weight of the protein present in this fraction and its immunogenic capacity by western and dot blot were determined. Our results show that this is an appropriate method to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 main bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 minor bands such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolysaccharide. Dot and western blot techniques showed the recognition of these proteins by the sera from vaccinated individuals. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 and 17.2 kDa.
... Figura 3. Estructura a plazo de las tasas de interés en Colombia y movimientos de la tasa de ... more ... Figura 3. Estructura a plazo de las tasas de interés en Colombia y movimientos de la tasa de intervención 4% ... 5 en las tasas de intervención, se utiliza una relación de paridad descubierta de intereses con la economía de Estados Unidos, nuestro principal referente financiero. ...
Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in ou... more Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in outer membrane preparations from Leptospira pomona serovar mozdok. The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosic vaccine vax-SPIRAL ® in outer membrane proteins preparations from Leptospira pomoma serovar mozdok was made. In this paper we are beginning with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes- Edward method. In addition we carried out the characterization of obtained fractions, the molecular weight of the protein present in this fractions and its immunogenic capacity by western and dot blot were determinate. Ours results shows that this method is not adequate to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 principal bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 lessening bans such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolizacaride. Dot and western blot techniques showed the recognition of these proteins by the sera from individuals vaccinated. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 y 17.2 kDa.
The preliminary identification of antigens recognized by sera from individuals vaccinated with th... more The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosis vaccine vax-SPIRAL ® in outer membrane protein preparations from Leptospira pomoma serovar mozdok was made. In this paper we start with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes-Edward method. In addition, we characterized the obtained fractions; the molecular weight of the protein present in this fraction and its immunogenic capacity by western and dot blot were determined. Our results show that this is an appropriate method to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 main bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 minor bands such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolysaccharide. Dot and western blot techniques showed the recognition of these proteins by the sera from vaccinated individuals. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 and 17.2 kDa.
Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in ou... more Preliminary characterization of the antigens recognized by sera from vax-SPIRAL ® vaccinees in outer membrane preparations from Leptospira pomona serovar mozdok. The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosic vaccine vax-SPIRAL ® in outer membrane proteins preparations from Leptospira pomoma serovar mozdok was made. In this paper we are beginning with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes- Edward method. In addition we carried out the characterization of obtained fractions, the molecular weight of the protein present in this fractions and its immunogenic capacity by western and dot blot were determinate. Ours results shows that this method is not adequate to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 principal bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 lessening bans such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolizacaride. Dot and western blot techniques showed the recognition of these proteins by the sera from individuals vaccinated. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 y 17.2 kDa.
The preliminary identification of antigens recognized by sera from individuals vaccinated with th... more The preliminary identification of antigens recognized by sera from individuals vaccinated with the antileptospirosis vaccine vax-SPIRAL ® in outer membrane protein preparations from Leptospira pomoma serovar mozdok was made. In this paper we start with the solubilization of the outer membrane components of Leptospira interrogans serovar mozdok, using the Nunes-Edward method. In addition, we characterized the obtained fractions; the molecular weight of the protein present in this fraction and its immunogenic capacity by western and dot blot were determined. Our results show that this is an appropriate method to identify antigens for vaccine purposes. Humoral response was addressed to antigens absents in the outer membrane preparations with the aforementioned method. Coomassie stain showed a profile characterised by 16 bands; 6 main bands, 49.4, 45.8, 36.7, 28.3 and 21.0 kDa and 10 minor bands such as 66.4, 57.2, 34.1 y 32.8 kDa. The silver stain showed the presence of lipopolysaccharide. Dot and western blot techniques showed the recognition of these proteins by the sera from vaccinated individuals. The humoral response was addressed mainly to the bands of 66.1, 58.3, 47.2, 45.3, 34.5, 30.0, 29.8 and 17.2 kDa.
... Figura 3. Estructura a plazo de las tasas de interés en Colombia y movimientos de la tasa de ... more ... Figura 3. Estructura a plazo de las tasas de interés en Colombia y movimientos de la tasa de intervención 4% ... 5 en las tasas de intervención, se utiliza una relación de paridad descubierta de intereses con la economía de Estados Unidos, nuestro principal referente financiero. ...
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Papers by Andres Felipe Gonzalez