Induction of adventitious buds and shoots from intact leaves and stem internode segments of two r... more Induction of adventitious buds and shoots from intact leaves and stem internode segments of two recalcitrant Ugandan sweetpotato (Ipomoea batatas L.) cultivars was investigated in vitro on Murashige and Skoog (MS) medium, supplemented with 3 different levels (0.5, 2.0 and 4.0 µM) of Thidiazuron (TDZ). Shoots were regenerated in all TDZ concentrations in cvs. Kyebandula and Bwanjule. The inclusion of 0.25 µM α-Naphthalene acetic acid (NAA) in MS medium, containing TDZ (0.5 µM), improved shoot regeneration frequency from 12.1 to 22.6 % for cv. Kyebandula stems and from 21.61 to 42.9 % for cv. Bwanjule stems. However, there was about 10 % reduction in adventitious bud induction frequency for both cultivars, when NAA was included in the medium. The highest frequency (66.7%) of adventitious bud induction was achieved from stem explants of cv. Kyebandula. The conversion of adventitious buds into shoots was improved when TDZ was reduced or completely removed in subsequent stages of culture...
Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investiga... more Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investigated sweet potato cultivars on Murashige and Skoog (1962) medium supplemented with 2,4 Dichlorophenoxyacetic acid (0.2 mg/L) at the Makerere University Agricultural Research Institute Tissue culture laboratory. Shoot and root regeneration was possible in the non-African cultivar, Jonathan, whereas only root regeneration was successfully induced in five African cultivars. Cultivar type had a highly significant (P < 0.001) effect on frequency of embryo-like structures and efficiency of both shoot and root regeneration. The embryo-like structures induced could be useful for initiation of cell suspensions to enable genetic transformation of African cultivars. The method for de novo regeneration of roots has potential application in the regeneration of plants or hairy root cultures for cultivars that are recalcitrant to shoot regeneration.  Key words: 2, 4-dichlorophenoxyacetic acid, culti...
During the period 2002-10, FAO undertook an intense activity of biosafety capacity development, l... more During the period 2002-10, FAO undertook an intense activity of biosafety capacity development, largely centred on enhancing the capacities of regulators and other technical staff involved in the implementation of biosafety frameworks, along with other components. The training programme was tailored to meet the needs of a very specific audience: biosafety regulators, policy-makers and members of national biosafety committees, with diverse educational backgrounds, not necessarily well versed in all the biosafety-related fields. The training courses therefore aimed to: i) offer background knowledge critical in the process of reviewing biosafety dossiers and biosafety-related decision-making ; ii) provide acquaintance with concepts and methodologies relevant to risk analysis of genetically modified organism (GMO) release and biosafety management. The training programme consisted of the following modules: Module A: Introduction to molecular biology and genetic engineering. This reviews ...
A dissertation submitted to the school of graduate studies in partial fulfilment of the requireme... more A dissertation submitted to the school of graduate studies in partial fulfilment of the requirements for the award of doctor of philosophy in plant breeding and biotechnology of Makerere University
Induction of adventitious buds and shoots from intact leaves and stem internode segments of two r... more Induction of adventitious buds and shoots from intact leaves and stem internode segments of two recalcitrant Ugandan sweetpotato (Ipomoea batatas L.) cultivars was investigated in vitro on Murashige and Skoog (MS) medium, supplemented with 3 different levels (0.5, 2.0 and 4.0 µM) of Thidiazuron (TDZ). Shoots were regenerated in all TDZ concentrations in cvs. Kyebandula and Bwanjule. The inclusion of 0.25 µM α-Naphthalene acetic acid (NAA) in MS medium, containing TDZ (0.5 µM), improved shoot regeneration frequency from 12.1 to 22.6 % for cv. Kyebandula stems and from 21.61 to 42.9 % for cv. Bwanjule stems. However, there was about 10 % reduction in adventitious bud induction frequency for both cultivars, when NAA was included in the medium. The highest frequency (66.7%) of adventitious bud induction was achieved from stem explants of cv. Kyebandula. The conversion of adventitious buds into shoots was improved when TDZ was reduced or completely removed in subsequent stages of culture...
Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investiga... more Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investigated sweet potato cultivars on Murashige and Skoog (1962) medium supplemented with 2,4 Dichlorophenoxyacetic acid (0.2 mg/L) at the Makerere University Agricultural Research Institute Tissue culture laboratory. Shoot and root regeneration was possible in the non-African cultivar, Jonathan, whereas only root regeneration was successfully induced in five African cultivars. Cultivar type had a highly significant (P < 0.001) effect on frequency of embryo-like structures and efficiency of both shoot and root regeneration. The embryo-like structures induced could be useful for initiation of cell suspensions to enable genetic transformation of African cultivars. The method for de novo regeneration of roots has potential application in the regeneration of plants or hairy root cultures for cultivars that are recalcitrant to shoot regeneration.  Key words: 2, 4-dichlorophenoxyacetic acid, culti...
During the period 2002-10, FAO undertook an intense activity of biosafety capacity development, l... more During the period 2002-10, FAO undertook an intense activity of biosafety capacity development, largely centred on enhancing the capacities of regulators and other technical staff involved in the implementation of biosafety frameworks, along with other components. The training programme was tailored to meet the needs of a very specific audience: biosafety regulators, policy-makers and members of national biosafety committees, with diverse educational backgrounds, not necessarily well versed in all the biosafety-related fields. The training courses therefore aimed to: i) offer background knowledge critical in the process of reviewing biosafety dossiers and biosafety-related decision-making ; ii) provide acquaintance with concepts and methodologies relevant to risk analysis of genetically modified organism (GMO) release and biosafety management. The training programme consisted of the following modules: Module A: Introduction to molecular biology and genetic engineering. This reviews ...
A dissertation submitted to the school of graduate studies in partial fulfilment of the requireme... more A dissertation submitted to the school of graduate studies in partial fulfilment of the requirements for the award of doctor of philosophy in plant breeding and biotechnology of Makerere University
Uploads
Papers by Andrew Kiggundu