ABSTRACT Wood-decay macro-fungi have been reported to be a major cause of decline of arid zone tr... more ABSTRACT Wood-decay macro-fungi have been reported to be a major cause of decline of arid zone trees in the genera Acacia, Prosopis, Ziziphus and Azadirachta; the identity of these fungi, however, has not been confirmed in the state of Rajasthan in northwestern India. Direct sequencing of PCR amplified ITS regions of rDNA facilitated molecular identification of 26 isolates into species in eight genera (Ganoderma, Inonotus, Phellinus, Ceriporia, Schizophyllum, Phanerochaete, Pleurotus, Leucoagaricus) and one incompletely characterized Basidiomycota species. In addition to intra-specific phenotypic variations in basidiocarp morphology, Single Nucleotide Polymorphisms (SNPs) were recorded among isolates of P. pulmonarius, S. commune, G. lucidum and Ganoderma species. Both SNPs and insertions/deletions (INDELS) were recorded amongst Inonotus species. A phylogram based on multiple sequence alignment delineated all isolates into separate intra-specific subgroups along with their GenBank reference sequences with high bootstrap values. Phellinus repandus, L. meleagris, C. lacerata and three Inonotus species (I. rickii, I. patouillardii, and I. porrectus) reported herein are new fungal records from India.
ABSTRACT Wood-decay macro-fungi have been reported to be a major cause of decline of arid zone tr... more ABSTRACT Wood-decay macro-fungi have been reported to be a major cause of decline of arid zone trees in the genera Acacia, Prosopis, Ziziphus and Azadirachta; the identity of these fungi, however, has not been confirmed in the state of Rajasthan in northwestern India. Direct sequencing of PCR amplified ITS regions of rDNA facilitated molecular identification of 26 isolates into species in eight genera (Ganoderma, Inonotus, Phellinus, Ceriporia, Schizophyllum, Phanerochaete, Pleurotus, Leucoagaricus) and one incompletely characterized Basidiomycota species. In addition to intra-specific phenotypic variations in basidiocarp morphology, Single Nucleotide Polymorphisms (SNPs) were recorded among isolates of P. pulmonarius, S. commune, G. lucidum and Ganoderma species. Both SNPs and insertions/deletions (INDELS) were recorded amongst Inonotus species. A phylogram based on multiple sequence alignment delineated all isolates into separate intra-specific subgroups along with their GenBank reference sequences with high bootstrap values. Phellinus repandus, L. meleagris, C. lacerata and three Inonotus species (I. rickii, I. patouillardii, and I. porrectus) reported herein are new fungal records from India.
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