The origin of replication of Escherichia coli, oriC, has been labelled by fluorescent in situ hyb... more The origin of replication of Escherichia coli, oriC, has been labelled by fluorescent in situ hybridisation (FISH). The E. coli K12 strain was grown under steady state conditions with a doubling time of 79 min at 28 °C. Under these growth conditions DNA replication starts in the previous cell cycle at -33 min. At birth cells possess two origins, which are visible as two separated foci in fully labelled cells. The number of foci increased with cell length. The distance of foci from the nearest cell pole has been
Autosomal dominant polycystic kidney disease (ADPKD) is a very common inherited disease caused by... more Autosomal dominant polycystic kidney disease (ADPKD) is a very common inherited disease caused by mutations in PKD1 or PKD2 genes characterized by progressive enlargement of fluid-filled cysts and loss of renal function [1]. Previous studies proposed a role for human polycystin-1 in renal morphogenesis acting as a matrix receptor in focal adhesions and for polycystin-2 as a putative calcium channel [2, 3]. The genome of Caenorhabditis elegans contains 2 new members of the polycystin family: lov-1, the homolog for PKD1; and pkd-2, the homolog for PKD2 [4; this paper]. Mutation analysis in C. elegans showed similarly compromised male mating behaviors in all single and double lov-1 and pkd-2 mutants, indicating their participation in a single genetic pathway. Expression analysis localized LOV-1 and PKD-2 to the ends of sensory neurons in male tails and to the tips of CEM neurons in the head, consistent with functions as chemo- or mechanosensors. Human and C. elegans PKD1 and PKD2 homologs, transfected into mammalian renal epithelial cells, co-localized with paxillin in focal adhesions suggesting function in a single biological pathway. Based on the role of polycystins in C. elegans sensory neuron function and the conservation of PKD pathways we suggest that polycystins act as sensors of the extracellular environment, initiating, via focal adhesion assembly, intracellular transduction events in neuronal or morphogenetic processes.
The origin of replication of Escherichia coli, oriC, has been labelled by fluorescent in situ hyb... more The origin of replication of Escherichia coli, oriC, has been labelled by fluorescent in situ hybridisation (FISH). The E. coli K12 strain was grown under steady state conditions with a doubling time of 79 min at 28 °C. Under these growth conditions DNA replication starts in the previous cell cycle at -33 min. At birth cells possess two origins, which are visible as two separated foci in fully labelled cells. The number of foci increased with cell length. The distance of foci from the nearest cell pole has been
Autosomal dominant polycystic kidney disease (ADPKD) is a very common inherited disease caused by... more Autosomal dominant polycystic kidney disease (ADPKD) is a very common inherited disease caused by mutations in PKD1 or PKD2 genes characterized by progressive enlargement of fluid-filled cysts and loss of renal function [1]. Previous studies proposed a role for human polycystin-1 in renal morphogenesis acting as a matrix receptor in focal adhesions and for polycystin-2 as a putative calcium channel [2, 3]. The genome of Caenorhabditis elegans contains 2 new members of the polycystin family: lov-1, the homolog for PKD1; and pkd-2, the homolog for PKD2 [4; this paper]. Mutation analysis in C. elegans showed similarly compromised male mating behaviors in all single and double lov-1 and pkd-2 mutants, indicating their participation in a single genetic pathway. Expression analysis localized LOV-1 and PKD-2 to the ends of sensory neurons in male tails and to the tips of CEM neurons in the head, consistent with functions as chemo- or mechanosensors. Human and C. elegans PKD1 and PKD2 homologs, transfected into mammalian renal epithelial cells, co-localized with paxillin in focal adhesions suggesting function in a single biological pathway. Based on the role of polycystins in C. elegans sensory neuron function and the conservation of PKD pathways we suggest that polycystins act as sensors of the extracellular environment, initiating, via focal adhesion assembly, intracellular transduction events in neuronal or morphogenetic processes.
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