vol. 12, no. 1, January 2015 by Bela R
Rice is a staple food to almost one-third population of the world. The crop sustainability must t... more Rice is a staple food to almost one-third population of the world. The crop sustainability must therefore be considered
to keep pace with the growing world population. Microorganims are one of the key factors affecting crop productivity
and quality. Of particular interest is the plant rhiszosphere microbiome which has gained enormous attention in recent
years due to their role both as pathogen and plant growth promoting agents. Accordingly, the present study is aimed at
identification of bacteria isolated from soil samples collected from rice fields of district Jaffarabad, which belongs to
the major rice growing area of Pakistan. Six strains B9, B15, B34, B40, B52 and B53 were identified as genus Bacillus
whereas isolate B12 as genus Staphylococcus based on morphological and biochemical properties and 16S rRNA gene
sequence analysis. Isolates B9, B40 and B53 showed 99% similarity to Bacillus thuringiensis, Bacillus anthracis and
Bacillus endophyticus respectively. Conflicting results were observed for B15, B34 and B52 for sequence analysis
based on BLAST and SeqMatch alignment. The isolate B12 showed 99% similarity to Staphylococcus aureus. Our
findings indicate that instead of resorting rice bacterial populations through biochemical tests, the validation through
16S rRNA technique provides a more objective view.
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vol. 12, no. 1, January 2015 by Bela R
to keep pace with the growing world population. Microorganims are one of the key factors affecting crop productivity
and quality. Of particular interest is the plant rhiszosphere microbiome which has gained enormous attention in recent
years due to their role both as pathogen and plant growth promoting agents. Accordingly, the present study is aimed at
identification of bacteria isolated from soil samples collected from rice fields of district Jaffarabad, which belongs to
the major rice growing area of Pakistan. Six strains B9, B15, B34, B40, B52 and B53 were identified as genus Bacillus
whereas isolate B12 as genus Staphylococcus based on morphological and biochemical properties and 16S rRNA gene
sequence analysis. Isolates B9, B40 and B53 showed 99% similarity to Bacillus thuringiensis, Bacillus anthracis and
Bacillus endophyticus respectively. Conflicting results were observed for B15, B34 and B52 for sequence analysis
based on BLAST and SeqMatch alignment. The isolate B12 showed 99% similarity to Staphylococcus aureus. Our
findings indicate that instead of resorting rice bacterial populations through biochemical tests, the validation through
16S rRNA technique provides a more objective view.
to keep pace with the growing world population. Microorganims are one of the key factors affecting crop productivity
and quality. Of particular interest is the plant rhiszosphere microbiome which has gained enormous attention in recent
years due to their role both as pathogen and plant growth promoting agents. Accordingly, the present study is aimed at
identification of bacteria isolated from soil samples collected from rice fields of district Jaffarabad, which belongs to
the major rice growing area of Pakistan. Six strains B9, B15, B34, B40, B52 and B53 were identified as genus Bacillus
whereas isolate B12 as genus Staphylococcus based on morphological and biochemical properties and 16S rRNA gene
sequence analysis. Isolates B9, B40 and B53 showed 99% similarity to Bacillus thuringiensis, Bacillus anthracis and
Bacillus endophyticus respectively. Conflicting results were observed for B15, B34 and B52 for sequence analysis
based on BLAST and SeqMatch alignment. The isolate B12 showed 99% similarity to Staphylococcus aureus. Our
findings indicate that instead of resorting rice bacterial populations through biochemical tests, the validation through
16S rRNA technique provides a more objective view.