Journal of Strength and Conditioning Research, 2016
In order to test the influence of probiotic supplementation on humoral immune response a double-b... more In order to test the influence of probiotic supplementation on humoral immune response a double-blind placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: VO2max 38.2 ± 4.9 ml/kg/min, age 23.2 ± 1.4 years; males: VO2max 57.5 ± 9.2 ml/kg/min, age 24.0 ± 2.4 years, mean ± SD) were randomized either to the probiotic group (L. helveticus Lafti® L10, 2 x 10 CFU), or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific anti-bacterial antibody levels of IgM, IgG and IgA classes were determined towards different bacteria in the serum, while in saliva total and specific anti-bacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15 to 20%; mean, 90% confidence interval; p=0.02) and placebo group (35%, 22 to 47%; p=0.02), without observed differences in changes between the groups. No significant changes in IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, -2.8 to 35%, p=0.04) reduction of anti - Enterococcus faecalis IgG was noted in the placebo group, in comparation to the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12 to 18%, p=0.04) or in saliva (35%, -1.4 to 53%, p=0.03). The significantly reduced levels of serum anti-LAB IgA antibodies in the placebo group compared to the probiotic group were detected for L. rhamnosus LA68 for (24%, 5.8 to 42%, p=0.02) and for L. rhamnosus LB64 for (15%, 2.7 to 27%, p=0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses.
Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences
The aim of present study was to assess the effects and safety of a dry Phaseoli vulgari pericarpi... more The aim of present study was to assess the effects and safety of a dry Phaseoli vulgari pericarpium (PVP) extract on postprandial glycemia in healthy participants. A randomized crossover experiment where participants received either PVP extract or placebo. Chemical compounds in dry extract were assessed by established methods. Eighteen healthy participants (9 male and 9 female) aged 29+/-4,8 years, body mass index (BMI) 23+/-3,7 kg/m(2) were recruited among students and staff at the Faculty of Pharmacy, University of Belgrade. All participants were able to follow the study protocol without difficulty. The participants received either PVP extract or placebo 30 minutes before a 50g oral glucose tolerance test (OGTT). The protocol followed the guidelines for the OGTT with blood samples drawn at 0, 15, 30, 60, 90 and 120 min. This study demonstrated that there was no significantly effect of the PVP extract on incremental blood glucose (IBG) and their areas under the curve (AUC) neither ...
ABSTRACT Fruits and vegetables are known as good sources of numerous bioactive compounds among wh... more ABSTRACT Fruits and vegetables are known as good sources of numerous bioactive compounds among which polyphenols and dietary fibre are considered essential because of their protective health effects. The aim of this study was to characterize the quality of selected plant foods of Serbia regarding the amount of total phenols, fibres and ratio of certain fractions of fibre. Fifteen samples of plant foods (green leafy vegetables, fruits and nuts) were evaluated for their total antioxidant activity, total phenolic content, total, soluble and insoluble fibre and fractions of fibre: beta-glucans, arabinoxylan, cellulose and resistant starch. Generally, nuts were the richest sources of fibre and total phenols. However, when serving size was taken into consideration, it appeared that raspberry and blackberry were the richest in total, soluble fibre and cellulose. Furthermore, almonds and hazelnuts were particularly rich in insoluble fibre, while walnuts had the highest polyphenol content. The analyzed plant foods were poor sources of arabinoxylan and beta-glucan. Data on the presence of resistant starch in cashew nut was the first confirmation that resistant starch could be found in significant amount in some nuts. The results give rare insight into the quality of selected plant foods regarding dietary fibre and polyphenols from the nutritive point of view.
Starting from the evidence-based health benefits that resistant starch (RS) shows when added to t... more Starting from the evidence-based health benefits that resistant starch (RS) shows when added to the diet, our aim in this study was to evaluate the effects of increased fibre intake with two different levels of RS coming from regular daily consumed foods on normalization of glycaemia within lifestyle intervention in the population with risk factors for developing diabetes. Study included 47 overweight and obese men and women with disordered glucoregulation and dyslipidaemia, aged between 45-74, divided into RS and Fibre group. Participants were subjected to the lifestyle and dietary intervention with low-fat and high-fibre (>25 g/day) diet for 12 months and were offered two different dietary advices aimed at increasing total fibre intake in Fibre group and at increasing RS intake in RS group. The intake of macronutrients and total fibre was similar between groups at the end of the study, but achieved RS intake was two times higher in the RS group. Decrease in total cholesterol an...
The Journal of sports medicine and physical fitness, 2014
The aim of the study was to determine whether single soccer training is able to cause oxidative s... more The aim of the study was to determine whether single soccer training is able to cause oxidative stress and DNA instability. We have also sought to investigate if adaptive response will be developed during 45 days training period and to what extent. This study was conducted on 16 soccer players aged 18.13±0.35 years. We used single cells gel electrophoresis (comet assay) to investigate leukocyte DNA stability. The results were presented as DNA score and percent of cells with medium and high damage. Oxidative status of our subjects was estimated through blood levels of superoxide anion, the thiobarbituric acid-reacting substances (TBARs), total antioxidant status (TAS), total oxidant status (TOS), prooxidant-antioxidant balance (PAB) and sulfhydryl-groups (SH-groups). During single soccer training, DNA score and percent of the cells with medium and high damage were increased after training but without significance. Sulphydryl-groups (P=0.033), TOS (P=0.002) and PAB (P=0.045) were sign...
The Journal of sports medicine and physical fitness, 2012
The purpose of the current study was to examine the effect of Astaxanthin (Asx) supplementation o... more The purpose of the current study was to examine the effect of Astaxanthin (Asx) supplementation on muscle enzymes as indirect markers of muscle damage, oxidative stress markers and antioxidant response in elite young soccer players. Thirty-two male elite soccer players were randomly assigned in a double-blind fashion to Asx and placebo (P) group. After the 90 days of supplementation, the athletes performed a 2 hour acute exercise bout. Blood samples were obtained before and after 90 days of supplementation and after the exercise at the end of observational period for analysis of thiobarbituric acid-reacting substances (TBARS), advanced oxidation protein products (AOPP), superoxide anion (O2•¯), total antioxidative status (TAS), sulphydril groups (SH), superoxide-dismutase (SOD), serum creatine kinase (CK) and aspartate aminotransferase (AST). TBARS and AOPP levels did not change throughout the study. Regular training significantly increased O2•¯ levels (main training effect, P<0....
In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluate... more In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluated for their effects on high fat diet induced pathology in mice. The aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. C57BL/6 mice fed a high fat diet were orally administered with the Lactobacillus strains. Both the metabolic and immunological parameters were analyzed. The administration of both of the strains had beneficial effects on mouse weight, serum cholesterol, TNF-α levels and liver histology. LA68 lowered the total cholesterol and HDL levels more prominently, whereas WCFS1 was more potent in lowering the TG and LDL levels. Leptin and adiponectin levels were increased in all experimental groups to different extents. The administration of L. plantarum WCFS1 led to a marked increase in leptin levels, as well as an increase in CD3+CD4+ and CD3+CD8+ cells, and a decrease of CD25+ cells, and had a lowering effect...
The aim of this study was to determine the plasma selenium (Se), copper (Cu), and zinc (Zn) level... more The aim of this study was to determine the plasma selenium (Se), copper (Cu), and zinc (Zn) levels and to evaluate their possible association with metabolic syndrome (MetS) components in patients with schizophrenia. The study group consisted of 60 patients with schizophrenia and 60 sex- and age-matched healthy controls. Anthropometric measurements, blood pressure, and biochemical analysis of fasting blood were performed in all subjects. Patients with schizophrenia had significantly higher plasma Cu concentrations compared with controls (0.97 ± 0.31 vs. 0.77 ± 0.32 mg/L, p = 0.001). The plasma Cu concentration showed a positive correlation with plasma glucose and diastolic blood pressure in the patient groups (r s = 0.263, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05 and r s = 0.272, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05, respectively). The plasma Se level correlated positive with MetS score (r s = 0.385, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01), waist circumference (r s = 0.344, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), plasma glucose (r s = 0.319, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), and triglyceride concentrations (r s = 0.462, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001) in patients with schizophrenia. Plasma Zn did not correlate with any of the MetS components. These results suggest that alterations in plasma Cu and Se levels in medicated patients with schizophrenia could be associated with metabolic risk factors.
Scandinavian Journal of Clinical & Laboratory Investigation, 2014
The purpose of this study was to assess oxidative stress status parameters and their possible ass... more The purpose of this study was to assess oxidative stress status parameters and their possible associations with traditional cardiovascular risk factors in patients with schizophrenia, as well as their potential for patient-control discrimination. Fasting glucose, lipid profile and oxidative stress status parameters were assessed in 30 schizophrenic patients with atypical antipsychotic therapy and 60 control subjects. Malondialdehyde (MDA), pro-oxidant/antioxidant balance (PAB) and total anti-oxidant status (TAS) were significantly higher whereas total sulfhydryl (SH) groups were significantly lower in schizophrenic patients vs. control group. Higher serum PAB values showed an independent association with schizophrenia. The addition of PAB to conventional risk factors improved discrimination between healthy control subjects and patients. Increased oxidative stress and changed lipid profile parameters are associated in schizophrenic patients and may indicate risk for atherosclerosis. The serum PAB level may reflect the levels of oxidative stress in schizophrenia and improve discrimination of patients from controls.
Journal of Strength and Conditioning Research, 2016
In order to test the influence of probiotic supplementation on humoral immune response a double-b... more In order to test the influence of probiotic supplementation on humoral immune response a double-blind placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: VO2max 38.2 ± 4.9 ml/kg/min, age 23.2 ± 1.4 years; males: VO2max 57.5 ± 9.2 ml/kg/min, age 24.0 ± 2.4 years, mean ± SD) were randomized either to the probiotic group (L. helveticus Lafti® L10, 2 x 10 CFU), or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific anti-bacterial antibody levels of IgM, IgG and IgA classes were determined towards different bacteria in the serum, while in saliva total and specific anti-bacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15 to 20%; mean, 90% confidence interval; p=0.02) and placebo group (35%, 22 to 47%; p=0.02), without observed differences in changes between the groups. No significant changes in IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, -2.8 to 35%, p=0.04) reduction of anti - Enterococcus faecalis IgG was noted in the placebo group, in comparation to the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12 to 18%, p=0.04) or in saliva (35%, -1.4 to 53%, p=0.03). The significantly reduced levels of serum anti-LAB IgA antibodies in the placebo group compared to the probiotic group were detected for L. rhamnosus LA68 for (24%, 5.8 to 42%, p=0.02) and for L. rhamnosus LB64 for (15%, 2.7 to 27%, p=0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses.
Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences
The aim of present study was to assess the effects and safety of a dry Phaseoli vulgari pericarpi... more The aim of present study was to assess the effects and safety of a dry Phaseoli vulgari pericarpium (PVP) extract on postprandial glycemia in healthy participants. A randomized crossover experiment where participants received either PVP extract or placebo. Chemical compounds in dry extract were assessed by established methods. Eighteen healthy participants (9 male and 9 female) aged 29+/-4,8 years, body mass index (BMI) 23+/-3,7 kg/m(2) were recruited among students and staff at the Faculty of Pharmacy, University of Belgrade. All participants were able to follow the study protocol without difficulty. The participants received either PVP extract or placebo 30 minutes before a 50g oral glucose tolerance test (OGTT). The protocol followed the guidelines for the OGTT with blood samples drawn at 0, 15, 30, 60, 90 and 120 min. This study demonstrated that there was no significantly effect of the PVP extract on incremental blood glucose (IBG) and their areas under the curve (AUC) neither ...
ABSTRACT Fruits and vegetables are known as good sources of numerous bioactive compounds among wh... more ABSTRACT Fruits and vegetables are known as good sources of numerous bioactive compounds among which polyphenols and dietary fibre are considered essential because of their protective health effects. The aim of this study was to characterize the quality of selected plant foods of Serbia regarding the amount of total phenols, fibres and ratio of certain fractions of fibre. Fifteen samples of plant foods (green leafy vegetables, fruits and nuts) were evaluated for their total antioxidant activity, total phenolic content, total, soluble and insoluble fibre and fractions of fibre: beta-glucans, arabinoxylan, cellulose and resistant starch. Generally, nuts were the richest sources of fibre and total phenols. However, when serving size was taken into consideration, it appeared that raspberry and blackberry were the richest in total, soluble fibre and cellulose. Furthermore, almonds and hazelnuts were particularly rich in insoluble fibre, while walnuts had the highest polyphenol content. The analyzed plant foods were poor sources of arabinoxylan and beta-glucan. Data on the presence of resistant starch in cashew nut was the first confirmation that resistant starch could be found in significant amount in some nuts. The results give rare insight into the quality of selected plant foods regarding dietary fibre and polyphenols from the nutritive point of view.
Starting from the evidence-based health benefits that resistant starch (RS) shows when added to t... more Starting from the evidence-based health benefits that resistant starch (RS) shows when added to the diet, our aim in this study was to evaluate the effects of increased fibre intake with two different levels of RS coming from regular daily consumed foods on normalization of glycaemia within lifestyle intervention in the population with risk factors for developing diabetes. Study included 47 overweight and obese men and women with disordered glucoregulation and dyslipidaemia, aged between 45-74, divided into RS and Fibre group. Participants were subjected to the lifestyle and dietary intervention with low-fat and high-fibre (>25 g/day) diet for 12 months and were offered two different dietary advices aimed at increasing total fibre intake in Fibre group and at increasing RS intake in RS group. The intake of macronutrients and total fibre was similar between groups at the end of the study, but achieved RS intake was two times higher in the RS group. Decrease in total cholesterol an...
The Journal of sports medicine and physical fitness, 2014
The aim of the study was to determine whether single soccer training is able to cause oxidative s... more The aim of the study was to determine whether single soccer training is able to cause oxidative stress and DNA instability. We have also sought to investigate if adaptive response will be developed during 45 days training period and to what extent. This study was conducted on 16 soccer players aged 18.13±0.35 years. We used single cells gel electrophoresis (comet assay) to investigate leukocyte DNA stability. The results were presented as DNA score and percent of cells with medium and high damage. Oxidative status of our subjects was estimated through blood levels of superoxide anion, the thiobarbituric acid-reacting substances (TBARs), total antioxidant status (TAS), total oxidant status (TOS), prooxidant-antioxidant balance (PAB) and sulfhydryl-groups (SH-groups). During single soccer training, DNA score and percent of the cells with medium and high damage were increased after training but without significance. Sulphydryl-groups (P=0.033), TOS (P=0.002) and PAB (P=0.045) were sign...
The Journal of sports medicine and physical fitness, 2012
The purpose of the current study was to examine the effect of Astaxanthin (Asx) supplementation o... more The purpose of the current study was to examine the effect of Astaxanthin (Asx) supplementation on muscle enzymes as indirect markers of muscle damage, oxidative stress markers and antioxidant response in elite young soccer players. Thirty-two male elite soccer players were randomly assigned in a double-blind fashion to Asx and placebo (P) group. After the 90 days of supplementation, the athletes performed a 2 hour acute exercise bout. Blood samples were obtained before and after 90 days of supplementation and after the exercise at the end of observational period for analysis of thiobarbituric acid-reacting substances (TBARS), advanced oxidation protein products (AOPP), superoxide anion (O2•¯), total antioxidative status (TAS), sulphydril groups (SH), superoxide-dismutase (SOD), serum creatine kinase (CK) and aspartate aminotransferase (AST). TBARS and AOPP levels did not change throughout the study. Regular training significantly increased O2•¯ levels (main training effect, P<0....
In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluate... more In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluated for their effects on high fat diet induced pathology in mice. The aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. C57BL/6 mice fed a high fat diet were orally administered with the Lactobacillus strains. Both the metabolic and immunological parameters were analyzed. The administration of both of the strains had beneficial effects on mouse weight, serum cholesterol, TNF-α levels and liver histology. LA68 lowered the total cholesterol and HDL levels more prominently, whereas WCFS1 was more potent in lowering the TG and LDL levels. Leptin and adiponectin levels were increased in all experimental groups to different extents. The administration of L. plantarum WCFS1 led to a marked increase in leptin levels, as well as an increase in CD3+CD4+ and CD3+CD8+ cells, and a decrease of CD25+ cells, and had a lowering effect...
The aim of this study was to determine the plasma selenium (Se), copper (Cu), and zinc (Zn) level... more The aim of this study was to determine the plasma selenium (Se), copper (Cu), and zinc (Zn) levels and to evaluate their possible association with metabolic syndrome (MetS) components in patients with schizophrenia. The study group consisted of 60 patients with schizophrenia and 60 sex- and age-matched healthy controls. Anthropometric measurements, blood pressure, and biochemical analysis of fasting blood were performed in all subjects. Patients with schizophrenia had significantly higher plasma Cu concentrations compared with controls (0.97 ± 0.31 vs. 0.77 ± 0.32 mg/L, p = 0.001). The plasma Cu concentration showed a positive correlation with plasma glucose and diastolic blood pressure in the patient groups (r s = 0.263, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05 and r s = 0.272, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05, respectively). The plasma Se level correlated positive with MetS score (r s = 0.385, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01), waist circumference (r s = 0.344, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), plasma glucose (r s = 0.319, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), and triglyceride concentrations (r s = 0.462, p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001) in patients with schizophrenia. Plasma Zn did not correlate with any of the MetS components. These results suggest that alterations in plasma Cu and Se levels in medicated patients with schizophrenia could be associated with metabolic risk factors.
Scandinavian Journal of Clinical & Laboratory Investigation, 2014
The purpose of this study was to assess oxidative stress status parameters and their possible ass... more The purpose of this study was to assess oxidative stress status parameters and their possible associations with traditional cardiovascular risk factors in patients with schizophrenia, as well as their potential for patient-control discrimination. Fasting glucose, lipid profile and oxidative stress status parameters were assessed in 30 schizophrenic patients with atypical antipsychotic therapy and 60 control subjects. Malondialdehyde (MDA), pro-oxidant/antioxidant balance (PAB) and total anti-oxidant status (TAS) were significantly higher whereas total sulfhydryl (SH) groups were significantly lower in schizophrenic patients vs. control group. Higher serum PAB values showed an independent association with schizophrenia. The addition of PAB to conventional risk factors improved discrimination between healthy control subjects and patients. Increased oxidative stress and changed lipid profile parameters are associated in schizophrenic patients and may indicate risk for atherosclerosis. The serum PAB level may reflect the levels of oxidative stress in schizophrenia and improve discrimination of patients from controls.
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