The performance of a salt-tolerant pepper (Capsicum annuum L.) accession (A25) utilized as a root... more The performance of a salt-tolerant pepper (Capsicum annuum L.) accession (A25) utilized as a rootstock was assessed in two experiments. In a first field experiment under natural salinity conditions, we observed a larger amount of marketable fruit (+75%) and lower Blossom-end Root incidence (-31%) in commercial pepper cultivar Adige (A) grafted onto A25 (A/A25) when compared with ungrafted plants. In order to understand this behavior a second greenhouse experiment was conducted to determine growth, mineral partitioning, gas exchange and chlorophyll a fluorescence parameters, antioxidant systems and proline content in A and A/A25 plants under salinity conditions (80mM NaCl for 14 days). Salt stress induced significantly stunted growth of A plants (-40.6% of leaf dry weight) compared to the control conditions, while no alterations were observed in A/A25 at the end of the experiment. Accumulation of Na(+) and Cl(-) in leaves and roots was similar in either grafted or ungrafted plants. Despite the activation of protective mechanisms (increment of superoxide dismutase, catalase, ascorbate peroxidase activity and non-photochemical quenching), A plants showed severely reduced photosynthetic CO2 assimilation (-45.6% of AN390) and substantial buildup of malondialdehyde (MDA) by-product, suggesting the inability to counteract salt-triggered damage. In contrast, A/A25 plants, which had a constitutive enhanced root apparatus, were able to maintain the shoot and root growth under salinity conditions by supporting the maintained photosynthetic performance. No increases in catalase and ascorbate peroxidase activities were observed in response to salinity, and MDA levels increased only slightly; indicating that alleviation of oxidative stress did not occur in A/A25 plants. In these plants the increased proline levels could protect enzymatic stability from salt-triggered damage, preserving the photosynthetic performance. The results could indicate that salt stress was vanished by the lack of negative effects on photosynthesis that support the maintained plant growth and increased marketable yield of the grafted plants.
Many chemical and non-chemical strategies have been applied to control weeds in agricultural and ... more Many chemical and non-chemical strategies have been applied to control weeds in agricultural and industrial areas. Knowledge regarding the effects of these methods on roadside vegetation is still poor. A 2-year field experiment was performed along a road located near Livorno (Tuscany, central Italy). Eight plots/strips were identified, of which four were subjected to periodical mechanical mowing and the remaining four were treated with a chemical herbicide based on glyphosate (the producer's recommended rates were used for the selective control of broad-leaved weeds). Our results clearly showed that roadside soil and vegetation are a significant reservoir of anthropogenic activities which have a strong negative effect on several phytosociological, pedochemical and biological parameters. Compared with conventional mechanical mowing, chemical treatment induced (i) a significant increase in organic matter in the upper plot layers (+18 %), and (ii) a marked reduction in weed height throughout the entire period of the experiment. Irrespectively of the kind of treatment, no significance differences were detected in terms of (i) biological quality of soil (the abundance and diversity of arthropod communities did not change), and (ii) plant elemental content (bulk concentrations of analysed trace elements had a good fit within ranges of occurrence in the "reference plant"). The glyphosate partially controlled broad-leaved weeds and this moderate efficacy is dependent upon the season/time of application. In conclusion, the rational and sustainable use of chemical herbicides may be a useful tool for the management of roadside vegetation.
ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were appl... more ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were applied to examine the effect of the “gender” factor on some measurements of diapausing larva and cocoon in Neodryinus typhlocybae (Ashmead) (Hymenoptera Dryinidae). This species was introduced into Italy in 1987 for the biological control of the Nearctic planthopper Metcalfa pruinosa (Say) (Hemiptera Flatidae). Five measurements were taken of each specimen: length (Le) and width (Wi) of the cocoon wall; length (le) and width (wi) of the larval chamber and length of the diapausing larva (dll). Results of MANOVA indicate that all the considered morphometric variables are influenced by the “gender” factor. Results of ANOVAs indicate that length and width of the larval chamber and the length of the diapausing larva are the variables more strictly correlated with the gender.
ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were appl... more ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were applied to examine the effect of the “gender” factor on some measurements of diapausing larva and cocoon in Neodryinus typhlocybae (Ashmead) (Hymenoptera Dryinidae). This species was introduced into Italy in 1987 for the biological control of the Nearctic planthopper Metcalfa pruinosa (Say) (Hemiptera Flatidae). Five measurements were taken of each specimen: length (Le) and width (Wi) of the cocoon wall; length (le) and width (wi) of the larval chamber and length of the diapausing larva (dll). Results of MANOVA indicate that all the considered morphometric variables are influenced by the “gender” factor. Results of ANOVAs indicate that length and width of the larval chamber and the length of the diapausing larva are the variables more strictly correlated with the gender.
Rooted cuttings from two poplar clones (Populus x euramericana, I-214, and Populus deltoides x ma... more Rooted cuttings from two poplar clones (Populus x euramericana, I-214, and Populus deltoides x maximowiczii, Eridano) were exposed for 15 days to diurnal square-wave treatment with ozone (60 nL L-1 for 5 h day-1). Completely fully expanded leaves exposed to ozone showed a reduction in net CO2 assimilation rate as compared to the control leaves during whole exposure period in both the clones. The reduction was related to a strong stomatal closure in clone I-214, but also to an altered mesophyll activity ascribed to limitation of the dark reactions of photosynthetic process. The results obtained in leaves of I-214 subjected to long-term fumigation seem to support the view that the decrease in quantum yield of electron transport may be a mechanism to down-regulate photosynthetic electron transport so that production of ATP and NADPH would be in equilibrium with the decreased demand in the Calvin cycle. In Eridano the CO2 assimilation was reduced because of the exposure and any alteration in stomatal conductance was observed. Thus, chlorophyll fluorescence parameters showed that an inhibition of photosystem II had occurred (reduction in Fv/Fm ratio), while no alterations in quenching parameters were observed upon illumination. The results seem to indicate that an alternative sink for reducing equivalent, other than carbon metabolism is present.
Within the framework of the UN-ECE Convention on Long-Range Trans-boundary Air Pollution, critica... more Within the framework of the UN-ECE Convention on Long-Range Trans-boundary Air Pollution, critical ozone levels for forest trees and crops have been determined. This paper shows the mapping results of short and long-term threshold exceedances in an area of Central Italy. Eighty-thousand hourly mean ozone concentrations were recorded and computed to evaluate the potential distribution of the pollutant in the
A comparative study on functional leaf treats and the diurnal dynamics of photosynthetic processe... more A comparative study on functional leaf treats and the diurnal dynamics of photosynthetic processes was conducted on 2-year-old potted plants of two grape (Vitis vinifera L.) varieties (Aleatico, ALE, and Trebbiano giallo, TRE), exposed under controlled conditions to realistic concentrations of the pollutant gas ozone (80 ppb for 5 h day-1, 8:00-13:00 h, + 40 ppb for 5 h day-1, 13:00-18:00 h). At constitutive levels, the morphological functional traits of TRE improved leaf resistance to gas exchange, suggesting that TRE is characterized by a potential high degree of tolerance to ozone. At the end of the treatment, both varieties showed typical visible injuries on fully expanded leaves and a marked alteration in the diurnal pattern of photosynthetic activity. This was mainly due to a decreased stomatal conductance (-27 and -29% in ALE and TRE, in terms of daily values in comparison to controls) and to a reduced mesophyllic functioning (+33 and +16% of the intercellular carbon dioxide concentration). Although the genotypic variability of grape regulates the response to oxidative stress, similar detoxification processes were activated, such as an increased content of total carotenoids (+64 and +30%, in ALE and TRE), enhanced efficiency of thermal energy dissipation within photosystem II (+32 and +20%) closely correlated with the increased de-epoxidation index (+26 and +22%) and variations in content of some osmolytes. In summary, we can conclude that: the daily photosynthetic performance of grapevine leaves was affected by a realistic exposure to ozone. In addition, the gas exchange and chlorophyll a fluorescence measurements revealed a different quali-quantitative response in the two varieties. The genotypic variability of V. vinifera and the functional leaf traits would seem to regulate the acclimatory response to oxidative stress and the degree of tolerance to ozone. Similar photoprotective mechanisms were activated in the two varieties, though to a different extent.
Ozone affects volatile organic compounds that protect plants from biotic and abiotic stress. In v... more Ozone affects volatile organic compounds that protect plants from biotic and abiotic stress. In vitro Melissa officinalis shoots were exposed to ozone (200 ppb, 3 h) in controlled environmental conditions: leaf pigments, membrane integrity and headspace composition were assayed during fumigation and after the recovery period (3 h from the beginning of the exposure, FBE). At the end of the exposure, no injury was observed in untreated and treated shoots, although an evident increase in lipid peroxidation was reported (+38.5 and +37.2% of TBARS levels in comparison with controls, respectively after 1 and 3 h FBE). The levels of total carotenoids significantly rose as a normal response mechanism to oxidative stress. SPME-GS-MS analysis showed that, as a consequence of the fumigation, the trends in non-terpenoid compounds increased after 1 and 3 h FBE. This suggests that the concentration and the duration of the treatment were enough to cause a breakdown of cells (as evidenced by increa...
The performance of a salt-tolerant pepper (Capsicum annuum L.) accession (A25) utilized as a root... more The performance of a salt-tolerant pepper (Capsicum annuum L.) accession (A25) utilized as a rootstock was assessed in two experiments. In a first field experiment under natural salinity conditions, we observed a larger amount of marketable fruit (+75%) and lower Blossom-end Root incidence (-31%) in commercial pepper cultivar Adige (A) grafted onto A25 (A/A25) when compared with ungrafted plants. In order to understand this behavior a second greenhouse experiment was conducted to determine growth, mineral partitioning, gas exchange and chlorophyll a fluorescence parameters, antioxidant systems and proline content in A and A/A25 plants under salinity conditions (80mM NaCl for 14 days). Salt stress induced significantly stunted growth of A plants (-40.6% of leaf dry weight) compared to the control conditions, while no alterations were observed in A/A25 at the end of the experiment. Accumulation of Na(+) and Cl(-) in leaves and roots was similar in either grafted or ungrafted plants. Despite the activation of protective mechanisms (increment of superoxide dismutase, catalase, ascorbate peroxidase activity and non-photochemical quenching), A plants showed severely reduced photosynthetic CO2 assimilation (-45.6% of AN390) and substantial buildup of malondialdehyde (MDA) by-product, suggesting the inability to counteract salt-triggered damage. In contrast, A/A25 plants, which had a constitutive enhanced root apparatus, were able to maintain the shoot and root growth under salinity conditions by supporting the maintained photosynthetic performance. No increases in catalase and ascorbate peroxidase activities were observed in response to salinity, and MDA levels increased only slightly; indicating that alleviation of oxidative stress did not occur in A/A25 plants. In these plants the increased proline levels could protect enzymatic stability from salt-triggered damage, preserving the photosynthetic performance. The results could indicate that salt stress was vanished by the lack of negative effects on photosynthesis that support the maintained plant growth and increased marketable yield of the grafted plants.
Many chemical and non-chemical strategies have been applied to control weeds in agricultural and ... more Many chemical and non-chemical strategies have been applied to control weeds in agricultural and industrial areas. Knowledge regarding the effects of these methods on roadside vegetation is still poor. A 2-year field experiment was performed along a road located near Livorno (Tuscany, central Italy). Eight plots/strips were identified, of which four were subjected to periodical mechanical mowing and the remaining four were treated with a chemical herbicide based on glyphosate (the producer's recommended rates were used for the selective control of broad-leaved weeds). Our results clearly showed that roadside soil and vegetation are a significant reservoir of anthropogenic activities which have a strong negative effect on several phytosociological, pedochemical and biological parameters. Compared with conventional mechanical mowing, chemical treatment induced (i) a significant increase in organic matter in the upper plot layers (+18 %), and (ii) a marked reduction in weed height throughout the entire period of the experiment. Irrespectively of the kind of treatment, no significance differences were detected in terms of (i) biological quality of soil (the abundance and diversity of arthropod communities did not change), and (ii) plant elemental content (bulk concentrations of analysed trace elements had a good fit within ranges of occurrence in the "reference plant"). The glyphosate partially controlled broad-leaved weeds and this moderate efficacy is dependent upon the season/time of application. In conclusion, the rational and sustainable use of chemical herbicides may be a useful tool for the management of roadside vegetation.
ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were appl... more ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were applied to examine the effect of the “gender” factor on some measurements of diapausing larva and cocoon in Neodryinus typhlocybae (Ashmead) (Hymenoptera Dryinidae). This species was introduced into Italy in 1987 for the biological control of the Nearctic planthopper Metcalfa pruinosa (Say) (Hemiptera Flatidae). Five measurements were taken of each specimen: length (Le) and width (Wi) of the cocoon wall; length (le) and width (wi) of the larval chamber and length of the diapausing larva (dll). Results of MANOVA indicate that all the considered morphometric variables are influenced by the “gender” factor. Results of ANOVAs indicate that length and width of the larval chamber and the length of the diapausing larva are the variables more strictly correlated with the gender.
ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were appl... more ABSTRACT Univariate and multivariate one-factor analyses of variance (ANOVA and MANOVA) were applied to examine the effect of the “gender” factor on some measurements of diapausing larva and cocoon in Neodryinus typhlocybae (Ashmead) (Hymenoptera Dryinidae). This species was introduced into Italy in 1987 for the biological control of the Nearctic planthopper Metcalfa pruinosa (Say) (Hemiptera Flatidae). Five measurements were taken of each specimen: length (Le) and width (Wi) of the cocoon wall; length (le) and width (wi) of the larval chamber and length of the diapausing larva (dll). Results of MANOVA indicate that all the considered morphometric variables are influenced by the “gender” factor. Results of ANOVAs indicate that length and width of the larval chamber and the length of the diapausing larva are the variables more strictly correlated with the gender.
Rooted cuttings from two poplar clones (Populus x euramericana, I-214, and Populus deltoides x ma... more Rooted cuttings from two poplar clones (Populus x euramericana, I-214, and Populus deltoides x maximowiczii, Eridano) were exposed for 15 days to diurnal square-wave treatment with ozone (60 nL L-1 for 5 h day-1). Completely fully expanded leaves exposed to ozone showed a reduction in net CO2 assimilation rate as compared to the control leaves during whole exposure period in both the clones. The reduction was related to a strong stomatal closure in clone I-214, but also to an altered mesophyll activity ascribed to limitation of the dark reactions of photosynthetic process. The results obtained in leaves of I-214 subjected to long-term fumigation seem to support the view that the decrease in quantum yield of electron transport may be a mechanism to down-regulate photosynthetic electron transport so that production of ATP and NADPH would be in equilibrium with the decreased demand in the Calvin cycle. In Eridano the CO2 assimilation was reduced because of the exposure and any alteration in stomatal conductance was observed. Thus, chlorophyll fluorescence parameters showed that an inhibition of photosystem II had occurred (reduction in Fv/Fm ratio), while no alterations in quenching parameters were observed upon illumination. The results seem to indicate that an alternative sink for reducing equivalent, other than carbon metabolism is present.
Within the framework of the UN-ECE Convention on Long-Range Trans-boundary Air Pollution, critica... more Within the framework of the UN-ECE Convention on Long-Range Trans-boundary Air Pollution, critical ozone levels for forest trees and crops have been determined. This paper shows the mapping results of short and long-term threshold exceedances in an area of Central Italy. Eighty-thousand hourly mean ozone concentrations were recorded and computed to evaluate the potential distribution of the pollutant in the
A comparative study on functional leaf treats and the diurnal dynamics of photosynthetic processe... more A comparative study on functional leaf treats and the diurnal dynamics of photosynthetic processes was conducted on 2-year-old potted plants of two grape (Vitis vinifera L.) varieties (Aleatico, ALE, and Trebbiano giallo, TRE), exposed under controlled conditions to realistic concentrations of the pollutant gas ozone (80 ppb for 5 h day-1, 8:00-13:00 h, + 40 ppb for 5 h day-1, 13:00-18:00 h). At constitutive levels, the morphological functional traits of TRE improved leaf resistance to gas exchange, suggesting that TRE is characterized by a potential high degree of tolerance to ozone. At the end of the treatment, both varieties showed typical visible injuries on fully expanded leaves and a marked alteration in the diurnal pattern of photosynthetic activity. This was mainly due to a decreased stomatal conductance (-27 and -29% in ALE and TRE, in terms of daily values in comparison to controls) and to a reduced mesophyllic functioning (+33 and +16% of the intercellular carbon dioxide concentration). Although the genotypic variability of grape regulates the response to oxidative stress, similar detoxification processes were activated, such as an increased content of total carotenoids (+64 and +30%, in ALE and TRE), enhanced efficiency of thermal energy dissipation within photosystem II (+32 and +20%) closely correlated with the increased de-epoxidation index (+26 and +22%) and variations in content of some osmolytes. In summary, we can conclude that: the daily photosynthetic performance of grapevine leaves was affected by a realistic exposure to ozone. In addition, the gas exchange and chlorophyll a fluorescence measurements revealed a different quali-quantitative response in the two varieties. The genotypic variability of V. vinifera and the functional leaf traits would seem to regulate the acclimatory response to oxidative stress and the degree of tolerance to ozone. Similar photoprotective mechanisms were activated in the two varieties, though to a different extent.
Ozone affects volatile organic compounds that protect plants from biotic and abiotic stress. In v... more Ozone affects volatile organic compounds that protect plants from biotic and abiotic stress. In vitro Melissa officinalis shoots were exposed to ozone (200 ppb, 3 h) in controlled environmental conditions: leaf pigments, membrane integrity and headspace composition were assayed during fumigation and after the recovery period (3 h from the beginning of the exposure, FBE). At the end of the exposure, no injury was observed in untreated and treated shoots, although an evident increase in lipid peroxidation was reported (+38.5 and +37.2% of TBARS levels in comparison with controls, respectively after 1 and 3 h FBE). The levels of total carotenoids significantly rose as a normal response mechanism to oxidative stress. SPME-GS-MS analysis showed that, as a consequence of the fumigation, the trends in non-terpenoid compounds increased after 1 and 3 h FBE. This suggests that the concentration and the duration of the treatment were enough to cause a breakdown of cells (as evidenced by increa...
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