The gaseus molecule ethylene (C2H4), industrially produced worldwide, small in size and simple in structure, is also a plant hormone usually associated to fruit ripening. Its effects are known since the ancient Egypt in agriculture, yet the complexity of its mode of action and the broad spectrum of effects (and potential uses) in plant physiology is still challenging scientists. In the last few decades, the biochemical pathway to ethylene production has been described, and it was discovered how plants produce gas receptors able to perceive it, ethylene-responsive transcription factors were indentified and new molecular mediated effects for ethylene are described, ranging from water stress (regardless is flooding or drought) development and senescence, reproduction and, of course, ripening. Last but not least, plant-pathogen interactions. Thus ethylene is involved in the developmental life of plants, in biotic and abiotic stress, in reproduction. There is no stage in plant life that is not affected by ethylene and modulated by a complex and fascinating molecular machinery.
The plant hormone ethylene plays vital roles in plant development, including pollen tube (PT) gro... more The plant hormone ethylene plays vital roles in plant development, including pollen tube (PT) growth. Many studies have used the ethylene precursor, 1‐aminocyclopropane‐1‐carboxylic acid (ACC), as a tool to trigger ethylene signaling. Several studies have suggested that ACC can act as a signal molecule independently of ethylene, inducing responses that are distinct from those induced by ethylene. In this study, we confirmed that ethylene receptor function is essential for promoting PT growth in tomato, but interestingly, we discovered that ACC itself can act as a signal that also promotes PT growth. Exogenous ACC stimulated PT growth even when ethylene perception was inhibited either chemically by treating with 1‐methylcyclopropene (1‐MCP) or genetically by using the ethylene‐insensitive Never Ripe (NR) mutant. Treatment with aminoethoxyvinylglycine, which reduces endogenous ACC levels, led to a reduction of PT growth, even in the NR mutants. Furthermore, GUS activity driven by an E...
The invention includes transformed plants having at least one cell transformed with a modified ET... more The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic
Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth... more Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In Arabidopsis thaliana, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that the ltp1 knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), while LTP1 overexpression confers insensitivity. Analys...
This is an Open Access article distributed under the terms of the Creative Commons Attribution Li... more This is an Open Access article distributed under the terms of the Creative Commons Attribution License
The gaseus molecule ethylene (C2H4), industrially produced worldwide, small in size and simple in structure, is also a plant hormone usually associated to fruit ripening. Its effects are known since the ancient Egypt in agriculture, yet the complexity of its mode of action and the broad spectrum of effects (and potential uses) in plant physiology is still challenging scientists. In the last few decades, the biochemical pathway to ethylene production has been described, and it was discovered how plants produce gas receptors able to perceive it, ethylene-responsive transcription factors were indentified and new molecular mediated effects for ethylene are described, ranging from water stress (regardless is flooding or drought) development and senescence, reproduction and, of course, ripening. Last but not least, plant-pathogen interactions. Thus ethylene is involved in the developmental life of plants, in biotic and abiotic stress, in reproduction. There is no stage in plant life that is not affected by ethylene and modulated by a complex and fascinating molecular machinery.
The plant hormone ethylene plays vital roles in plant development, including pollen tube (PT) gro... more The plant hormone ethylene plays vital roles in plant development, including pollen tube (PT) growth. Many studies have used the ethylene precursor, 1‐aminocyclopropane‐1‐carboxylic acid (ACC), as a tool to trigger ethylene signaling. Several studies have suggested that ACC can act as a signal molecule independently of ethylene, inducing responses that are distinct from those induced by ethylene. In this study, we confirmed that ethylene receptor function is essential for promoting PT growth in tomato, but interestingly, we discovered that ACC itself can act as a signal that also promotes PT growth. Exogenous ACC stimulated PT growth even when ethylene perception was inhibited either chemically by treating with 1‐methylcyclopropene (1‐MCP) or genetically by using the ethylene‐insensitive Never Ripe (NR) mutant. Treatment with aminoethoxyvinylglycine, which reduces endogenous ACC levels, led to a reduction of PT growth, even in the NR mutants. Furthermore, GUS activity driven by an E...
The invention includes transformed plants having at least one cell transformed with a modified ET... more The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic
Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth... more Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. In Arabidopsis thaliana, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that the ltp1 knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), while LTP1 overexpression confers insensitivity. Analys...
This is an Open Access article distributed under the terms of the Creative Commons Attribution Li... more This is an Open Access article distributed under the terms of the Creative Commons Attribution License
In seed plants, 1-amino-cyclopropane-1-carboxylic acid (ACC) is the well-known precursor of the p... more In seed plants, 1-amino-cyclopropane-1-carboxylic acid (ACC) is the well-known precursor of the plant hormone ethylene. In nonseed plants, the current view is that ACC is produced but is inefficiently converted to ethylene. Distinct responses to ACC that are uncoupled from ethylene biosynthesis have been discovered in diverse aspects of growth and development in liverworts and angiosperms, indicating that ACC itself can function as a signal. Evolutionarily, ACC may have served as a signal before acquiring its role as the ethylene precursor in seed plants. These findings pave the way for unraveling a potentially conserved ACC signaling pathway in plants and have ramifications for the use of ACC as a substitute for ethylene treatment in seed plants.
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The gaseus molecule ethylene (C2H4), industrially produced worldwide, small in size and simple in structure, is also a plant hormone usually associated to fruit ripening. Its effects are known since the ancient Egypt in agriculture, yet the complexity of its mode of action and the broad spectrum of effects (and potential uses) in plant physiology is still challenging scientists. In the last few decades, the biochemical pathway to ethylene production has been described, and it was discovered how plants produce gas receptors able to perceive it, ethylene-responsive transcription factors were indentified and new molecular mediated effects for ethylene are described, ranging from water stress (regardless is flooding or drought) development and senescence, reproduction and, of course, ripening. Last but not least, plant-pathogen interactions. Thus ethylene is involved in the developmental life of plants, in biotic and abiotic stress, in reproduction. There is no stage in plant life that is not affected by ethylene and modulated by a complex and fascinating molecular machinery.
Papers by Caren Chang
The gaseus molecule ethylene (C2H4), industrially produced worldwide, small in size and simple in structure, is also a plant hormone usually associated to fruit ripening. Its effects are known since the ancient Egypt in agriculture, yet the complexity of its mode of action and the broad spectrum of effects (and potential uses) in plant physiology is still challenging scientists. In the last few decades, the biochemical pathway to ethylene production has been described, and it was discovered how plants produce gas receptors able to perceive it, ethylene-responsive transcription factors were indentified and new molecular mediated effects for ethylene are described, ranging from water stress (regardless is flooding or drought) development and senescence, reproduction and, of course, ripening. Last but not least, plant-pathogen interactions. Thus ethylene is involved in the developmental life of plants, in biotic and abiotic stress, in reproduction. There is no stage in plant life that is not affected by ethylene and modulated by a complex and fascinating molecular machinery.