... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA ... more ... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA according to Mrva and Mares [4] and ... 1). LMA-prone breeding lines and cultivars, Kennedy, Suneca, BD159, Cranbrook and Super Seri, which contained Rht1 or Rht2 had low α-amylase ...
Propionibacterium is a facultative anaerobe associated with the rumen epithelium, the presence of... more Propionibacterium is a facultative anaerobe associated with the rumen epithelium, the presence of which may influence the anaerobic environment through oxygen scavenging, as well as providing a source of propionate. Factors such as bacteriophages that influence Propionibacterium populations may therefore be important regulators of rumen function. This study describes the isolation and identification of a ruminal Propionibacterium bacteriophage. Sheep rumen fluid was screened for Propionibacterium species and 3 isolates were identified and characterised. One isolate, PA1, was used as an indicator strain to screen for the presence of Propionibacterium-specific virulent bacteriophages. A virulent bacteriophage, PB2, was isolated from clear plaques on a lawn of PA1 cells and was shown by transmission electron microscopy to be a siphovirus-like particle comprising an icosahedral head 50 nm in diameter and a tail 140 nm in length. The bacteriophage was visibly attached to and within PA1 cells, and was shown to infect all 3 ruminal isolates of Propionibacterium and 4 of 6 clinical isolates of P. acnes. Restriction mapping of bacteriophage PB2 demonstrated a 30.8 kb genome.
Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease ... more Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease problem to the barley industry worldwide that has not, to date, been detected in Australia. This paper describes the mapping of stripe rust resistance genes in Tallon/Kaputar (TK) and Arapiles/Franklin (AF) populations. The paper also reports on the usefulness of markers associated with alternative sources of resistance previously identified in the varieties Orca and Shyri in the USA. Stripe rust screening was conducted at the adult plant stage in Toluca, Mexico, for 2 years. Two major quantitative trait loci (QTLs) were found on chromosomes 2H and 5H in both populations. One region on chromosome 5H was highly significantly associated with resistance to stripe rust (R2 = 68% and 34% in TK and AF, respectively). The QTL on chromosome 2H accounted for 36% of the variation in TK and 10% of the variation in the AF population. These associations were consistent over both years. Further work w...
... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA ... more ... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA according to Mrva and Mares [4] and ... 1). LMA-prone breeding lines and cultivars, Kennedy, Suneca, BD159, Cranbrook and Super Seri, which contained Rht1 or Rht2 had low α-amylase ...
... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA ... more ... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA according to Mrva and Mares [4] and ... 1). LMA-prone breeding lines and cultivars, Kennedy, Suneca, BD159, Cranbrook and Super Seri, which contained Rht1 or Rht2 had low α-amylase ...
Propionibacterium is a facultative anaerobe associated with the rumen epithelium, the presence of... more Propionibacterium is a facultative anaerobe associated with the rumen epithelium, the presence of which may influence the anaerobic environment through oxygen scavenging, as well as providing a source of propionate. Factors such as bacteriophages that influence Propionibacterium populations may therefore be important regulators of rumen function. This study describes the isolation and identification of a ruminal Propionibacterium bacteriophage. Sheep rumen fluid was screened for Propionibacterium species and 3 isolates were identified and characterised. One isolate, PA1, was used as an indicator strain to screen for the presence of Propionibacterium-specific virulent bacteriophages. A virulent bacteriophage, PB2, was isolated from clear plaques on a lawn of PA1 cells and was shown by transmission electron microscopy to be a siphovirus-like particle comprising an icosahedral head 50 nm in diameter and a tail 140 nm in length. The bacteriophage was visibly attached to and within PA1 cells, and was shown to infect all 3 ruminal isolates of Propionibacterium and 4 of 6 clinical isolates of P. acnes. Restriction mapping of bacteriophage PB2 demonstrated a 30.8 kb genome.
Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease ... more Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease problem to the barley industry worldwide that has not, to date, been detected in Australia. This paper describes the mapping of stripe rust resistance genes in Tallon/Kaputar (TK) and Arapiles/Franklin (AF) populations. The paper also reports on the usefulness of markers associated with alternative sources of resistance previously identified in the varieties Orca and Shyri in the USA. Stripe rust screening was conducted at the adult plant stage in Toluca, Mexico, for 2 years. Two major quantitative trait loci (QTLs) were found on chromosomes 2H and 5H in both populations. One region on chromosome 5H was highly significantly associated with resistance to stripe rust (R2 = 68% and 34% in TK and AF, respectively). The QTL on chromosome 2H accounted for 36% of the variation in TK and 10% of the variation in the AF population. These associations were consistent over both years. Further work w...
... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA ... more ... α-Amylase activity in the distal halves of grains was determined with high pI-specific ELISA according to Mrva and Mares [4] and ... 1). LMA-prone breeding lines and cultivars, Kennedy, Suneca, BD159, Cranbrook and Super Seri, which contained Rht1 or Rht2 had low α-amylase ...
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