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    D. Mousain

    International audienc
    National audienc
    The phosphatase activity of cell walls isolated by treating the intact roots with Triton X 100 is very steady. It is similar to that of living roots and that of cell walls isolated by centrifugation after the grinding of the tissues.
    Research Interests:
    As chitin is a characteristic component of most fungi, and not of plants, its assay can give a quantitative estimate of the fungal biomass in plant/fungal relationships, such as pathogenesis and mycorrhizal associations. Here follow two... more
    As chitin is a characteristic component of most fungi, and not of plants, its assay can give a quantitative estimate of the fungal biomass in plant/fungal relationships, such as pathogenesis and mycorrhizal associations. Here follow two investigations of the usefulness of this idea.
    summaryThe purpose of this study was to determine how ectomycorrhizal infection and phosphorus nutrition affect biomass, photosynthesis and root respiration in the host plant. Maritime pine (Pinus pinaster Soland. in Ait.) seedlings grown... more
    summaryThe purpose of this study was to determine how ectomycorrhizal infection and phosphorus nutrition affect biomass, photosynthesis and root respiration in the host plant. Maritime pine (Pinus pinaster Soland. in Ait.) seedlings grown in containers filled with perlite–vermiculite were inoculated with the ectomycorrhizal fungus Hebeloma cylindrosporum (strain D3.25.9) and given 0 or 0.5 mM phosphate in the nutrient solution. Hebeloma cylindrosporum infection increased net photosynthesis and root respiration rates compared with those of non‐mycorrhizal plants, but there was an accompanying 35 % depression in growth. The addition of phosphorus to non‐mycorrhizal plants induced a rise in tissue phosphorus content which made them similar m that respect to mycorrhizal plants but did not result in increased photosynthesis. The nitrogen content of mycorrhizal plants was, moreover, lower than that of the control group. The data recorded were consistent with the photosynthate source‐sink ...
    Selection of ectomycorrhizal strains for application in forestry is mostly based on the evaluation of symbiotic performance in small-scale experiments. Two Suillus collinitus strains isolated from a Mediterranean and an alpine area were... more
    Selection of ectomycorrhizal strains for application in forestry is mostly based on the evaluation of symbiotic performance in small-scale experiments. Two Suillus collinitus strains isolated from a Mediterranean and an alpine area were inoculated onto two pine tree species (Pinus pinea and P. nigra ssp. laricio var. corsicana) typical of these two environments. The early events during contact between the
    Nitrate uptake rates were determined for nonmycorrhizal and mycorrhizal maritime pine (Pinus pinaster) associated with the ectomycorrhizal basidiomycete Hebeloma cylindrosporum and for this fungal species grown in vitro. Different [NO3−]... more
    Nitrate uptake rates were determined for nonmycorrhizal and mycorrhizal maritime pine (Pinus pinaster) associated with the ectomycorrhizal basidiomycete Hebeloma cylindrosporum and for this fungal species grown in vitro. Different [NO3−] (0–1.3 and 10 mM) were supplied to nonmycorrhizal or mycorrhizal P. pinaster plants grown axenically in test tubes for 21 weeks. Uptake of NO3− by the plants was determined by measuring NO3− depletion from the medium that was added to the culture tubes without root pertubation. For all applied [NO3−] the uptake rates for NO3− in mycorrhizal plants were increased about twofold compared with nonmycorrhizal plants. The same range of [NO3−] was supplied to the fungus in pure culture, and uptake rates were calculated either from the measurement of NO3− depletion from the medium or by 15N accumulation in the mycelium. For the same external nitrate concentrations the results obtained with these two methods were not significantly different. The amount of fu...
    ResumeLes roles respectifs des partenaires de la symbiose ectomycorhizienne dans les metabolismes carbone, phosphate et azote des plantes mycorhizees sont etudies. Pour les echanges de carbone, la plante-hote est essentiellement la source... more
    ResumeLes roles respectifs des partenaires de la symbiose ectomycorhizienne dans les metabolismes carbone, phosphate et azote des plantes mycorhizees sont etudies. Pour les echanges de carbone, la plante-hote est essentiellement la source et le mycosymbiote le puits. Cependant, les donnees quantitatives montrent que les mycorhizes peuvent ne pas etre le puits ultime des photosynthetats de cette plante. Pour les echanges de phosphore et d'azote, le phytosymbiote est le puits, le symbiote fongique la source.Les principales connaissances acquises au Laboratoire sur les metabolismes phosphate et azote du Pin maritime et des champignons Basidiomycetes ectomycorhiziens cultives separement sont presentees. A l'aide de ces donnees, le role possible du mycosymbiote dans la nutrition phosphatee et azotee de la plante-hote est propose.
    L'etude a concerne 98 echantillons de mycorhizes, de basidiomes et de cultures pures de Pisolithus spp. provenant d'une plantation de Chene-liege de la Maâmora orientale. Le produit d'amplification de l'ITS des Pisolithus... more
    L'etude a concerne 98 echantillons de mycorhizes, de basidiomes et de cultures pures de Pisolithus spp. provenant d'une plantation de Chene-liege de la Maâmora orientale. Le produit d'amplification de l'ITS des Pisolithus spp. a montre la presence d'une bande electrophoretique de poids moleculaire compris entre 600 et 700 pb. L'analyse des profils de restriction, obtenus par les enzymes Hinf I, Msp 1 et Hae III, a permis de differencier 2 types genetiques au sein des Pisolithus spp. Les 2 genotypes presentent une distribution spatiale particuliere dans une plantation de chenes-lieges de la Maâmora orientale. Cependant, toutes les mycorhizes de Pisolithus spp. recoltees dans cette plantation appartenaient au genotype 1 (P. arrhizus s.s.), aucune au genotype 2.
    National audienc
    Summary Assimilation pathways of mineral nitrogen and ion balances of the two partners of ectomycorrhizal symbiosis (fungi and woody plants) are reviewed. Data are presented about the partners both in pure culture and in mycorrhizal... more
    Summary Assimilation pathways of mineral nitrogen and ion balances of the two partners of ectomycorrhizal symbiosis (fungi and woody plants) are reviewed. Data are presented about the partners both in pure culture and in mycorrhizal association. The two forms of mineral nitrogen, ammonium and nitrate, differ in their mobility in the soil, their transport into the cells, their uptake rates
    The mycorrhizal relationships between pines and two edible species of Lactarius sect. Dapetes were investigated by optimizing the experimental conditions of mycelial growth and of mycorrhizal colonization of pine seedlings. In vitro... more
    The mycorrhizal relationships between pines and two edible species of Lactarius sect. Dapetes were investigated by optimizing the experimental conditions of mycelial growth and of mycorrhizal colonization of pine seedlings. In vitro mycelial growth of Lactarius deliciosus and L. sanguifluus was improved on a buffered medium containing glucose, amino acids, and vitamins. Two methods of mycorrhization of pines with Lactarius deliciosus were tested. The mycorrhizal colonization was rapid and intense under non-aseptic conditions with a low nutrient supply and without exogenous glucose. A positive influence of mycorrhizal colonization on Pinus sylvestris growth was subsequently observed. Under axenic conditions and with a high nutrient supply, mycorrhization was stimulated at 10 g/L of exogenous glucose, irrespective of the phosphorus concentration. At high phosphorus level (1 mM) and 0.1, 1.0, or 10.0 g/L glucose, growth of Pinus sylvestris was reduced by inoculation. Stability and development of Pinus spp./Lactarius deliciosus symbioses were assayed in a climatic chamber using containers filled with a synthetic substrate. Over a 2-year culture period, the root systems of the pine seedlings were heavily colonized by Lactarius deliciosus. One year following inoculation, Lactarius deliciosus fruit-body primordia appeared associated with Pinus sylvestris seedlings. Six months later, two mature basidiomata were obtained. This is the first report of soilless fruit-body formation of this edible mushroom.