Objective:To observe the protective effect of anti-LINGO-1 antibody on the motor and sensory axon... more Objective:To observe the protective effect of anti-LINGO-1 antibody on the motor and sensory axons after mice spinal cord injury(SCI).Methods:Anti-LINGO-1 antibody was administrated by intraperitoneal injection to the mice after a precise 1.1 mm depth dorsal laceration on the 9th thoracic spinal segment was generated by LISA(Louisville injury systems apparatus).Immunohistochemistry or immunofluorescence was used to detect the changes of biotin dextran amine(BDA) labeled descending axons and cholera toxin B subunit(CTB) labeled ascending axons.Results:In anti-LINGO-1 antibody treated mice,a few BDA positive fibers grew into or beyond the lesion gap,and then,extended to the caudal white matter.The longest one reached the site of 1.5 mm caudal to the lesion epicenter.At the same time,more CTB positive sensory fibers were found at the edge of lesion gap than the control group,but no CTB positive fibers extended into or beyond the lesion gap.In IgG treated control mice,neither BDA nor CT...
This experiment aimed to evaluate meat quality, fatty acid profile in back-fat, and fecal microbi... more This experiment aimed to evaluate meat quality, fatty acid profile in back-fat, and fecal microbiota of growing-finishing pigs fed with liquid enzymatically digested food waste. Fifty-six crossbred pigs (approximately 32.99 kg body weight) were assigned to one of two treatments with seven replicate pens and four pigs per pen. Pigs were fed with control (corn-soybean meal diets) or food waste from d 0 to 53, while all pigs were fed with the control diet from d 53 to 79. The 16S rRNA sequencing was used to analyze microbiota of feces collected on d 0, 28, 53, and 79. Meat quality and carcass characteristics were measured in one pig per pen at the end of the experiment. Pigs fed with food waste contained more (p < 0.05) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in back-fat. Feeding food waste increased (p < 0.05) the relative abundances of Lachnospiraceae and Ruminococcaceae, but decreased (p < 0.05) the relative abundances of Streptococcaceae and Clostridiace...
Increasing emphasis on controlling the uses of chemical fertilizers requires identifying safe Org... more Increasing emphasis on controlling the uses of chemical fertilizers requires identifying safe Organic Soil Amendments (OSA) to use as alternatives. Converting organic waste, such as foodwaste into an OSA can be an option. Such approaches are also an attempt to make beneficial use of the enormous amount of foodwaste generated globally. In this study we conducted a pathogen challenge to determine the inac-tivation of three foodborne pathogens in an OSA derived from a complex foodwaste stream. Further, the physiochemical characteristics of the OSA were assessed at pilot-scale experiments. The inactivation of three most common foodborne pathogens (Escherichia coli O157:H7, Salmonella enterica subspecies enterica sv Typhimurium LT2, and Listeria monocytogenes) was determined using bench-scale tests, simulating the process adopted at a pilot-scale facility. The pilot-scale facility uses three processes (enzyme digestion (55e57 C), pasteurization (75e77 C), and acidification treatments) for producing the OSA. In addition, the yields and nutrient characteristics of the OSA were analyzed using 16 pilot-scale batch tests. The results showed that the process adopted in this study for converting foodwaste to the OSA produced a soil amendment with non-detectable levels of E. coli O157:H7, Salmonella LT2, and L. monocytogenes. The yield of the OSA was 84e96% of the initial foodwaste inputs, and organic matter and C: N ratio of the OSA were 20e25% and 12:1, respectively. We anticipate that the results presented here will help in enhancing agricultural sustainability.
Objective:To observe the protective effect of anti-LINGO-1 antibody on the motor and sensory axon... more Objective:To observe the protective effect of anti-LINGO-1 antibody on the motor and sensory axons after mice spinal cord injury(SCI).Methods:Anti-LINGO-1 antibody was administrated by intraperitoneal injection to the mice after a precise 1.1 mm depth dorsal laceration on the 9th thoracic spinal segment was generated by LISA(Louisville injury systems apparatus).Immunohistochemistry or immunofluorescence was used to detect the changes of biotin dextran amine(BDA) labeled descending axons and cholera toxin B subunit(CTB) labeled ascending axons.Results:In anti-LINGO-1 antibody treated mice,a few BDA positive fibers grew into or beyond the lesion gap,and then,extended to the caudal white matter.The longest one reached the site of 1.5 mm caudal to the lesion epicenter.At the same time,more CTB positive sensory fibers were found at the edge of lesion gap than the control group,but no CTB positive fibers extended into or beyond the lesion gap.In IgG treated control mice,neither BDA nor CT...
This experiment aimed to evaluate meat quality, fatty acid profile in back-fat, and fecal microbi... more This experiment aimed to evaluate meat quality, fatty acid profile in back-fat, and fecal microbiota of growing-finishing pigs fed with liquid enzymatically digested food waste. Fifty-six crossbred pigs (approximately 32.99 kg body weight) were assigned to one of two treatments with seven replicate pens and four pigs per pen. Pigs were fed with control (corn-soybean meal diets) or food waste from d 0 to 53, while all pigs were fed with the control diet from d 53 to 79. The 16S rRNA sequencing was used to analyze microbiota of feces collected on d 0, 28, 53, and 79. Meat quality and carcass characteristics were measured in one pig per pen at the end of the experiment. Pigs fed with food waste contained more (p < 0.05) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in back-fat. Feeding food waste increased (p < 0.05) the relative abundances of Lachnospiraceae and Ruminococcaceae, but decreased (p < 0.05) the relative abundances of Streptococcaceae and Clostridiace...
Increasing emphasis on controlling the uses of chemical fertilizers requires identifying safe Org... more Increasing emphasis on controlling the uses of chemical fertilizers requires identifying safe Organic Soil Amendments (OSA) to use as alternatives. Converting organic waste, such as foodwaste into an OSA can be an option. Such approaches are also an attempt to make beneficial use of the enormous amount of foodwaste generated globally. In this study we conducted a pathogen challenge to determine the inac-tivation of three foodborne pathogens in an OSA derived from a complex foodwaste stream. Further, the physiochemical characteristics of the OSA were assessed at pilot-scale experiments. The inactivation of three most common foodborne pathogens (Escherichia coli O157:H7, Salmonella enterica subspecies enterica sv Typhimurium LT2, and Listeria monocytogenes) was determined using bench-scale tests, simulating the process adopted at a pilot-scale facility. The pilot-scale facility uses three processes (enzyme digestion (55e57 C), pasteurization (75e77 C), and acidification treatments) for producing the OSA. In addition, the yields and nutrient characteristics of the OSA were analyzed using 16 pilot-scale batch tests. The results showed that the process adopted in this study for converting foodwaste to the OSA produced a soil amendment with non-detectable levels of E. coli O157:H7, Salmonella LT2, and L. monocytogenes. The yield of the OSA was 84e96% of the initial foodwaste inputs, and organic matter and C: N ratio of the OSA were 20e25% and 12:1, respectively. We anticipate that the results presented here will help in enhancing agricultural sustainability.
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