K ABSTRACT: The purpose of this study was to define the incidence, dynamics, and profiles of anti... more K ABSTRACT: The purpose of this study was to define the incidence, dynamics, and profiles of anti–human leukocyte antigen antibodies (HLA-Abs) produced after kidney transplantation and their impact on graft outcome. A total of 72 first cadaver donor kidney recipients were prospectively monitored for the development of HLA-Abs using beadbased flow-cytometry assays (One Lambda FlowPRA tests). Sixteen recipients (22.2%) developed HLA-Abs after transplantation (class I, n 7; class I II, n 6; class II, n 3), in most cases (81.25%) within the first 2 weeks posttransplantation. A strong association between alloantibody presence and delayed graft function (Chi-square 7.659, p 0.01), acute rejection (Chi-square 14.504, p 0.001), chronic rejection (Chi-square 12.84, p 0.001), and graft loss (Chi-square 20.283, p 0.001) was found. Patients with higher alloantibody titers experienced acute rejections and even early graft loss, compared with those r
The aerobiological study of the Ambrosia pollen in Sofia, Bulgaria began in 1991. The quantitativ... more The aerobiological study of the Ambrosia pollen in Sofia, Bulgaria began in 1991. The quantitative dynamics of Ambrosia pollen grains during the last seven years was determined by Chebishev's method of parabolic interpolation with orthogonal polynoms. Thesensitization to ragweed pollen was investigated in random groups of patients with clinical manifestation of hay fever.
Chronic allograft nephropathy (CAN) is one of the main causes of graft loss in renal transplantat... more Chronic allograft nephropathy (CAN) is one of the main causes of graft loss in renal transplantation. Polymorphisms with functional significance in the promoter and coding regions of cytokine genes have been suggested as a possible factor for graft rejection. The aim of this study was to investigate the impact of cytokine gene polymorphism of pro and anti-inflammatory cytokines on development of CAN in a group of renal transplant patients and donors. Eight single nucleotide polymorphisms (SNPs) including TNFA (-308), TGFB1 (cdns10, 25), IL-10 (-1082, -819, -592), IL-6 (-174) and IFNG (+874) were analyzed in 56 patients with stable graft function (SGF), 10 with CAN and 28 kidney donors by PCR-SSP method. CAN was significantly associated with the recipient TGFB1 cod10 T/T and combination of cods10, 25 T/T G/G genotypes (high producer), (p<0.05). Influence of patient's TNFA genotype correlated with high level of gene expression on the development of CAN was further demonstrated when the patients were stratified according to the HLA mismatches (HLA-DRB MMs). Additionally donor TNFA-308 G/A (high) and IL-6-174 CC (low) genotypes were increased in cases with CAN. No statistically significant differences in distribution of IL-10, IL-6 and IFNG genotypes between recipients with SGF and CAN were found. In conclusion our data suggest that the high producer genotype of profibrogenetic TGF-beta1, pro-inflammatory TNF-alpha and genetically determined low production of immunoregulatory IL-6 cytokine might be risk factors for CAN development.
K ABSTRACT: The purpose of this study was to define the incidence, dynamics, and profiles of anti... more K ABSTRACT: The purpose of this study was to define the incidence, dynamics, and profiles of anti–human leukocyte antigen antibodies (HLA-Abs) produced after kidney transplantation and their impact on graft outcome. A total of 72 first cadaver donor kidney recipients were prospectively monitored for the development of HLA-Abs using beadbased flow-cytometry assays (One Lambda FlowPRA tests). Sixteen recipients (22.2%) developed HLA-Abs after transplantation (class I, n 7; class I II, n 6; class II, n 3), in most cases (81.25%) within the first 2 weeks posttransplantation. A strong association between alloantibody presence and delayed graft function (Chi-square 7.659, p 0.01), acute rejection (Chi-square 14.504, p 0.001), chronic rejection (Chi-square 12.84, p 0.001), and graft loss (Chi-square 20.283, p 0.001) was found. Patients with higher alloantibody titers experienced acute rejections and even early graft loss, compared with those r
The aerobiological study of the Ambrosia pollen in Sofia, Bulgaria began in 1991. The quantitativ... more The aerobiological study of the Ambrosia pollen in Sofia, Bulgaria began in 1991. The quantitative dynamics of Ambrosia pollen grains during the last seven years was determined by Chebishev's method of parabolic interpolation with orthogonal polynoms. Thesensitization to ragweed pollen was investigated in random groups of patients with clinical manifestation of hay fever.
Chronic allograft nephropathy (CAN) is one of the main causes of graft loss in renal transplantat... more Chronic allograft nephropathy (CAN) is one of the main causes of graft loss in renal transplantation. Polymorphisms with functional significance in the promoter and coding regions of cytokine genes have been suggested as a possible factor for graft rejection. The aim of this study was to investigate the impact of cytokine gene polymorphism of pro and anti-inflammatory cytokines on development of CAN in a group of renal transplant patients and donors. Eight single nucleotide polymorphisms (SNPs) including TNFA (-308), TGFB1 (cdns10, 25), IL-10 (-1082, -819, -592), IL-6 (-174) and IFNG (+874) were analyzed in 56 patients with stable graft function (SGF), 10 with CAN and 28 kidney donors by PCR-SSP method. CAN was significantly associated with the recipient TGFB1 cod10 T/T and combination of cods10, 25 T/T G/G genotypes (high producer), (p<0.05). Influence of patient's TNFA genotype correlated with high level of gene expression on the development of CAN was further demonstrated when the patients were stratified according to the HLA mismatches (HLA-DRB MMs). Additionally donor TNFA-308 G/A (high) and IL-6-174 CC (low) genotypes were increased in cases with CAN. No statistically significant differences in distribution of IL-10, IL-6 and IFNG genotypes between recipients with SGF and CAN were found. In conclusion our data suggest that the high producer genotype of profibrogenetic TGF-beta1, pro-inflammatory TNF-alpha and genetically determined low production of immunoregulatory IL-6 cytokine might be risk factors for CAN development.
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Papers by Daniela Baltadjieva