Papers by David Wood Cano
ABSTRACT La necesidad de interoperabilidad en los sistemas públicos de salud como soporte a la co... more ABSTRACT La necesidad de interoperabilidad en los sistemas públicos de salud como soporte a la continuidad asistencial está plenamente establecida desde hace ya algún tiempo y el uso de la normalización se ha situado como la principal estrategia para conseguirla. Este texto pretende ser un manual práctico para dotar a los profesionales de la salud tanto tecnológicos como sanitarios , pues ambos están involucrados en el proceso, de las herramientas necesarias para comprender y acometer la interoperabilidad, en el más amplio sentido del término, de sus organizaciones.
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ABSTRACT La necesidad de interoperabilidad en los sistemas públicos de salud como soporte a la co... more ABSTRACT La necesidad de interoperabilidad en los sistemas públicos de salud como soporte a la continuidad asistencial está plenamente establecida desde hace ya algún tiempo y el uso de la normalización se ha situado como la principal estrategia para conseguirla. Este texto pretende ser un manual práctico para dotar a los profesionales de la salud tanto tecnológicos como sanitarios , pues ambos están involucrados en el proceso, de las herramientas necesarias para comprender y acometer la interoperabilidad, en el más amplio sentido del término, de sus organizaciones.
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Molecular Microbiology, 2004
Bacterial pathogenesis relies on regulators that activate virulence genes. Some of them act, in a... more Bacterial pathogenesis relies on regulators that activate virulence genes. Some of them act, in addition, as repressors of specific genes. Intracellular-growth-attenuator-A (IgaA) is a Salmonella enterica membrane protein that prevents overactivation of the RcsC-YojN-RcsB regulatory system. This negative control is critical for growth because disruption of the igaA gene is only possible in rcsC, yojN or rcsB strains. In this work, we examined the contribution of this regulatory circuit to virulence. Viable igaA point mutant alleles were isolated and characterized. These alleles encode IgaA variants leading to different levels of activation of the RcsC-YojN-RcsB system. IgaA-mediated repression of the RcsB-YojN-RcsC system occurred at the post-translational level, as shown by chromosomal epitope tagging of the rcsC, yojN and rcsB genes. The activity of the RcsC-YojN-RcsB system, monitored with the product of a tagged gmd-3xFLAG gene (positively regulated by RcsC-YojN-RcsB), was totally abolished by wild-type bacteria in mouse target organs. Such tight repression occurred only in vivo and was mediated by IgaA. Shutdown of the RcsC-YojN-RcsB system is a requisite for Salmonella virulence since all igaA point mutant strains were highly attenuated. The degree of attenuation correlated to that of the activation status of RcsC-YojN-RcsB. In some cases, the attenuation recorded was unprecedented, with competitive index (CI) values as low as 10(-6). Strikingly, IgaA is a protein absolutely dispensable for virulence in mutant strains having a non-functional RcsC-YojN-RcsB system. To our knowledge, IgaA exemplifies the first protein that contributes to virulence by exclusively acting as a negative regulator upon host colonization.
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Journal of bacteriology, 1998
Histidine-constitutive (Hisc) strains of Salmonella typhimurium undergo cell division inhibition ... more Histidine-constitutive (Hisc) strains of Salmonella typhimurium undergo cell division inhibition in the presence of high concentrations of a metabolizable carbon source. Filaments formed by Hisc strains show constrictions and contain evenly spaced nucleoids, suggesting a defect in septum formation. Inhibitors of penicillin-binding protein 3 (PBP3) induce a filamentation pattern identical to that of Hisc strains. However, the Hisc septation defect is caused neither by reduced PBP3 synthesis nor by reduced PBP3 activity. Gross modifications of peptidoglycan composition are also ruled out. D-Cycloserine, an inhibitor of the soluble pathway producing peptidoglycan precursors, causes phenotypic suppression of filamentation, suggesting that the septation defect of Hisc strains may be caused by scarcity of PBP3 substrate.
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Frontiers in Oncology, 2014
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Endocrinología y Nutrición, 2014
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Development, 2013
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American Journal of Respiratory and Critical Care Medicine, 2005
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The astronomical, geographical and calendrical properties of one of the most famous Mayan pyramid... more The astronomical, geographical and calendrical properties of one of the most famous Mayan pyramid are introduced in the cultural Mesoamerican context.
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Publicaciones by David Wood Cano
ISSN: 0188-8218, May 2014
El Castillo constituye un magnífico ejemplo del ingenio y habilidad de los sacerdotes-astrónomos ... more El Castillo constituye un magnífico ejemplo del ingenio y habilidad de los sacerdotes-astrónomos mayas para conjuntar armónicamente sus conocimientos calendáricos y arquitectónicos con su propia cosmovisión. Se trata de un excepcional edificio que se manifiesta de una manera majestuosa como un monumento al tiempo, revelando un mensaje simbólico de la máxima sacralidad.
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Papers by David Wood Cano
Publicaciones by David Wood Cano