Cardiovascular diseases, obesity, diabetes, insulin resistance, cancer and metabolic syndrome, wh... more Cardiovascular diseases, obesity, diabetes, insulin resistance, cancer and metabolic syndrome, which are closely associated with high accumulation of triglycerides in the body tissues, remain serious causes of human mortality around the world. This is in part due to lack of rapid and sensitive methods to quantify the different fatty acids/triglycerides associated with these diseases. Current methods used by clinicians for serum analyses fail to report the quantitative values of the individual major polyunsaturated fatty acids (PUFAs) in molar concentrations but rather express them in percentages. Moreover, low-density lipoprotein-cholesterol (LDL-C) a major risk for cardiovascular diseases is estimated from Friedewald Formula (FF). This formula is known to inaccurately estimate the levels of LDL-C especially when serum triglyceride (TG) concentrations of greater than 4.52 mmol/L are obtained. Therefore, a method that can effectively quantify the serum PUFAs and cholesterol simultane...
The ratio of ω-6 to ω-3 fatty acids as a biomarker for many clinical conditions such as breast ca... more The ratio of ω-6 to ω-3 fatty acids as a biomarker for many clinical conditions such as breast cancer, coronary cancer, and cardiac arrest has become an issue of concern to many researchers (1–4). ω-3 fatty acids are essential for human development and also are most helpful for achieving and sustaining good health (5). An acceptable balance between the fractions is essential for development of human beings (6). Artemis and colleagues (1), for example, suggested that the most important aspect of polyunsaturated fatty acids (PUFAs) in the prevention of cancer is the PUFA ratio rather than the absolute amount of either. Many studies have found that the ratios in Western diets lie between 10 to 20 (1–4). Epidemiological and experimental research indicates that a ratio ranging from 1/1 to 2/1 has a protective effect against the development and growth of breast and colon cancers. Short-term biomarker studies in human beings suggest that ω-3 PUFA supplemented at an ω-6/ω-3 ratio of about 2...
Quantitative determinations of polyunsaturated fatty acids (PUFAs) in human serum have been a gre... more Quantitative determinations of polyunsaturated fatty acids (PUFAs) in human serum have been a great challenge. The quantification of individual omega 3 (ω-3) and omega 6 (ω-6) fractions has not been accomplished to date. Instruments used for the analyses of PUFAS include gas chromatography (GC), thin layer chromatography (TLC), and high-performance liquid chromatography (HPLC) that obtain the total amount of PUFAs instead of individual PUFAs or the ω-6/ω-3 ratios (1, 2). At the same time, the GC results are reported as relative percentages of the calculated total peak areas or peak heights that are prone to change with the number of the integrated peaks (3). This method of expressing data has been shown to be less accurate than absolute concentration data and of lesser meaning than ω-6/ω-3 ratios for clinical applications (4-7). On the other hand, typical hospital diagnoses report the saturated fatty acids and PUFA data as total triglycerides, ignoring the fact that triglycerides sh...
Cardiovascular diseases, obesity, diabetes, insulin resistance, cancer and metabolic syndrome, wh... more Cardiovascular diseases, obesity, diabetes, insulin resistance, cancer and metabolic syndrome, which are closely associated with high accumulation of triglycerides in the body tissues, remain serious causes of human mortality around the world. This is in part due to lack of rapid and sensitive methods to quantify the different fatty acids/triglycerides associated with these diseases. Current methods used by clinicians for serum analyses fail to report the quantitative values of the individual major polyunsaturated fatty acids (PUFAs) in molar concentrations but rather express them in percentages. Moreover, low-density lipoprotein-cholesterol (LDL-C) a major risk for cardiovascular diseases is estimated from Friedewald Formula (FF). This formula is known to inaccurately estimate the levels of LDL-C especially when serum triglyceride (TG) concentrations of greater than 4.52 mmol/L are obtained. Therefore, a method that can effectively quantify the serum PUFAs and cholesterol simultane...
The ratio of ω-6 to ω-3 fatty acids as a biomarker for many clinical conditions such as breast ca... more The ratio of ω-6 to ω-3 fatty acids as a biomarker for many clinical conditions such as breast cancer, coronary cancer, and cardiac arrest has become an issue of concern to many researchers (1–4). ω-3 fatty acids are essential for human development and also are most helpful for achieving and sustaining good health (5). An acceptable balance between the fractions is essential for development of human beings (6). Artemis and colleagues (1), for example, suggested that the most important aspect of polyunsaturated fatty acids (PUFAs) in the prevention of cancer is the PUFA ratio rather than the absolute amount of either. Many studies have found that the ratios in Western diets lie between 10 to 20 (1–4). Epidemiological and experimental research indicates that a ratio ranging from 1/1 to 2/1 has a protective effect against the development and growth of breast and colon cancers. Short-term biomarker studies in human beings suggest that ω-3 PUFA supplemented at an ω-6/ω-3 ratio of about 2...
Quantitative determinations of polyunsaturated fatty acids (PUFAs) in human serum have been a gre... more Quantitative determinations of polyunsaturated fatty acids (PUFAs) in human serum have been a great challenge. The quantification of individual omega 3 (ω-3) and omega 6 (ω-6) fractions has not been accomplished to date. Instruments used for the analyses of PUFAS include gas chromatography (GC), thin layer chromatography (TLC), and high-performance liquid chromatography (HPLC) that obtain the total amount of PUFAs instead of individual PUFAs or the ω-6/ω-3 ratios (1, 2). At the same time, the GC results are reported as relative percentages of the calculated total peak areas or peak heights that are prone to change with the number of the integrated peaks (3). This method of expressing data has been shown to be less accurate than absolute concentration data and of lesser meaning than ω-6/ω-3 ratios for clinical applications (4-7). On the other hand, typical hospital diagnoses report the saturated fatty acids and PUFA data as total triglycerides, ignoring the fact that triglycerides sh...
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