There is an increased and continuous need for developing new methods for the separation and quant... more There is an increased and continuous need for developing new methods for the separation and quantification of an increasing number of analytes in the environmental, pharmaceutical, pharmacological, and toxicological sciences. CEC is still withholding its popularity, representing a viable alternative to the more conventional techniques (HPLC, GC) due to the numerous advantages, such as, low sample/reagent volumes, high separation efficiencies, hybrid separation principle, etc. One particular promising direction in CEC is the use of molecularly imprinted polymers (MIPs) as stationary phases. They are usually immobilized in the capillary column as a continuous polymeric monolith or as a thin polymer coating attached to the capillary's inner wall. Another emerging trend is the use of MIPs in the form of nanoparticles as a pseudostationary phase. This review discusses the recent developments (2011-2013) in finding the optimal polymerization mixture and the suitable MIP format that should be employed in CEC separations. The most important applications of MIPs in CEC technique are also highlighted.
A simple, fast and accurate method of chiral recognition and quantification of propranolol enanti... more A simple, fast and accurate method of chiral recognition and quantification of propranolol enantiomers by surface enhanced Raman scattering (SERS) and multivariate regression analysis through supramolecular interaction with β-cyclodextrin is reported. Computational chemistry served as a tool of elucidating the underlying mechanism of molecular interactions responsible for chiral discrimination. The influence of several factors (nature and concentration of chiral auxiliary, selector-selectand ratio, pH, interaction time, etc.) over the obtained SERS spectra was assessed, followed by the construction of the chemometric model with the optimized operational conditions. The performance of the obtained semi-empirical model was established using a validation set of pure enantiomers and its intended use was demonstrated by the assessment of the enantiomeric excess of propranolol in pharmaceutical formulations (tablets) without the need of tedious and expensive chiral separation. The obtained results were also confirmed by chiral high-performance liquid chromatography.
There is an increased and continuous need for developing new methods for the separation and quant... more There is an increased and continuous need for developing new methods for the separation and quantification of an increasing number of analytes in the environmental, pharmaceutical, pharmacological, and toxicological sciences. CEC is still withholding its popularity, representing a viable alternative to the more conventional techniques (HPLC, GC) due to the numerous advantages, such as, low sample/reagent volumes, high separation efficiencies, hybrid separation principle, etc. One particular promising direction in CEC is the use of molecularly imprinted polymers (MIPs) as stationary phases. They are usually immobilized in the capillary column as a continuous polymeric monolith or as a thin polymer coating attached to the capillary's inner wall. Another emerging trend is the use of MIPs in the form of nanoparticles as a pseudostationary phase. This review discusses the recent developments (2011-2013) in finding the optimal polymerization mixture and the suitable MIP format that should be employed in CEC separations. The most important applications of MIPs in CEC technique are also highlighted.
A simple, fast and accurate method of chiral recognition and quantification of propranolol enanti... more A simple, fast and accurate method of chiral recognition and quantification of propranolol enantiomers by surface enhanced Raman scattering (SERS) and multivariate regression analysis through supramolecular interaction with β-cyclodextrin is reported. Computational chemistry served as a tool of elucidating the underlying mechanism of molecular interactions responsible for chiral discrimination. The influence of several factors (nature and concentration of chiral auxiliary, selector-selectand ratio, pH, interaction time, etc.) over the obtained SERS spectra was assessed, followed by the construction of the chemometric model with the optimized operational conditions. The performance of the obtained semi-empirical model was established using a validation set of pure enantiomers and its intended use was demonstrated by the assessment of the enantiomeric excess of propranolol in pharmaceutical formulations (tablets) without the need of tedious and expensive chiral separation. The obtained results were also confirmed by chiral high-performance liquid chromatography.
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Papers by Ede Bodoki