Polythenes are used in many spheres of human life such as packing of commodities and in construct... more Polythenes are used in many spheres of human life such as packing of commodities and in construction of green houses and ponds among other uses. As a result when not properly disposed, they contaminate our environment since they are not easily biodegraded. In this study, some polythene papers were buried around growing maize, in the forest and in waste damping site. At the same time, soil samples were separately collected from the three sites, packed in sterilised polythene bags and taken to the laboratory for isolation of actinomycetes. In the laboratory, the soils were dried on the bench for one week. Following this, actinomycetes were isolated using standard methods using starch casein agar. Characterization of the isolates was carried out using cultural, physiological and biochemical means. The polythene were subjected to degradation by the actinomycetes by placing them in conical flasks having starch casein broth and incubating them in shaking conditions at 30 o C for one week....
2021 ASABE Annual International Virtual Meeting, July 12-16, 2021, 2021
Abstract. Curcumin has diverse pharmacological applications as anti-inflammatory, anti-cancer, an... more Abstract. Curcumin has diverse pharmacological applications as anti-inflammatory, anti-cancer, anti-fungal, and antibacterial. However, high hydrophobicity, rapid metabolism, and low bioavailability limit its application. Encapsulation improves its application by enhancing the bioavailability, stability, and solubility of this active compound. The study's objective was to investigate the ultrasonication effect and the compositional parameters on the physical attributes and encapsulation efficiency (EE) of the curcumin nanoparticles (NP). Through the emulsion evaporation method, (CN) were loaded into (poly-lactic acid -PLA). The selected parameters: curcumin concentration, surfactant concentration, sonication amplitude, and time were performed to achieve the highest nanoparticle attributes. Curcumin concentration of 0.2, 0.4, and 0.6 mg/mL at sonication amplitude of 80 and 100 µm with varying sonication time was used to prepare CN. The CN encapsulated in PLA (CNPLA) was characterized by a differential scanning calorimeter. A dynamic light scattering instrument was used to determine the Poly-dispersity Index (PDI), average hydrodynamic diameter (HD), and the zeta potential (ZP) and EE determined by UV vis-spectrophotometer. The results show that an increase in amplitude and 5 minutes sonication time resulted in significant (p
Polythenes are used in many spheres of human life such as packing of commodities and in construct... more Polythenes are used in many spheres of human life such as packing of commodities and in construction of green houses and ponds among other uses. As a result when not properly disposed, they contaminate our environment since they are not easily biodegraded. In this study, some polythene papers were buried around growing maize, in the forest and in waste damping site. At the same time, soil samples were separately collected from the three sites, packed in sterilised polythene bags and taken to the laboratory for isolation of actinomycetes. In the laboratory, the soils were dried on the bench for one week. Following this, actinomycetes were isolated using standard methods using starch casein agar. Characterization of the isolates was carried out using cultural, physiological and biochemical means. The polythene were subjected to degradation by the actinomycetes by placing them in conical flasks having starch casein broth and incubating them in shaking conditions at 30 o C for one week....
2021 ASABE Annual International Virtual Meeting, July 12-16, 2021, 2021
Abstract. Curcumin has diverse pharmacological applications as anti-inflammatory, anti-cancer, an... more Abstract. Curcumin has diverse pharmacological applications as anti-inflammatory, anti-cancer, anti-fungal, and antibacterial. However, high hydrophobicity, rapid metabolism, and low bioavailability limit its application. Encapsulation improves its application by enhancing the bioavailability, stability, and solubility of this active compound. The study's objective was to investigate the ultrasonication effect and the compositional parameters on the physical attributes and encapsulation efficiency (EE) of the curcumin nanoparticles (NP). Through the emulsion evaporation method, (CN) were loaded into (poly-lactic acid -PLA). The selected parameters: curcumin concentration, surfactant concentration, sonication amplitude, and time were performed to achieve the highest nanoparticle attributes. Curcumin concentration of 0.2, 0.4, and 0.6 mg/mL at sonication amplitude of 80 and 100 µm with varying sonication time was used to prepare CN. The CN encapsulated in PLA (CNPLA) was characterized by a differential scanning calorimeter. A dynamic light scattering instrument was used to determine the Poly-dispersity Index (PDI), average hydrodynamic diameter (HD), and the zeta potential (ZP) and EE determined by UV vis-spectrophotometer. The results show that an increase in amplitude and 5 minutes sonication time resulted in significant (p
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Papers by Edwin Ochieng