The aim of this study was to assess the hygiene of pork, beef, and poultry carcasses and to deter... more The aim of this study was to assess the hygiene of pork, beef, and poultry carcasses and to determine the phenotypic antibiotic susceptibility of the bacteria embedded in the biofilm formed on the carcasses kept in cooling chambers for at least three days. The level of hygiene was assessed by determining the total aerobic colony count (TACC) and the Enterobacteriaceae level in different sampling points of the carcasses, along with the detection of E. coli and Pseudomonas spp. embedded in the biofilm. Furthermore, the E. coli and Pseudomonas spp. isolates were tested for antimicrobial resistance profiles. A total of 130 samples collected from pork, beef, and poultry from processing units were analyzed to determine the total aerobic colony count as well as to measure the level of Enterobacteriaceae found on the carcasses. The antimicrobial susceptibility of 44 Escherichia coli and eight Pseudomonas spp. strains isolated from the carcasses were assessed using the Vitek 2 system using t...
Anise (Pimpinella anisum L.) essential oils are intensely investigated worldwide for the benefici... more Anise (Pimpinella anisum L.) essential oils are intensely investigated worldwide for the beneficial properties, due to the specific bioactive compound’s structure. (1) Background: This study characterized the structure of the Pimpinella anisum essential oil and evaluated its antimicrobial properties. (2) Methods: An evaluation of the antibacterial and antifungal activity targeted strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19615), and levure Candida albicans (ATCC 10231). Gas chromatography coupled with mass spectrometry (GC/MS) was used for structure identification, and the optical density mass loss was applied for the analysis of different dilutions of aniseed essential oils antimicrobial activity. (3) Results: A total of 13 compounds were identified, of which trans-anethole was in the highest proportion (72.49%), followed by limonene (10.01%), anisole (5%), and α-pinene (3.26%). Th...
This study aimed to investigate the antioxidant profile and the antimicrobial activity of four di... more This study aimed to investigate the antioxidant profile and the antimicrobial activity of four different types of monofloral honey (manuka (MH), brassica rapeseed (BH), acacia (AH), and linden honey (LH)) against some bacterial/fungal ATCC strains and some multidrug-resistant strains isolated from chronic otitis in dogs. For the characterisation of the antioxidant profile of each honey, we extracted the honey samples by hydroalcoholic extraction and analysed them in terms of total polyphenols (TPC), total flavonoids (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) using the spectrophotometric method. The antimicrobial activity was determined using the microdilution method at concentrations of 10%, 15%, and 20%, with the results expressed in OD (optical density) calculated as BIR% (bacterial inhibition rate)/MIR% (mycelial inhibition rate). The antioxidant characterisation of the analysed honey samples showed the highest antioxidant activity and concentrations of TPC and TFC in MH, fo...
Bird flu or avian influenza continues to cause problems at European level, with new outbreaks of ... more Bird flu or avian influenza continues to cause problems at European level, with new outbreaks of disease being reported, including those identified in January 2020 in Romania. It was first identified in the 1900s in Italy, but was later found worldwide. An avian influenza virus, H5N1, was identified in 1997 in Asia and created worldwide panic, talking about the imminence of a major epidemic. In October 2005, the H5N1 virus also arrived in Romania, being identified in birds from Ceamurlia de Jos, Tulcea County. Romania is the first European country where the presence of this virus has been officially confirmed. Avian influenza has attracted the attention of the international community over the years, with outbreaks in the poultry industry or livelihoods having serious consequences on both livelihoods and international trade in many countries. Apart from Romania, avian influenza is still evolving in Poland, Slovakia, Hungary, Germany, Ukraine and Northern Ireland. Preventing the occur...
In this study we determined the immunostimulant effect of an alcoholic total extract from Pleurot... more In this study we determined the immunostimulant effect of an alcoholic total extract from Pleurotus ostreatus administered in 5 and 15% concentration to broiler chickens vaccinated against infectious bursal disease. The immunogen was represented by two vaccines (Biavac and Biaromvac) used in infectious bursal disease prophylaxis. Vaccination was made at the age of seven days in four groups of broilers. Laboratory tests results showed that fungal extract have a stimulant action upon average daily gain, the most conclusive results being registered in group treated with 15% Pleurotus extract.
BACKGROUND: Infection with hepatitis-B virus has been a significant cause of morbidity claiming m... more BACKGROUND: Infection with hepatitis-B virus has been a significant cause of morbidity claiming more than a million lives every year. Epidemiological data reveals that there are 360 million carriers of hepatitis-B virus throughout the globe and 78% of the world populations’ hail from Asia. Though several studies from Indian sub-continent have provided an estimate of the prevalence of this viral infection, there exist only few studies, which reflect the status in the general population. AIM: The present study was designed to investigate the prevalence of hepatitis-B infection in North Indian general population. METHODS: The study population comprised of 20,000 healthy blood donors who were screened for hepatitis-B surface antigen (HBsAg) status using third generation ELISA kit. Seroprevalence rate of seropositive donors was calculated and stratified by age, sex and blood groups. Statistical analysis was performed using tests of proportions, chi-square and confidence interval. RESULTS...
The study was undertaken to investigate the main causes of carcass and organ condemnations, as we... more The study was undertaken to investigate the main causes of carcass and organ condemnations, as well as to estimate the financial losses suffered by a cattle slaughterhouse. In this regard, an active abattoir survey, based on standard post-mortem inspection procedures for meat, was conducted on 151,741 cattle, from January 2021 to December 2022. Overall, 13.27% (n = 20,125) of the carcasses expressed lesions or pathological conditions and, out of them, 1.15% (n = 1738) were totally confiscated, while another 12.12% (n = 18,387) were partially admitted for human consumption. In the case of organs, the general inspection data reveal that 12.28% (n = 18,630), 7.56% (n = 11,477), 1.89% (n = 2862), and 0.27% (n = 412) of the examined liver, lung, heart, and kidney specimens presented one or more types of abnormalities. In addition, regarding the types of specific pathological findings, dystrophies/anomalies (69.8%), circulatory disorders (40.6%), fecal contamination (60.9%), and suspected...
This study aimed to investigate the chemical composition and the activity against Staphylococcus ... more This study aimed to investigate the chemical composition and the activity against Staphylococcus aureus (S. aureus) (ATCC 25923), Streptococcus pyogenes (S. pyogenes) (ATCC 19615), Escherichia coli (E. coli) (ATCC 25922), Pseudomonas aeruginosa (P. aeruginosa) (ATCC 27853), Shigella flexneri (S. flexneri) (ATCC 12022), Salmonella typhimurium (S. typhimurium) (ATCC 14028), Haemophillus influenzae (H. influenza) type B (ATCC 10211) and two fungal strains: Candida albicans (C. albicans) (ATCC 10231) and Candida parapsilopsis (C. parapsilopsis) (ATCC 22019) of the extracts obtained from Melilotus officinalis (MO), Coronilla varia (CV); Ononis spinosa (OS) and Robinia pseudoacacia (RP) (Fabaceae), and to identify the chemical compounds responsible for the antimicrobial effect against the tested strains. The extracts were obtained by conventional hydroalcoholic extraction and analyzed in terms of total polyphenols using the spectrophotometric method and by liquid chromatography (LC). The ...
The study was been performed to emphasizing the dynamics of immunological parameters in healthy a... more The study was been performed to emphasizing the dynamics of immunological parameters in healthy and parasitically infested youth sheep from different private herds from Timis district. The researches were been made on20 youth sheep 10-11 months aged from Ţurcana breed divided in two groups: first group (M) comprised 10 healthy sheep periodic treated with antiparasitic drugs and the second group (E) contained 10 sheep who never received any antiparasitic drugs. All animals are clinical healthy, but those of E group are more skinny and have long and bristled fleece. From both groups were been taken blood samples in the view of evaluation the dynamics of unspecific immune response, represented by some parameters, like seric properdine, seric lysozime, phagocytic index and leucogramme. The obtained results confirm that immune system in infected animals always tried to counteract the noxious action of parasitic agents through increased values of studied parameters. A coproscopic examinat...
Reptiles are potential reservoirs of bacteria that could be transmitted, thus becoming a zoonotic... more Reptiles are potential reservoirs of bacteria that could be transmitted, thus becoming a zoonotic hazard. (1) Background: This three-year investigation surveyed the pathological status of 398 pet reptiles: chelonians, snakes (venomous/non-venomous), and lizards. The main pathological entities found were related to the skin, the sensory organs, the digestive system, the respiratory system, the cardiovascular system, the urinary system, the genitalia, the osteo–muscular tract, surgical issues, tumors, and intoxications. (2) Methods: In 25 individuals treated with antibiotics, no clinical healing was recorded, for this reason, an antimicrobial resistance profile analysis of the 43 samples gathered was processed. An antibiogram was performed using the VITEK®2 ID-GP (bio-Mérieux, Marcy l’Etoile, France) automated platform, with 22 bacterial strains being isolated. (3) Results: The statistics (ANOVA) revealed that the most common disease category was diseases of the digestive system, foll...
IOP Conference Series: Earth and Environmental Science, 2021
Wheat (Triticum aestivum L.) is the basic cereal in human and animal nutrition. Every month, whea... more Wheat (Triticum aestivum L.) is the basic cereal in human and animal nutrition. Every month, wheat is harvested somewhere in the world. In Romania, a country with a temperate-continental climate, the wheat is harvested between June and July, while the sowing is carried out between September and October. Climatic and meteorological factors during these periods can influence the nutritional quality of wheat. The aim of this study was to analyse the influence of annual average temperature and the amount of precipitate on the chemical composition and on the value of metabolizable energy of the wheat, respectively. The climatic and meteorological data used in this study come from NMA database. Were analysed the periods September 2017 - July 2018 (period 1, noted with 2018 - the year of harvesting) and September 2018 - July 2019 (period 2, noted with 2019 - the year of harvesting), respectively. For the chemical analysis, the NIR (Near InfraRed spectroscopy) method was used. The calculati...
Biofilm forming bacteria in oral cavity of dogs have an important impact on human health because ... more Biofilm forming bacteria in oral cavity of dogs have an important impact on human health because they can transmit zoonotic infection after dog bites. Knowing the microbial composition of dental plaque biofilm is crucial for understanding oral and systemic diseases in dogs but also those transmitted to humans. Recently it was found that biofilm is a key factor in delaying wound healing process of the skin. The skin is an essential component of nonspecific immune system, protecting the host of potential pathogens from the environment. Thus, breaches that appear in this protective barrier are a form of compromised immunity, which predisposes the patient to infection. Bites and scratches caused by animals and humans can inoculate microorganisms over the protective barrier of the skin. The objective of this study is to reveal that microbiology of infected wounds produced by dog bites is very similar to that of oral cavity in dogs. Delaying wound healing process is achieved by the fact t...
Germs from Yersinia genus have a vast ecologic niche, being met at different domestic and wild an... more Germs from Yersinia genus have a vast ecologic niche, being met at different domestic and wild animal species, but also in food, water and soil. The majority of yersinis live in the digestive tract of human and numerous animal species, especially rodents, but also in soil, plant debris, waters etc. Numerous species of Yersinia genus could produce characteristic infections in human, the main source of infections is represented by rodents and hematophagous insects or, more frequently, by water or contaminated food. In a 1999 study, Mead and coauthors established that the Yersinia enterocolitica prevalence in food, in USA, is around 90%. Foods of animal origin more frequently contaminated with Yersinia enterocolitica are: pork, poultry, beef and lamb meat, milk, ice-cream, sea fruits etc., among them pork meat and milk represents the sources of the most numerous toxi-infection outbreaks in human, in different world regions. Bacteria determine infections which interest the digestive tra...
The aim of this study was to assess the hygiene of pork, beef, and poultry carcasses and to deter... more The aim of this study was to assess the hygiene of pork, beef, and poultry carcasses and to determine the phenotypic antibiotic susceptibility of the bacteria embedded in the biofilm formed on the carcasses kept in cooling chambers for at least three days. The level of hygiene was assessed by determining the total aerobic colony count (TACC) and the Enterobacteriaceae level in different sampling points of the carcasses, along with the detection of E. coli and Pseudomonas spp. embedded in the biofilm. Furthermore, the E. coli and Pseudomonas spp. isolates were tested for antimicrobial resistance profiles. A total of 130 samples collected from pork, beef, and poultry from processing units were analyzed to determine the total aerobic colony count as well as to measure the level of Enterobacteriaceae found on the carcasses. The antimicrobial susceptibility of 44 Escherichia coli and eight Pseudomonas spp. strains isolated from the carcasses were assessed using the Vitek 2 system using t...
Anise (Pimpinella anisum L.) essential oils are intensely investigated worldwide for the benefici... more Anise (Pimpinella anisum L.) essential oils are intensely investigated worldwide for the beneficial properties, due to the specific bioactive compound’s structure. (1) Background: This study characterized the structure of the Pimpinella anisum essential oil and evaluated its antimicrobial properties. (2) Methods: An evaluation of the antibacterial and antifungal activity targeted strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19615), and levure Candida albicans (ATCC 10231). Gas chromatography coupled with mass spectrometry (GC/MS) was used for structure identification, and the optical density mass loss was applied for the analysis of different dilutions of aniseed essential oils antimicrobial activity. (3) Results: A total of 13 compounds were identified, of which trans-anethole was in the highest proportion (72.49%), followed by limonene (10.01%), anisole (5%), and α-pinene (3.26%). Th...
This study aimed to investigate the antioxidant profile and the antimicrobial activity of four di... more This study aimed to investigate the antioxidant profile and the antimicrobial activity of four different types of monofloral honey (manuka (MH), brassica rapeseed (BH), acacia (AH), and linden honey (LH)) against some bacterial/fungal ATCC strains and some multidrug-resistant strains isolated from chronic otitis in dogs. For the characterisation of the antioxidant profile of each honey, we extracted the honey samples by hydroalcoholic extraction and analysed them in terms of total polyphenols (TPC), total flavonoids (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) using the spectrophotometric method. The antimicrobial activity was determined using the microdilution method at concentrations of 10%, 15%, and 20%, with the results expressed in OD (optical density) calculated as BIR% (bacterial inhibition rate)/MIR% (mycelial inhibition rate). The antioxidant characterisation of the analysed honey samples showed the highest antioxidant activity and concentrations of TPC and TFC in MH, fo...
Bird flu or avian influenza continues to cause problems at European level, with new outbreaks of ... more Bird flu or avian influenza continues to cause problems at European level, with new outbreaks of disease being reported, including those identified in January 2020 in Romania. It was first identified in the 1900s in Italy, but was later found worldwide. An avian influenza virus, H5N1, was identified in 1997 in Asia and created worldwide panic, talking about the imminence of a major epidemic. In October 2005, the H5N1 virus also arrived in Romania, being identified in birds from Ceamurlia de Jos, Tulcea County. Romania is the first European country where the presence of this virus has been officially confirmed. Avian influenza has attracted the attention of the international community over the years, with outbreaks in the poultry industry or livelihoods having serious consequences on both livelihoods and international trade in many countries. Apart from Romania, avian influenza is still evolving in Poland, Slovakia, Hungary, Germany, Ukraine and Northern Ireland. Preventing the occur...
In this study we determined the immunostimulant effect of an alcoholic total extract from Pleurot... more In this study we determined the immunostimulant effect of an alcoholic total extract from Pleurotus ostreatus administered in 5 and 15% concentration to broiler chickens vaccinated against infectious bursal disease. The immunogen was represented by two vaccines (Biavac and Biaromvac) used in infectious bursal disease prophylaxis. Vaccination was made at the age of seven days in four groups of broilers. Laboratory tests results showed that fungal extract have a stimulant action upon average daily gain, the most conclusive results being registered in group treated with 15% Pleurotus extract.
BACKGROUND: Infection with hepatitis-B virus has been a significant cause of morbidity claiming m... more BACKGROUND: Infection with hepatitis-B virus has been a significant cause of morbidity claiming more than a million lives every year. Epidemiological data reveals that there are 360 million carriers of hepatitis-B virus throughout the globe and 78% of the world populations’ hail from Asia. Though several studies from Indian sub-continent have provided an estimate of the prevalence of this viral infection, there exist only few studies, which reflect the status in the general population. AIM: The present study was designed to investigate the prevalence of hepatitis-B infection in North Indian general population. METHODS: The study population comprised of 20,000 healthy blood donors who were screened for hepatitis-B surface antigen (HBsAg) status using third generation ELISA kit. Seroprevalence rate of seropositive donors was calculated and stratified by age, sex and blood groups. Statistical analysis was performed using tests of proportions, chi-square and confidence interval. RESULTS...
The study was undertaken to investigate the main causes of carcass and organ condemnations, as we... more The study was undertaken to investigate the main causes of carcass and organ condemnations, as well as to estimate the financial losses suffered by a cattle slaughterhouse. In this regard, an active abattoir survey, based on standard post-mortem inspection procedures for meat, was conducted on 151,741 cattle, from January 2021 to December 2022. Overall, 13.27% (n = 20,125) of the carcasses expressed lesions or pathological conditions and, out of them, 1.15% (n = 1738) were totally confiscated, while another 12.12% (n = 18,387) were partially admitted for human consumption. In the case of organs, the general inspection data reveal that 12.28% (n = 18,630), 7.56% (n = 11,477), 1.89% (n = 2862), and 0.27% (n = 412) of the examined liver, lung, heart, and kidney specimens presented one or more types of abnormalities. In addition, regarding the types of specific pathological findings, dystrophies/anomalies (69.8%), circulatory disorders (40.6%), fecal contamination (60.9%), and suspected...
This study aimed to investigate the chemical composition and the activity against Staphylococcus ... more This study aimed to investigate the chemical composition and the activity against Staphylococcus aureus (S. aureus) (ATCC 25923), Streptococcus pyogenes (S. pyogenes) (ATCC 19615), Escherichia coli (E. coli) (ATCC 25922), Pseudomonas aeruginosa (P. aeruginosa) (ATCC 27853), Shigella flexneri (S. flexneri) (ATCC 12022), Salmonella typhimurium (S. typhimurium) (ATCC 14028), Haemophillus influenzae (H. influenza) type B (ATCC 10211) and two fungal strains: Candida albicans (C. albicans) (ATCC 10231) and Candida parapsilopsis (C. parapsilopsis) (ATCC 22019) of the extracts obtained from Melilotus officinalis (MO), Coronilla varia (CV); Ononis spinosa (OS) and Robinia pseudoacacia (RP) (Fabaceae), and to identify the chemical compounds responsible for the antimicrobial effect against the tested strains. The extracts were obtained by conventional hydroalcoholic extraction and analyzed in terms of total polyphenols using the spectrophotometric method and by liquid chromatography (LC). The ...
The study was been performed to emphasizing the dynamics of immunological parameters in healthy a... more The study was been performed to emphasizing the dynamics of immunological parameters in healthy and parasitically infested youth sheep from different private herds from Timis district. The researches were been made on20 youth sheep 10-11 months aged from Ţurcana breed divided in two groups: first group (M) comprised 10 healthy sheep periodic treated with antiparasitic drugs and the second group (E) contained 10 sheep who never received any antiparasitic drugs. All animals are clinical healthy, but those of E group are more skinny and have long and bristled fleece. From both groups were been taken blood samples in the view of evaluation the dynamics of unspecific immune response, represented by some parameters, like seric properdine, seric lysozime, phagocytic index and leucogramme. The obtained results confirm that immune system in infected animals always tried to counteract the noxious action of parasitic agents through increased values of studied parameters. A coproscopic examinat...
Reptiles are potential reservoirs of bacteria that could be transmitted, thus becoming a zoonotic... more Reptiles are potential reservoirs of bacteria that could be transmitted, thus becoming a zoonotic hazard. (1) Background: This three-year investigation surveyed the pathological status of 398 pet reptiles: chelonians, snakes (venomous/non-venomous), and lizards. The main pathological entities found were related to the skin, the sensory organs, the digestive system, the respiratory system, the cardiovascular system, the urinary system, the genitalia, the osteo–muscular tract, surgical issues, tumors, and intoxications. (2) Methods: In 25 individuals treated with antibiotics, no clinical healing was recorded, for this reason, an antimicrobial resistance profile analysis of the 43 samples gathered was processed. An antibiogram was performed using the VITEK®2 ID-GP (bio-Mérieux, Marcy l’Etoile, France) automated platform, with 22 bacterial strains being isolated. (3) Results: The statistics (ANOVA) revealed that the most common disease category was diseases of the digestive system, foll...
IOP Conference Series: Earth and Environmental Science, 2021
Wheat (Triticum aestivum L.) is the basic cereal in human and animal nutrition. Every month, whea... more Wheat (Triticum aestivum L.) is the basic cereal in human and animal nutrition. Every month, wheat is harvested somewhere in the world. In Romania, a country with a temperate-continental climate, the wheat is harvested between June and July, while the sowing is carried out between September and October. Climatic and meteorological factors during these periods can influence the nutritional quality of wheat. The aim of this study was to analyse the influence of annual average temperature and the amount of precipitate on the chemical composition and on the value of metabolizable energy of the wheat, respectively. The climatic and meteorological data used in this study come from NMA database. Were analysed the periods September 2017 - July 2018 (period 1, noted with 2018 - the year of harvesting) and September 2018 - July 2019 (period 2, noted with 2019 - the year of harvesting), respectively. For the chemical analysis, the NIR (Near InfraRed spectroscopy) method was used. The calculati...
Biofilm forming bacteria in oral cavity of dogs have an important impact on human health because ... more Biofilm forming bacteria in oral cavity of dogs have an important impact on human health because they can transmit zoonotic infection after dog bites. Knowing the microbial composition of dental plaque biofilm is crucial for understanding oral and systemic diseases in dogs but also those transmitted to humans. Recently it was found that biofilm is a key factor in delaying wound healing process of the skin. The skin is an essential component of nonspecific immune system, protecting the host of potential pathogens from the environment. Thus, breaches that appear in this protective barrier are a form of compromised immunity, which predisposes the patient to infection. Bites and scratches caused by animals and humans can inoculate microorganisms over the protective barrier of the skin. The objective of this study is to reveal that microbiology of infected wounds produced by dog bites is very similar to that of oral cavity in dogs. Delaying wound healing process is achieved by the fact t...
Germs from Yersinia genus have a vast ecologic niche, being met at different domestic and wild an... more Germs from Yersinia genus have a vast ecologic niche, being met at different domestic and wild animal species, but also in food, water and soil. The majority of yersinis live in the digestive tract of human and numerous animal species, especially rodents, but also in soil, plant debris, waters etc. Numerous species of Yersinia genus could produce characteristic infections in human, the main source of infections is represented by rodents and hematophagous insects or, more frequently, by water or contaminated food. In a 1999 study, Mead and coauthors established that the Yersinia enterocolitica prevalence in food, in USA, is around 90%. Foods of animal origin more frequently contaminated with Yersinia enterocolitica are: pork, poultry, beef and lamb meat, milk, ice-cream, sea fruits etc., among them pork meat and milk represents the sources of the most numerous toxi-infection outbreaks in human, in different world regions. Bacteria determine infections which interest the digestive tra...
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