La presente invention concerne un procede et un kit permettant de determiner les ruptures a doubles brins dans d'ADN. Ce procede consiste a mettre en contact un echantillon comprenant des proteines d'histone H2A avec... more
La presente invention concerne un procede et un kit permettant de determiner les ruptures a doubles brins dans d'ADN. Ce procede consiste a mettre en contact un echantillon comprenant des proteines d'histone H2A avec l'anticorps purifie ou isole ou un fragment de ce dernier presentant une reaction antigenique qui se lie, de maniere specifique, a la serine phosphorylee a terminaison C dans une proteine d'histone K2A. Ce procede consiste ensuite a detecter la liaison de l'anticorps ou du fragment de ce dernier a une proteine d'histone H2A dans l'echantillon. La detection de la liaison de l'anticorps ou du fragment de ce dernier a la proteine d'histone H2A indique la presence d'une rupture a double brins d'ADN dans l'ADN.
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Research Interests: Biology, Enzyme Inhibitors, Gamma Rays, DNA damage, DNA repair, and 15 moreMedicine, Biological Sciences, Humans, Lasers, Phosphorylation, Chromatin structure, Histones, Cell nucleus, Genomic DNA, Ionizing Radiation, Histone, DNA binding proteins, DNA double strand breaks, Double Strand Break Repair, and Medical and Health Sciences
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Cellular effects of ionizing radiation (IR) are of great variety and level, but they are mainly damaging since radiation can perturb all important components of the cell, from the membrane to the nucleus, due to alteration of different... more
Cellular effects of ionizing radiation (IR) are of great variety and level, but they are mainly damaging since radiation can perturb all important components of the cell, from the membrane to the nucleus, due to alteration of different biological molecules ranging from lipids to proteins or DNA. Regarding DNA damage, which is the main focus of this review, as well as its repair, all current knowledge indicates that IR-induced DNA damage is always more complex than the corresponding endogenous damage resulting from endogenous oxidative stress. Specifically, it is expected that IR will create clusters of damage comprised of a diversity of DNA lesions like double strand breaks (DSBs), single strand breaks (SSBs) and base lesions within a short DNA region of up to 15-20 bp. Recent data from our groups and others support two main notions, that these damaged clusters are: (1) repair resistant, increasing genomic instability (GI) and malignant transformation and (2) can be considered as pe...
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One of the most fascinating themes in the biology of double-strand breaks (DSBs) is that chromatin is emerging as a multifunctional player in the DSB damage response. The phosphorylation of H2AX on Ser 139, named γH2AX, is an early... more
One of the most fascinating themes in the biology of double-strand breaks (DSBs) is that chromatin is emerging as a multifunctional player in the DSB damage response. The phosphorylation of H2AX on Ser 139, named γH2AX, is an early response to the generation of DNA DSBs and extends along megabase-long domains, both sites of the lesion, supporting amplification of signal transduction pathways. In parallel, 53BP1 accumulates on damaged chromatin to interface between methylated histone residues and proteins that belong to the signal-transduction pathways, mediating cell-cycle arrest or apoptosis. Interestingly, the two pathways crosstalk at the chromatin level.
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In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (gamma-H2AX, which... more
In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (gamma-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'.
Research Interests: Fluorescence Microscopy, Biological Sciences, DNA, Saccharomyces cerevisiae, Antibodies, and 15 moreMeiosis, Mice, Female, Animals, Male, Proteins, Histones, Genetic Recombination, Spatial Correlation, Esterases, Cell Cycle Proteins, DNA binding proteins, Somatic Cells, DNA double strand breaks, and Medical and Health Sciences
In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (-H2AX, which marks... more
In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated
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Research Interests: Science, Confocal Microscopy, DNA damage, Multidisciplinary, T cell receptor, and 12 moreMice, Animals, Phosphorylation, Translocation, T lymphocytes, Histones, Cell nucleus, Fluorescent Antibody Technique, Genetic Recombination, Amino Acid Sequence, DNA binding proteins, and Molecular Sequence Data
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Research Interests: Chemistry, Biological Chemistry, Gamma Rays, Biological Sciences, DNA, and 13 moreProtein Kinases, Mice, Animals, Biological, Phosphorylation, Chromatin, CHEMICAL SCIENCES, Histones, Amino Acid Sequence, Recombinant Proteins, DNA double strand breaks, Molecular Sequence Data, and Medical and Health Sciences
Research Interests: DNA replication, Enzyme Inhibitors, Biological Chemistry, Confocal Microscopy, DNA damage, and 15 moreWestern blotting, Biological Sciences, Humans, Mice, Animals, Biological, Phosphorylation, CHEMICAL SCIENCES, Histones, Camptothecin, Cell Cycle Proteins, Hydrolysis, DNA binding proteins, DNA double strand breaks, and Medical and Health Sciences
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The loss of chromosomal integrity from DNA double-strand breaks introduced into mamma- lian cells by ionizing radiation results in the specific phosphorylation of histone H2AX on serine residue 139, yielding a specific modified form named... more
The loss of chromosomal integrity from DNA double-strand breaks introduced into mamma- lian cells by ionizing radiation results in the specific phosphorylation of histone H2AX on serine residue 139, yielding a specific modified form named g -H2AX. An antibody prepared to the unique region of human g -H2AX shows that H2AX homologues are phos- phorylated not only in irradiated mammalian