We have constructed a cDNA library from the highly hydroxyurea-resistant hamster cell line 600H i... more We have constructed a cDNA library from the highly hydroxyurea-resistant hamster cell line 600H in which the activity of ribonucleotide reductase is elevated more than 80-fold. Using the technique of differential hybridization, we have isolated a number of cDNA clones from this library which are homologous to genomic DNA sequences amplified in the 600H cell line compared to the V79 parental line. One of these cDNA clones by sequence analysis was found to code for ornithine decarboxylase. This was confirmed by in vitro translation of poly(A+) RNA isolated by hybridization-selection followed by immunoprecipitation with antiserum specific for mouse ornithine decarboxylase. Genomic sequences homologous to the cDNA clone were shown to be sequentially amplified 6-20-fold in hamster cell lines selected stepwise for resistance to increasing concentrations of hydroxyurea. Genomic sequences homologous to a cDNA for the M2 subunit of ribonucleotide reductase were also amplified in these cell lines, and the degree of M2 sequence amplification corresponded to the degree of amplification of ornithine decarboxylase sequences, suggesting that the two genes had been co-amplified during the selection of the hydroxyurea-resistant phenotype.
Journal of Agricultural and Environmental Ethics, 2021
This essay proposes African-based ethical solutions to profound human problems and a working Afri... more This essay proposes African-based ethical solutions to profound human problems and a working African model to address those problems. The model promotes sustainability through advanced agroecological and information communication technologies. The essay’s first section reviews the ethical ground of that model in the work of the Senegalese scholar, Cheikh Anta Diop. The essay’s second section examines an applied African model for translating African ethical speculation into practice. Deeply immersed in European and African ethics, Godfrey Nzamujo developed the Songhaï Centers to solve the problem of rural poverty in seventeen African countries. Harnessing advanced technologies within a holistic agroecological ecosystem, Nzamujo’s villages furnish education spanning the fields of ethics, information communication technology, microbiology, international development, and mechanical, electrical, civil and biological engineering in a community-based and centered development enterprise. Th...
But marketing is here to stay. So how can it be transformed to be an agent of sustainability? As ... more But marketing is here to stay. So how can it be transformed to be an agent of sustainability? As part of Friends of the Earth’s Big Ideas Project we have been looking at this question. And we have concluded that marketing has to evolve from a paradigm of ‘sense and provide’ to a new paradigm, which we call ‘guide and co-create (for sustainability)’. Central to achieving this evolution is, we suggest, a ‘framework for evolved marketing’.
Abstract. Futures Consciousness refers to the capacity that a person has for understanding, antic... more Abstract. Futures Consciousness refers to the capacity that a person has for understanding, anticipating, and preparing for the future. Although the concept is widely used in the field of futures r...
The hydroxyurea-resistant Chinese hamster cell line 600H has been shown to have greatly elevated ... more The hydroxyurea-resistant Chinese hamster cell line 600H has been shown to have greatly elevated quantities of ribonucleotide reductase. This increase in enzyme activity is due to an increased level of both the M1 and M2 subunit activities. The M1 subunit has been purified from the 600H cell line and shown to consist of a series of six protein spots with apparent molecular weights of 88,000 daltons, but with varying isoelectric points in the range of pH 6.5-7.0. Western blot analyses with antisera against the M1 and M2 proteins indicated that both subunit proteins are present in elevated quantities in the 600H cell line when compared to the wild-type V79 cell line. Southern blot analyses with genomic DNA from the series of stepwise-selected hydroxyurea-resistant cell lines leading to 600H showed that, in latter steps of selection, genomic sequences homologous to a mouse M1 cDNA have undergone a fivefold amplification. This was accompanied by a four- to eightfold increase in the single M1 homologous mRNA.
The paper makes six recommendations for changes in marketing practice which, most significantly, ... more The paper makes six recommendations for changes in marketing practice which, most significantly, add-up to need for the next big shift in marketing to be from ‘sense and provide to ‘co-create and guide for sustainability’. This follows the earlier big strategic shift in marketing from ‘make and sell’ to ‘sense and provide’. It notes that whilst some major companies are edging towards this ‘guide and co-create for sustainability’ approach the shift needs to be faster and more widespread if wellbeing is to be achieved for both people and the planet.
This paper has been written for Friends of the Earth by Dr Victoria Hurth, a lecturer in Marketing at Plymouth University, with over 13 years experience of working in marketing and sustainability. Her co-authors are Jules Peck of communication strategy and innovation consultancy Jericho Chambers; David Jackman, author of the first British Standard for Sustainable Development; and Dr Enrico Wensing from the George Mason University in the USA with expertise in psychology and sustainability.
The paper was produced to contribute to Friends of the Earth Big Ideas Project, which aims to identify 20-25 significant changes necessary to deliver wellbeing to people and the planet. It particularly contributes to the Big Ideas topic on consumption and identity.
We have constructed a cDNA library from the highly hydroxyurea-resistant hamster cell line 600H i... more We have constructed a cDNA library from the highly hydroxyurea-resistant hamster cell line 600H in which the activity of ribonucleotide reductase is elevated more than 80-fold. Using the technique of differential hybridization, we have isolated a number of cDNA clones from this library which are homologous to genomic DNA sequences amplified in the 600H cell line compared to the V79 parental line. One of these cDNA clones by sequence analysis was found to code for ornithine decarboxylase. This was confirmed by in vitro translation of poly(A+) RNA isolated by hybridization-selection followed by immunoprecipitation with antiserum specific for mouse ornithine decarboxylase. Genomic sequences homologous to the cDNA clone were shown to be sequentially amplified 6-20-fold in hamster cell lines selected stepwise for resistance to increasing concentrations of hydroxyurea. Genomic sequences homologous to a cDNA for the M2 subunit of ribonucleotide reductase were also amplified in these cell lines, and the degree of M2 sequence amplification corresponded to the degree of amplification of ornithine decarboxylase sequences, suggesting that the two genes had been co-amplified during the selection of the hydroxyurea-resistant phenotype.
Journal of Agricultural and Environmental Ethics, 2021
This essay proposes African-based ethical solutions to profound human problems and a working Afri... more This essay proposes African-based ethical solutions to profound human problems and a working African model to address those problems. The model promotes sustainability through advanced agroecological and information communication technologies. The essay’s first section reviews the ethical ground of that model in the work of the Senegalese scholar, Cheikh Anta Diop. The essay’s second section examines an applied African model for translating African ethical speculation into practice. Deeply immersed in European and African ethics, Godfrey Nzamujo developed the Songhaï Centers to solve the problem of rural poverty in seventeen African countries. Harnessing advanced technologies within a holistic agroecological ecosystem, Nzamujo’s villages furnish education spanning the fields of ethics, information communication technology, microbiology, international development, and mechanical, electrical, civil and biological engineering in a community-based and centered development enterprise. Th...
But marketing is here to stay. So how can it be transformed to be an agent of sustainability? As ... more But marketing is here to stay. So how can it be transformed to be an agent of sustainability? As part of Friends of the Earth’s Big Ideas Project we have been looking at this question. And we have concluded that marketing has to evolve from a paradigm of ‘sense and provide’ to a new paradigm, which we call ‘guide and co-create (for sustainability)’. Central to achieving this evolution is, we suggest, a ‘framework for evolved marketing’.
Abstract. Futures Consciousness refers to the capacity that a person has for understanding, antic... more Abstract. Futures Consciousness refers to the capacity that a person has for understanding, anticipating, and preparing for the future. Although the concept is widely used in the field of futures r...
The hydroxyurea-resistant Chinese hamster cell line 600H has been shown to have greatly elevated ... more The hydroxyurea-resistant Chinese hamster cell line 600H has been shown to have greatly elevated quantities of ribonucleotide reductase. This increase in enzyme activity is due to an increased level of both the M1 and M2 subunit activities. The M1 subunit has been purified from the 600H cell line and shown to consist of a series of six protein spots with apparent molecular weights of 88,000 daltons, but with varying isoelectric points in the range of pH 6.5-7.0. Western blot analyses with antisera against the M1 and M2 proteins indicated that both subunit proteins are present in elevated quantities in the 600H cell line when compared to the wild-type V79 cell line. Southern blot analyses with genomic DNA from the series of stepwise-selected hydroxyurea-resistant cell lines leading to 600H showed that, in latter steps of selection, genomic sequences homologous to a mouse M1 cDNA have undergone a fivefold amplification. This was accompanied by a four- to eightfold increase in the single M1 homologous mRNA.
The paper makes six recommendations for changes in marketing practice which, most significantly, ... more The paper makes six recommendations for changes in marketing practice which, most significantly, add-up to need for the next big shift in marketing to be from ‘sense and provide to ‘co-create and guide for sustainability’. This follows the earlier big strategic shift in marketing from ‘make and sell’ to ‘sense and provide’. It notes that whilst some major companies are edging towards this ‘guide and co-create for sustainability’ approach the shift needs to be faster and more widespread if wellbeing is to be achieved for both people and the planet.
This paper has been written for Friends of the Earth by Dr Victoria Hurth, a lecturer in Marketing at Plymouth University, with over 13 years experience of working in marketing and sustainability. Her co-authors are Jules Peck of communication strategy and innovation consultancy Jericho Chambers; David Jackman, author of the first British Standard for Sustainable Development; and Dr Enrico Wensing from the George Mason University in the USA with expertise in psychology and sustainability.
The paper was produced to contribute to Friends of the Earth Big Ideas Project, which aims to identify 20-25 significant changes necessary to deliver wellbeing to people and the planet. It particularly contributes to the Big Ideas topic on consumption and identity.
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This paper has been written for Friends of the Earth by Dr Victoria Hurth, a lecturer in Marketing at Plymouth University, with over 13 years experience of working in marketing and sustainability. Her co-authors are Jules Peck of communication strategy and innovation consultancy Jericho Chambers; David Jackman, author of the first British Standard for Sustainable Development; and Dr Enrico Wensing from the George Mason University in the USA with expertise in psychology and sustainability.
The paper was produced to contribute to Friends of the Earth Big Ideas Project, which aims to identify 20-25 significant changes necessary to deliver wellbeing to people and the planet. It particularly contributes to the Big Ideas topic on consumption and identity.
This paper has been written for Friends of the Earth by Dr Victoria Hurth, a lecturer in Marketing at Plymouth University, with over 13 years experience of working in marketing and sustainability. Her co-authors are Jules Peck of communication strategy and innovation consultancy Jericho Chambers; David Jackman, author of the first British Standard for Sustainable Development; and Dr Enrico Wensing from the George Mason University in the USA with expertise in psychology and sustainability.
The paper was produced to contribute to Friends of the Earth Big Ideas Project, which aims to identify 20-25 significant changes necessary to deliver wellbeing to people and the planet. It particularly contributes to the Big Ideas topic on consumption and identity.