A high-performance liquid chromatographic method for riboflavin determination in cheese samples was developed. Reverse phase conditions were used, i.e., a C-18 column isocratically eluted with an acetonitrile water mixture (20:80). A... more
A high-performance liquid chromatographic method for riboflavin determination in cheese samples was developed. Reverse phase conditions were used, i.e., a C-18 column isocratically eluted with an acetonitrile water mixture (20:80). A shift of the detection wavelength from 267 to 446 nm was proposed as a simple and very effective means for circumventing the problem of incomplete resolution of riboflavin from interfacing peaks. The detection limit of riboflavin was 2.5 ng, a value well above the level required for determining the riboflavin content in cheese samples. From both high- and low-fat cheese, the riboflavin recovery was the same (90.2%). The riboflavin content of several different Italian cheeses was determined.
Research Interests: Engineering, Chemical Engineering, Chemistry, Analytical Chemistry, Technology, and 12 moreFood Science, Glucose, Fruit, Fructose, High Pressure Liquid Chromatography, CHEMICAL SCIENCES, Food Sciences, Agricultural and Food Chemistry, CARBOHYDRATES, Sugar Alcohols, Biochemistry and cell biology, and Pharmacology and pharmaceutical sciences
Analyse des carotenoides extraits de l'insaponifiable de l'huile d'olive par une methode simple et rapide de chromatographie HPLC en phase inverse non aqueuse. La luteine et le β-carotene sont caracteristiques des huiles... more
Analyse des carotenoides extraits de l'insaponifiable de l'huile d'olive par une methode simple et rapide de chromatographie HPLC en phase inverse non aqueuse. La luteine et le β-carotene sont caracteristiques des huiles d'olive, leurs concentrations variant suivant la qualite de l'huile
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Research Interests: Humanities and Aloe
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Abstract A high-performance liquid chromatographic method developed for the determination of both okadaic acid (OA) and free fatty acids (FFA) was used for analysing mussel samples collected in the Gulf of Trieste. OA and FFA extracted... more
Abstract A high-performance liquid chromatographic method developed for the determination of both okadaic acid (OA) and free fatty acids (FFA) was used for analysing mussel samples collected in the Gulf of Trieste. OA and FFA extracted from mussel ...
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Research Interests: Engineering, Chemical Engineering, Analytical Chemistry, Technology, Food Science, and 11 moreGlucose, Fruit, Fructose, High Pressure Liquid Chromatography, CHEMICAL SCIENCES, Food Sciences, Agricultural and Food Chemistry, CARBOHYDRATES, Sugar Alcohols, Biochemistry and cell biology, and Pharmacology and pharmaceutical sciences
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Retinol (vitamin A1) and dehydroretinol (vitamin A2) geometric isomers, obtained in photosynthetic mixtures or from natural samples, were simultaneously fractionated by means of isocratic normal phase high-performance liquid... more
Retinol (vitamin A1) and dehydroretinol (vitamin A2) geometric isomers, obtained in photosynthetic mixtures or from natural samples, were simultaneously fractionated by means of isocratic normal phase high-performance liquid chromatography. The use of low percentages of isopropanol (0.4-1.1%) as a modifier in hexane was studied, and the resolution of both pairs of retinols (9-cis/all-trans and 13-cis/11-cis) whose baseline separation had not previously been achieved was obtained. The proposed chromatographic method therefore allows the complete separation of all six of the most commonly occurring retinols in natural samples, including the above four mono cis- retinols plus the two 9,13- and 11,13-di-cis- retinols . Photoisomerization of retinal and of retinol in different solvent systems and during the extraction process was also studied, before analyzing the unsaponifiable from fresh and marine water fish, which constitute rich natural sources of both vitamins A1 and A2. Vitamin A2 was found to be present in different relative percentages depending on the analytical matrix. Four cis isomers (9-cis-, 11-cis-, 13-cis- and 9,13-di-cis-retinol or corresponding dehydroretinols ) were found to occur naturally together with the main all-trans form, confirming the need to separate geometric isomers in every dosage of vitamin A-containing compounds.
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Résumé/Abstract The recently published 2001/18/EC Directive on deliberate release into the environment of Genetically Modified Organisms (GMOs) provides a very important legislative framework for regulation of the GMOs issue in the... more
Résumé/Abstract The recently published 2001/18/EC Directive on deliberate release into the environment of Genetically Modified Organisms (GMOs) provides a very important legislative framework for regulation of the GMOs issue in the European Union in the ...
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Research Interests: Engineering, Chemical Engineering, Analytical Chemistry, Technology, Chromatography, and 17 moreMass Spectrometry, Carotenoids, Quantitative analysis, Food Analysis, Oils, Vitamin A, Vitamin E, Gas Chromatography, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, CHEMICAL SCIENCES, Liquid Chromatography, Fatty Acid, Cod Liver Oil, Quantitative Analysis, High Sensitivity, and Butter
Research Interests: Engineering, Chemical Engineering, Analytical Chemistry, Technology, Chromatography, and 17 moreMass Spectrometry, Carotenoids, Quantitative analysis, Food Analysis, Oils, Vitamin A, Vitamin E, Gas Chromatography, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, CHEMICAL SCIENCES, Liquid Chromatography, Fatty Acid, Cod Liver Oil, Quantitative Analysis, High Sensitivity, and Butter
A study of mobile phases for the improved high-performance liquid chromatographic resolution of retinal, 3-dehydroretinal, retinol and 3-dehydroretinol stereoisomers is described. By using 1-octanol as a phase modifier in n-hexane, the... more
A study of mobile phases for the improved high-performance liquid chromatographic resolution of retinal, 3-dehydroretinal, retinol and 3-dehydroretinol stereoisomers is described. By using 1-octanol as a phase modifier in n-hexane, the simultaneous separation of a complex mixture of vitamin A1 and A2 isomers was satisfactorily achieved, while the separation of the corresponding aldehydes required a ternary mixture of 2-propanol, dioxane and n-hexane. All peaks were spectroscopically characterized by recording their UV spectra, which are reported together with comparative tables of the absorbance maxima of the stereoisomers.
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Retinol (vitamin A1) and dehydroretinol (vitamin A2) geometric isomers, obtained in photosynthetic mixtures or from natural samples, were simultaneously fractionated by means of isocratic normal phase high-performance liquid... more
Retinol (vitamin A1) and dehydroretinol (vitamin A2) geometric isomers, obtained in photosynthetic mixtures or from natural samples, were simultaneously fractionated by means of isocratic normal phase high-performance liquid chromatography. The use of low percentages of isopropanol (0.4-1.1%) as a modifier in hexane was studied, and the resolution of both pairs of retinols (9-cis/all-trans and 13-cis/11-cis) whose baseline separation had not previously been achieved was obtained. The proposed chromatographic method therefore allows the complete separation of all six of the most commonly occurring retinols in natural samples, including the above four mono cis- retinols plus the two 9,13- and 11,13-di-cis- retinols . Photoisomerization of retinal and of retinol in different solvent systems and during the extraction process was also studied, before analyzing the unsaponifiable from fresh and marine water fish, which constitute rich natural sources of both vitamins A1 and A2. Vitamin A2 was found to be present in different relative percentages depending on the analytical matrix. Four cis isomers (9-cis-, 11-cis-, 13-cis- and 9,13-di-cis-retinol or corresponding dehydroretinols ) were found to occur naturally together with the main all-trans form, confirming the need to separate geometric isomers in every dosage of vitamin A-containing compounds.