<p>Relative abundances of taxa are reported in percentage. “Unidentified fungi” comprehends... more <p>Relative abundances of taxa are reported in percentage. “Unidentified fungi” comprehends Fungi sp. and fungi with no blast hit. Taxa accounting for <0.1% of reads are grouped as “other”.</p
<p>Significant differences among sites were calculated with ANOVA and Duncan’s new multiple... more <p>Significant differences among sites were calculated with ANOVA and Duncan’s new multiple range test and are indicated by different letters a-c (p-value < 0.05).</p
Journal of biological regulators and homeostatic agents
Two hepatotrophic protein factors (HTF) were partially purified from the serum-free conditioned m... more Two hepatotrophic protein factors (HTF) were partially purified from the serum-free conditioned medium of the embryo rat fibroblastic cell line FRL. HTF induced cell adherence to the culture plate, promoted life maintenance, scattering and enhanced 3H-Thymidine uptake into DNA and cell proliferation in two sensitive murine non-transformed, epithelial hepatocytic cell lines (C6 and C2.8). In this bio-assay, cells were plated at low density in Dulbecco's Modified Eagle's Medium, in the absence of serum and other exogenous growth factors. HTF did not synergize with epidermal growth factor. Biological activity was associated to two major acidic proteins: a non-heparin-binding protein (M(r) approximately 70 kDa, pI 4.6-4.5) and a heparin-binding protein (M(r) approximately 200 KDa, pI 4.3-4.1).
Shiga-toxin-producing Escherichia coli (STEC) represents a significant cause of foodborne disease... more Shiga-toxin-producing Escherichia coli (STEC) represents a significant cause of foodborne disease. In the last years, an increasing number of STEC infections associated with the consumption of raw and pasteurized milk cheese have been reported, contributing to raise the public awareness. The aim of this study is to evaluate the main genomic features of STEC strains isolated from a semi-hard raw milk cheese, focusing on their pathogenic potential. The analysis of 75 cheese samples collected during the period between April 2019 and January 2020 led to the isolation of seven strains from four stx-positive enrichment. The genome investigation evidenced the persistence of two serotypes, O174:H2 and O116:H48. All strains carried at least one stx gene and were negative for eae gene. The virulence gene pattern was homogeneous among the serogroup/ST and included adherence factors (lpfA, iha, ompT, papC, saa, sab, hra, and hes), enterohemolysin (ehxA), serum resistance (iss, tra), cytotoxin-e...
<p>Relative abundances of taxa are reported in percentage. “Unidentified fungi” comprehends... more <p>Relative abundances of taxa are reported in percentage. “Unidentified fungi” comprehends Fungi sp. and fungi with no blast hit. Taxa accounting for <0.1% of reads are grouped as “other”.</p
<p>Significant differences among sites were calculated with ANOVA and Duncan’s new multiple... more <p>Significant differences among sites were calculated with ANOVA and Duncan’s new multiple range test and are indicated by different letters a-c (p-value < 0.05).</p
Journal of biological regulators and homeostatic agents
Two hepatotrophic protein factors (HTF) were partially purified from the serum-free conditioned m... more Two hepatotrophic protein factors (HTF) were partially purified from the serum-free conditioned medium of the embryo rat fibroblastic cell line FRL. HTF induced cell adherence to the culture plate, promoted life maintenance, scattering and enhanced 3H-Thymidine uptake into DNA and cell proliferation in two sensitive murine non-transformed, epithelial hepatocytic cell lines (C6 and C2.8). In this bio-assay, cells were plated at low density in Dulbecco's Modified Eagle's Medium, in the absence of serum and other exogenous growth factors. HTF did not synergize with epidermal growth factor. Biological activity was associated to two major acidic proteins: a non-heparin-binding protein (M(r) approximately 70 kDa, pI 4.6-4.5) and a heparin-binding protein (M(r) approximately 200 KDa, pI 4.3-4.1).
Shiga-toxin-producing Escherichia coli (STEC) represents a significant cause of foodborne disease... more Shiga-toxin-producing Escherichia coli (STEC) represents a significant cause of foodborne disease. In the last years, an increasing number of STEC infections associated with the consumption of raw and pasteurized milk cheese have been reported, contributing to raise the public awareness. The aim of this study is to evaluate the main genomic features of STEC strains isolated from a semi-hard raw milk cheese, focusing on their pathogenic potential. The analysis of 75 cheese samples collected during the period between April 2019 and January 2020 led to the isolation of seven strains from four stx-positive enrichment. The genome investigation evidenced the persistence of two serotypes, O174:H2 and O116:H48. All strains carried at least one stx gene and were negative for eae gene. The virulence gene pattern was homogeneous among the serogroup/ST and included adherence factors (lpfA, iha, ompT, papC, saa, sab, hra, and hes), enterohemolysin (ehxA), serum resistance (iss, tra), cytotoxin-e...
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Papers by Francesca Borney