Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 ... more Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 Bcr-Abl) that contains NH2-terminal sequences from Bcr fused to COOH-terminal sequences from Abl. In a yeast two-hybrid screen, we have identified TSG101 as a binding partner for Bcr. Because TSG101 is a subunit of the mammalian endosomal sorting complex required for transport (ESCRT), which regulates protein sorting during endosomal trafficking, this association suggests that Bcr may have a related cellular function. The docking site for TSG101 has been mapped to the COOH terminus of Bcr, indicating that this interaction may be disrupted in CML. Overexpression studies with full-length TSG101 and Bcr reveal that this interaction can be recapitulated in mammalian cells. The association can also be observed between natively expressed proteins in a panel of hematopoietic and nonhematopoietic cell lines, where a second subunit of the ESCRT complex, vacuolar sorting protein 28 (Vps28), was al...
Multiple technology based tools have been used to enhance skill development in allied health educ... more Multiple technology based tools have been used to enhance skill development in allied health education, which now includes virtual learning environments. The purpose of this study was to explore whether, and how, this latest instructional technology is being adapted in allied health education. An online survey was circulated to all Association of Schools of Allied Health Professions (ASAHP) member institutions and focused on three broad areas of virtual learning environments: the uses of, the perceived pros and cons of, and the outcomes of utilizing them. Results show 40% (17 of 42) of the respondent use some form of the technology. The use of virtual learning technology in other healthcare professions (e.g., medicine) demonstrates the potential benefits to allied health education.
The oncogenic fusion protein p210 Bcr-Abl is causally associated with virtually all cases of chro... more The oncogenic fusion protein p210 Bcr-Abl is causally associated with virtually all cases of chronic myelogenous leukemia. The wild-type Bcr product has several recognizable structural and functional motifs including a domain that contains guanine nucleotide exchange activity for Rho family GTPases (DH/PH domain). Although this domain is retained within p210 Bcr-Abl, it has no known signaling activities in vivo. Here we report that a fragment of Bcr that encodes the isolated DH/PH domain is a potent activator of the NF-kappaB transcription factor. Within the context of full length Bcr, this activity is regulated by proximal flanking sequences that suppress the DH/PH domain encoded guanine nucleotide exchange activity. NF-kappaB activation by Bcr is not mediated by nuclear translocation, but rather by p38 mitogen-activated protein kinase (MAPK)-dependent modification of the RelA/p65 transactivation domain. Although we were able to demonstrate that Bcr can function as an exchange fact...
and is one of only eight of its kind currently funded by the NCI. This program, which has been co... more and is one of only eight of its kind currently funded by the NCI. This program, which has been continuously funded by the NCI for 40 years, provides a unique eight-week research experience for New Jersey Medical School's first and second-year medical students as well as undergraduate students enrolled in our combined BS/MD seven-year program. This year 18 medical students and 3 undergraduates participated in biomedical research activities in both laboratory and clinical settings at either the NJMS-UH Cancer Center, or the broader cancer center research community on the Newark Campus, while developing a close working relationship with their faculty mentors. The administration of the summer program is focused at the Cancer Center where it is a part of the center's broader training mission in cancer education for both PhD and MD scientists. The Program Directors, Drs. Ozer and Mahon, and the Program Coordinator, Ms. Lorie-Anne Philips, expanded the program to include student at...
and is one of only eight of its kind currently funded by the NCI (competitive renewal in 2010). T... more and is one of only eight of its kind currently funded by the NCI (competitive renewal in 2010). This program, which has been continuously funded by the NCI for 41 years, provides a unique eight-week research experience for New Jersey Medical School's first and second-year medical students as well as undergraduate students enrolled in our combined BS/MD seven-year program. This year 21 medical students and one undergraduate participated in biomedical research activities in both laboratory and clinical settings at either the NJMS-UH Cancer Center, or the broader cancer center research community on the Newark Campus, while developing a close working relationship with their faculty mentors. The administration of the summer program is focused at the Cancer Center where it is a part of the center's broader training mission in cancer education for both PhD and MD scientists. The Program Directors, Drs. Ozer and Mahon, and the Program Coordinator, Ms. Lorie-Anne Philips, expanded th...
Comprehensive understanding of the cellular mechanisms utilized by luteal cells in response to ex... more Comprehensive understanding of the cellular mechanisms utilized by luteal cells in response to extracellular hormonal signals resulting in the normal synthesis and secretion of their steroid and peptide products has yet to be achieved. Previous studies have established that cAMP functions as a second messenger in mediating gonadotropin stimulated luteal progesterone secretion. Classically, increased intracellular concentrations of cAMP result in activation of protein kinase A (PKA), which in turn phosphorylates gene regulatory transcription factors. Recent studies demonstrate that non-PKA mediated actions of cAMP exist, yet the mechanisms are not well understood. In addition to gonadotropic hormones, such growth factors as insulin, insulin-like growth factor 1, and epidermal growth factor have been shown to modulate luteal steroid hormone synthesis and steroidogenic enzyme expression as either independent effects or via amplification or modulation of the action of gonadotropic hormones or cAMP. Thus, mechanisms independent of cAMP and also downstream to cAMP that do not involve PKA are likely to be important in steroidogenesis in mammalian cells. The present studies were performed to help define the cellular mediators involved in cAMP-stimulated progesterone expression. Our data demonstrate that, in an in vitro steroidogenic cell model, 1) cAMP-stimulated progesterone occurs in a manner that is independent of PKA, 2) neither phosphatidylinositol-3-kinase nor mitogen-activated protein kinase are involved in PKA-independent cAMP-stimulated progesterone production, 3) tyrosine kinase activity does mediate cAMP-stimulated progesterone production, and 4) cAMP directly activates the Ras protein. These data suggest novel mediators of cAMP-stimulated progesterone production.
Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 ... more Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 Bcr-Abl) that contains NH2-terminal sequences from Bcr fused to COOH- terminal sequences from Abl. In a yeast two-hybrid screen, we have identified TSG101 as a binding partner for Bcr. Because TSG101 is a subunit of the mammalian endosomal sorting complex required for transport (ESCRT), which regulates protein
Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 ... more Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 Bcr-Abl) that contains NH2-terminal sequences from Bcr fused to COOH-terminal sequences from Abl. In a yeast two-hybrid screen, we have identified TSG101 as a binding partner for Bcr. Because TSG101 is a subunit of the mammalian endosomal sorting complex required for transport (ESCRT), which regulates protein sorting during endosomal trafficking, this association suggests that Bcr may have a related cellular function. The docking site for TSG101 has been mapped to the COOH terminus of Bcr, indicating that this interaction may be disrupted in CML. Overexpression studies with full-length TSG101 and Bcr reveal that this interaction can be recapitulated in mammalian cells. The association can also be observed between natively expressed proteins in a panel of hematopoietic and nonhematopoietic cell lines, where a second subunit of the ESCRT complex, vacuolar sorting protein 28 (Vps28), was al...
Multiple technology based tools have been used to enhance skill development in allied health educ... more Multiple technology based tools have been used to enhance skill development in allied health education, which now includes virtual learning environments. The purpose of this study was to explore whether, and how, this latest instructional technology is being adapted in allied health education. An online survey was circulated to all Association of Schools of Allied Health Professions (ASAHP) member institutions and focused on three broad areas of virtual learning environments: the uses of, the perceived pros and cons of, and the outcomes of utilizing them. Results show 40% (17 of 42) of the respondent use some form of the technology. The use of virtual learning technology in other healthcare professions (e.g., medicine) demonstrates the potential benefits to allied health education.
The oncogenic fusion protein p210 Bcr-Abl is causally associated with virtually all cases of chro... more The oncogenic fusion protein p210 Bcr-Abl is causally associated with virtually all cases of chronic myelogenous leukemia. The wild-type Bcr product has several recognizable structural and functional motifs including a domain that contains guanine nucleotide exchange activity for Rho family GTPases (DH/PH domain). Although this domain is retained within p210 Bcr-Abl, it has no known signaling activities in vivo. Here we report that a fragment of Bcr that encodes the isolated DH/PH domain is a potent activator of the NF-kappaB transcription factor. Within the context of full length Bcr, this activity is regulated by proximal flanking sequences that suppress the DH/PH domain encoded guanine nucleotide exchange activity. NF-kappaB activation by Bcr is not mediated by nuclear translocation, but rather by p38 mitogen-activated protein kinase (MAPK)-dependent modification of the RelA/p65 transactivation domain. Although we were able to demonstrate that Bcr can function as an exchange fact...
and is one of only eight of its kind currently funded by the NCI. This program, which has been co... more and is one of only eight of its kind currently funded by the NCI. This program, which has been continuously funded by the NCI for 40 years, provides a unique eight-week research experience for New Jersey Medical School's first and second-year medical students as well as undergraduate students enrolled in our combined BS/MD seven-year program. This year 18 medical students and 3 undergraduates participated in biomedical research activities in both laboratory and clinical settings at either the NJMS-UH Cancer Center, or the broader cancer center research community on the Newark Campus, while developing a close working relationship with their faculty mentors. The administration of the summer program is focused at the Cancer Center where it is a part of the center's broader training mission in cancer education for both PhD and MD scientists. The Program Directors, Drs. Ozer and Mahon, and the Program Coordinator, Ms. Lorie-Anne Philips, expanded the program to include student at...
and is one of only eight of its kind currently funded by the NCI (competitive renewal in 2010). T... more and is one of only eight of its kind currently funded by the NCI (competitive renewal in 2010). This program, which has been continuously funded by the NCI for 41 years, provides a unique eight-week research experience for New Jersey Medical School's first and second-year medical students as well as undergraduate students enrolled in our combined BS/MD seven-year program. This year 21 medical students and one undergraduate participated in biomedical research activities in both laboratory and clinical settings at either the NJMS-UH Cancer Center, or the broader cancer center research community on the Newark Campus, while developing a close working relationship with their faculty mentors. The administration of the summer program is focused at the Cancer Center where it is a part of the center's broader training mission in cancer education for both PhD and MD scientists. The Program Directors, Drs. Ozer and Mahon, and the Program Coordinator, Ms. Lorie-Anne Philips, expanded th...
Comprehensive understanding of the cellular mechanisms utilized by luteal cells in response to ex... more Comprehensive understanding of the cellular mechanisms utilized by luteal cells in response to extracellular hormonal signals resulting in the normal synthesis and secretion of their steroid and peptide products has yet to be achieved. Previous studies have established that cAMP functions as a second messenger in mediating gonadotropin stimulated luteal progesterone secretion. Classically, increased intracellular concentrations of cAMP result in activation of protein kinase A (PKA), which in turn phosphorylates gene regulatory transcription factors. Recent studies demonstrate that non-PKA mediated actions of cAMP exist, yet the mechanisms are not well understood. In addition to gonadotropic hormones, such growth factors as insulin, insulin-like growth factor 1, and epidermal growth factor have been shown to modulate luteal steroid hormone synthesis and steroidogenic enzyme expression as either independent effects or via amplification or modulation of the action of gonadotropic hormones or cAMP. Thus, mechanisms independent of cAMP and also downstream to cAMP that do not involve PKA are likely to be important in steroidogenesis in mammalian cells. The present studies were performed to help define the cellular mediators involved in cAMP-stimulated progesterone expression. Our data demonstrate that, in an in vitro steroidogenic cell model, 1) cAMP-stimulated progesterone occurs in a manner that is independent of PKA, 2) neither phosphatidylinositol-3-kinase nor mitogen-activated protein kinase are involved in PKA-independent cAMP-stimulated progesterone production, 3) tyrosine kinase activity does mediate cAMP-stimulated progesterone production, and 4) cAMP directly activates the Ras protein. These data suggest novel mediators of cAMP-stimulated progesterone production.
Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 ... more Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 Bcr-Abl) that contains NH2-terminal sequences from Bcr fused to COOH- terminal sequences from Abl. In a yeast two-hybrid screen, we have identified TSG101 as a binding partner for Bcr. Because TSG101 is a subunit of the mammalian endosomal sorting complex required for transport (ESCRT), which regulates protein
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