Upon fertilization, sea urchin eggs (Stronglyocentrotus pupuratus) release a protease into the su... more Upon fertilization, sea urchin eggs (Stronglyocentrotus pupuratus) release a protease into the surrounding sea water. This protease is in a particulate form which can be solubilized. The soluble form was purified by affinity chromatography on columns of immobilized soybean trypsin inhibitor. The purified enzyme is similar to bovine trypsin both in molecular weight (22500) and in susceptibility to inhibitors such as diisopropyl phosphofluoridate and soybean trypsin inhibitor. In contrast, extracts of unfertilized eggs appear to contain an inactive form of the enzyme which can be activated by dialysis at pH 4.6. The enzyme, as purified from extracts activated in this manner, was similar in its properties to that from fertilized eggs.
A study was conducted to evaluate the effects of feeding various sources of dietary lipid on weig... more A study was conducted to evaluate the effects of feeding various sources of dietary lipid on weight gain, feed conversion, survival and fatty acid composition of juvenile Penaeus vannamei. Seven semi-purified diets (35% protein and 3400 kcal of metabolizable energy kg−1) containing defatted, freeze-dried shrimp meal, 1.0% soybean lecithin and 0.5% cholesterol were supplemented with 6.5% of either stearic acid, coconut, safflower, corn, soybean, linseed or menhaden fish oils. Each diet was fed to shrimp (1.00 ± 0.03 g average weight) in four replicate aquaria four times daily for 10 weeks. Weight gain, feed conversion and survival were best for shrimp fed the diet containing menhaden fish oil. Shrimp fed the linseed oil diet had the second highest weight gain, followed by shrimp on soybean oil, corn oil, stearic acid, coconut oil and safflower oil diets, respectively. Feed conversion values were a reflection of weight gain. Results of this study show that both n-6 and n-3 fatty acids...
Fatty acid profiles were obtained for macadamia nut oil and several other cooking oils. Macadamia... more Fatty acid profiles were obtained for macadamia nut oil and several other cooking oils. Macadamia nut oil contained the highest level (79%) of monounsaturated fatty acids. Macadamia nut oil is low (4%) in the omega-6 fatty acid 18:2n-6 and saturated fatty acids. Fatty acid profiles for popcorn cooked in several oils were obtained for comparison because of public awareness and concern over high levels of saturated fat found in popcorn sold in theaters. The nutritional implications of using macadamia nut oil are discussed.
Tamaru, CS, Ako, H., Sato, VT and Weidenbach, RP (2008) Advances in the Culture of Rotifers for u... more Tamaru, CS, Ako, H., Sato, VT and Weidenbach, RP (2008) Advances in the Culture of Rotifers for use in Rearing Marine Ornamental Fish, in Marine Ornamental Species: Collection, Culture & Conservation (eds JC Cato and CL Brown), Blackwell Publishing ...
The amino acid gamma-carboxyglutamic acid (Gla) has been previously detected in the vitamin K-req... more The amino acid gamma-carboxyglutamic acid (Gla) has been previously detected in the vitamin K-requiring blood clotting factors, proteins of calcified vertebrate tissue, renal tissue, plasma protein C, ectopic calcifications, and calcium-containing renal calculi. This paper reports the presence of Gla in the EDTA-soluble, non-dialyzable material recovered from human pediatric bladder stones containing calcium salts. In bladder stones composed of calcium oxalate, uric acid and ammonium acid urate, 73 Gla residues per 1,000 amino acid residues were detected. Bladder stones composed of calcium oxalate, uric acid, ammonium acid urate, and hydroxyapatite contained 48 Gla residues per 1,000 amino acid residues present. No Gla was detected in the predominantly magnesium ammonium phosphate (struvite) bladder stones. These results with human bladder stones from children under 10 years of age are consistent with the observations from adult patients in which Gla was detected in the calcium-cont...
Omega-3 fatty acids, the most potent of which are found in seafood, are of interest because of th... more Omega-3 fatty acids, the most potent of which are found in seafood, are of interest because of their effects on cardiovascular and inflammatory diseases and their possible effects on cancer. However, consumers in Hawaii wishing to increase their dietary omega-3 to omega-6 fatty acid ratio are faced with the difficulty that several types of seafood popular in Hawaii and aquacultured seafood new in the marketplace have unknown omega-3 fatty acid levels. The purpose of this work is to determine omega-3 fatty acid levels of selected seafood and fish oil capsules. Several seafoods and some over-the-counter fish oil capsules were sampled and analyzed. Aku eggs, aquacultured hamachi (yellowtail jack from Japan), one sample of turbot, and EPA Plus, Promega, and Omega-3 Super EPA capsules were found to contain high levels of omega-3 fatty acids. Levels were comparable to those in the fatty fishes such as salmon and mackerel. Butterfish, mahimahi eggs; other fish oil capsules (ProEPA and Omeg...
A fibrinolysis enzyme activator was isolated from commercial bromelain. This enzyme preparation i... more A fibrinolysis enzyme activator was isolated from commercial bromelain. This enzyme preparation is used medicinally. A by-product of bromelain production, the acetone still residue, was used as a starting material to develop an isolation scheme which began with reversed phase chromatography, was followed by ion exchange chromatography, and ended with ultrafiltration. It had polarity and ion exchange properties similar to those of a neutral amino acid. The active substance has no intrinsic fibrinolytic activity but appears to enhance the activity of one of the fibrinolytic enzymes. The presence of the fibrinolysis enzyme activator in commercial bromelain may explain some of the physiological and clinical effects observed following the oral administration of the enzyme preparation.
... investigations, in Hawaii, on growout of angelfish, Pterophyllum scalare, and gold-fish, Cara... more ... investigations, in Hawaii, on growout of angelfish, Pterophyllum scalare, and gold-fish, Carassius auratus, using feeds developed for the culture of carnivorous marine fish species have resulted in significant improvements in growth and cost savings for their culture (Ako, et al ...
Page 1. CARCASS AND MEAT QUALITY CHARACTERISTICS OF FORAGE-BASED BEEF GK FUKUMOTO', YS KIM*,... more Page 1. CARCASS AND MEAT QUALITY CHARACTERISTICS OF FORAGE-BASED BEEF GK FUKUMOTO', YS KIM*, KH KIMz and H. AK03 'Cooperative Extension Service Kealakekua, HI 96750 *Department of Animal Sciences ...
Upon fertilization, sea urchin eggs (Stronglyocentrotus pupuratus) release a protease into the su... more Upon fertilization, sea urchin eggs (Stronglyocentrotus pupuratus) release a protease into the surrounding sea water. This protease is in a particulate form which can be solubilized. The soluble form was purified by affinity chromatography on columns of immobilized soybean trypsin inhibitor. The purified enzyme is similar to bovine trypsin both in molecular weight (22500) and in susceptibility to inhibitors such as diisopropyl phosphofluoridate and soybean trypsin inhibitor. In contrast, extracts of unfertilized eggs appear to contain an inactive form of the enzyme which can be activated by dialysis at pH 4.6. The enzyme, as purified from extracts activated in this manner, was similar in its properties to that from fertilized eggs.
A study was conducted to evaluate the effects of feeding various sources of dietary lipid on weig... more A study was conducted to evaluate the effects of feeding various sources of dietary lipid on weight gain, feed conversion, survival and fatty acid composition of juvenile Penaeus vannamei. Seven semi-purified diets (35% protein and 3400 kcal of metabolizable energy kg−1) containing defatted, freeze-dried shrimp meal, 1.0% soybean lecithin and 0.5% cholesterol were supplemented with 6.5% of either stearic acid, coconut, safflower, corn, soybean, linseed or menhaden fish oils. Each diet was fed to shrimp (1.00 ± 0.03 g average weight) in four replicate aquaria four times daily for 10 weeks. Weight gain, feed conversion and survival were best for shrimp fed the diet containing menhaden fish oil. Shrimp fed the linseed oil diet had the second highest weight gain, followed by shrimp on soybean oil, corn oil, stearic acid, coconut oil and safflower oil diets, respectively. Feed conversion values were a reflection of weight gain. Results of this study show that both n-6 and n-3 fatty acids...
Fatty acid profiles were obtained for macadamia nut oil and several other cooking oils. Macadamia... more Fatty acid profiles were obtained for macadamia nut oil and several other cooking oils. Macadamia nut oil contained the highest level (79%) of monounsaturated fatty acids. Macadamia nut oil is low (4%) in the omega-6 fatty acid 18:2n-6 and saturated fatty acids. Fatty acid profiles for popcorn cooked in several oils were obtained for comparison because of public awareness and concern over high levels of saturated fat found in popcorn sold in theaters. The nutritional implications of using macadamia nut oil are discussed.
Tamaru, CS, Ako, H., Sato, VT and Weidenbach, RP (2008) Advances in the Culture of Rotifers for u... more Tamaru, CS, Ako, H., Sato, VT and Weidenbach, RP (2008) Advances in the Culture of Rotifers for use in Rearing Marine Ornamental Fish, in Marine Ornamental Species: Collection, Culture & Conservation (eds JC Cato and CL Brown), Blackwell Publishing ...
The amino acid gamma-carboxyglutamic acid (Gla) has been previously detected in the vitamin K-req... more The amino acid gamma-carboxyglutamic acid (Gla) has been previously detected in the vitamin K-requiring blood clotting factors, proteins of calcified vertebrate tissue, renal tissue, plasma protein C, ectopic calcifications, and calcium-containing renal calculi. This paper reports the presence of Gla in the EDTA-soluble, non-dialyzable material recovered from human pediatric bladder stones containing calcium salts. In bladder stones composed of calcium oxalate, uric acid and ammonium acid urate, 73 Gla residues per 1,000 amino acid residues were detected. Bladder stones composed of calcium oxalate, uric acid, ammonium acid urate, and hydroxyapatite contained 48 Gla residues per 1,000 amino acid residues present. No Gla was detected in the predominantly magnesium ammonium phosphate (struvite) bladder stones. These results with human bladder stones from children under 10 years of age are consistent with the observations from adult patients in which Gla was detected in the calcium-cont...
Omega-3 fatty acids, the most potent of which are found in seafood, are of interest because of th... more Omega-3 fatty acids, the most potent of which are found in seafood, are of interest because of their effects on cardiovascular and inflammatory diseases and their possible effects on cancer. However, consumers in Hawaii wishing to increase their dietary omega-3 to omega-6 fatty acid ratio are faced with the difficulty that several types of seafood popular in Hawaii and aquacultured seafood new in the marketplace have unknown omega-3 fatty acid levels. The purpose of this work is to determine omega-3 fatty acid levels of selected seafood and fish oil capsules. Several seafoods and some over-the-counter fish oil capsules were sampled and analyzed. Aku eggs, aquacultured hamachi (yellowtail jack from Japan), one sample of turbot, and EPA Plus, Promega, and Omega-3 Super EPA capsules were found to contain high levels of omega-3 fatty acids. Levels were comparable to those in the fatty fishes such as salmon and mackerel. Butterfish, mahimahi eggs; other fish oil capsules (ProEPA and Omeg...
A fibrinolysis enzyme activator was isolated from commercial bromelain. This enzyme preparation i... more A fibrinolysis enzyme activator was isolated from commercial bromelain. This enzyme preparation is used medicinally. A by-product of bromelain production, the acetone still residue, was used as a starting material to develop an isolation scheme which began with reversed phase chromatography, was followed by ion exchange chromatography, and ended with ultrafiltration. It had polarity and ion exchange properties similar to those of a neutral amino acid. The active substance has no intrinsic fibrinolytic activity but appears to enhance the activity of one of the fibrinolytic enzymes. The presence of the fibrinolysis enzyme activator in commercial bromelain may explain some of the physiological and clinical effects observed following the oral administration of the enzyme preparation.
... investigations, in Hawaii, on growout of angelfish, Pterophyllum scalare, and gold-fish, Cara... more ... investigations, in Hawaii, on growout of angelfish, Pterophyllum scalare, and gold-fish, Carassius auratus, using feeds developed for the culture of carnivorous marine fish species have resulted in significant improvements in growth and cost savings for their culture (Ako, et al ...
Page 1. CARCASS AND MEAT QUALITY CHARACTERISTICS OF FORAGE-BASED BEEF GK FUKUMOTO', YS KIM*,... more Page 1. CARCASS AND MEAT QUALITY CHARACTERISTICS OF FORAGE-BASED BEEF GK FUKUMOTO', YS KIM*, KH KIMz and H. AK03 'Cooperative Extension Service Kealakekua, HI 96750 *Department of Animal Sciences ...
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