In contemporary literature there are data about significant role of free radical oxidation in the... more In contemporary literature there are data about significant role of free radical oxidation in the pathogenesis of obesity. In this relation application of endogenous as well as exogenous antioxidants for the purpose of excess weight correction has generated increasing interest in scientific circles. Numerous researches showed natural antioxidants to be effective in correction of elevated blood cholesterol, triglycerides, LDL levels. In this relation the purpose of our research was to establish effectiveness of green tea catechines and citrus extract on lipid metabolism disorder and excess body weight during experimental alimentary obesity. Experiment was conducted on rats kept on high-calorie diet for 7 weeks. Simultaneously animals from III and IV group had been administered catechines and citrus extract at the doses of 15 mg and 30 mg respectively in addition to the high-calorie diet during last 4 weeks. The experiment established corrective effect of abovementioned preparations o...
The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular... more The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential ofT. patulacompounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralizati...
Background: Recovery of dental arch defects, related to insertion of a foreign bodies into the or... more Background: Recovery of dental arch defects, related to insertion of a foreign bodies into the oral cavity, and the impact of mechanical pressure, allergic-toxic effects, and the related inflammatory and degenerative processes is one of the most urgent problems in dentistry.The purpose of the study was to evaluate the efficacy of Georgian bean extract tincture as mouthwash for patients using a lever system to correct deformities of the jaw and teeth.Methods: Twenty-eight patients (aged 12-25 years) with deformities of the jaw and teeth were studied. Patients were examined 6-8 months after the bracket system was installed, and before and after the use of tincture mouthwash for 10 days. Patients were randomly divided into 2 groups: the control group for the mouthwash used standard tincture ("Octenidol" Schulke and Mayr -Germany); the patients of the experimental group used tincture made from the Georgian legume crop extracts (GLCE). Before and after the use of both types of ...
Summer savory (Satureja hortensisL.,Lamiaceae) is used in several regions of the world as a spice... more Summer savory (Satureja hortensisL.,Lamiaceae) is used in several regions of the world as a spice and folk medicine. Anti-inflammatory and cytoprotective effects ofS. hortensisand of its rosmarinic acid-rich phenolic fraction have been demonstrated in animal trials. However, previous studies of rosmarinic acid in cell models have yielded controversial results. In this study, we investigated the effects of summer savory extracts on H2O2-challenged human lymphoblastoid Jurkat T cells. LC-MS analysis confirmed the presence of rosmarinic acid and flavonoids such as hesperidin and naringin in the phenolic fraction. Adding 25 or 50 µM of H2O2to the cell culture caused oxidative stress, manifested as generation of superoxide and peroxyl radicals, reduced cell viability, G0/G1 arrest, and enhanced apoptosis. This stress was significantly alleviated by the ethanolic and aqueous extracts ofS. hortensisand by the partially purified rosmarinic acid fraction. The application of an aqueousS. hort...
In contemporary literature there are data about significant role of free radical oxidation in the... more In contemporary literature there are data about significant role of free radical oxidation in the pathogenesis of obesity. In this relation application of endogenous as well as exogenous antioxidants for the purpose of excess weight correction has generated increasing interest in scientific circles. Numerous researches showed natural antioxidants to be effective in correction of elevated blood cholesterol, triglycerides, LDL levels. In this relation the purpose of our research was to establish effectiveness of green tea catechines and citrus extract on lipid metabolism disorder and excess body weight during experimental alimentary obesity. Experiment was conducted on rats kept on high-calorie diet for 7 weeks. Simultaneously animals from III and IV group had been administered catechines and citrus extract at the doses of 15 mg and 30 mg respectively in addition to the high-calorie diet during last 4 weeks. The experiment established corrective effect of abovementioned preparations o...
The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular... more The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential ofT. patulacompounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralizati...
Background: Recovery of dental arch defects, related to insertion of a foreign bodies into the or... more Background: Recovery of dental arch defects, related to insertion of a foreign bodies into the oral cavity, and the impact of mechanical pressure, allergic-toxic effects, and the related inflammatory and degenerative processes is one of the most urgent problems in dentistry.The purpose of the study was to evaluate the efficacy of Georgian bean extract tincture as mouthwash for patients using a lever system to correct deformities of the jaw and teeth.Methods: Twenty-eight patients (aged 12-25 years) with deformities of the jaw and teeth were studied. Patients were examined 6-8 months after the bracket system was installed, and before and after the use of tincture mouthwash for 10 days. Patients were randomly divided into 2 groups: the control group for the mouthwash used standard tincture ("Octenidol" Schulke and Mayr -Germany); the patients of the experimental group used tincture made from the Georgian legume crop extracts (GLCE). Before and after the use of both types of ...
Summer savory (Satureja hortensisL.,Lamiaceae) is used in several regions of the world as a spice... more Summer savory (Satureja hortensisL.,Lamiaceae) is used in several regions of the world as a spice and folk medicine. Anti-inflammatory and cytoprotective effects ofS. hortensisand of its rosmarinic acid-rich phenolic fraction have been demonstrated in animal trials. However, previous studies of rosmarinic acid in cell models have yielded controversial results. In this study, we investigated the effects of summer savory extracts on H2O2-challenged human lymphoblastoid Jurkat T cells. LC-MS analysis confirmed the presence of rosmarinic acid and flavonoids such as hesperidin and naringin in the phenolic fraction. Adding 25 or 50 µM of H2O2to the cell culture caused oxidative stress, manifested as generation of superoxide and peroxyl radicals, reduced cell viability, G0/G1 arrest, and enhanced apoptosis. This stress was significantly alleviated by the ethanolic and aqueous extracts ofS. hortensisand by the partially purified rosmarinic acid fraction. The application of an aqueousS. hort...
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Papers by Irakli Chkhikvishvili