International journal of Pharmacy and Pharmaceutical Sciences, Nov 1, 2014
Objective: A substantial fraction of the population is intolerant or does not respond well to the... more Objective: A substantial fraction of the population is intolerant or does not respond well to the recommended treatments for dyslipidemia. The purpose of this study was to evaluate the efficacy of gamma-oryzanol (γ-ORZ) treatment in acute and long-term mouse experimental models of dyslipidemia in comparison to Gemfibrozil and Simvastatin. Methods: For the acute dyslipidemia-induced model, dyslipidemia was induced in 40 mice using a single intra-peritoneal administration of Triton WR-1339. For the long-termmodel, dyslipidemia was induced in 24 mice using a hypercholesterolemic diet over 14 days. Thereafter, animals were divided into different groups of treatment, and orally received treatments with gamma-oryzanol (5, 25, 50mg. kg
International journal of Pharmacy and Pharmaceutical Sciences, Oct 1, 2014
In the present paper, an accurate High Pressure Liquid Chromatography (HPLC) method was developed... more In the present paper, an accurate High Pressure Liquid Chromatography (HPLC) method was developed and validated to determine quantitatively wedelolactone (WL) and demethylwedelolactone Methods: This study presents the High Pressure Liquid Chromatography (HPLC) analysis of the WL and DWL contents in various parts of the plant using different solvents and extraction methods. (DWL) in herbal extract of Eclipta alba (L.) Hassk grown in the Northeast of Brazil. Consequently, an extraction technique was developed using different parts of the plant and the technique was optimized to assess the efficacy of extraction using different solvents. Results: Quantitative HPLC analyses of the methanolic extract obtained via Soxhlet extraction showed that the highest WL concentration was found in the E. alba leaves (1.152 % w/w of dry leaf) and the highest concentrations of DWL were found in the stems (0.395 % w/w of dry stem). Quantitative HPLC analyses also showed a higher concentration of WL (0.271 % w/w of dry whole plant) and DWL (0.184 % w/w of dry whole plant) using water: ethanol extraction, compared with the Soxhlet extraction in methanol, concentration of WL (0.233 % w/w of dry whole plant) and DWL (0.159 % w/w of dry whole plant). Conclusion: HPLC analyses revealed the presence of the two coumestans in all the extracts analysed. Moreover, when analyzing the methanolic E. alba extract, the highest concentrations of
Abstract p-Hydroxycinnamic diester is very common in fruits and beverages. Furthermore, it belong... more Abstract p-Hydroxycinnamic diester is very common in fruits and beverages. Furthermore, it belongs to the family of cinnamic acids. It is a chemical compound present in carnauba leaf powder, and it has promising pharmacological properties against non-transmissible chronic diseases. Then, the objective of this work was characterizing the chemical composition of p-hydroxycinnamic diester obtained from carnauba leaf powder, as thoroughly evaluate its antioxidant profile and to analyze its effect in diabetic mice induced by high-fat diet. Male C57BL/6J mice were induced by 60 days by a high-fat diet and treated for 30 days with p-hydroxycinnamic diester (200 e 400 mg/Kg) and metformin (200 mg/Kg). p-Hydroxycinnamic diester presented chemical structure consistent with the cinnamic acid derivatives reported in scientific literature. Furthermore, p-hydroxycinnamic diester exhibited excellent in vivo and in vitro antioxidant activity, and it also decreased glycemia, MDA, reactive oxygen species in liver tissue, body mass, water consumption of animals. p-Hydroxycinnamic diester had satisfactory results, and this product could be useful for diabetes treatment.
Objective: To evaluate the antidiabetic effect of galactomannans extracted from Adenanthera pavon... more Objective: To evaluate the antidiabetic effect of galactomannans extracted from Adenanthera pavonina's L. seeds (GAP) in streptozotocin (STZ) induced diabetic mice. Methods: The preliminary galactomannan yield from Adenanthera pavonina L. plant and extraction products composition were evaluated. Various chemical characterization methods like thin layer chromatography, Fourier transform infrared spectroscopy, 1H and 13C nuclear magnetic resonance, and molecular weight by gel permeation chromatography have been employed to characterize the extracted galactomannan. The mice were divided in four groups: Normal control, diabetic control, GAP (1% and 2%) treated and standard drug treated groups. Diabetic mice received treatment daily for 30 d. Diabetes was induced by STZ at a single dose of 120 mg/kg. Body weight, water and food intake, fasting blood glucose, total cholesterol and triglycerides were measured. Histopathological analysis of pancreas and liver were performed to evaluate STZ-induced tissue injuries. Results: The isolated and extracted galactomannan from Adenanthera pavonina was confirmed by various chemical characterization methods. GAP exhibited a 1.46:1 mannose: galactose ratio, and high molar weight. Both GAP enriched food decreased glycaemia, total cholesterol and triacylglycerol. GAP didn't interfere on food intakes or body weight, although it increased water intake. Furthermore, the relative liver weight indicated toxic galactomannan effects on the histopathological changes of the pancreas in STZ induced diabetes. Conclusions: It is concluded that GAP is a natural product that contains potent galactomannan and is useful in preventing and treating diabetes.
Journal of Venomous Animals and Toxins Including Tropical Diseases, 2011
In recent years, the search for natural plant products to fight viral diseases has been increasin... more In recent years, the search for natural plant products to fight viral diseases has been increasing. In this work, two Spondias species, namely S. mombin and S. tuberosa, found in Ceara state (Brazil), and their main phenolic components were evaluated against dengue virus. In vitro antiviral tests were performed against type-2 dengue virus by the MTT method and standard cytopathic effect reduction assay in C6/36 cells. Cytotoxicity was also evaluated by MTT. The presence of phenolic compounds quercetin, rutin, and ellagic acid in plant extracts was characterized by HPLC analysis. Both Spondias species extracts and components were nontoxic to the cells whereas rutin and quercetin displayed relevant antiviral activity with IC50 of 362.68 µg/mL and 500 µg/mL, respectively.
International journal of Pharmacy and Pharmaceutical Sciences, Nov 1, 2014
Objective: A substantial fraction of the population is intolerant or does not respond well to the... more Objective: A substantial fraction of the population is intolerant or does not respond well to the recommended treatments for dyslipidemia. The purpose of this study was to evaluate the efficacy of gamma-oryzanol (γ-ORZ) treatment in acute and long-term mouse experimental models of dyslipidemia in comparison to Gemfibrozil and Simvastatin. Methods: For the acute dyslipidemia-induced model, dyslipidemia was induced in 40 mice using a single intra-peritoneal administration of Triton WR-1339. For the long-termmodel, dyslipidemia was induced in 24 mice using a hypercholesterolemic diet over 14 days. Thereafter, animals were divided into different groups of treatment, and orally received treatments with gamma-oryzanol (5, 25, 50mg. kg
International journal of Pharmacy and Pharmaceutical Sciences, Oct 1, 2014
In the present paper, an accurate High Pressure Liquid Chromatography (HPLC) method was developed... more In the present paper, an accurate High Pressure Liquid Chromatography (HPLC) method was developed and validated to determine quantitatively wedelolactone (WL) and demethylwedelolactone Methods: This study presents the High Pressure Liquid Chromatography (HPLC) analysis of the WL and DWL contents in various parts of the plant using different solvents and extraction methods. (DWL) in herbal extract of Eclipta alba (L.) Hassk grown in the Northeast of Brazil. Consequently, an extraction technique was developed using different parts of the plant and the technique was optimized to assess the efficacy of extraction using different solvents. Results: Quantitative HPLC analyses of the methanolic extract obtained via Soxhlet extraction showed that the highest WL concentration was found in the E. alba leaves (1.152 % w/w of dry leaf) and the highest concentrations of DWL were found in the stems (0.395 % w/w of dry stem). Quantitative HPLC analyses also showed a higher concentration of WL (0.271 % w/w of dry whole plant) and DWL (0.184 % w/w of dry whole plant) using water: ethanol extraction, compared with the Soxhlet extraction in methanol, concentration of WL (0.233 % w/w of dry whole plant) and DWL (0.159 % w/w of dry whole plant). Conclusion: HPLC analyses revealed the presence of the two coumestans in all the extracts analysed. Moreover, when analyzing the methanolic E. alba extract, the highest concentrations of
Abstract p-Hydroxycinnamic diester is very common in fruits and beverages. Furthermore, it belong... more Abstract p-Hydroxycinnamic diester is very common in fruits and beverages. Furthermore, it belongs to the family of cinnamic acids. It is a chemical compound present in carnauba leaf powder, and it has promising pharmacological properties against non-transmissible chronic diseases. Then, the objective of this work was characterizing the chemical composition of p-hydroxycinnamic diester obtained from carnauba leaf powder, as thoroughly evaluate its antioxidant profile and to analyze its effect in diabetic mice induced by high-fat diet. Male C57BL/6J mice were induced by 60 days by a high-fat diet and treated for 30 days with p-hydroxycinnamic diester (200 e 400 mg/Kg) and metformin (200 mg/Kg). p-Hydroxycinnamic diester presented chemical structure consistent with the cinnamic acid derivatives reported in scientific literature. Furthermore, p-hydroxycinnamic diester exhibited excellent in vivo and in vitro antioxidant activity, and it also decreased glycemia, MDA, reactive oxygen species in liver tissue, body mass, water consumption of animals. p-Hydroxycinnamic diester had satisfactory results, and this product could be useful for diabetes treatment.
Objective: To evaluate the antidiabetic effect of galactomannans extracted from Adenanthera pavon... more Objective: To evaluate the antidiabetic effect of galactomannans extracted from Adenanthera pavonina's L. seeds (GAP) in streptozotocin (STZ) induced diabetic mice. Methods: The preliminary galactomannan yield from Adenanthera pavonina L. plant and extraction products composition were evaluated. Various chemical characterization methods like thin layer chromatography, Fourier transform infrared spectroscopy, 1H and 13C nuclear magnetic resonance, and molecular weight by gel permeation chromatography have been employed to characterize the extracted galactomannan. The mice were divided in four groups: Normal control, diabetic control, GAP (1% and 2%) treated and standard drug treated groups. Diabetic mice received treatment daily for 30 d. Diabetes was induced by STZ at a single dose of 120 mg/kg. Body weight, water and food intake, fasting blood glucose, total cholesterol and triglycerides were measured. Histopathological analysis of pancreas and liver were performed to evaluate STZ-induced tissue injuries. Results: The isolated and extracted galactomannan from Adenanthera pavonina was confirmed by various chemical characterization methods. GAP exhibited a 1.46:1 mannose: galactose ratio, and high molar weight. Both GAP enriched food decreased glycaemia, total cholesterol and triacylglycerol. GAP didn't interfere on food intakes or body weight, although it increased water intake. Furthermore, the relative liver weight indicated toxic galactomannan effects on the histopathological changes of the pancreas in STZ induced diabetes. Conclusions: It is concluded that GAP is a natural product that contains potent galactomannan and is useful in preventing and treating diabetes.
Journal of Venomous Animals and Toxins Including Tropical Diseases, 2011
In recent years, the search for natural plant products to fight viral diseases has been increasin... more In recent years, the search for natural plant products to fight viral diseases has been increasing. In this work, two Spondias species, namely S. mombin and S. tuberosa, found in Ceara state (Brazil), and their main phenolic components were evaluated against dengue virus. In vitro antiviral tests were performed against type-2 dengue virus by the MTT method and standard cytopathic effect reduction assay in C6/36 cells. Cytotoxicity was also evaluated by MTT. The presence of phenolic compounds quercetin, rutin, and ellagic acid in plant extracts was characterized by HPLC analysis. Both Spondias species extracts and components were nontoxic to the cells whereas rutin and quercetin displayed relevant antiviral activity with IC50 of 362.68 µg/mL and 500 µg/mL, respectively.
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