Heat shock induces the transcriptional activation of large heterochromatic regions of the human genome composed of arrays of satellite III DNA repeats. A number of RNA-processing factors, among them splicing factor SF2/ASF, associate with... more
Heat shock induces the transcriptional activation of large heterochromatic regions of the human genome composed of arrays of satellite III DNA repeats. A number of RNA-processing factors, among them splicing factor SF2/ASF, associate with these transcription factors giving rise to nuclear stress bodies (nSBs). Here, we show that the recruitment of SF2/ASF to these structures is mediated by its second RNA recognition motif. Amino acid substitutions in the first alpha-helix of this domain, but not in the beta-strand regions, abrogate the association with nSBs. The same mutations drastically affect the in vivo activity of SF2/ASF in the alternative splicing of adenoviral E1A transcripts. Sequence analysis identifies four putative high-affinity binding sites for SF2/ASF in the transcribed strand of the satellite III DNA. We have verified by gel mobility shift assays that the second RNA-binding domain of SF2/ASF binds at least one of these sites. Our analysis suggests that the recruitmen...
Research Interests: M Rna Processing, Sequence Analysis, Biological Sciences, Environmental Sciences, Humans, and 13 moreAlternative splicing, Nucleic Acids, Transcription Factor, Human Genome, RNA-binding proteins, HeLa cells, Heat Shock, Amino Acid Profile, Alternative Splicing, Amino Acid Sequence, Amino Acid Substitution Rates, Binding Site, and Protein Transport
Thermal and chemical ,stresses induce the formation in human ,cells of novel ,and transient nuclear structures called nuclearstress bodies (nSBs). These contain heat shock factor 1 (HSF1) and a specific subset of pre-mRNA processing... more
Thermal and chemical ,stresses induce the formation in human ,cells of novel ,and transient nuclear structures called nuclearstress bodies (nSBs). These contain heat shock factor 1 (HSF1) and a specific subset of pre-mRNA processing factors. Nuclear stress bodies are assembled,on specific pericentromeric ,heterochromatic ,domains containing satellite III (SatIII) DNA. In response,to stress ,these domains ,change ,their epigenetic status from
Heat shock triggers the assembly of nuclear stress bodies that contain heat shock factor 1 and a subset of RNA processing factors. These structures are formed on the pericentromeric heterochromatic regions of specific human chromosomes,... more
Heat shock triggers the assembly of nuclear stress bodies that contain heat shock factor 1 and a subset of RNA processing factors. These structures are formed on the pericentromeric heterochromatic regions of specific human chromosomes, among which chromosome 9. In this article we show that these heterochromatic domains are characterized by an epigenetic status typical of euchromatic regions. Similarly to