Squamous metaplasia in transitional cell carcinoma of urinary bladder is considered as an adverse... more Squamous metaplasia in transitional cell carcinoma of urinary bladder is considered as an adverse prognostic factor. Those patients have weaker response on therapy and lower survival rate. The aim of our study was to determine the incidence of squamous metaplasia in bioptic material according to histologic grade and growth pattern. In our study we used pathohistological data of 1781 patients operated for urinary bladder cancer in Department of Urology of Sestre milosrdnice University Hospital during the period from 1989 to 2000 squamousmetaplasia was found in 5.7% of patients. According to growth pattern, this phenomenon is more frequent in patients with solid growth pattern. In papillary cancers, the higher incidence of squamousmetaplasia was determined in G1 and G2 histologic grade, while in solid growth pattern, the most cases with squamous metaplasia or over 90% were of G3 histologic grade. Muscle layer invasion were present in high percentage of both, papillary and solid urothe...
Due to the rarity of multiple primary synchronous malignancies, the correct and concurring data o... more Due to the rarity of multiple primary synchronous malignancies, the correct and concurring data on their incidence are extremely difficult to find. Recent studies point to renal cell carcinoma as a tumor showing most prominent association with other types of simultaneous or metachronous primary carcinoma. To our knowledge, this is the first case of multiple primary adenocarcinomas of the kidney, prostate, and urothelial carcinoma of the urinary bladder associated with primary sigmoid adenocarcinoma and colostomic metastasis analyzed for p53 loss of heterozygosity.
The report covers the first case of insular thyroid cancer recorded in Croatian medical literatur... more The report covers the first case of insular thyroid cancer recorded in Croatian medical literature. A 71-year old female patient presented to our hospital with symptoms of inspiratory stridor. Clinical examination disclosed an expansive tumor mass. US guided FNA indicated anaplastic cancer and total thyroidectomy was accordingly indicated. Pathohistological analysis of H/E biopsies disclosed a tumor mass with a characteristic insular growth pattern of monomorphic tumor cells. Immunohistochemical analysis confirmed diagnosis by a diffuse positive reaction to thyreoglobulin, focally to TTF-1, and scant to cytokeratin, while calcitonin, FVIII, CD34 and LCA were clearly negative. P53 and bcl-2 were expressed in 35.9% and 85% of tumor cells, respectively. The proliferation index for Ki-67 was 4.19. According to our survey we suggest a panel of immunohistochemistry for diagnosing insular cancer. Even if partly present, insular cancer should be mentioned in the pathohistological descriptio...
Acta medica Croatica : c̆asopis Hravatske akademije medicinskih znanosti, 2005
Among multiple primary cancers in the same patient, renal cell carcinoma occurring either synchro... more Among multiple primary cancers in the same patient, renal cell carcinoma occurring either synchronously or metachronously is one of the most common. The aim of the study was to determine the frequency of second primary malignant tumor in patients with primary renal cell carcinoma. Between 1992 and 2000, 447 patients underwent nephrectomy for primary renal tumor at the Department of Urology, Sestre milosrdnice University Hospital. Out of 447 patients 310 were registered at Cancer Registry of the Republic of Croatia. There were data on 297 patients with renal cell carcinoma, 197 male and 104 female patients, age group 24-91 (mean 59.9) years. Data on 24 (8.1%) patients with second primary malignant tumors were found in Hospital Registry and Croatian Cancer Registry during the study period. There were 13 male patients, age range 47-80 (median 65.1) years, and 11 female patients, age range 51-70 years (median 61.1) years with 26 second primary tumors. One male patient presented with fou...
Recent publications of breast cancer classification based on gene expression profile analyses ind... more Recent publications of breast cancer classification based on gene expression profile analyses indicate that medullary breast carcinomas (MBC) may be considered part of the basal-like carcinoma spectrum made up of ER-negative, PR-negative and HER-2-negative cells ("triple-negative phenotype"). On the other hand, there are also data showing that a proportion of MBC and atypical MBC (AMBC) is ER, PR and/or HER-2 positive. Therefore, we have decided to immunohistochemically analyze ER, PR, HER-2 and basal/myoepithelial markers CK 5/6, p63 and EGFR expression in our archival paraffin-embedded MBC and AMBC samples from 48 patients. Immunohistochemical evaluation of samples which were derived from patients operated on at our two hospitals between 1999 and 2005. Typical MBC was found in 39 patients and AMBC in 9 patients. The patients ranged in age from 32 to 84 years (median 55). Modified radical mastectomy with axillary dissection was performed in 30/48 patients (63%) while brea...
antigens in medullary breast cancer: retrospective immunohistochemical study Aim To immunohistoch... more antigens in medullary breast cancer: retrospective immunohistochemical study Aim To immunohistochemically evaluate the expression of MAGE-A1, MAGE-A, and NY-ESO-1 cancer/testis (C/T) tumor antigens in medullary breast cancer (MBC) tumor samples and to analyze it in relation to the clinicopathological features. Methods This retrospective study included samples from 49 patients: 40 with typical MBC and 9 with atypical MBC. Tumor specimens were obtained from patients operated on in the University Hospital for Tumors and the Sisters of Mercy University Hospital, Zagreb, Croatia, from 1999 to 2005. Standard immunohistochemistry was used on archival paraffin-embedded MBC tissues. Results MAGE-A1, MAGE-A, and NY-ESO-1 antigens were expressed in 33 % (16/49), 33 % (16/49), and 22 % (11/49) of patients, respectively. No difference between the groups with and without C/T tumor antigen expression in age at diagnosis, tumor size, axillary lymph node metastasis, adjuvant therapy, and HER-2 expre...
SUMMARYAlthough melanoma is less common than other types of malignant skin tumors, in- cluding ba... more SUMMARYAlthough melanoma is less common than other types of malignant skin tumors, in- cluding basal and squamous cell cancers, it accounts for about 75% of all skin cancer deaths. Mela- noma is much more likely to metastasize to other parts of the body, which is not the case with other types of skin tumors. Metastatic spread is very common, particularly in advanced tumors of higher Clark and Breslow stage. The aim of the study was to analyze the prevalence and distribution of metastatic melanomas among biopsy specimens collected during the 1995-2000 period, and their relationship with primary melanoma, especially regarding Clark and Breslow staging. Metastatic mela- nomas were found in 210 of 75,390 (0.3%) surgical biopsies. There were 24 patients with 57 meta- static melanoma biopsies related to primary melanoma diagnosed at our hospital during the period of observation. The remaining 153 metastatic melanomas diagnosed at other hospitals or before the period of observation were no...
Department of Gastroenterology, Sestre milosrdnice University Hospital, Zagreb, CroatiaSUMMARY Œ ... more Department of Gastroenterology, Sestre milosrdnice University Hospital, Zagreb, CroatiaSUMMARY Œ Hemangiopericytoma is a rare malignant vascular tumor deriving from pericytes. It usuallyoccurs in the extremities and retroperitoneum, and extremely rarely in the gastrointestinal tract. A case ofhemangiopericytoma in the gastroesophageal region is reported. A 56-year-old man was treated with H2-receptor antagonist for dyspeptic symptoms that had occurred three months before. Therapy failed toprove successful and gastroendoscopy was performed in March 2001 to reveal slightly reddish ventricularmucosa with normal rugal folds and a sessile, polypoid, grayish mass of rough surface located at the gastroe-sophageal junction. Histopathologic examination of polypoid mass samples revealed a vascular tumor cov-ered with squamous epithelium. The hallmarks of the tumor were numerous branching, slit-like capillarychannels with ‚staghorn™ appearance, and large gaping sinusoidal spaces enclosed withi...
The aim of the study was to determine the frequency of second primary malignant tumor in patients... more The aim of the study was to determine the frequency of second primary malignant tumor in patients with primary renal cell carcinoma. Between 1992 and 2000, 447 patients underwent nephrectomy for primary renal tumor at the Department of Urology, Sestre milosrdnice University Hospital. Second primary malignant tumors were found in 24 (8.1%) patients during the study period. There were 13 male patients, age range 47-80 (median 65.1) years, and 11 female patients, age range 51-70 years (median 61.1) years with 26 second primary tumors. One male patient presented with four different primary tumors (kidney, prostate, urinary bladder and colon). The patients most commonly presented with prostate and colon carcinoma. Results indicate that during the clinical follow-up of patients treated for primary renal cell cancer, a possibility of second primary cancers should always be considered.
Tumori zametnih stanica predstavljaju 95% svih tumora testisa i cesto su sastavljeni od vise razl... more Tumori zametnih stanica predstavljaju 95% svih tumora testisa i cesto su sastavljeni od vise razlicitih komponenata. Najbolju prognozu imaju seminomi, dok prognoza neseminomskih tumora ovisi o zastupljenosti i vrsti razlicitih komponenata. Cilj ovog rada je odrediti zastupljenost i histoloski tip razlicitih sastavnica tumora zametnih stanica testisa dijagnosticiranih u razdoblju 1992-2000. U istraživanom razdoblju bilo je ukupno 131 takvih tumora od cega je bilo 72 (55, 0%) neseminomska i 59 (45, 0%) seminomskih tumora. Od 72 neseminomska tumora 4 su bila sastavljena samo od jedne komponente dok je 68 bilo mijesanih. Neseminomski tumori su najcesce sadržavali komponente embrionalnog karcinoma (91, 7%), teratoma (70, 8%) i yolk sac tumora (33, 3%). Najcesca kombinacija neseminomskog tumora sastojala se od embrionalnog karcinoma i teratoma u 28 (41, 2%) slucajeva. Seminom je dijagnosticiran u 59 tumora, ali je komponenta seminoma nađena u jos 19 (26, 4%) slucajeva mijesanih tumora zam...
Squamous metaplasia in transitional cell carcinoma of urinary bladder is considered as an adverse... more Squamous metaplasia in transitional cell carcinoma of urinary bladder is considered as an adverse prognostic factor. Those patients have weaker response on therapy and lower survival rate. The aim of our study was to determine the incidence of squamous metaplasia in bioptic material according to histologic grade and growth pattern. In our study we used pathohistological data of 1781 patients operated for urinary bladder cancer in Department of Urology of Sestre milosrdnice University Hospital during the period from 1989 to 2000 squamousmetaplasia was found in 5.7% of patients. According to growth pattern, this phenomenon is more frequent in patients with solid growth pattern. In papillary cancers, the higher incidence of squamousmetaplasia was determined in G1 and G2 histologic grade, while in solid growth pattern, the most cases with squamous metaplasia or over 90% were of G3 histologic grade. Muscle layer invasion were present in high percentage of both, papillary and solid urothe...
Due to the rarity of multiple primary synchronous malignancies, the correct and concurring data o... more Due to the rarity of multiple primary synchronous malignancies, the correct and concurring data on their incidence are extremely difficult to find. Recent studies point to renal cell carcinoma as a tumor showing most prominent association with other types of simultaneous or metachronous primary carcinoma. To our knowledge, this is the first case of multiple primary adenocarcinomas of the kidney, prostate, and urothelial carcinoma of the urinary bladder associated with primary sigmoid adenocarcinoma and colostomic metastasis analyzed for p53 loss of heterozygosity.
The report covers the first case of insular thyroid cancer recorded in Croatian medical literatur... more The report covers the first case of insular thyroid cancer recorded in Croatian medical literature. A 71-year old female patient presented to our hospital with symptoms of inspiratory stridor. Clinical examination disclosed an expansive tumor mass. US guided FNA indicated anaplastic cancer and total thyroidectomy was accordingly indicated. Pathohistological analysis of H/E biopsies disclosed a tumor mass with a characteristic insular growth pattern of monomorphic tumor cells. Immunohistochemical analysis confirmed diagnosis by a diffuse positive reaction to thyreoglobulin, focally to TTF-1, and scant to cytokeratin, while calcitonin, FVIII, CD34 and LCA were clearly negative. P53 and bcl-2 were expressed in 35.9% and 85% of tumor cells, respectively. The proliferation index for Ki-67 was 4.19. According to our survey we suggest a panel of immunohistochemistry for diagnosing insular cancer. Even if partly present, insular cancer should be mentioned in the pathohistological descriptio...
Acta medica Croatica : c̆asopis Hravatske akademije medicinskih znanosti, 2005
Among multiple primary cancers in the same patient, renal cell carcinoma occurring either synchro... more Among multiple primary cancers in the same patient, renal cell carcinoma occurring either synchronously or metachronously is one of the most common. The aim of the study was to determine the frequency of second primary malignant tumor in patients with primary renal cell carcinoma. Between 1992 and 2000, 447 patients underwent nephrectomy for primary renal tumor at the Department of Urology, Sestre milosrdnice University Hospital. Out of 447 patients 310 were registered at Cancer Registry of the Republic of Croatia. There were data on 297 patients with renal cell carcinoma, 197 male and 104 female patients, age group 24-91 (mean 59.9) years. Data on 24 (8.1%) patients with second primary malignant tumors were found in Hospital Registry and Croatian Cancer Registry during the study period. There were 13 male patients, age range 47-80 (median 65.1) years, and 11 female patients, age range 51-70 years (median 61.1) years with 26 second primary tumors. One male patient presented with fou...
Recent publications of breast cancer classification based on gene expression profile analyses ind... more Recent publications of breast cancer classification based on gene expression profile analyses indicate that medullary breast carcinomas (MBC) may be considered part of the basal-like carcinoma spectrum made up of ER-negative, PR-negative and HER-2-negative cells ("triple-negative phenotype"). On the other hand, there are also data showing that a proportion of MBC and atypical MBC (AMBC) is ER, PR and/or HER-2 positive. Therefore, we have decided to immunohistochemically analyze ER, PR, HER-2 and basal/myoepithelial markers CK 5/6, p63 and EGFR expression in our archival paraffin-embedded MBC and AMBC samples from 48 patients. Immunohistochemical evaluation of samples which were derived from patients operated on at our two hospitals between 1999 and 2005. Typical MBC was found in 39 patients and AMBC in 9 patients. The patients ranged in age from 32 to 84 years (median 55). Modified radical mastectomy with axillary dissection was performed in 30/48 patients (63%) while brea...
antigens in medullary breast cancer: retrospective immunohistochemical study Aim To immunohistoch... more antigens in medullary breast cancer: retrospective immunohistochemical study Aim To immunohistochemically evaluate the expression of MAGE-A1, MAGE-A, and NY-ESO-1 cancer/testis (C/T) tumor antigens in medullary breast cancer (MBC) tumor samples and to analyze it in relation to the clinicopathological features. Methods This retrospective study included samples from 49 patients: 40 with typical MBC and 9 with atypical MBC. Tumor specimens were obtained from patients operated on in the University Hospital for Tumors and the Sisters of Mercy University Hospital, Zagreb, Croatia, from 1999 to 2005. Standard immunohistochemistry was used on archival paraffin-embedded MBC tissues. Results MAGE-A1, MAGE-A, and NY-ESO-1 antigens were expressed in 33 % (16/49), 33 % (16/49), and 22 % (11/49) of patients, respectively. No difference between the groups with and without C/T tumor antigen expression in age at diagnosis, tumor size, axillary lymph node metastasis, adjuvant therapy, and HER-2 expre...
SUMMARYAlthough melanoma is less common than other types of malignant skin tumors, in- cluding ba... more SUMMARYAlthough melanoma is less common than other types of malignant skin tumors, in- cluding basal and squamous cell cancers, it accounts for about 75% of all skin cancer deaths. Mela- noma is much more likely to metastasize to other parts of the body, which is not the case with other types of skin tumors. Metastatic spread is very common, particularly in advanced tumors of higher Clark and Breslow stage. The aim of the study was to analyze the prevalence and distribution of metastatic melanomas among biopsy specimens collected during the 1995-2000 period, and their relationship with primary melanoma, especially regarding Clark and Breslow staging. Metastatic mela- nomas were found in 210 of 75,390 (0.3%) surgical biopsies. There were 24 patients with 57 meta- static melanoma biopsies related to primary melanoma diagnosed at our hospital during the period of observation. The remaining 153 metastatic melanomas diagnosed at other hospitals or before the period of observation were no...
Department of Gastroenterology, Sestre milosrdnice University Hospital, Zagreb, CroatiaSUMMARY Œ ... more Department of Gastroenterology, Sestre milosrdnice University Hospital, Zagreb, CroatiaSUMMARY Œ Hemangiopericytoma is a rare malignant vascular tumor deriving from pericytes. It usuallyoccurs in the extremities and retroperitoneum, and extremely rarely in the gastrointestinal tract. A case ofhemangiopericytoma in the gastroesophageal region is reported. A 56-year-old man was treated with H2-receptor antagonist for dyspeptic symptoms that had occurred three months before. Therapy failed toprove successful and gastroendoscopy was performed in March 2001 to reveal slightly reddish ventricularmucosa with normal rugal folds and a sessile, polypoid, grayish mass of rough surface located at the gastroe-sophageal junction. Histopathologic examination of polypoid mass samples revealed a vascular tumor cov-ered with squamous epithelium. The hallmarks of the tumor were numerous branching, slit-like capillarychannels with ‚staghorn™ appearance, and large gaping sinusoidal spaces enclosed withi...
The aim of the study was to determine the frequency of second primary malignant tumor in patients... more The aim of the study was to determine the frequency of second primary malignant tumor in patients with primary renal cell carcinoma. Between 1992 and 2000, 447 patients underwent nephrectomy for primary renal tumor at the Department of Urology, Sestre milosrdnice University Hospital. Second primary malignant tumors were found in 24 (8.1%) patients during the study period. There were 13 male patients, age range 47-80 (median 65.1) years, and 11 female patients, age range 51-70 years (median 61.1) years with 26 second primary tumors. One male patient presented with four different primary tumors (kidney, prostate, urinary bladder and colon). The patients most commonly presented with prostate and colon carcinoma. Results indicate that during the clinical follow-up of patients treated for primary renal cell cancer, a possibility of second primary cancers should always be considered.
Tumori zametnih stanica predstavljaju 95% svih tumora testisa i cesto su sastavljeni od vise razl... more Tumori zametnih stanica predstavljaju 95% svih tumora testisa i cesto su sastavljeni od vise razlicitih komponenata. Najbolju prognozu imaju seminomi, dok prognoza neseminomskih tumora ovisi o zastupljenosti i vrsti razlicitih komponenata. Cilj ovog rada je odrediti zastupljenost i histoloski tip razlicitih sastavnica tumora zametnih stanica testisa dijagnosticiranih u razdoblju 1992-2000. U istraživanom razdoblju bilo je ukupno 131 takvih tumora od cega je bilo 72 (55, 0%) neseminomska i 59 (45, 0%) seminomskih tumora. Od 72 neseminomska tumora 4 su bila sastavljena samo od jedne komponente dok je 68 bilo mijesanih. Neseminomski tumori su najcesce sadržavali komponente embrionalnog karcinoma (91, 7%), teratoma (70, 8%) i yolk sac tumora (33, 3%). Najcesca kombinacija neseminomskog tumora sastojala se od embrionalnog karcinoma i teratoma u 28 (41, 2%) slucajeva. Seminom je dijagnosticiran u 59 tumora, ali je komponenta seminoma nađena u jos 19 (26, 4%) slucajeva mijesanih tumora zam...
Uploads
Papers by Irena Novosel