Medical science monitor : international medical journal of experimental and clinical research, 2010
Hypertension is a common disease of the cardiovascular system and one of the main causes of morta... more Hypertension is a common disease of the cardiovascular system and one of the main causes of mortality in the world. Its etiopathogenesis and molecular mechanisms are unknown. Epigenetic changes may play a role in its development. Therefore the level of 5-methylcytosine (5mC), a well-known epigenetic marker, was analyzed in DNA from the blood of essential hypertension patients. TLC chromatographic analysis of the DNA nucleotide composition was used to determine 5mC levels in blood DNA samples from 60 patients suffering from essential hypertension (30 with stage 1 and 30 with stage 2 hypertension) and 30 control subjects. The mean levels of 5mC were 1.80 + or - 0.69 in the healthy subjects, 1.14 + or - 0.48 in all the patients with essential hypertension, 1.29 + or - 0.50 in those with stage 1, and 0.99 + or - 0.42 in those with stage 2 of hypertension. Statistically significant differences in 5mC amount in DNA were observed between the control group and the whole patient group, the c...
Glioblastoma multiforme (GBM) is the most common form of malignant glioma, characterized by genet... more Glioblastoma multiforme (GBM) is the most common form of malignant glioma, characterized by genetic instability, intratumoral histopathological variability, and unpredictable clinical behavior. Malignant gliomas express preferentially a number of surface markers that may be exploited as therapeutic targets, such as tenascin-C, an extracellular matrix glycoprotein contributes to tumor cell adhesion, invasion, migration and proliferation. Disappointing results in the treatment of gliomas with surgery, radiation and chemotherapy have fuelled a search for new treatment modalities. Here we present the data for 46 patients suffering from brain tumor. They were resected and treated with dsRNA (ATN-RNA) complementary to the sequence of tenascin-C mRNA. MRI and CT follow up studies showed growth tumor delay or lack of its recurrence symptoms, due to inhibition of TN-C synthesis. A significant improvement in overall survival (OS) was observed without loosing of the quality of life (QOL) of patients. This novel therapy based on RNA interference shows a big therapeutical potential. To our knowledge intervention with RNAi (iRNAi) is the first protocol of application of RNAi in human disease treatment.
Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GB... more Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GBM remains refractory to therapy because of tumor heterogeneity, local invasion, and non-uniform vascular permeability to drugs. Patients with GBM have the median survival of approximately 8-10 months, and for those cases where tumor recurs, the average time of tumor progression after therapy is only eight weeks. A combination of different treatment modes as surgery and chemo- or/and radiotherapy extend survival only for a short time, if any. Recently, tenascin-C (TN-C) as a dominant epitope in glioblastoma has been discovered. It is transiently expressed during organogenesis, absent or much reduced in most fully developed organs, but reappears under pathological conditions such as infection, inflammation, or tumorigenesis. It was found that the intensity of TN-C staining correlates with the tumor grade and that the strongest staining indicates poor prognosis.
Therapeutic Ribonucleic Acids in Brain Tumors, 2009
We describe a new, simple and reliable method for diagnosis of a neoplastic disease. It is based ... more We describe a new, simple and reliable method for diagnosis of a neoplastic disease. It is based on the cellulose thin layer chromatography (TLC) quantitative determination of 5-methylcytosine (m5C) in DNA from tumor tissue. Currently, there is a wealth of data showing that oxidative stress effecting through reactive oxygen species (ROS) plays a critical role in the etiology and progression of a number of human diseases. Oxidative damage to DNA, lipids, and proteins is deleterious for the cell. 5-methylcytosine, along with other DNA constituents, is a target for ROS, which results in the appearance of a variety of modified nucleic acid bases. Therefore, m5C moiety constitutes the mutational hotspot; it occurs either within a structural gene or its regulatory regions. Here, we show the results of the analysis of DNA extracted from brain tumor and other tissues. DNA was isolated and sheared by enzymatic digestion into nucleotides which, after labeling with [γ-32p]ATP, were separated on cellulose TLC. A chromatogram was evaluated using phosphoimager and an amount of m5dC was calculated as a ratio (R) of m5dC to m5dC+dC+dT spots intensities. The R values decrease as the malignancy of brain tumors increase. It is the important factor for differentiating low and high grade gliomas. It seems that DNA methylation pattern might be a useful tool for the diagnosis of brain tumors or as a marker for the early detection of the relapse of the disease.
Medical science monitor : international medical journal of experimental and clinical research, 2010
Hypertension is a common disease of the cardiovascular system and one of the main causes of morta... more Hypertension is a common disease of the cardiovascular system and one of the main causes of mortality in the world. Its etiopathogenesis and molecular mechanisms are unknown. Epigenetic changes may play a role in its development. Therefore the level of 5-methylcytosine (5mC), a well-known epigenetic marker, was analyzed in DNA from the blood of essential hypertension patients. TLC chromatographic analysis of the DNA nucleotide composition was used to determine 5mC levels in blood DNA samples from 60 patients suffering from essential hypertension (30 with stage 1 and 30 with stage 2 hypertension) and 30 control subjects. The mean levels of 5mC were 1.80 + or - 0.69 in the healthy subjects, 1.14 + or - 0.48 in all the patients with essential hypertension, 1.29 + or - 0.50 in those with stage 1, and 0.99 + or - 0.42 in those with stage 2 of hypertension. Statistically significant differences in 5mC amount in DNA were observed between the control group and the whole patient group, the c...
The new, simple, and reliable method for the diagnosis of brain tumors is described. It is based ... more The new, simple, and reliable method for the diagnosis of brain tumors is described. It is based on a TLC quantitative determination of 5-methylcytosine (m(5)C) in relation to its damage products of DNA from tumor tissue. Currently, there is evidence that oxidative stress through reactive oxygen species (ROS) plays an important role in the etiology and progression of several human diseases. Oxidative damage of DNA, lipids, and proteins is deleterious for the cell. m(5)C, along with other basic components of DNA, is the target for ROS, which results in the appearance of new modified nucleic acid bases. If so, m(5)C residue constitutes a mutational hotspot position, whether it occurs within a nucleotide sequence of a structural gene or a regulatory region. Here, we show the results of the analysis of 82 DNA samples taken from brain tumor tissues. DNA was isolated and hydrolyzed into nucleotides, which, after labeling with [gamma-(32)P]ATP, were separated on TLC. Chromatograms were eva...
Therapeutic Ribonucleic Acids in Brain Tumors, 2009
Malignant gliomas preferentially express a number of surface markers that may be exploited as the... more Malignant gliomas preferentially express a number of surface markers that may be exploited as therapeutic targets, such as tenascin-C, an extracellular matrix glycoprotein which contributes to tumor cell adhesion, invasion, migration, and proliferation. Glioblastoma ...
Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GB... more Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GBM remains refractory to therapy because of tumor heterogeneity, local invasion, and non-uniform vascular permeability to drugs. Patients with GBM have the median survival of approximately 8-10 months, and for those cases where tumor recurs, the average time of tumor progression after therapy is only eight weeks. A combination of different treatment modes as surgery and chemo- or/and radiotherapy extend survival only for a short time, if any. Recently, tenascin-C (TN-C) as a dominant epitope in glioblastoma has been discovered. It is transiently expressed during organogenesis, absent or much reduced in most fully developed organs, but reappears under pathological conditions such as infection, inflammation, or tumorigenesis. It was found that the intensity of TN-C staining correlates with the tumor grade and that the strongest staining indicates poor prognosis.
We analyze the structure of napin (BngNAP1), a storage protein (mw 14.5 kDa) from Brassica napus.... more We analyze the structure of napin (BngNAP1), a storage protein (mw 14.5 kDa) from Brassica napus. On the basis of the results of 1 H NMR spectroscopy and dynamic light scattering (DLS) studies, the overall shape and secondary structure of the molecule are ...
Medical science monitor : international medical journal of experimental and clinical research, 2010
Hypertension is a common disease of the cardiovascular system and one of the main causes of morta... more Hypertension is a common disease of the cardiovascular system and one of the main causes of mortality in the world. Its etiopathogenesis and molecular mechanisms are unknown. Epigenetic changes may play a role in its development. Therefore the level of 5-methylcytosine (5mC), a well-known epigenetic marker, was analyzed in DNA from the blood of essential hypertension patients. TLC chromatographic analysis of the DNA nucleotide composition was used to determine 5mC levels in blood DNA samples from 60 patients suffering from essential hypertension (30 with stage 1 and 30 with stage 2 hypertension) and 30 control subjects. The mean levels of 5mC were 1.80 + or - 0.69 in the healthy subjects, 1.14 + or - 0.48 in all the patients with essential hypertension, 1.29 + or - 0.50 in those with stage 1, and 0.99 + or - 0.42 in those with stage 2 of hypertension. Statistically significant differences in 5mC amount in DNA were observed between the control group and the whole patient group, the c...
Glioblastoma multiforme (GBM) is the most common form of malignant glioma, characterized by genet... more Glioblastoma multiforme (GBM) is the most common form of malignant glioma, characterized by genetic instability, intratumoral histopathological variability, and unpredictable clinical behavior. Malignant gliomas express preferentially a number of surface markers that may be exploited as therapeutic targets, such as tenascin-C, an extracellular matrix glycoprotein contributes to tumor cell adhesion, invasion, migration and proliferation. Disappointing results in the treatment of gliomas with surgery, radiation and chemotherapy have fuelled a search for new treatment modalities. Here we present the data for 46 patients suffering from brain tumor. They were resected and treated with dsRNA (ATN-RNA) complementary to the sequence of tenascin-C mRNA. MRI and CT follow up studies showed growth tumor delay or lack of its recurrence symptoms, due to inhibition of TN-C synthesis. A significant improvement in overall survival (OS) was observed without loosing of the quality of life (QOL) of patients. This novel therapy based on RNA interference shows a big therapeutical potential. To our knowledge intervention with RNAi (iRNAi) is the first protocol of application of RNAi in human disease treatment.
Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GB... more Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GBM remains refractory to therapy because of tumor heterogeneity, local invasion, and non-uniform vascular permeability to drugs. Patients with GBM have the median survival of approximately 8-10 months, and for those cases where tumor recurs, the average time of tumor progression after therapy is only eight weeks. A combination of different treatment modes as surgery and chemo- or/and radiotherapy extend survival only for a short time, if any. Recently, tenascin-C (TN-C) as a dominant epitope in glioblastoma has been discovered. It is transiently expressed during organogenesis, absent or much reduced in most fully developed organs, but reappears under pathological conditions such as infection, inflammation, or tumorigenesis. It was found that the intensity of TN-C staining correlates with the tumor grade and that the strongest staining indicates poor prognosis.
Therapeutic Ribonucleic Acids in Brain Tumors, 2009
We describe a new, simple and reliable method for diagnosis of a neoplastic disease. It is based ... more We describe a new, simple and reliable method for diagnosis of a neoplastic disease. It is based on the cellulose thin layer chromatography (TLC) quantitative determination of 5-methylcytosine (m5C) in DNA from tumor tissue. Currently, there is a wealth of data showing that oxidative stress effecting through reactive oxygen species (ROS) plays a critical role in the etiology and progression of a number of human diseases. Oxidative damage to DNA, lipids, and proteins is deleterious for the cell. 5-methylcytosine, along with other DNA constituents, is a target for ROS, which results in the appearance of a variety of modified nucleic acid bases. Therefore, m5C moiety constitutes the mutational hotspot; it occurs either within a structural gene or its regulatory regions. Here, we show the results of the analysis of DNA extracted from brain tumor and other tissues. DNA was isolated and sheared by enzymatic digestion into nucleotides which, after labeling with [γ-32p]ATP, were separated on cellulose TLC. A chromatogram was evaluated using phosphoimager and an amount of m5dC was calculated as a ratio (R) of m5dC to m5dC+dC+dT spots intensities. The R values decrease as the malignancy of brain tumors increase. It is the important factor for differentiating low and high grade gliomas. It seems that DNA methylation pattern might be a useful tool for the diagnosis of brain tumors or as a marker for the early detection of the relapse of the disease.
Medical science monitor : international medical journal of experimental and clinical research, 2010
Hypertension is a common disease of the cardiovascular system and one of the main causes of morta... more Hypertension is a common disease of the cardiovascular system and one of the main causes of mortality in the world. Its etiopathogenesis and molecular mechanisms are unknown. Epigenetic changes may play a role in its development. Therefore the level of 5-methylcytosine (5mC), a well-known epigenetic marker, was analyzed in DNA from the blood of essential hypertension patients. TLC chromatographic analysis of the DNA nucleotide composition was used to determine 5mC levels in blood DNA samples from 60 patients suffering from essential hypertension (30 with stage 1 and 30 with stage 2 hypertension) and 30 control subjects. The mean levels of 5mC were 1.80 + or - 0.69 in the healthy subjects, 1.14 + or - 0.48 in all the patients with essential hypertension, 1.29 + or - 0.50 in those with stage 1, and 0.99 + or - 0.42 in those with stage 2 of hypertension. Statistically significant differences in 5mC amount in DNA were observed between the control group and the whole patient group, the c...
The new, simple, and reliable method for the diagnosis of brain tumors is described. It is based ... more The new, simple, and reliable method for the diagnosis of brain tumors is described. It is based on a TLC quantitative determination of 5-methylcytosine (m(5)C) in relation to its damage products of DNA from tumor tissue. Currently, there is evidence that oxidative stress through reactive oxygen species (ROS) plays an important role in the etiology and progression of several human diseases. Oxidative damage of DNA, lipids, and proteins is deleterious for the cell. m(5)C, along with other basic components of DNA, is the target for ROS, which results in the appearance of new modified nucleic acid bases. If so, m(5)C residue constitutes a mutational hotspot position, whether it occurs within a nucleotide sequence of a structural gene or a regulatory region. Here, we show the results of the analysis of 82 DNA samples taken from brain tumor tissues. DNA was isolated and hydrolyzed into nucleotides, which, after labeling with [gamma-(32)P]ATP, were separated on TLC. Chromatograms were eva...
Therapeutic Ribonucleic Acids in Brain Tumors, 2009
Malignant gliomas preferentially express a number of surface markers that may be exploited as the... more Malignant gliomas preferentially express a number of surface markers that may be exploited as therapeutic targets, such as tenascin-C, an extracellular matrix glycoprotein which contributes to tumor cell adhesion, invasion, migration, and proliferation. Glioblastoma ...
Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GB... more Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GBM remains refractory to therapy because of tumor heterogeneity, local invasion, and non-uniform vascular permeability to drugs. Patients with GBM have the median survival of approximately 8-10 months, and for those cases where tumor recurs, the average time of tumor progression after therapy is only eight weeks. A combination of different treatment modes as surgery and chemo- or/and radiotherapy extend survival only for a short time, if any. Recently, tenascin-C (TN-C) as a dominant epitope in glioblastoma has been discovered. It is transiently expressed during organogenesis, absent or much reduced in most fully developed organs, but reappears under pathological conditions such as infection, inflammation, or tumorigenesis. It was found that the intensity of TN-C staining correlates with the tumor grade and that the strongest staining indicates poor prognosis.
We analyze the structure of napin (BngNAP1), a storage protein (mw 14.5 kDa) from Brassica napus.... more We analyze the structure of napin (BngNAP1), a storage protein (mw 14.5 kDa) from Brassica napus. On the basis of the results of 1 H NMR spectroscopy and dynamic light scattering (DLS) studies, the overall shape and secondary structure of the molecule are ...
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Papers by Iwona Gawronska