Bisphenol-A (BPA), a widely used plasticizer, induces cognitive dysfunctions following single and... more Bisphenol-A (BPA), a widely used plasticizer, induces cognitive dysfunctions following single and repeated exposure. Several studies, developed in hippocampus and cortex, tried to find the mechanisms that trigger and mediate these dysfunctions, but those are still not well known. Basal forebrain cholinergic neurons (BFCN) innervate hippocampus and cortex, regulating cognitive function, and their loss or the induction of cholinergic neurotransmission dysfunction leads to cognitive disabilities. However, no studies were performed in BFCN. We treated wild type or histone deacetylase (HDAC2), P75NTR or acetylcholinesterase (AChE) silenced SN56 cholinergic cells from BF with BPA (0.001 μM-100 μM) with or without recombinant nerve growth factor (NGF) and with or without acetylcholine (ACh) for one- and fourteen days in order to elucidate the mechanisms underlying these effects. BPA induced cholinergic neurotransmission disruption through reduction of ChAT activity, and produced apoptotic cell death, mediated partially through AChE-S overexpression and NGF/TrkA/P75NTR signaling dysfunction, independently of cholinergic neurotransmission disruption, following one- and fourteen days of treatment. BPA mediates these alterations, in part, through HDAC2 overexpression. These data are relevant since they may help to elucidate the neurotoxic mechanisms that trigger the cognitive disabilities induced by BPA exposure, providing a new therapeutic approach.
La desconcertante situación de falta de regulación de la profesión del restaurador-conservador of... more La desconcertante situación de falta de regulación de la profesión del restaurador-conservador ofrece un panorama que parece no querer resolverse en perjuicio del patrimonio, que queda desprotegido de intervenciones voluntariosas o lucrativas, y de cuyos perjuicios no se encuentra responsable.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Jan 11, 2018
Cadmium, a neurotoxic environmental compound, produces cognitive disorders, although the mechanis... more Cadmium, a neurotoxic environmental compound, produces cognitive disorders, although the mechanism remains unknown. Cadmium induces a more pronounced cell death on cholinergic neurons from basal forebrain (BF), mediated, in part, by increase in Aβ and total and phosphorylated Tau protein levels, which may explain cadmium effects on learning and memory processes. Cadmium downregulates the expression of heat shock proteins (HSPs) HSP 90, HSP70 and HSP27, and of HSF1, the master regulator of the HSP pathway. HSPs proteins reduce the production of Aβ and phosphorylated Tau proteins and avoid cell death pathways induction. Thus, we hypothesized that cadmium induced the production of Aβ and Tau proteins by HSP pathway disruption through HSF1 expression alteration, leading to BF cholinergic neurons cell death. Our results show that cadmium downregulates HSF1, leading to HSP90, HSP70 and HSP27 gene expression downregulation in BF SN56 cholinergic neurons. In addition, cadmium induced Aβ and...
Cubozoans (box jellyfish) are gonochoristic cnidarians with distinct reproductive strategies. Thi... more Cubozoans (box jellyfish) are gonochoristic cnidarians with distinct reproductive strategies. This comparative histological study examines the gonad organization of Alatina alata and Copula sivickisi, two box jellyfish species that exhibit different modes of internal fertilization. A. alata reproduces via spermcasting aggregations while C. sivickisi reproduces via copulation; in both cases, internal fertilization occurs in the gastrovascular cavity. Herein, we provide the first histological description of subgastric sacs-structures unique to C. sivickisi. Although previously thought to function as sperm storage sacs, our findings reveal that subgastric sacs are nematocyst nests lacking sperm entirely. Conversely, we discovered that velarial spots in C. sivickisi females correspond to actual sperm storage structures. Histological examination of cubozoan sperm packages revealed that while sperm packages from both species have motile flagella, A. alata males produce nonencapsulated spe...
Chlorpyrifos (CPF) is an organophosphates insecticide reported to induce, both after acute and re... more Chlorpyrifos (CPF) is an organophosphates insecticide reported to induce, both after acute and repeated exposure, cognitive disorders and basal forebrain cholinergic neuronal loss, involved on learning and memory regulation, which could be the cause of such cognitive disorders. This neuronal loss was mediated partially by AChE variants alteration, suggesting other mechanisms are involved. In this regard, CPF induces oxidative stress that is implicated in the induction of cognitive deficits, changes in AChE variants expression and neuronal loss. Otherwise, it has been shown that P75(NTR) and the α7-nAChRs expression is altered in basal forebrain of rats after CPF long-term exposure; this alteration has been related with oxidative stress induction, cholinergic cell loss, and disruption of learning and memory processes. According to these data, we hypothesized that CPF induces basal forebrain cholinergic neuronal loss through induction of oxidative stress produced by P75(NTR) and α7-nA...
La museologia es uno de los usos que se pueden dar al patrimonio industrial edificado como proyec... more La museologia es uno de los usos que se pueden dar al patrimonio industrial edificado como proyecto de conservacion, pero el caso particular de este tipo de patrimonio presenta peculiaridades que no siempre se evidencian con una musealizacion tradicional. La museologia y la museografia han ido evolucionando desde su nacimiento para llegar a la muestra y comprension de todo tipo de patrimonio por parte del mas amplio espectro de publico. En este articulo se explica como las nuevas tendencias museologicas y museograficas consiguen este objetivo especialmente en el patrimonio industrial, y a traves del analisis de casos reales en Europa se pueden ver gestiones de exito de un patrimonio industrial especifico: el molino-fabrica de papel.
Chlorpyrifos (CPF) is one of the most widely used organophosphates insecticides that has been rep... more Chlorpyrifos (CPF) is one of the most widely used organophosphates insecticides that has been reported to induce cognitive disorders both after acute and repeated administration similar to those induced in Alzheimer's disease (AD). However, the mechanisms through which it induces these effects are unknown. On the other hand, the cholinergic system, mainly basal forebrain cholinergic neurons, is involved in learning and memory regulation, and an alteration of cholinergic transmission or/and cholinergic cell loss could induce these effects. In this regard, it has been reported that CPF can affect cholinergic transmission, and alter AChE variants, which have been shown to be related with basal forebrain cholinergic neuronal loss. According…
A procedure for the determination of active components in insecticide formulations showing overla... more A procedure for the determination of active components in insecticide formulations showing overlapped peaks in liquid chromatography has been devised. The procedure is based in the use of derivative spectra of the components obtained by a diode-array spectrophotometer around the maxima signal of the chromatographic peak, and has been applied to the analysis of mixtures of piperonyl butoxide, neopynamine and
Bisphenol-A (BPA), a widely used plasticizer, induces cognitive dysfunctions following single and... more Bisphenol-A (BPA), a widely used plasticizer, induces cognitive dysfunctions following single and repeated exposure. Several studies, developed in hippocampus and cortex, tried to find the mechanisms that trigger and mediate these dysfunctions, but those are still not well known. Basal forebrain cholinergic neurons (BFCN) innervate hippocampus and cortex, regulating cognitive function, and their loss or the induction of cholinergic neurotransmission dysfunction leads to cognitive disabilities. However, no studies were performed in BFCN. We treated wild type or histone deacetylase (HDAC2), P75NTR or acetylcholinesterase (AChE) silenced SN56 cholinergic cells from BF with BPA (0.001 μM-100 μM) with or without recombinant nerve growth factor (NGF) and with or without acetylcholine (ACh) for one- and fourteen days in order to elucidate the mechanisms underlying these effects. BPA induced cholinergic neurotransmission disruption through reduction of ChAT activity, and produced apoptotic cell death, mediated partially through AChE-S overexpression and NGF/TrkA/P75NTR signaling dysfunction, independently of cholinergic neurotransmission disruption, following one- and fourteen days of treatment. BPA mediates these alterations, in part, through HDAC2 overexpression. These data are relevant since they may help to elucidate the neurotoxic mechanisms that trigger the cognitive disabilities induced by BPA exposure, providing a new therapeutic approach.
La desconcertante situación de falta de regulación de la profesión del restaurador-conservador of... more La desconcertante situación de falta de regulación de la profesión del restaurador-conservador ofrece un panorama que parece no querer resolverse en perjuicio del patrimonio, que queda desprotegido de intervenciones voluntariosas o lucrativas, y de cuyos perjuicios no se encuentra responsable.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Jan 11, 2018
Cadmium, a neurotoxic environmental compound, produces cognitive disorders, although the mechanis... more Cadmium, a neurotoxic environmental compound, produces cognitive disorders, although the mechanism remains unknown. Cadmium induces a more pronounced cell death on cholinergic neurons from basal forebrain (BF), mediated, in part, by increase in Aβ and total and phosphorylated Tau protein levels, which may explain cadmium effects on learning and memory processes. Cadmium downregulates the expression of heat shock proteins (HSPs) HSP 90, HSP70 and HSP27, and of HSF1, the master regulator of the HSP pathway. HSPs proteins reduce the production of Aβ and phosphorylated Tau proteins and avoid cell death pathways induction. Thus, we hypothesized that cadmium induced the production of Aβ and Tau proteins by HSP pathway disruption through HSF1 expression alteration, leading to BF cholinergic neurons cell death. Our results show that cadmium downregulates HSF1, leading to HSP90, HSP70 and HSP27 gene expression downregulation in BF SN56 cholinergic neurons. In addition, cadmium induced Aβ and...
Cubozoans (box jellyfish) are gonochoristic cnidarians with distinct reproductive strategies. Thi... more Cubozoans (box jellyfish) are gonochoristic cnidarians with distinct reproductive strategies. This comparative histological study examines the gonad organization of Alatina alata and Copula sivickisi, two box jellyfish species that exhibit different modes of internal fertilization. A. alata reproduces via spermcasting aggregations while C. sivickisi reproduces via copulation; in both cases, internal fertilization occurs in the gastrovascular cavity. Herein, we provide the first histological description of subgastric sacs-structures unique to C. sivickisi. Although previously thought to function as sperm storage sacs, our findings reveal that subgastric sacs are nematocyst nests lacking sperm entirely. Conversely, we discovered that velarial spots in C. sivickisi females correspond to actual sperm storage structures. Histological examination of cubozoan sperm packages revealed that while sperm packages from both species have motile flagella, A. alata males produce nonencapsulated spe...
Chlorpyrifos (CPF) is an organophosphates insecticide reported to induce, both after acute and re... more Chlorpyrifos (CPF) is an organophosphates insecticide reported to induce, both after acute and repeated exposure, cognitive disorders and basal forebrain cholinergic neuronal loss, involved on learning and memory regulation, which could be the cause of such cognitive disorders. This neuronal loss was mediated partially by AChE variants alteration, suggesting other mechanisms are involved. In this regard, CPF induces oxidative stress that is implicated in the induction of cognitive deficits, changes in AChE variants expression and neuronal loss. Otherwise, it has been shown that P75(NTR) and the α7-nAChRs expression is altered in basal forebrain of rats after CPF long-term exposure; this alteration has been related with oxidative stress induction, cholinergic cell loss, and disruption of learning and memory processes. According to these data, we hypothesized that CPF induces basal forebrain cholinergic neuronal loss through induction of oxidative stress produced by P75(NTR) and α7-nA...
La museologia es uno de los usos que se pueden dar al patrimonio industrial edificado como proyec... more La museologia es uno de los usos que se pueden dar al patrimonio industrial edificado como proyecto de conservacion, pero el caso particular de este tipo de patrimonio presenta peculiaridades que no siempre se evidencian con una musealizacion tradicional. La museologia y la museografia han ido evolucionando desde su nacimiento para llegar a la muestra y comprension de todo tipo de patrimonio por parte del mas amplio espectro de publico. En este articulo se explica como las nuevas tendencias museologicas y museograficas consiguen este objetivo especialmente en el patrimonio industrial, y a traves del analisis de casos reales en Europa se pueden ver gestiones de exito de un patrimonio industrial especifico: el molino-fabrica de papel.
Chlorpyrifos (CPF) is one of the most widely used organophosphates insecticides that has been rep... more Chlorpyrifos (CPF) is one of the most widely used organophosphates insecticides that has been reported to induce cognitive disorders both after acute and repeated administration similar to those induced in Alzheimer's disease (AD). However, the mechanisms through which it induces these effects are unknown. On the other hand, the cholinergic system, mainly basal forebrain cholinergic neurons, is involved in learning and memory regulation, and an alteration of cholinergic transmission or/and cholinergic cell loss could induce these effects. In this regard, it has been reported that CPF can affect cholinergic transmission, and alter AChE variants, which have been shown to be related with basal forebrain cholinergic neuronal loss. According…
A procedure for the determination of active components in insecticide formulations showing overla... more A procedure for the determination of active components in insecticide formulations showing overlapped peaks in liquid chromatography has been devised. The procedure is based in the use of derivative spectra of the components obtained by a diode-array spectrophotometer around the maxima signal of the chromatographic peak, and has been applied to the analysis of mixtures of piperonyl butoxide, neopynamine and
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