Horse chestnut fruits and seeds have been traditionally used as a remedy against chronic venous i... more Horse chestnut fruits and seeds have been traditionally used as a remedy against chronic venous insuf-ficiency symptoms. Currently the total extracts or purified saponins are extensively used as dietary supplements or medicines' ingredients in numerous authorized pharmaceuticals. The goal of this study was to investigate the changes in the secondary metabolites content within time. The selected optimal conditions for collection could provide the highest quantity of active compounds in the extracts, which will increase the quality of the final product. The obtained results indicate that the highest content of natural products in the horse chestnut fruits was measured between the 13th and 16th week from the beginning of blooming. The quantitative analysis of escin and two flavonoids: kaempferol and procyani-din A2 was performed on the extracts within the study. The content of the selected natural products increased of ca. 90% between the 12th and 20th week for escin and of ca. 120% between the 7th and 12th week, for flavonoids. The rise in natural products quantity was also expressed in the measured antioxidant activity and total phenolic content values in DPPH and Folin–Ciocalteu tests.
The presence of phenolic content in overground extracts of Euonymus verruco-sus Scop. – commonly ... more The presence of phenolic content in overground extracts of Euonymus verruco-sus Scop. – commonly growing in Europe – has been reported recently. The chroma-tographical and spectral data revealed the presence of several simple phenolic acids (gal-lic, protocatechuic, p-hydroxybenzoic, vanillic, syringic, caffeic, p-coumaric, ferulic, and m-coumaric acids), both as free and conjugated with other secondary metabolites. The comparison of two-dimensional TLC systems on cellulose stationary phases with HPLC– DAD reversed-phase chromatography was performed to assess a cheap and rapid technique in the identification process of major phenolic constituents. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging tests, expressed as IC 50 , revealed the most beneficial results for the fraction after alkaline hydrolysis and yielded 205 ± 8 μg mL −1 .
Eight new coumarins were isolated from the fruits of Seseli devenyense Simonkai. Their structures... more Eight new coumarins were isolated from the fruits of Seseli devenyense Simonkai. Their structures were established from NMR and mass data and their absolute configurations from chemical degradation correlation reactions. The new structures are the decanoic and dodecanoic esters of (+)-lomatin (3, 4), the decanoates of (+)-cis-khellactone at positions 4' (5) and 3' (6) as well as the 2'S epimer of 8-(2,3-dihydroxy-3-methylbutyl)-7-hydroxychromen-2-one (7) named devenyol, its two O-monoglucosides at positions 3' and 7 named devenyosides A (8) and B (9), and the corresponding 3'- and 7-O-diglucoside named devenyoside C (10). This plant is an interesting example of stereochemical diversity based on biodiversity given that other members of the Apiaceae family produce exclusively the 2'R epimers of compounds 7-9.
In this paper, comparison of the volatile components composition in the samples obtained by hydro... more In this paper, comparison of the volatile components composition in the samples obtained by hydrodistillation and solid-phase microextraction of Salvia officinalis was described. Different sample preparation techniques showed considerable differences in volatiles composition, especially with respect to sesqui- and diterpenoids. The comparison of the sage essential oil obtained by hydrodistillation in the Deryng and Clevenger type apparatus, according to the pharmacopoeial methods (FP VI and VII), showed the presence of the same terpenoids in both essential oils, however, the relative percentage composition of the components were different. These differences are caused by the different extraction times used in both methods. Since each essential oil to be admitted to medicinal use should meet requirements regarding the composition of major chemical components, the minimum time for the hydrodistillation of the essential oils from sage should be 1 h.
The current study was performed to examine the qualitative and quantitative composition of the es... more The current study was performed to examine the qualitative and quantitative composition of the essential oil, n-hexane and ethanol extracts from the rhizomes of Iris dichotoma Pall. for their volatile content. The essential oil was particularly found to possess the most rich composition, while the latter extracts (n-hexane and ethanolic ones) consisted of various fatty acid esters. GC and GC-MS analyses of the oil resulted in the identification of 28 components. Among them myristic acid (13.67 %), methyl myristate (9.08 %), and α-terpineol (2.42 %) were found to be the dominant compounds. The qualitative composition of both n-hexane and ethanol extracts of Iris dichotoma Pall., obtained by pressurized liquid extraction, was similar and revealed the presence of 6 components, which were identified as natural products with methyl myristate as the major one (22.47 % and 10.18 % in ethanolic and n-hexane extracts, respectively)
Horse chestnut fruits and seeds have been traditionally used as a remedy against chronic venous i... more Horse chestnut fruits and seeds have been traditionally used as a remedy against chronic venous insuf-ficiency symptoms. Currently the total extracts or purified saponins are extensively used as dietary supplements or medicines' ingredients in numerous authorized pharmaceuticals. The goal of this study was to investigate the changes in the secondary metabolites content within time. The selected optimal conditions for collection could provide the highest quantity of active compounds in the extracts, which will increase the quality of the final product. The obtained results indicate that the highest content of natural products in the horse chestnut fruits was measured between the 13th and 16th week from the beginning of blooming. The quantitative analysis of escin and two flavonoids: kaempferol and procyani-din A2 was performed on the extracts within the study. The content of the selected natural products increased of ca. 90% between the 12th and 20th week for escin and of ca. 120% between the 7th and 12th week, for flavonoids. The rise in natural products quantity was also expressed in the measured antioxidant activity and total phenolic content values in DPPH and Folin–Ciocalteu tests.
The presence of phenolic content in overground extracts of Euonymus verruco-sus Scop. – commonly ... more The presence of phenolic content in overground extracts of Euonymus verruco-sus Scop. – commonly growing in Europe – has been reported recently. The chroma-tographical and spectral data revealed the presence of several simple phenolic acids (gal-lic, protocatechuic, p-hydroxybenzoic, vanillic, syringic, caffeic, p-coumaric, ferulic, and m-coumaric acids), both as free and conjugated with other secondary metabolites. The comparison of two-dimensional TLC systems on cellulose stationary phases with HPLC– DAD reversed-phase chromatography was performed to assess a cheap and rapid technique in the identification process of major phenolic constituents. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging tests, expressed as IC 50 , revealed the most beneficial results for the fraction after alkaline hydrolysis and yielded 205 ± 8 μg mL −1 .
Eight new coumarins were isolated from the fruits of Seseli devenyense Simonkai. Their structures... more Eight new coumarins were isolated from the fruits of Seseli devenyense Simonkai. Their structures were established from NMR and mass data and their absolute configurations from chemical degradation correlation reactions. The new structures are the decanoic and dodecanoic esters of (+)-lomatin (3, 4), the decanoates of (+)-cis-khellactone at positions 4' (5) and 3' (6) as well as the 2'S epimer of 8-(2,3-dihydroxy-3-methylbutyl)-7-hydroxychromen-2-one (7) named devenyol, its two O-monoglucosides at positions 3' and 7 named devenyosides A (8) and B (9), and the corresponding 3'- and 7-O-diglucoside named devenyoside C (10). This plant is an interesting example of stereochemical diversity based on biodiversity given that other members of the Apiaceae family produce exclusively the 2'R epimers of compounds 7-9.
In this paper, comparison of the volatile components composition in the samples obtained by hydro... more In this paper, comparison of the volatile components composition in the samples obtained by hydrodistillation and solid-phase microextraction of Salvia officinalis was described. Different sample preparation techniques showed considerable differences in volatiles composition, especially with respect to sesqui- and diterpenoids. The comparison of the sage essential oil obtained by hydrodistillation in the Deryng and Clevenger type apparatus, according to the pharmacopoeial methods (FP VI and VII), showed the presence of the same terpenoids in both essential oils, however, the relative percentage composition of the components were different. These differences are caused by the different extraction times used in both methods. Since each essential oil to be admitted to medicinal use should meet requirements regarding the composition of major chemical components, the minimum time for the hydrodistillation of the essential oils from sage should be 1 h.
The current study was performed to examine the qualitative and quantitative composition of the es... more The current study was performed to examine the qualitative and quantitative composition of the essential oil, n-hexane and ethanol extracts from the rhizomes of Iris dichotoma Pall. for their volatile content. The essential oil was particularly found to possess the most rich composition, while the latter extracts (n-hexane and ethanolic ones) consisted of various fatty acid esters. GC and GC-MS analyses of the oil resulted in the identification of 28 components. Among them myristic acid (13.67 %), methyl myristate (9.08 %), and α-terpineol (2.42 %) were found to be the dominant compounds. The qualitative composition of both n-hexane and ethanol extracts of Iris dichotoma Pall., obtained by pressurized liquid extraction, was similar and revealed the presence of 6 components, which were identified as natural products with methyl myristate as the major one (22.47 % and 10.18 % in ethanolic and n-hexane extracts, respectively)
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