There is a great need for acellular, fully vascularized, and biocompatible myocardial scaffolds t... more There is a great need for acellular, fully vascularized, and biocompatible myocardial scaffolds that provide an agreeable biological, nutritional, and biomechanical "niches" for reseeded cells for in vitro and in vivo applications. We generated myocardial flap scaffolds comprising porcine left-anterior ventricular myocardium and its associated coronary arteries and veins and investigated the combinatorial effects of sodium dodecyl sulfate (SDS) and sodium hydroxide (NaOH) perfusion on both the myocardial extracellular matrix (ECM) and the vascular ECM. Results showed that all scaffolds displayed a fully intact and patent vasculature; with arterial burst pressures indistinguishable from native coronary arteries and perfusion to the level of capillaries. Scaffolds were free of cellular proteins and retained collagen and elastin ECM components, exhibited excellent mechanical properties, and were cytocompatible towards relevant seeded cells. SDS perfusion preserved collagen IV...
There is a great need for acellular, fully vascularized, and biocompatible myocardial scaffolds t... more There is a great need for acellular, fully vascularized, and biocompatible myocardial scaffolds that provide an agreeable biological, nutritional, and biomechanical "niches" for reseeded cells for in vitro and in vivo applications. We generated myocardial flap scaffolds comprising porcine left-anterior ventricular myocardium and its associated coronary arteries and veins and investigated the combinatorial effects of sodium dodecyl sulfate (SDS) and sodium hydroxide (NaOH) perfusion on both the myocardial extracellular matrix (ECM) and the vascular ECM. Results showed that all scaffolds displayed a fully intact and patent vasculature; with arterial burst pressures indistinguishable from native coronary arteries and perfusion to the level of capillaries. Scaffolds were free of cellular proteins and retained collagen and elastin ECM components, exhibited excellent mechanical properties, and were cytocompatible towards relevant seeded cells. SDS perfusion preserved collagen IV...
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