Plants are attacked, not only by various microorganisms, but also by other enemies, such as mollu... more Plants are attacked, not only by various microorganisms, but also by other enemies, such as molluscs, nematods, mites, and insects. They have evolved complex and efficient mechanisms to defend themselves against pathogens (hypersensitive response, systemic acquired resistance) and herbivores (release of volatile compounds that attract predators of the herbivores, accumulation of proteinase inhibitors). Yet, the confrontation of the plants with their invaders can also turn to the advantage of the latter. In the past, the attacks of crops regularly brought about dramatic economic losses. From the World War II onwards, the development of organic chemistry associated with a growing awareness of the problems of agriculture has resulted in the production of a constantly growing number of plant protection products. They are currently divided into about ten classes, the herbicides, fungicides, and insecticides-acaricides making up more than 90% of the world market. Most of the agrochemical ...
Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa... more Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa pudica L., and transmembrane potentials were measured on cortical cells. In comparison with the nonspecialized cortical cells of the petiole or stem, the proton pump was highly expressed in motor cells. These immunological data are in close agreement with electrophysiological data, because the active component of the transmembrane potential was low in the nonspecialized cortical cells and high in motor cells. Therefore, motor cells contain the plasma membrane H+-ATPase required to mediate the ionic fluxes that are involved in circadian leaf movements and that are necessary to recover the turgor potential that is considerably affected by the large K+ and Cl- efflux associated with seismonastic movement. With the exception of sieve tubes, the phloem also had a high density of H+-ATPase. This suggests that the recovery of the transmembrane ionic gradients (K+ and Cl-), which is affected by...
... Sabine Bouch~-Pillon l, Pierrette Fleurat-Lessard j, Ramon Serrano 2, Jean-Louis Bonnemain 1 ... more ... Sabine Bouch~-Pillon l, Pierrette Fleurat-Lessard j, Ramon Serrano 2, Jean-Louis Bonnemain 1 Laboratoire de Physiologie et Biochimie V6g~tales ... specialization which increases the surface area of the plasma membrane from 3 to 10 times (for reviews, see Pate and Gunning ...
Owing to the complexity of soil composition, accurate predictions of both apoplastic systemicity ... more Owing to the complexity of soil composition, accurate predictions of both apoplastic systemicity of lipophilic xenobiotics and their leaching from the soil are made difficult. Therefore, a non-destructive method to assess directly these two components of the spatial behaviour of soil-applied phytochemicals is needed. The plant selected was a dwarf tomato, which can exude an abundant apoplastic fluid through large stomata for several months. The feasibility and reliability of the method were assayed using three triazoles exhibiting different log D values. HPLC-MS analyses indicated that triadimenol (log D = 2.97) was clearly the most mobile compound within the apoplast, especially its diastereoisomer A. Propiconazole (log D = 3.65) and penconazole (log D = 4.64) exhibited a similar low systemicity. The data remained the same when the three fungicides were applied together on the soil. Long time-course studies (1.5 months) of penconazole behaviour indicated that, in contrast to leaching, which decrease sharply, root-to-shoot translocation remained almost unchanged during the whole experiment, in spite of the high lipophilicity of this fungicide. This method must contribute to a better knowledge of the behaviour of commercial soil-applied phytochemicals. It can also be used to screen new xenobiotics within strategies to satisfy environmental requirements.
Plants are attacked, not only by various microorganisms, but also by other enemies, such as mollu... more Plants are attacked, not only by various microorganisms, but also by other enemies, such as molluscs, nematods, mites, and insects. They have evolved complex and efficient mechanisms to defend themselves against pathogens (hypersensitive response, systemic acquired resistance) and herbivores (release of volatile compounds that attract predators of the herbivores, accumulation of proteinase inhibitors). Yet, the confrontation of the plants with their invaders can also turn to the advantage of the latter. In the past, the attacks of crops regularly brought about dramatic economic losses. From the World War II onwards, the development of organic chemistry associated with a growing awareness of the problems of agriculture has resulted in the production of a constantly growing number of plant protection products. They are currently divided into about ten classes, the herbicides, fungicides, and insecticides-acaricides making up more than 90% of the world market. Most of the agrochemical ...
Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa... more Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa pudica L., and transmembrane potentials were measured on cortical cells. In comparison with the nonspecialized cortical cells of the petiole or stem, the proton pump was highly expressed in motor cells. These immunological data are in close agreement with electrophysiological data, because the active component of the transmembrane potential was low in the nonspecialized cortical cells and high in motor cells. Therefore, motor cells contain the plasma membrane H+-ATPase required to mediate the ionic fluxes that are involved in circadian leaf movements and that are necessary to recover the turgor potential that is considerably affected by the large K+ and Cl- efflux associated with seismonastic movement. With the exception of sieve tubes, the phloem also had a high density of H+-ATPase. This suggests that the recovery of the transmembrane ionic gradients (K+ and Cl-), which is affected by...
... Sabine Bouch~-Pillon l, Pierrette Fleurat-Lessard j, Ramon Serrano 2, Jean-Louis Bonnemain 1 ... more ... Sabine Bouch~-Pillon l, Pierrette Fleurat-Lessard j, Ramon Serrano 2, Jean-Louis Bonnemain 1 Laboratoire de Physiologie et Biochimie V6g~tales ... specialization which increases the surface area of the plasma membrane from 3 to 10 times (for reviews, see Pate and Gunning ...
Owing to the complexity of soil composition, accurate predictions of both apoplastic systemicity ... more Owing to the complexity of soil composition, accurate predictions of both apoplastic systemicity of lipophilic xenobiotics and their leaching from the soil are made difficult. Therefore, a non-destructive method to assess directly these two components of the spatial behaviour of soil-applied phytochemicals is needed. The plant selected was a dwarf tomato, which can exude an abundant apoplastic fluid through large stomata for several months. The feasibility and reliability of the method were assayed using three triazoles exhibiting different log D values. HPLC-MS analyses indicated that triadimenol (log D = 2.97) was clearly the most mobile compound within the apoplast, especially its diastereoisomer A. Propiconazole (log D = 3.65) and penconazole (log D = 4.64) exhibited a similar low systemicity. The data remained the same when the three fungicides were applied together on the soil. Long time-course studies (1.5 months) of penconazole behaviour indicated that, in contrast to leaching, which decrease sharply, root-to-shoot translocation remained almost unchanged during the whole experiment, in spite of the high lipophilicity of this fungicide. This method must contribute to a better knowledge of the behaviour of commercial soil-applied phytochemicals. It can also be used to screen new xenobiotics within strategies to satisfy environmental requirements.
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