Polybrominated diphenyl ethers (PBDEs) industrially used as flame retardants are nowadays conside... more Polybrominated diphenyl ethers (PBDEs) industrially used as flame retardants are nowadays considered emerging pollutants as they are endocrine disrupting chemicals (EDCs), persistent in the environment, bioaccumulative and in addition, its hydroxylated (OH-BDEs) and methoxylated (MeO-BDEs) metabolites have similar ecotoxic properties. The aim of this work was to develop an analytical method to be applied in the study of the bioconcentration and biotransformation of BDE-47 due to its bioavailability, toxicity and high persistence and abundance in environmental samples, including humans. So, a dependable ultrasonic extraction process followed to dispersive SPE clean-up step and GC-MS-μECD detection has worked out for the determination of BDE-47 and its main biotransformation products (MeO-BDEs and OH-BDEs), considering the polarity difference. In addition, an alternative method to bioconcentration official guideline OECD 305, developed previously with zebrafish (Danio rerio) eleuthero...
Antidepressant drugs are widely used for the treatment of common mental or other psychiatric diso... more Antidepressant drugs are widely used for the treatment of common mental or other psychiatric disorders such as depression, which affect about 121 million people worldwide. This widespread use has contributed to the input of these pharmaceuticals and their metabolites into the environment. The aim of this work was to develop an analytical method to quantify the most widely used antidepressant drugs, selective serotonin reuptake inhibitors (SSRI), and their main metabolites in the environment. For this, a new and reliable miniaturized extraction method based on dispersive SPE cleanup procedure for extraction of SSRI followed by derivatization with n-heptafluorobutyrylimidazole, and detection by GC-MS was developed. The methodology, including a first-order one-compartment model, was then applied to a bioconcentration study in zebrafish (Danio rerio) eleutheroembryos. The results showed low bioaccumulation of these compounds; however, a biotransformation evidence of the parent compounds...
Este CIUR incluye cuatro aportaciones: En la primera Marian Simón y Jon Sanz plantean cómo para l... more Este CIUR incluye cuatro aportaciones: En la primera Marian Simón y Jon Sanz plantean cómo para los movimientos por la soberanía alimentaria, sería estratégico incidir en los mecanismos de ordenación y planificación espacial, desde los que forzar la reorganización del sistema de producción y consumo. En esa línea, Luis Alberto Bermejo y Fátima Cubas explican los conflictos derivados de los planes de espacios protegidos, que restringen usos tradicionales, las dificultades del pastoreo en zonas fragmentadas por la expansión urbana en La Orotava (Tenerife) y la experiencia de la mediación institucional para nuevos planes de ordenación. Desde otra perspectiva y mirando a las luchas alimentarias en las periferias urbanas en Lisboa (Portugal) Inés Morales reflexiona sobre las contradicciones de los planes de urbanización y las estrategias de los movimientos alimentarios y la contribución de ambos a los actuales procesos de…
In the aftermath of the economic crisis in the city of Madrid, food geography transformed. The ur... more In the aftermath of the economic crisis in the city of Madrid, food geography transformed. The urban unemployed began to engage in agriculture in periurban areas, creating new alliances between producers and consumers. Over a period of 15 years the alternative food movement organized on the fringe gave way to agroecological civic platforms that are highly assertive, and a dialogue with political institutions has opened. A key moment in the advance of this proactive attitude came about in the municipal elections of May 2015. Activists ascended to positions of political power and the backdrop of the Milan Urban Food Policy Pact, created an opportunity for the food movement to move from protest to program, and public policies permeated by agroecological principles.
Exposure to heavy metals has represented one of the most serious health risks of environmental po... more Exposure to heavy metals has represented one of the most serious health risks of environmental pollution over the last 50 years. Most of the bioconcentration studies that have been carried out to date explored only individual contaminants, unlike the real situations that occur in the environment. In this work, zebrafish eleutheroembryos were exposed to a mixture of CH3Hg(II), iAs(III), Ag(I) and Cd(II), and new BCFs were calculated and compared with those calculated from single metal exposures. In both cases, experimental conditions meet the OECD Test 305 conditions established for aquatic systems. In addition, spatial imaging obtained by laser ablation coupled to inductively plasma mass spectrometry (LA-ICP/MS), has been directly performed in these samples providing complementary information. The new BCF's have revealed some differences compared to single metal exposures when eleutheroembryos were exposed to the metal mixture, especially for iAs(III) and Cd(II). LA-ICP/MS image...
In this study we evaluated the exposure effects of mixtures of five polycyclic aromatic hydrocarb... more In this study we evaluated the exposure effects of mixtures of five polycyclic aromatic hydrocarbons (PAHs); namely, benzo[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene and chrysene on zebrafish embryos. Supplementation of the exposure media with 0.45% dimethyl sulfoxide and 50 ppm of Tween 20 could guarantee the solubilization and stabilization of the PAHs up to 24 h without affecting the embryos development. The exposure effects were tested by detecting the differential expression of a number of genes related to the aryl hydrocarbon receptor gene battery. Effects were detectable already after 6 h of exposure. After 24 h of exposure, all PAHs, except for benzo[a]anthracene, acted as potent inducers of the gene cyp1a1. Benzo[k]fluoranthene was the major inducer; the effect caused by the mixture at the lower concentration tested (1 ng ml(-1) ) was dominated by its presence. However, in the mixture at the highest concentration tested (10 ng ml(-1) ) it cause...
The concern related to the use of nanomaterials is growing nowadays, especially the risk associat... more The concern related to the use of nanomaterials is growing nowadays, especially the risk associated with their emission or exposure. One type of nanomaterials that has attracted much attention is quantum dots (QDs). QDs incorporation in consumer goods increases the probability of their entering in the environment and then into living organisms and human. In order to evaluate their potential to be bioconcentrated, zebrafish larvae have been exposed to SeCd/ZnS QDs, after performing an exhaustive characterization of these nanoparticles under the assay conditions. These data were compared with those obtained when zebrafish larvae were exposed to ionic cadmium. Finally, distribution of ionic Cd and QDs in exposed zebrafish larvae have been evaluated by Laser Ablation ICP-MS.
The European REACH legislation establishes the need to study the toxicity, persistence and bioacc... more The European REACH legislation establishes the need to study the toxicity, persistence and bioaccumulation of those chemicals with an exceeding production of 100tons and/or chemicals considered PBTs substances (Persistence, Bioaccumulation and Toxicity). Currently, the OECD technical guideline 305 is the most used protocol to determine bioconcentration factors of contaminants in aquatic environments. However, this procedure implies high cost and amount of adult fishes. Zebrafish (Danio Rerio) has been selected since this animal model has several advantageous features over other vertebrates, mainly fast embryonic development and easy growth. The analytical methodology here developed has been applied to calculate the bioconcentration factors (BCFs) of two contaminants: inorganic arsenic and tributyltin (measured as arsenic and tin). The method is based on the use of an ultrasonic probe assisted extraction for accelerating the sample treatment followed by detection using graphite furnace atomic absorption spectrometry with Zeeman correction (ZGFAAS). Results obtained for the BCFs values are in good agreement with previously reported data on freshwater aquatic organisms. In the case of arsenic, after exposing larvae to concentrations of 5 and 50μgL(-1), very low BCFs were observed (between 2.2 and 9.5); while for tributyltin, the BCFs observed were within the range 840-1280 after exposure to concentrations of 0.2 and 2.0μgL(-1), respectively. This study shows the use of zebrafish larvae together with the proposed analytical approach as a promising alternative to the OECD 305 test to evaluate the BCFs of classical and emergent contaminants.
This study examined the effects of arsenic exposure and the biotransformation capacity of develop... more This study examined the effects of arsenic exposure and the biotransformation capacity of developing Iberian green frogs (Rana perezi) with the main aim of finding valid biomarkers to monitor pollution and toxicological problems. Total arsenic concentration and arsenic ...
Polybrominated diphenyl ethers (PBDEs) industrially used as flame retardants are nowadays conside... more Polybrominated diphenyl ethers (PBDEs) industrially used as flame retardants are nowadays considered emerging pollutants as they are endocrine disrupting chemicals (EDCs), persistent in the environment, bioaccumulative and in addition, its hydroxylated (OH-BDEs) and methoxylated (MeO-BDEs) metabolites have similar ecotoxic properties. The aim of this work was to develop an analytical method to be applied in the study of the bioconcentration and biotransformation of BDE-47 due to its bioavailability, toxicity and high persistence and abundance in environmental samples, including humans. So, a dependable ultrasonic extraction process followed to dispersive SPE clean-up step and GC-MS-μECD detection has worked out for the determination of BDE-47 and its main biotransformation products (MeO-BDEs and OH-BDEs), considering the polarity difference. In addition, an alternative method to bioconcentration official guideline OECD 305, developed previously with zebrafish (Danio rerio) eleuthero...
Antidepressant drugs are widely used for the treatment of common mental or other psychiatric diso... more Antidepressant drugs are widely used for the treatment of common mental or other psychiatric disorders such as depression, which affect about 121 million people worldwide. This widespread use has contributed to the input of these pharmaceuticals and their metabolites into the environment. The aim of this work was to develop an analytical method to quantify the most widely used antidepressant drugs, selective serotonin reuptake inhibitors (SSRI), and their main metabolites in the environment. For this, a new and reliable miniaturized extraction method based on dispersive SPE cleanup procedure for extraction of SSRI followed by derivatization with n-heptafluorobutyrylimidazole, and detection by GC-MS was developed. The methodology, including a first-order one-compartment model, was then applied to a bioconcentration study in zebrafish (Danio rerio) eleutheroembryos. The results showed low bioaccumulation of these compounds; however, a biotransformation evidence of the parent compounds...
Este CIUR incluye cuatro aportaciones: En la primera Marian Simón y Jon Sanz plantean cómo para l... more Este CIUR incluye cuatro aportaciones: En la primera Marian Simón y Jon Sanz plantean cómo para los movimientos por la soberanía alimentaria, sería estratégico incidir en los mecanismos de ordenación y planificación espacial, desde los que forzar la reorganización del sistema de producción y consumo. En esa línea, Luis Alberto Bermejo y Fátima Cubas explican los conflictos derivados de los planes de espacios protegidos, que restringen usos tradicionales, las dificultades del pastoreo en zonas fragmentadas por la expansión urbana en La Orotava (Tenerife) y la experiencia de la mediación institucional para nuevos planes de ordenación. Desde otra perspectiva y mirando a las luchas alimentarias en las periferias urbanas en Lisboa (Portugal) Inés Morales reflexiona sobre las contradicciones de los planes de urbanización y las estrategias de los movimientos alimentarios y la contribución de ambos a los actuales procesos de…
In the aftermath of the economic crisis in the city of Madrid, food geography transformed. The ur... more In the aftermath of the economic crisis in the city of Madrid, food geography transformed. The urban unemployed began to engage in agriculture in periurban areas, creating new alliances between producers and consumers. Over a period of 15 years the alternative food movement organized on the fringe gave way to agroecological civic platforms that are highly assertive, and a dialogue with political institutions has opened. A key moment in the advance of this proactive attitude came about in the municipal elections of May 2015. Activists ascended to positions of political power and the backdrop of the Milan Urban Food Policy Pact, created an opportunity for the food movement to move from protest to program, and public policies permeated by agroecological principles.
Exposure to heavy metals has represented one of the most serious health risks of environmental po... more Exposure to heavy metals has represented one of the most serious health risks of environmental pollution over the last 50 years. Most of the bioconcentration studies that have been carried out to date explored only individual contaminants, unlike the real situations that occur in the environment. In this work, zebrafish eleutheroembryos were exposed to a mixture of CH3Hg(II), iAs(III), Ag(I) and Cd(II), and new BCFs were calculated and compared with those calculated from single metal exposures. In both cases, experimental conditions meet the OECD Test 305 conditions established for aquatic systems. In addition, spatial imaging obtained by laser ablation coupled to inductively plasma mass spectrometry (LA-ICP/MS), has been directly performed in these samples providing complementary information. The new BCF's have revealed some differences compared to single metal exposures when eleutheroembryos were exposed to the metal mixture, especially for iAs(III) and Cd(II). LA-ICP/MS image...
In this study we evaluated the exposure effects of mixtures of five polycyclic aromatic hydrocarb... more In this study we evaluated the exposure effects of mixtures of five polycyclic aromatic hydrocarbons (PAHs); namely, benzo[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene and chrysene on zebrafish embryos. Supplementation of the exposure media with 0.45% dimethyl sulfoxide and 50 ppm of Tween 20 could guarantee the solubilization and stabilization of the PAHs up to 24 h without affecting the embryos development. The exposure effects were tested by detecting the differential expression of a number of genes related to the aryl hydrocarbon receptor gene battery. Effects were detectable already after 6 h of exposure. After 24 h of exposure, all PAHs, except for benzo[a]anthracene, acted as potent inducers of the gene cyp1a1. Benzo[k]fluoranthene was the major inducer; the effect caused by the mixture at the lower concentration tested (1 ng ml(-1) ) was dominated by its presence. However, in the mixture at the highest concentration tested (10 ng ml(-1) ) it cause...
The concern related to the use of nanomaterials is growing nowadays, especially the risk associat... more The concern related to the use of nanomaterials is growing nowadays, especially the risk associated with their emission or exposure. One type of nanomaterials that has attracted much attention is quantum dots (QDs). QDs incorporation in consumer goods increases the probability of their entering in the environment and then into living organisms and human. In order to evaluate their potential to be bioconcentrated, zebrafish larvae have been exposed to SeCd/ZnS QDs, after performing an exhaustive characterization of these nanoparticles under the assay conditions. These data were compared with those obtained when zebrafish larvae were exposed to ionic cadmium. Finally, distribution of ionic Cd and QDs in exposed zebrafish larvae have been evaluated by Laser Ablation ICP-MS.
The European REACH legislation establishes the need to study the toxicity, persistence and bioacc... more The European REACH legislation establishes the need to study the toxicity, persistence and bioaccumulation of those chemicals with an exceeding production of 100tons and/or chemicals considered PBTs substances (Persistence, Bioaccumulation and Toxicity). Currently, the OECD technical guideline 305 is the most used protocol to determine bioconcentration factors of contaminants in aquatic environments. However, this procedure implies high cost and amount of adult fishes. Zebrafish (Danio Rerio) has been selected since this animal model has several advantageous features over other vertebrates, mainly fast embryonic development and easy growth. The analytical methodology here developed has been applied to calculate the bioconcentration factors (BCFs) of two contaminants: inorganic arsenic and tributyltin (measured as arsenic and tin). The method is based on the use of an ultrasonic probe assisted extraction for accelerating the sample treatment followed by detection using graphite furnace atomic absorption spectrometry with Zeeman correction (ZGFAAS). Results obtained for the BCFs values are in good agreement with previously reported data on freshwater aquatic organisms. In the case of arsenic, after exposing larvae to concentrations of 5 and 50μgL(-1), very low BCFs were observed (between 2.2 and 9.5); while for tributyltin, the BCFs observed were within the range 840-1280 after exposure to concentrations of 0.2 and 2.0μgL(-1), respectively. This study shows the use of zebrafish larvae together with the proposed analytical approach as a promising alternative to the OECD 305 test to evaluate the BCFs of classical and emergent contaminants.
This study examined the effects of arsenic exposure and the biotransformation capacity of develop... more This study examined the effects of arsenic exposure and the biotransformation capacity of developing Iberian green frogs (Rana perezi) with the main aim of finding valid biomarkers to monitor pollution and toxicological problems. Total arsenic concentration and arsenic ...
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