José Jesús Tejada Alvarado

Lilies are one of the most important, beautiful, and economically valuable flowers in the world. Lilium is regarded as a popular floral trade cut flower, so viable protocols are needed to provide seed production, multiplication, and preservation. In vitro protocols allow for rapid large-scale production and rejuvenation of planting material, but to be a commercially viable multiplication method, the procedure must allow for rapid production of viable, true-to-type plants quickly. The objective was to evaluate the in vitro production of microbulbs of five lily varieties (Lilium “Champion Diamond,” Lilium “Yellow Diamond,” Lilium “Batavus,” Lilium “Hyde Park,” and Lilium sp.) using different concentrations of 6-benzylaminopurine (0, 0.5, 1.0, 1.5, 1.5, and 2.0 mg L−1 BAP) and to determine the commercial quality (flowering) of the plants grown from the seed obtained. Results from the micropropagation phase show Lilium “Batavus” and Lilium “Hyde Park” varieties had better in vitro respo...

BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation. OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings. METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries. RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantl...

La aplicación de las técnicas de cultivo de tejidos en especies de Vasconcellea es una alternativa viable para la propagación de estas especies. Sin embargo, la eficiencia de estas herramientas está condicionada por factores como la contaminación de explantes, el efecto de los reguladores de crecimiento y las especies que se desea propagar. El presente estudio tuvo como objetivo evaluar protocolos de desinfección superficial, con diferentes concentraciones de NaClO y Ca(ClO)2 y tiempos de inmersión, para el establecimiento de explantes. Asimismo, evaluar la acción de las fitohormonas BAP, ANA y AG3 en diferentes concentraciones en la multiplicación in vitro de segmentos nodales de Vasconcellea chachapoyensis y Vasconcellea x heilbornii. Los experimentos se condujeron bajo un diseño completamente al azar con arreglo factorial y los datos se analizaron con la prueba de Kruskal Wallis. Los resultados muestran que al utilizar NaClO al 1,5 % por 10 min se alcanzó solo 10 % de contaminaci...

El propósito de este estudio fue evaluar el efecto del ácido indolbutírico (AIB) sobre el sobre el enraizamiento y adaptabilidad de segmentos nodales de arándano (Vaccinium corimbosum L.). Para esto, se utilizaron microtallos de arándano (Bluecrop, Legacy, Star y Biloxi) provenientes de multiplicación in vitro, los cuales fueron sometidos a diferentes dosis de AIB (100, 200, 400, 800 mg/L). El ensayo se realizó bajo un diseño completamente al azar con arreglo bifactorial, donde se evaluó el porcentaje de enraizamiento, longitud de raíz, número de raíces, número de brotes, altura de planta, número de hojas y diámetro de tallo. Los datos se analizaron mediante la prueba de Kruskal Wallis (p<0,05) y análisis de componentes principales (ACP). Los resultados mostraron que las dosis de AIB tienen efecto significativo, el rango optimo es de 100 a 200 mg/L AIB. Por otro lado, se evidencia que la variedad Biloxi presento mejores parámetros morfológicos. Asimismo, el ACP muestra que la var...

In strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rooting), and ex vitro acclimatization of strawberry. The results showed that explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves. In vitro growth was favored by the use of 1 mg L−1 zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%). In the acclimatization phase, plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables. In fact, the results of this study could be us...

The objective of this study was to evaluate the effect of indole-3-butyric acid (IBA) on the rooting of five wild accessions of blueberry (HCHA-262, HCHA-271, HCHA-283, HCHA-286, and HCHA-290) obtained from natural populations in the province of Chachapoyas (northern Peru). Buds were collected from the middle third of the plant and treated with IBA at concentrations of 0, 1,000, 2,000, and 3,000 mg L −1 . The results indicated that of the IBA doses, the 2,000-mg L −1 dose achieved the best effects on the rooting percentage and the lengths and numbers of roots and shoots of each accession studied. Accessions HCHA-262 and HCHA-283 showed greater regeneration of new seedlings than the other accessions after 45 days. The rhizogenic capacity of the cuttings varied because of the influences of both the IBA concentration and the genotype of the mother plant.