The Journal of World's Poultry Research (2322-455X) is an international, English language, peer reviewed open access journal aims to publish the high quality material from poultry scientists' works to improve domesticated birds production, food quality and safety. All accepted articles are published Quarterly in full text on the Internet. JWPR publishes the results of original scientific researches, reviews, case reports and short communications, in all fields of poultry science. Supervisors: Dr. Daryoush Babazadeh Phone: +989147697527 (Iran) +90 538 770 8824 (Turkey)
The individual egg production capacity of laying hens plays an outstanding role in achieving tota... more The individual egg production capacity of laying hens plays an outstanding role in achieving total production within a flock, which is affected by several internal and external factors. This study aimed to evaluate the effect of body condition, anatomical measurement, and age at the first laying (AFL) on the cumulative number of individual egg production (CNIEP) and laying pattern. Therefore, 172 Isa Brown laying chickens were investigated. Data on body condition and anatomical measurement were collected at the age of 16 weeks, while data on production was collected during 21 weeks of production (18-39 weeks). The obtained data were analyzed by Partial Least Square- Structural Equation Modeling (PLS-SEM) using smartPLS. The findings revealed that CNIEP could be predicted by body condition, anatomical measurement, and AFL. Compared to body condition and anatomical measurements, AFL was the most prominent factor in CNIEP. Body condition, anatomical measurement, AFL, and CNIEP had weak effects on the Isa Brown laying chickens’ patterns.
Local chickens are the most commonly raised poultry breed in rural areas of Togo, where they help... more Local chickens are the most commonly raised poultry breed in rural areas of Togo, where they help in alleviating poverty and food insecurity in households. The current study aimed to ensure the sustainable management of this genetic resource by evaluating the genetic diversity, phylogenetic relationships, and population structure of local chicken populations from five agroecological zones (Dry Savannah, Atakora, Forest, Wet Savannah, and Littoral) in Togo. Genotyping was carried out using 15 microsatellite markers on 30 unrelated individuals per agroecological zone. Genetic diversity was assessed by estimating the number of alleles per locus, observed heterozygosity, unbiased expected heterozygosity, and the polymorphic information content (PIC). The genetic structure of the populations was analyzed using a Bayesian-based approach. The results revealed a high genetic diversity but weak population structuring among local chickens. Moreover, 98 alleles were detected in all population groups, varying from 3 to 12 per locus, with an average of 6.53 ± 2.67 alleles per locus. The PIC values varied from 0.436 to 0.690, with an average of 0.550 ± 0.087. The mean number of alleles per population across all markers ranged from 4.4 ± 1.4 (Dry Savannah) to 5.4 ± 2.0 (Forest). The unbiased expected heterozygosity was high and varied from 0.58 ± 0.07 (Atakora) to 0.65 ± 0.11 (Forest), while that observed varied between 0.46 ± 0.09 (Dry Savannah) and 0.57 ± 0.14 (Forest). All populations deviated significantly from the Hardy-Weinberg equilibrium. Across populations, F IT , F IS, and F ST fixation indices were 0.150, 0.132, and 0.021, respectively. The genetic distances were low and varied from 0.022 (between Atakora and Dry Savannah) to 0.045 (between Atakora and Forest). These results could be used in potential genetic improvement programs or the preservation of local chickens in Togo.
Eruca sativa (ES) seeds are annual herbs belonging to the Brassicaceae family, widely grown in Me... more Eruca sativa (ES) seeds are annual herbs belonging to the Brassicaceae family, widely grown in Mediterranean countries, such as Egypt, Italy, and Greece. The ES is rich in macronutrient components and phytochemical content, exhibiting potent antioxidant properties and functional properties for vital processes such as digestion and absorption of nutrients. Therefore, this research was conducted to evaluate the effects of dietary ES supplementation on laying performance, some blood parameters, and egg yolk cholesterol. A total of 300 Silver Sabahia strain hens, aged 26 weeks, were randomly distributed among four groups of five replicates, each replicate consisting of 15 hens. Chickens in group 1 served as a control and were fed the basal diet. Those in groups 2, 3, and 4 were fed basal diet supplemented with 1, 2, and 3% ESs, respectively. Productive performance traits, egg quality traits, hematological parameters, blood parameters, and yolk cholesterol profiles were performed throughout the study. The study lasted for 13 weeks (until week 39 of chickens' age). Results indicated that 3% ES supplementation had higher results on egg mass (35.68%), egg production (21.13%), and improved feed conversion ratio by 30.37%, compared to all groups. Furthermore, ESs supplementation positively affects the shell thickness and yolk color score compared to the control. Compared to the control, the highest significant blood hemoglobin and lymphocytes were recorded in the groups supplemented with 2% and 3% of ESs. The ES inclusion at a higher level (3%) in the diet of laying hens led to significantly enhanced serum high-density lipoprotein and total antioxidant capacity, while reducing cholesterol, low-density lipoprotein, and malondialdehyde levels, compared to the control diet. Serum calcium, tri-iodothyronine, and alkaline phosphatase levels increased significantly in response to 3% ES treatment, while liver enzymes decreased significantly compared to the control diet. Notably, the addition of 2% and 3% ESs to the hens' ration resulted in reduced egg cholesterol content, which is desirable for consumers seeking healthier dietary choices. Finally, adding 3% ESs to hens' diet improves productive performance, egg quality traits, hematological parameters, blood parameters, and yolk cholesterol profile.
The partnership system is standard broiler cooperation in Indonesia. This system influenced agrib... more The partnership system is standard broiler cooperation in Indonesia. This system influenced agribusiness performance. Hence, the current study aimed to analyze the broiler agribusiness system in Kediri Regency, Indonesia, addressing three main areas, including partnership patterns, production performance, and financial performance. In this study, data was gathered from participants using a cross-sectional survey approach, capturing information from individuals at a specific moment in time. The research was performed from July to September 2022, utilizing both primary and secondary data. Primary data was obtained through direct observations and interviews with relevant stakeholders, while secondary data was sourced from various databases, such as the Indonesian Statistical Bureau and the Agriculture Ministry of Indonesia. Both types of data were subjected to quantitative descriptive analysis. The results indicated that the broiler partnership pattern consisted of three subsystems, including the chicken production facility providers (day-old chicks, feed, and medicines), the farming unit responsible for production process management (housing, feeding, drinking, and biosecurity), and the marketing subsystem focusing on chicken prices). The farmers in the farming unit showed effective production performance with a feed conversion ratio of 1.69, an index performance of 307, and an average body weight of 2.03 kg/head. Moreover, the farmers demonstrated a profitable financial performance with the revenue-cost ratio exceeding 1, reaching 1.07.
The climate significantly impacts the temperature in different parts of the world. A moderate env... more The climate significantly impacts the temperature in different parts of the world. A moderate environment makes it simple to construct a chicken farm. Nevertheless, raising the birds in tropical places where typical temperatures can exceed 40-45°C is difficult because they can only survive at temperatures between 30°C and 35°C. As a result, the current study aimed to design a chicken house with a ventilation and cooling system to prevent excessive heat. The effectiveness of ventilation systems in maintaining liveable and constant conditions at the chicken house was assessed using computational fluid dynamics modeling to mimic internal and external airflows. In this study, a water evaporator-based cooling system and an exhaust fan-based ventilation system were built within a poultry house. ANSYS CFD was utilized to create the design and examine the flow of the model. The findings of each model were generated individually, and these results were compared to those of the other models to determine which model could decrease the temperature within the chicken coop. The proposed model's maximum temperature was around 30-32°C. A poultry house can be constructed using this idea to maintain chickens at a suitable temperature range of 30-32°C.
Tannins are secondary metabolites and active compounds widely present in plants. Tannins have sev... more Tannins are secondary metabolites and active compounds widely present in plants. Tannins have several properties, such as astringent, antiparasitic, anti-diarrheal, anti-bacterial, and antioxidant. Hence, plants containing tannins are a major study subject for a natural alternative to in-feed antibiotics or antioxidants. The functions of tannin extracted from chestnut wood, namely Saviotan Feed (SF) in poultry, especially in quails, have not yet been fully understood. The current study aimed to examine the effect of SF supplementation on some plasma metabolites, including glucose (GLU), triglyceride (TG), total cholesterol (TCHO) concentration, and yolk cholesterol in quails (Coturnix japonica). A total of 100 unsexed quails were divided into 4 groups, with 25 quails in each group. These quails were then placed into 20 experimental pens, with 5 quails per pen. Each treatment was replicated 5 times, and the quails were fed a commercial rations diet supplemented with different SF doses of 0% (control), 0.1%, 0.2%, and 0.3%. Quails were provided with SF supplementation from 14 to 56 days of age. A sample of 40 plasma and eggs were randomly collected and analyzed for GLU, TG, and TCHO. The results indicated no significant effects of SF on plasma GLU and TG concentration, but a significant effect was found regarding TCHO. Moreover, supplementation of SF from 0.1 to 0.3% significantly decreased TCHO concentration in the yolk. In conclusion, it has been determined that supplementation of SF may play a significant role in decreasing TCHO in yolk eggs in quails.
In response to the 2006 EU ban on the use of antibiotics as growth promoters, researchers have so... more In response to the 2006 EU ban on the use of antibiotics as growth promoters, researchers have sought alternatives, leading to a focus on the beneficial effects of probiotics on chickens. The aim of this study was to evaluate the effect of the probiotic mixture containing Lactobacillus (L.) farciminis CNCM-I-3699 and Lactobacillus rhamnosus CNCM-I-3698 on the growth, production indicators, and edible organs of broiler chickens. Three trials were conducted, each consisting of 260 newly hatched Ross 308 broiler chicks (males and females) from a commercial hatchery, randomly allocated into control (n = 130) and probioticsupplemented groups (n = 130). The dietary treatments were basal diet for the control group and basal diet + the mixture of L. farciminis CNCM-I-3699 (2.10 10 GU/g) and L. rhamnosus CNCM-I-3698 (2.10 10 GU/g) at a rate of 4g/10kg of diet for the probiotic supplemented group. Broilers were raised until day 35 of age, and their body weight and feed intake were recorded on days 1, 7, 14, 21, 28, and 35. All broiler chickens were weighed on the first day. The investigated parameters included average weight gain, feed conversion ratio, cumulative feed intake, and the European Broiler Index. Daily mortality was recorded. The average organ's relative weight was calculated for each group on days 1, 7, 14, 21, 28, and 35. Although both groups yielded positive results regarding growth and production indicators, no significant differences were observed between the two groups, suggesting that probiotics may not provide expected outcomes when appropriate conditions and agerelated requirements are met. The probiotic-supplemented group exhibited significantly accelerated growth in the heart and liver. However, relative organ weights did not differ significantly between the groups.
Metal nanoparticles (NPs) are introduced into various fields of science, particularly poultry far... more Metal nanoparticles (NPs) are introduced into various fields of science, particularly poultry farming. Supplementation of metal salts in nanoform can increase the profitability of poultry farming by enhancing meat and egg production. Although their toxic parameters pose limitations on their use, many studies have evaluated the effects of using metal NPs in modern poultry farming on health, productivity, metabolism, and especially antioxidant properties. In addition, the peculiarities of their toxicokinetic and recommended doses that meet safety criteria in practical activities are highlighted. Zinc oxide NPs are one of the most studied compounds in the poultry industry. Their pronounced antioxidant properties, positive effect on productivity and homeostasis of poultry, egg quality, and immune status have been experimentally confirmed. Copper oxide NPs have similar properties but are limited in usage due to their toxicokinetics. Silver and gold NPs emerge as potential alternatives to antibiotics and could solve the resistance problem of microorganisms to antibiotics. Other important NPs used in poultry are Iron and Calcium. In their nanoform, these NPs exhibit high bioavailability, which allows for efficient absorption and utilization by poultry. The methods used to synthesize these nanoparticles make it economically viable to incorporate them into poultry diets, reducing overall expenses compared to similar macroergic compounds. Manganese and chromium NPs positively affect sperm survival in turkeys during refrigerated storage and contribute to increasing the resistance of the broiler chickens' body to heat stress and normalizing the metabolism of sex hormones. In conclusion, the application of metal nanoparticles to poultry is a promising research direction, aiming at the development of feed additives, antibiotics, and growth stimulants due to their antioxidant, bactericidal, and immunomodulatory effects.
Native chickens in Ethiopia are characterized in a fragmented manner for their performance charac... more Native chickens in Ethiopia are characterized in a fragmented manner for their performance characteristics and genotypes. This review aimed to explore the production and reproduction performance characteristics as well as the morphometric and morphological diversity of Ethiopian native chickens. The investigation was performed on four production performance characteristics, including average egg per clutch, average clutch/hen/year, average egg set/hen, and average egg/hen/year, as well as six reproductive performance characteristics, including age at first laying, age of male chickens at first bred, age at which female chickens are first bred, the reproductive life span of males and females, and fertility percentage in various parts of Ethiopia. Some economically practical morphometric characteristics of native chickens, such as shank length, chest circumference, comb length, body weight, body length, keel length, wattle length, neck length, back length, and morphological diversity, were also summarized. Regarding performance characteristics, there were some variations in eggs' average production performance per clutch (13.56-15.4 eggs) and clutch/hen/year (3.0-4.29) in Ethiopia. The average reproduction performance characteristics of Ethiopian native chickens for age at first laying (6.90-7.13 months), age of male chickens at first bred (5.87-6.15 months), female at first bred (5.20-5.93 months), the reproductive life span of males (3.79 years) and hens (3.56 years), and chicks hatched from set eggs revealed variation across Ethiopia. In various locations of Ethiopia, the average trait values reported for Ethiopian native chickens under the farmer's management differed in terms of morphometric and morphological features. The variation observed in performance characteristics, as well as morphometrics and morphological characteristics for Ethiopian native chicken ecotype population, can help the native breed classification, unique trait conservation, and breed improvement intervention programs.
The development of broiler chicken farms in Indonesia has taken two forms, namely non-contract an... more The development of broiler chicken farms in Indonesia has taken two forms, namely non-contract and contract farming. This study aimed to compare the technical efficiency levels of production in these two types of farming in Banten Province, Indonesia. Data were collected randomly from 180 broiler chicken farmers, consisting of 103 non-contract and 77 contract farmers. The study used the stochastic frontier production function to meet its objectives. The results showed that non-contract broiler chicken farmers were less efficient in their production than those under contract. The mean technical efficiency of the production factor for non-contract broiler chicken farmers was 0.689, ranging from 0.339 to 0.996. On the contrary, broiler chicken farmers under contract had a higher mean efficiency value of 0.893, ranging from 0.638 to 0.988. Moreover, the type of input supplier had a significant positive effect on technical inefficiency in non-contract farms. Non-contract farmers who purchased their production needs from a poultry shop showed higher technical efficiency compared to those who used distributors. This research sheds light on the efficiency of broiler chicken farms, both non-contract and contract, enabling all stakeholders, including the government, to devise appropriate policies for the development of broiler chicken farming. The study provided valuable insights into the technical efficiency levels of broiler chicken farming in Indonesia, which can help farmers identify areas that need improvement and develop strategies to increase productivity and profitability.
Heat stress remains a major challenge affecting poultry production in sub-tropical and tropical e... more Heat stress remains a major challenge affecting poultry production in sub-tropical and tropical environments; hence it continues to receive attention. The present study aimed to discuss heat stress and its effects on poultry production and suggests mitigation strategies to combat the effects of increased environmental temperature on poultry performance. Poultry raised in hot climates suffers from heat stress, which reduces meat and egg production, reproductive performance, feed intake, and feed conversion efficiency leading to poor growth rates. Reduced feed intake results in a reduction in meat quality, growth, egg yield, and quality. A decrease in feed utilization efficiency is the major cause of poor growth performance in hot environments. To counteract the negative impacts of high ambient temperatures on the performance of poultry, a wide range of management practices are widely used, including nutrient manipulations (particularly protein and energy), electrolyte and vitamin supplementation, feed form (especially particle size and moisture content), choice feeding, controlled feeding, time of feeding, wet feeding, water management, and use of new breeds that thrive well in hot environments. These anagement practices help lower heat load and facilitate evaporative cooling, all of which may positively impact poultry performance and health.
The animal products, such as meat, milk, skin, blood, honey, and urine, have medicinal value for ... more The animal products, such as meat, milk, skin, blood, honey, and urine, have medicinal value for human diseases. Due to having high-quality components, poultry meat has therapeutic value. The present review aimed to describe the medicinal values of poultry meat for individuals who consume it during their life. Most poultry meat is classified as white meat, which contains lower fat and higher protein, compared with the meat of ovine, bovine, and pig. This feature of poultry meat (lower fat and higher protein) helps its consumers to have a normal physiological function of different organ systems. Moreover, it prevents many non-infectious diseases, including overweight, diabetes, and cardiovascular diseases. Selenium and low contents of carcinogenic substances (myoglobin, heme iron, and saturated fat) in poultry meat also prevent different types of cancers. Poultry meat is also recommended to avoid anemia, cardiovascular diseases, and diabetes. Dietary proteins, vitamins, and minerals in chicken meat are used for anti-aging, developing muscle and bone, improving the immune system, and increasing brain function. Traditionally, poultry is recommended as a supportive treatment for respiratory diseases, such as the common cold. Thus, consumption of poultry meat, especially chickens, up to 300g/once a week is recommended to prevent and reduce the risks of gastrointestinal cancers such as oesophageal cancer. Generally, regular consumption of poultry meat has health benefits for humans to prevent and reduce the risk of different diseases as chicken meat is a rich source of nutrition that can enhance the immunity system and tackle human disease risk factors.
An appropriate agricultural policy that integrates knowledge of endogenous poultry practices shou... more An appropriate agricultural policy that integrates knowledge of endogenous poultry practices should enhance household resilience by contributing to food and nutrition security and sustainable development in developing countries. The current cross-sectional survey aimed to characterize poultry breeding systems and identify types of traditional poultry farmers in Maradi and Zinder in southern Niger. Therefore, 600 households were investigated for the socioeconomic parameters of poultry farmers, the breeding methods, the zootechnical parameters of the local chicken, and the health parameters relating to biosecurity and animal care. The results of the descriptive analyses indicated that traditional poultry activity is mainly carried out by men (73.5%) and small farmers (74.2%). Breeding management was primarily free-range breeding (99.3%). The majority of the surveyed herders (67.8%) were illiterate. However, 41.5% of them attended traditional Islamic Koranic schools. Most farmers (80%) were small-scale livestock farmers with an average herd size of 22 ± 24.9. The poultry raised were 93.3% local breeds, with chicken domination (66%). The housing did not meet the required standards, and the feed was mainly cereals. The female chicken can potentially produce 12.64 fertile eggs per clutch and brood 3.53 times per year. The leading cause of mortality in poultry was avian diseases (93.7%) and Newcastle disease in some cases. Poultry vaccination against Newcastle disease was reported by 31.5% of respondents. Of the respondents, 20% have partially observed hygiene and biosecurity measures. About 35.5% of the participants reported the provision of veterinary care, while 44% used phytotherapy to prevent or treat poultry diseases. Based on the results of this cluster analysis, three classes of poultry farmers were distinguished, each with specific characteristics. Poultry farmers in class 1 were particularly characterized by the diversity of their main activity and their level of education, those in class 2 were mostly employed in agriculture and had little school experience, and those in class 3 were characterized by their low level of vaccination practice and their lack of therapeutic animal care. The results also indicated that 15.7%, 70.8%, and 13.5% of poultry farmers belonged to classes 1, 2, and 3, respectively.
Blood tofu, or cooked duck blood curd, is a Chinese delicacy in East Asia. Its quality and shelf-... more Blood tofu, or cooked duck blood curd, is a Chinese delicacy in East Asia. Its quality and shelf-life are low due to microorganism contamination during production. Therefore, the present study was performed to investigate the role of sodium diacetate (SD), sodium chloride (NaCl), and polyethylene glycol 400 (PEG) combinations in increasing the quality of blood tofu. A total of 45 cooked duck blood curd samples were randomly divided into 3 groups with 3 replicates per group. The first two groups were used to investigate the effect of SD, NaCl, and PEG combinations on microbiological and physical analyses for non-inoculated samples. Another group was used to determine the effect of antimicrobial combinations on Lactobacillus plantarum, Pseudomonas fluorescens, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus in inoculated samples that were inoculated with these bacteria. All groups were treated with control-sterilized water, 0.15% SD (w/v) + 1.25% NaCl (w/v), 0.30% SD (w/v) + 1.25% NaCl (w/v), 0.15% SD (w/v) + 0.15% PEG (w/v), and 0.15% SD (w/v) + 1.25% NaCl (w/v) + 0.15% PEG (w/v). The results indicated that soaking cooked duck blood curd samples in antimicrobial agent combinations could reduce mesophile and psychrophile bacteria counts in non-inoculated samples. Additionally, 0.15% SD + 1.25% NaCl + 0.15% PEG combination had a higher reduction in mesophile and psychrophile counts, compared to soaking the samples in 0.30% SD + 1.25% NaCl, 0.15% SD + 1.25% NaCl and 0.15% SD + 0.15% PEG combinations. Similarly, this combination showed a significant decrease in lactic acid bacteria, Pseudomonas, Salmonella, Escherichia coli, and Staphylococcus aureus counts in inoculated samples. Furthermore, soaking the samples in 0.15% SD + 1.25% NaCl + 0.15% PEG combination did not negatively affect the samples' physical quality. Soaking the samples in 0.15% SD + 1.25% NaCl + 0.15% PEG combination inhibited the growth of mesophile, psychrophile, and Pseudomonas in non-inoculated samples after storage for 10, 6, 10, and 8 days in a slaughter warehouse at 7℃, respectively, and extended shelf-life of samples for 16 days. Regarding physical quality changes, this treatment delayed the reduction of pH, hue, hardness, and chewiness of the samples after storage for 10, 8, 12, and 10 days, respectively. Thus, SD, NaCl, and PEG combination had a high preservative potential for cooked duck blood curd used in industrial slaughterhouses.
Adeno-associated viruses (AAVs) are defective members of the genus Dependoparvovirus. Waterfowl p... more Adeno-associated viruses (AAVs) are defective members of the genus Dependoparvovirus. Waterfowl parvoviruses, another member of the Dependoparvovirus, were found to be the closest relative of AAVs. This study was performed to identify the genetic changes that may occur to goose parvovirus (GPV) in one Muscovy duck flock that was observed for 12 weeks after the virus was isolated. Persistent watery diarrhea and wing deformity were the common signs. Cloacal swabs were collected from diseased ducks. Unexpectedly, the identified virus was an AAV. The closest strains were duck AAVs at the nucleotide level, identified in Australia and China. Meanwhile, only 52.3% of nucleotide identity was shared with the GPV strain, previously identified from this flock. Duck adenovirus (DAdV) could not be identified in the samples. This study is one of the first studies in which genetic changes of GPV were tracked. In addition, emerging duck AAV from GPV is suggested, which will be useful for future virus classification.
In recent years, a rising interest has been directed towards the use of nutraceuticals in the zoo... more In recent years, a rising interest has been directed towards the use of nutraceuticals in the zootechnical sector, including probiotics, prebiotics, and synbiotics, as a way to support production efficiency and cope with the increasing limitations to the use of antibiotics. In poultry, however, most studies on these products have been conducted on broilers, while less information is available on their benefits to other productive categories. The present field study aimed to assess the effects of a multi-species synbiotic product (PoultryStar ® sol) on the gut health and productive performance of broiler breeders. A total of 24761 day-old Ross 308 parent stock chicks were acquired from a single hatchery and placed on the same farm. Female chicks were divided into three groups and raised in different houses (A, B, and C), in which males were introduced at the age of mating and followed until 40 weeks of age. The synbiotic was provided by drinking water to the flocks in houses A and B, while house C was kept as control. Following the manufacturer's guidelines, the product was administered intermittently once every two weeks, except in the first and the twenty-first week when it was supplied for three consecutive days. Data on performance parameters, egg quality traits, bacterial enteritis scoring, intestinal morphometry, and histopathology were recorded, and the caecal content was collected at 15, 25, and 40 weeks of age to investigate the intestinal microbiota using high-throughput next-generation sequencing. Synbiotic-treated hens showed significantly higher survivability during production compared to the control group. No clear differences were observed between treated and control chickens in terms of egg production and quality, and the effect of the synbiotic on weight gain also appeared limited. From 25 weeks onwards, synbiotic-treated chickens scored better in terms of macroscopical lesions and had longer intestinal villi. Significant differences in crypt length and histopathological lesions were also found at multiple sampling points. A treatment effect on caecal bacterial composition was detected with a differential abundance of Gastranaerophilales, Lachnospiraceae, Helicobacter, Ruminococcaceae, and Clostridia, among others. Taken together, obtained results support the beneficial effects of the intermittent administration of the synbiotic product PoultryStar ® sol on the gut health of broiler breeders.
In recent years, the reintroduction of the infectious bursal disease virus (IBDV), particularly i... more In recent years, the reintroduction of the infectious bursal disease virus (IBDV), particularly its severe strains,
has imposed considerable cost on the Egyptian poultry industry. The goal of the current study was to
investigate the molecular features of IBDV in Egypt from June 2019 to April 2021. A total of 30 field samples
(bursa of Fabricius) were collected from broiler farms in which the chickens were vaccinated (Transmune
2512 s/c) at hatching. A highly variable region encompassing VP2 gene was targeted for IBDV screening
utilizing reverse transcription-polymerase chain reaction (RT-PCR). Of 30 tested samples, 16 were positive by
PCR. To isolate the virus, the bursal suspension was injected into 10-11 day embryonated chicken eggs via the
chorioallantoic membrane. Five current positive isolates from 2021 were chosen for nucleotide and amino acid
(aa) sequence analysis. Phylogenetically, three of the strains under study belonged to the very virulent
(vvIBDV) strains, with 97-98% resemblance to Giza 2008 belonging to the (Genogroup 3) IBDV strain. The
remaining two strains were identified as a vaccination strain (genotype 1) that matched the winter field 2512
vaccine strain by a similarity percentage of 98. Mutations in the antigenic locations of (P) domain loops were
discovered when the sequencing samples were compared to the existing IBD vaccines. The circulating strains
were found to be very similar to vvIBDV serotype 1 genotype 3 strains with mutations in the P domain loop
providing a potential reason for the circulation of vvIBDV viruses in Egyptian broiler farms despite the
vaccination program.
The individual egg production capacity of laying hens plays an outstanding role in achieving tota... more The individual egg production capacity of laying hens plays an outstanding role in achieving total production within a flock, which is affected by several internal and external factors. This study aimed to evaluate the effect of body condition, anatomical measurement, and age at the first laying (AFL) on the cumulative number of individual egg production (CNIEP) and laying pattern. Therefore, 172 Isa Brown laying chickens were investigated. Data on body condition and anatomical measurement were collected at the age of 16 weeks, while data on production was collected during 21 weeks of production (18-39 weeks). The obtained data were analyzed by Partial Least Square- Structural Equation Modeling (PLS-SEM) using smartPLS. The findings revealed that CNIEP could be predicted by body condition, anatomical measurement, and AFL. Compared to body condition and anatomical measurements, AFL was the most prominent factor in CNIEP. Body condition, anatomical measurement, AFL, and CNIEP had weak effects on the Isa Brown laying chickens’ patterns.
Local chickens are the most commonly raised poultry breed in rural areas of Togo, where they help... more Local chickens are the most commonly raised poultry breed in rural areas of Togo, where they help in alleviating poverty and food insecurity in households. The current study aimed to ensure the sustainable management of this genetic resource by evaluating the genetic diversity, phylogenetic relationships, and population structure of local chicken populations from five agroecological zones (Dry Savannah, Atakora, Forest, Wet Savannah, and Littoral) in Togo. Genotyping was carried out using 15 microsatellite markers on 30 unrelated individuals per agroecological zone. Genetic diversity was assessed by estimating the number of alleles per locus, observed heterozygosity, unbiased expected heterozygosity, and the polymorphic information content (PIC). The genetic structure of the populations was analyzed using a Bayesian-based approach. The results revealed a high genetic diversity but weak population structuring among local chickens. Moreover, 98 alleles were detected in all population groups, varying from 3 to 12 per locus, with an average of 6.53 ± 2.67 alleles per locus. The PIC values varied from 0.436 to 0.690, with an average of 0.550 ± 0.087. The mean number of alleles per population across all markers ranged from 4.4 ± 1.4 (Dry Savannah) to 5.4 ± 2.0 (Forest). The unbiased expected heterozygosity was high and varied from 0.58 ± 0.07 (Atakora) to 0.65 ± 0.11 (Forest), while that observed varied between 0.46 ± 0.09 (Dry Savannah) and 0.57 ± 0.14 (Forest). All populations deviated significantly from the Hardy-Weinberg equilibrium. Across populations, F IT , F IS, and F ST fixation indices were 0.150, 0.132, and 0.021, respectively. The genetic distances were low and varied from 0.022 (between Atakora and Dry Savannah) to 0.045 (between Atakora and Forest). These results could be used in potential genetic improvement programs or the preservation of local chickens in Togo.
Eruca sativa (ES) seeds are annual herbs belonging to the Brassicaceae family, widely grown in Me... more Eruca sativa (ES) seeds are annual herbs belonging to the Brassicaceae family, widely grown in Mediterranean countries, such as Egypt, Italy, and Greece. The ES is rich in macronutrient components and phytochemical content, exhibiting potent antioxidant properties and functional properties for vital processes such as digestion and absorption of nutrients. Therefore, this research was conducted to evaluate the effects of dietary ES supplementation on laying performance, some blood parameters, and egg yolk cholesterol. A total of 300 Silver Sabahia strain hens, aged 26 weeks, were randomly distributed among four groups of five replicates, each replicate consisting of 15 hens. Chickens in group 1 served as a control and were fed the basal diet. Those in groups 2, 3, and 4 were fed basal diet supplemented with 1, 2, and 3% ESs, respectively. Productive performance traits, egg quality traits, hematological parameters, blood parameters, and yolk cholesterol profiles were performed throughout the study. The study lasted for 13 weeks (until week 39 of chickens' age). Results indicated that 3% ES supplementation had higher results on egg mass (35.68%), egg production (21.13%), and improved feed conversion ratio by 30.37%, compared to all groups. Furthermore, ESs supplementation positively affects the shell thickness and yolk color score compared to the control. Compared to the control, the highest significant blood hemoglobin and lymphocytes were recorded in the groups supplemented with 2% and 3% of ESs. The ES inclusion at a higher level (3%) in the diet of laying hens led to significantly enhanced serum high-density lipoprotein and total antioxidant capacity, while reducing cholesterol, low-density lipoprotein, and malondialdehyde levels, compared to the control diet. Serum calcium, tri-iodothyronine, and alkaline phosphatase levels increased significantly in response to 3% ES treatment, while liver enzymes decreased significantly compared to the control diet. Notably, the addition of 2% and 3% ESs to the hens' ration resulted in reduced egg cholesterol content, which is desirable for consumers seeking healthier dietary choices. Finally, adding 3% ESs to hens' diet improves productive performance, egg quality traits, hematological parameters, blood parameters, and yolk cholesterol profile.
The partnership system is standard broiler cooperation in Indonesia. This system influenced agrib... more The partnership system is standard broiler cooperation in Indonesia. This system influenced agribusiness performance. Hence, the current study aimed to analyze the broiler agribusiness system in Kediri Regency, Indonesia, addressing three main areas, including partnership patterns, production performance, and financial performance. In this study, data was gathered from participants using a cross-sectional survey approach, capturing information from individuals at a specific moment in time. The research was performed from July to September 2022, utilizing both primary and secondary data. Primary data was obtained through direct observations and interviews with relevant stakeholders, while secondary data was sourced from various databases, such as the Indonesian Statistical Bureau and the Agriculture Ministry of Indonesia. Both types of data were subjected to quantitative descriptive analysis. The results indicated that the broiler partnership pattern consisted of three subsystems, including the chicken production facility providers (day-old chicks, feed, and medicines), the farming unit responsible for production process management (housing, feeding, drinking, and biosecurity), and the marketing subsystem focusing on chicken prices). The farmers in the farming unit showed effective production performance with a feed conversion ratio of 1.69, an index performance of 307, and an average body weight of 2.03 kg/head. Moreover, the farmers demonstrated a profitable financial performance with the revenue-cost ratio exceeding 1, reaching 1.07.
The climate significantly impacts the temperature in different parts of the world. A moderate env... more The climate significantly impacts the temperature in different parts of the world. A moderate environment makes it simple to construct a chicken farm. Nevertheless, raising the birds in tropical places where typical temperatures can exceed 40-45°C is difficult because they can only survive at temperatures between 30°C and 35°C. As a result, the current study aimed to design a chicken house with a ventilation and cooling system to prevent excessive heat. The effectiveness of ventilation systems in maintaining liveable and constant conditions at the chicken house was assessed using computational fluid dynamics modeling to mimic internal and external airflows. In this study, a water evaporator-based cooling system and an exhaust fan-based ventilation system were built within a poultry house. ANSYS CFD was utilized to create the design and examine the flow of the model. The findings of each model were generated individually, and these results were compared to those of the other models to determine which model could decrease the temperature within the chicken coop. The proposed model's maximum temperature was around 30-32°C. A poultry house can be constructed using this idea to maintain chickens at a suitable temperature range of 30-32°C.
Tannins are secondary metabolites and active compounds widely present in plants. Tannins have sev... more Tannins are secondary metabolites and active compounds widely present in plants. Tannins have several properties, such as astringent, antiparasitic, anti-diarrheal, anti-bacterial, and antioxidant. Hence, plants containing tannins are a major study subject for a natural alternative to in-feed antibiotics or antioxidants. The functions of tannin extracted from chestnut wood, namely Saviotan Feed (SF) in poultry, especially in quails, have not yet been fully understood. The current study aimed to examine the effect of SF supplementation on some plasma metabolites, including glucose (GLU), triglyceride (TG), total cholesterol (TCHO) concentration, and yolk cholesterol in quails (Coturnix japonica). A total of 100 unsexed quails were divided into 4 groups, with 25 quails in each group. These quails were then placed into 20 experimental pens, with 5 quails per pen. Each treatment was replicated 5 times, and the quails were fed a commercial rations diet supplemented with different SF doses of 0% (control), 0.1%, 0.2%, and 0.3%. Quails were provided with SF supplementation from 14 to 56 days of age. A sample of 40 plasma and eggs were randomly collected and analyzed for GLU, TG, and TCHO. The results indicated no significant effects of SF on plasma GLU and TG concentration, but a significant effect was found regarding TCHO. Moreover, supplementation of SF from 0.1 to 0.3% significantly decreased TCHO concentration in the yolk. In conclusion, it has been determined that supplementation of SF may play a significant role in decreasing TCHO in yolk eggs in quails.
In response to the 2006 EU ban on the use of antibiotics as growth promoters, researchers have so... more In response to the 2006 EU ban on the use of antibiotics as growth promoters, researchers have sought alternatives, leading to a focus on the beneficial effects of probiotics on chickens. The aim of this study was to evaluate the effect of the probiotic mixture containing Lactobacillus (L.) farciminis CNCM-I-3699 and Lactobacillus rhamnosus CNCM-I-3698 on the growth, production indicators, and edible organs of broiler chickens. Three trials were conducted, each consisting of 260 newly hatched Ross 308 broiler chicks (males and females) from a commercial hatchery, randomly allocated into control (n = 130) and probioticsupplemented groups (n = 130). The dietary treatments were basal diet for the control group and basal diet + the mixture of L. farciminis CNCM-I-3699 (2.10 10 GU/g) and L. rhamnosus CNCM-I-3698 (2.10 10 GU/g) at a rate of 4g/10kg of diet for the probiotic supplemented group. Broilers were raised until day 35 of age, and their body weight and feed intake were recorded on days 1, 7, 14, 21, 28, and 35. All broiler chickens were weighed on the first day. The investigated parameters included average weight gain, feed conversion ratio, cumulative feed intake, and the European Broiler Index. Daily mortality was recorded. The average organ's relative weight was calculated for each group on days 1, 7, 14, 21, 28, and 35. Although both groups yielded positive results regarding growth and production indicators, no significant differences were observed between the two groups, suggesting that probiotics may not provide expected outcomes when appropriate conditions and agerelated requirements are met. The probiotic-supplemented group exhibited significantly accelerated growth in the heart and liver. However, relative organ weights did not differ significantly between the groups.
Metal nanoparticles (NPs) are introduced into various fields of science, particularly poultry far... more Metal nanoparticles (NPs) are introduced into various fields of science, particularly poultry farming. Supplementation of metal salts in nanoform can increase the profitability of poultry farming by enhancing meat and egg production. Although their toxic parameters pose limitations on their use, many studies have evaluated the effects of using metal NPs in modern poultry farming on health, productivity, metabolism, and especially antioxidant properties. In addition, the peculiarities of their toxicokinetic and recommended doses that meet safety criteria in practical activities are highlighted. Zinc oxide NPs are one of the most studied compounds in the poultry industry. Their pronounced antioxidant properties, positive effect on productivity and homeostasis of poultry, egg quality, and immune status have been experimentally confirmed. Copper oxide NPs have similar properties but are limited in usage due to their toxicokinetics. Silver and gold NPs emerge as potential alternatives to antibiotics and could solve the resistance problem of microorganisms to antibiotics. Other important NPs used in poultry are Iron and Calcium. In their nanoform, these NPs exhibit high bioavailability, which allows for efficient absorption and utilization by poultry. The methods used to synthesize these nanoparticles make it economically viable to incorporate them into poultry diets, reducing overall expenses compared to similar macroergic compounds. Manganese and chromium NPs positively affect sperm survival in turkeys during refrigerated storage and contribute to increasing the resistance of the broiler chickens' body to heat stress and normalizing the metabolism of sex hormones. In conclusion, the application of metal nanoparticles to poultry is a promising research direction, aiming at the development of feed additives, antibiotics, and growth stimulants due to their antioxidant, bactericidal, and immunomodulatory effects.
Native chickens in Ethiopia are characterized in a fragmented manner for their performance charac... more Native chickens in Ethiopia are characterized in a fragmented manner for their performance characteristics and genotypes. This review aimed to explore the production and reproduction performance characteristics as well as the morphometric and morphological diversity of Ethiopian native chickens. The investigation was performed on four production performance characteristics, including average egg per clutch, average clutch/hen/year, average egg set/hen, and average egg/hen/year, as well as six reproductive performance characteristics, including age at first laying, age of male chickens at first bred, age at which female chickens are first bred, the reproductive life span of males and females, and fertility percentage in various parts of Ethiopia. Some economically practical morphometric characteristics of native chickens, such as shank length, chest circumference, comb length, body weight, body length, keel length, wattle length, neck length, back length, and morphological diversity, were also summarized. Regarding performance characteristics, there were some variations in eggs' average production performance per clutch (13.56-15.4 eggs) and clutch/hen/year (3.0-4.29) in Ethiopia. The average reproduction performance characteristics of Ethiopian native chickens for age at first laying (6.90-7.13 months), age of male chickens at first bred (5.87-6.15 months), female at first bred (5.20-5.93 months), the reproductive life span of males (3.79 years) and hens (3.56 years), and chicks hatched from set eggs revealed variation across Ethiopia. In various locations of Ethiopia, the average trait values reported for Ethiopian native chickens under the farmer's management differed in terms of morphometric and morphological features. The variation observed in performance characteristics, as well as morphometrics and morphological characteristics for Ethiopian native chicken ecotype population, can help the native breed classification, unique trait conservation, and breed improvement intervention programs.
The development of broiler chicken farms in Indonesia has taken two forms, namely non-contract an... more The development of broiler chicken farms in Indonesia has taken two forms, namely non-contract and contract farming. This study aimed to compare the technical efficiency levels of production in these two types of farming in Banten Province, Indonesia. Data were collected randomly from 180 broiler chicken farmers, consisting of 103 non-contract and 77 contract farmers. The study used the stochastic frontier production function to meet its objectives. The results showed that non-contract broiler chicken farmers were less efficient in their production than those under contract. The mean technical efficiency of the production factor for non-contract broiler chicken farmers was 0.689, ranging from 0.339 to 0.996. On the contrary, broiler chicken farmers under contract had a higher mean efficiency value of 0.893, ranging from 0.638 to 0.988. Moreover, the type of input supplier had a significant positive effect on technical inefficiency in non-contract farms. Non-contract farmers who purchased their production needs from a poultry shop showed higher technical efficiency compared to those who used distributors. This research sheds light on the efficiency of broiler chicken farms, both non-contract and contract, enabling all stakeholders, including the government, to devise appropriate policies for the development of broiler chicken farming. The study provided valuable insights into the technical efficiency levels of broiler chicken farming in Indonesia, which can help farmers identify areas that need improvement and develop strategies to increase productivity and profitability.
Heat stress remains a major challenge affecting poultry production in sub-tropical and tropical e... more Heat stress remains a major challenge affecting poultry production in sub-tropical and tropical environments; hence it continues to receive attention. The present study aimed to discuss heat stress and its effects on poultry production and suggests mitigation strategies to combat the effects of increased environmental temperature on poultry performance. Poultry raised in hot climates suffers from heat stress, which reduces meat and egg production, reproductive performance, feed intake, and feed conversion efficiency leading to poor growth rates. Reduced feed intake results in a reduction in meat quality, growth, egg yield, and quality. A decrease in feed utilization efficiency is the major cause of poor growth performance in hot environments. To counteract the negative impacts of high ambient temperatures on the performance of poultry, a wide range of management practices are widely used, including nutrient manipulations (particularly protein and energy), electrolyte and vitamin supplementation, feed form (especially particle size and moisture content), choice feeding, controlled feeding, time of feeding, wet feeding, water management, and use of new breeds that thrive well in hot environments. These anagement practices help lower heat load and facilitate evaporative cooling, all of which may positively impact poultry performance and health.
The animal products, such as meat, milk, skin, blood, honey, and urine, have medicinal value for ... more The animal products, such as meat, milk, skin, blood, honey, and urine, have medicinal value for human diseases. Due to having high-quality components, poultry meat has therapeutic value. The present review aimed to describe the medicinal values of poultry meat for individuals who consume it during their life. Most poultry meat is classified as white meat, which contains lower fat and higher protein, compared with the meat of ovine, bovine, and pig. This feature of poultry meat (lower fat and higher protein) helps its consumers to have a normal physiological function of different organ systems. Moreover, it prevents many non-infectious diseases, including overweight, diabetes, and cardiovascular diseases. Selenium and low contents of carcinogenic substances (myoglobin, heme iron, and saturated fat) in poultry meat also prevent different types of cancers. Poultry meat is also recommended to avoid anemia, cardiovascular diseases, and diabetes. Dietary proteins, vitamins, and minerals in chicken meat are used for anti-aging, developing muscle and bone, improving the immune system, and increasing brain function. Traditionally, poultry is recommended as a supportive treatment for respiratory diseases, such as the common cold. Thus, consumption of poultry meat, especially chickens, up to 300g/once a week is recommended to prevent and reduce the risks of gastrointestinal cancers such as oesophageal cancer. Generally, regular consumption of poultry meat has health benefits for humans to prevent and reduce the risk of different diseases as chicken meat is a rich source of nutrition that can enhance the immunity system and tackle human disease risk factors.
An appropriate agricultural policy that integrates knowledge of endogenous poultry practices shou... more An appropriate agricultural policy that integrates knowledge of endogenous poultry practices should enhance household resilience by contributing to food and nutrition security and sustainable development in developing countries. The current cross-sectional survey aimed to characterize poultry breeding systems and identify types of traditional poultry farmers in Maradi and Zinder in southern Niger. Therefore, 600 households were investigated for the socioeconomic parameters of poultry farmers, the breeding methods, the zootechnical parameters of the local chicken, and the health parameters relating to biosecurity and animal care. The results of the descriptive analyses indicated that traditional poultry activity is mainly carried out by men (73.5%) and small farmers (74.2%). Breeding management was primarily free-range breeding (99.3%). The majority of the surveyed herders (67.8%) were illiterate. However, 41.5% of them attended traditional Islamic Koranic schools. Most farmers (80%) were small-scale livestock farmers with an average herd size of 22 ± 24.9. The poultry raised were 93.3% local breeds, with chicken domination (66%). The housing did not meet the required standards, and the feed was mainly cereals. The female chicken can potentially produce 12.64 fertile eggs per clutch and brood 3.53 times per year. The leading cause of mortality in poultry was avian diseases (93.7%) and Newcastle disease in some cases. Poultry vaccination against Newcastle disease was reported by 31.5% of respondents. Of the respondents, 20% have partially observed hygiene and biosecurity measures. About 35.5% of the participants reported the provision of veterinary care, while 44% used phytotherapy to prevent or treat poultry diseases. Based on the results of this cluster analysis, three classes of poultry farmers were distinguished, each with specific characteristics. Poultry farmers in class 1 were particularly characterized by the diversity of their main activity and their level of education, those in class 2 were mostly employed in agriculture and had little school experience, and those in class 3 were characterized by their low level of vaccination practice and their lack of therapeutic animal care. The results also indicated that 15.7%, 70.8%, and 13.5% of poultry farmers belonged to classes 1, 2, and 3, respectively.
Blood tofu, or cooked duck blood curd, is a Chinese delicacy in East Asia. Its quality and shelf-... more Blood tofu, or cooked duck blood curd, is a Chinese delicacy in East Asia. Its quality and shelf-life are low due to microorganism contamination during production. Therefore, the present study was performed to investigate the role of sodium diacetate (SD), sodium chloride (NaCl), and polyethylene glycol 400 (PEG) combinations in increasing the quality of blood tofu. A total of 45 cooked duck blood curd samples were randomly divided into 3 groups with 3 replicates per group. The first two groups were used to investigate the effect of SD, NaCl, and PEG combinations on microbiological and physical analyses for non-inoculated samples. Another group was used to determine the effect of antimicrobial combinations on Lactobacillus plantarum, Pseudomonas fluorescens, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus in inoculated samples that were inoculated with these bacteria. All groups were treated with control-sterilized water, 0.15% SD (w/v) + 1.25% NaCl (w/v), 0.30% SD (w/v) + 1.25% NaCl (w/v), 0.15% SD (w/v) + 0.15% PEG (w/v), and 0.15% SD (w/v) + 1.25% NaCl (w/v) + 0.15% PEG (w/v). The results indicated that soaking cooked duck blood curd samples in antimicrobial agent combinations could reduce mesophile and psychrophile bacteria counts in non-inoculated samples. Additionally, 0.15% SD + 1.25% NaCl + 0.15% PEG combination had a higher reduction in mesophile and psychrophile counts, compared to soaking the samples in 0.30% SD + 1.25% NaCl, 0.15% SD + 1.25% NaCl and 0.15% SD + 0.15% PEG combinations. Similarly, this combination showed a significant decrease in lactic acid bacteria, Pseudomonas, Salmonella, Escherichia coli, and Staphylococcus aureus counts in inoculated samples. Furthermore, soaking the samples in 0.15% SD + 1.25% NaCl + 0.15% PEG combination did not negatively affect the samples' physical quality. Soaking the samples in 0.15% SD + 1.25% NaCl + 0.15% PEG combination inhibited the growth of mesophile, psychrophile, and Pseudomonas in non-inoculated samples after storage for 10, 6, 10, and 8 days in a slaughter warehouse at 7℃, respectively, and extended shelf-life of samples for 16 days. Regarding physical quality changes, this treatment delayed the reduction of pH, hue, hardness, and chewiness of the samples after storage for 10, 8, 12, and 10 days, respectively. Thus, SD, NaCl, and PEG combination had a high preservative potential for cooked duck blood curd used in industrial slaughterhouses.
Adeno-associated viruses (AAVs) are defective members of the genus Dependoparvovirus. Waterfowl p... more Adeno-associated viruses (AAVs) are defective members of the genus Dependoparvovirus. Waterfowl parvoviruses, another member of the Dependoparvovirus, were found to be the closest relative of AAVs. This study was performed to identify the genetic changes that may occur to goose parvovirus (GPV) in one Muscovy duck flock that was observed for 12 weeks after the virus was isolated. Persistent watery diarrhea and wing deformity were the common signs. Cloacal swabs were collected from diseased ducks. Unexpectedly, the identified virus was an AAV. The closest strains were duck AAVs at the nucleotide level, identified in Australia and China. Meanwhile, only 52.3% of nucleotide identity was shared with the GPV strain, previously identified from this flock. Duck adenovirus (DAdV) could not be identified in the samples. This study is one of the first studies in which genetic changes of GPV were tracked. In addition, emerging duck AAV from GPV is suggested, which will be useful for future virus classification.
In recent years, a rising interest has been directed towards the use of nutraceuticals in the zoo... more In recent years, a rising interest has been directed towards the use of nutraceuticals in the zootechnical sector, including probiotics, prebiotics, and synbiotics, as a way to support production efficiency and cope with the increasing limitations to the use of antibiotics. In poultry, however, most studies on these products have been conducted on broilers, while less information is available on their benefits to other productive categories. The present field study aimed to assess the effects of a multi-species synbiotic product (PoultryStar ® sol) on the gut health and productive performance of broiler breeders. A total of 24761 day-old Ross 308 parent stock chicks were acquired from a single hatchery and placed on the same farm. Female chicks were divided into three groups and raised in different houses (A, B, and C), in which males were introduced at the age of mating and followed until 40 weeks of age. The synbiotic was provided by drinking water to the flocks in houses A and B, while house C was kept as control. Following the manufacturer's guidelines, the product was administered intermittently once every two weeks, except in the first and the twenty-first week when it was supplied for three consecutive days. Data on performance parameters, egg quality traits, bacterial enteritis scoring, intestinal morphometry, and histopathology were recorded, and the caecal content was collected at 15, 25, and 40 weeks of age to investigate the intestinal microbiota using high-throughput next-generation sequencing. Synbiotic-treated hens showed significantly higher survivability during production compared to the control group. No clear differences were observed between treated and control chickens in terms of egg production and quality, and the effect of the synbiotic on weight gain also appeared limited. From 25 weeks onwards, synbiotic-treated chickens scored better in terms of macroscopical lesions and had longer intestinal villi. Significant differences in crypt length and histopathological lesions were also found at multiple sampling points. A treatment effect on caecal bacterial composition was detected with a differential abundance of Gastranaerophilales, Lachnospiraceae, Helicobacter, Ruminococcaceae, and Clostridia, among others. Taken together, obtained results support the beneficial effects of the intermittent administration of the synbiotic product PoultryStar ® sol on the gut health of broiler breeders.
In recent years, the reintroduction of the infectious bursal disease virus (IBDV), particularly i... more In recent years, the reintroduction of the infectious bursal disease virus (IBDV), particularly its severe strains,
has imposed considerable cost on the Egyptian poultry industry. The goal of the current study was to
investigate the molecular features of IBDV in Egypt from June 2019 to April 2021. A total of 30 field samples
(bursa of Fabricius) were collected from broiler farms in which the chickens were vaccinated (Transmune
2512 s/c) at hatching. A highly variable region encompassing VP2 gene was targeted for IBDV screening
utilizing reverse transcription-polymerase chain reaction (RT-PCR). Of 30 tested samples, 16 were positive by
PCR. To isolate the virus, the bursal suspension was injected into 10-11 day embryonated chicken eggs via the
chorioallantoic membrane. Five current positive isolates from 2021 were chosen for nucleotide and amino acid
(aa) sequence analysis. Phylogenetically, three of the strains under study belonged to the very virulent
(vvIBDV) strains, with 97-98% resemblance to Giza 2008 belonging to the (Genogroup 3) IBDV strain. The
remaining two strains were identified as a vaccination strain (genotype 1) that matched the winter field 2512
vaccine strain by a similarity percentage of 98. Mutations in the antigenic locations of (P) domain loops were
discovered when the sequencing samples were compared to the existing IBD vaccines. The circulating strains
were found to be very similar to vvIBDV serotype 1 genotype 3 strains with mutations in the P domain loop
providing a potential reason for the circulation of vvIBDV viruses in Egyptian broiler farms despite the
vaccination program.
Poultry farming has been recognized as one of the most vital sectors for the economy and revenue ... more Poultry farming has been recognized as one of the most vital sectors for the economy and revenue generation in many countries. For the production of high-quality freshly hatched chicks, effective cleaning and sanitation of the hatchery environment and hatching eggs were crucial components of proper management and hygiene in chicken hatcheries. The current review aimed to assess the efficient ways of mitigating the risk of disease introduction (external biosecurity) and its subsequent dissemination (internal biosecurity) within and between poultry farms and hatcheries. In addition to identifying the variety of risk categories that are applied to various biosecurity industries, this article clarified the equivalent tools, including checklists and/or questionnaires, that can be used to assess biosecurity compliance. The checklist was aimed to evaluate numerous biosecurity protocol categories, including the farm's infrastructure, employees, their education and training, access control mechanisms, cleaning and disinfection procedures, handling of litter and waste, chick control, registrations, and pest management. In conclusion, external biosecurity was critical to preventing infections from entering hatcheries and poultry farms. Questionnaires or checklists were effective instruments for gathering information on biosecurity and evaluating compliance in poultry farms.
The limits of commercial diets, their quality, and their rising costs are some of the major chall... more The limits of commercial diets, their quality, and their rising costs are some of the major challenges to broiler production in Ethiopia. The purpose of this investigation was to evaluate carcass yield characteristics and blood biochemical parameters of Cobb-500 and Hubbard chicken strains fed on farm-formulated diets (T1) and three different commercial diets (T2, T3, and T4). A total of 384 mixed-sex day-old chicks (192 per strain) were randomly assigned to four dietary treatments with four replicates, each consisting of 12 broilers. The experiment was set up as a 2 × 4 factorial design, providing each strain with four diets in a completely randomized design. After 42 days of the experiment, one male and one female of each strain from each pen (eight birds per treatment) were slaughtered for carcass yield and hematological analysis. Although diets had a significant impact on live body weight, feed conversion ratio, and feed consumption among the study treatments, they had no significant effect on the mortality rate of the broilers as a whole. There was a significant effect of strains on the weight of eviscerate, dress, thigh, drumstick, breast, neck, back, and eviscerate yield percentage, with Cobb 500 showing higher values than Hubbard broilers. The farm-formulated diet (T1) significantly increased the weight of non-edible offal compared to the commercial diets, except for the weight of crops and lungs, which were similar to those in commercial diet group T4. The Hubbard strain showed a higher least square mean for packed cell volume than the Cobb-500 strain. Sex was found to have no significant impact on the hematological parameters. The farm-formulated diet (T1) also resulted in a higher marginal return rate than that of the commercial diet (T3) in the Cobb-500 strain. These findings suggest that locally sourced farm-formulated diets could be a viable alternative to commercial diets for broiler chickens in the study area.
Successful breeding programs for Japanese quails rely on accurately estimating genetic parameters... more Successful breeding programs for Japanese quails rely on accurately estimating genetic parameters linked to economically important traits such as body weight, carcass characteristics, and meat quality. The objective of the present study was to evaluate body weight (BW) characteristics, carcass attributes, and their genetic correlations with select meat quality traits in two strains of Japanese quail (white and brown). A total of 530 quail chicks, with 265 from each strain, were included in the analysis. At six weeks of age, the quails were slaughtered, and carcass traits as well as amino acid profiles were measured. For BW traits, the heritability (h 2) estimates ranged from 0.27 at d 1 to 0.36 at d 42. The h 2 estimated for carcass traits ranged from 0.19 for liver weight, to 0.42 for carcass yield (CY). The h 2 estimated for drip loss (DL) of meat quality was 0.21, and the h 2 estimate was 0.35 for the meat's ultimate Ph (Phu). White quail quails recorded the heaviest weight of all carcass traits. Also, white quails had the highest water-holding capacity (WHC), yellowness (b*), and lightness (L*) with the lowest level of DL, cooking losses (CL), and redness (a*) in muscles compared with brown quails. A high genetic correlation of 0.32 was noted between CW carcass weight (CW) and b*. For the pHU, a negative correlation of-0.11 was exhibited with BW. In contrast, L* appeared to have a positive but smaller relationship with CW and CY. High negative correlations were noted for b* with CY-0.27. The CW showed a moderate relationship (0.19) with CL. In conclusion, the current study revealed that the white quail strain had high BW, as well as the finest carcass traits and meat quality. Therefore, white plumage Japanese quail might be preferred as a meat-producing strain.
Due to the extreme importance of the poultry industry in securing animal proteins for humans, it ... more Due to the extreme importance of the poultry industry in securing animal proteins for humans, it is necessary to expand the research related to increasing egg production without resorting to antibiotics, which pose significant drawbacks. This study explored the impact of sage plant extracts, known for their bioactive compounds, on the production indicators of laying hens. Thirty chickens were randomly assigned to three groups, including a control group and two experimental groups (T1 and T2) receiving sage plant aqueous extract at 0.1% and 0.2% in their diets, respectively. The egg production percentage, egg weight percentage, percentage of daily feed consumption, feed conversion coefficient, and blood calcium concentrations were measured. The results indicated that supplementation of sage extract in the diet of the laying hens under study increased daily egg production percentage and daily egg yield significantly in group T2 (87.63%, 59.7 eggs/day) and improved average egg weight (68.23 grams) in group T1. Moreover, there was no significant difference in daily feed consumption among the tested hens. A notable reduction was also observed in the feed conversion ratio to 2.09 in group T2.
Bacterial infections, such as those caused by Pasteurella multocida serotype A, pose significant ... more Bacterial infections, such as those caused by Pasteurella multocida serotype A, pose significant threats to poultry farming. The use of antibiotics to treat these infections can lead to antibiotic resistance. The present study aimed to identify Pasteurella multocida from 14 Hisex Brown layer chicken hen farms, with chikens aged 25-55 weeks, in West Java, Indonesia, and to evaluate their resistance to various antibiotics. Three samples from each farm were collected from dead chickens having symptoms of fowl cholera. Initially, the study involved isolating and identifying isolates from liver, heart, and lung organs via polymerase chain reaction. The colony was then tested for antibiotic resistance using the disk diffusion method. The results showed that 13 samples were Pasteurella multocida and nine were serotype A. The test results also indicated that all isolates were resistant to colistin (10 µg) and sensitive to tetracycline (30 µg), amoxicillin (25 µg), enrofloxacin (5 µg), sulfamethoxazole (25 µg), lincomycin (109 µg), and ciprofloxacin (5 µg). The study concluded that none of the Pasteurella multocida type A isolates were any longer sensitive to colistin, with some isolates still sensitive to tetracycline, amoxicillin, enrofloxacin, sulfamethoxazole, lincomycin, and ciprofloxacin, and two isolates showing multidrug resistance patterns.
Neem leaf and ginger rhizome contain numerous chemical components that are biologically active an... more Neem leaf and ginger rhizome contain numerous chemical components that are biologically active and are widely utilized in medications to treat various illnesses. The purpose of the current study was to assess the effect of aqueous neem leaf and ginger rhizome extracts on the growth performance and hematological parameters in the three breeds of chicken. A total of 360 one-day-old chicks from 3 genetic groups consisting of 120 Noiler chicks, 120 Heavy Ecotype chicks, and 120 main cross chicks were considered for this study. Each breed of chickens was randomly distributed into four groups, with three replications per group. Each replication consisted of eight females and two males, raised in a deep litter system. A 3×4 factorial arrangement was employed, involving four levels of plant extracts: a control group receiving the basal diet without any extract, a group receiving 200 ml of neem extract (NE200), a group receiving 200 ml of ginger extract (GE200), and a group receiving 100 ml of neem + 100 ml of ginger extract (NE100+GE100). The chickens were evaluated for growth parameters such as initial weight (IW), final weight (FW), average daily gain (ADG), average feed intake (AFI), feed conversion ratio (FCR) as well as some haematological parameters such as haemoglobin (Hb), packed cell volume (PCV), white blood cell (WBC), red blood cell (RBC), platelet (P), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC). Noiler chickens receiving NE100+GE100 and GE200 showed the highest final body weight and daily weight gain. The results of the haematological indices revealed that the interaction effect of genotype and plant extracts on all the treatment groups were significantly different for haemoglobin (Hb), packed cell volume (PCV), white blood cell (WBC), and platelet (P). Some haematological indices such as Hb, PCV, WBC, and platelet were better for chickens receiving GE200 and NE100+GE100 compared to NE200 and control groups. In conclusion, the interaction of GE200 and NE100+GE100 with Noiler and main cross chickens was beneficial, with no adverse effects on the physiological traits and health status of the chickens 16 weeks of age.
Chickens are sensitive to environmental challenges caused by temperature. The current study aimed... more Chickens are sensitive to environmental challenges caused by temperature. The current study aimed to determine the effects of heat manipulation during embryonic development on the physiological responses of Goliath chickens. A total of 2000 hatching eggs from 48-week-old breeders were weighed, numbered, and randomly distributed equally into 4 incubators. Each incubator received 500 eggs (4 replicates of 125 eggs each). Eggs in two of the incubators were rotated hourly at a 45° angle and maintained at 37.8°C and 60% relative humidity (T0 groups). Between embryonic days (ED) 10 and 18 of incubation, the eggs from the other two incubators were heated to 38.5°C for 6 hours per day (T1 groups). The eggs were reweighed and candled, and viable eggs were moved to the hatching baskets at ED 18 of incubation. Hatching eggs were examined individually for hatching events every three hours during the final three days of incubation. On day 21, blood samples were collected from 12 chicks per group for hormonal and biochemical analyses. The evaluated blood parameters included Triiodothyronine (T3), T4 (thyroxine), cortisol, uric acid, lactate dehydrogenase, and total protein. At hatch, chicks were weighed and their quality (survival after hatching and performance standards) was evaluated. Data were collected on embryonic development, hatching window, hatching events, biochemical parameters, and hormonal concentrations. Results indicated that hatchability, chick’s weight, Tri-iodothyronine, and corticosterone were higher in the T1 group, compared to the control group. At hatch on day 21, the pipping muscle of chicks in the treated group (T1) was significantly heavier than that of the control group, while the embryonic mortality rate was significantly higher in the T0 group. In conclusion, applying heat treatment for 6 hours at 38.5°C from ED10-ED18 of embryogenesis increased significantly the hatching rate, the pipping muscle, and the chick’s weight in this study.
The significance of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in the poultry ind... more The significance of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in the poultry industry underscores the critical importance of diagnosing avian mycoplasmosis within the field of veterinary medicine. The present study aimed to compare various diagnostic methods for detecting MG and MS in Egyptian Chicken Flocks. A total of 360 samples were collected from breeder, layer, and broiler chickens from four governorates in Egypt. Conventional isolation methods and polymerase chain reaction (PCR) were used for the direct detection of MG and MS, while serum plate agglutination test (SPA) and Enzyme-linked immunosorbent assay (ELISA) were used for detecting antibodies against MG and MS. The highest detection rate of MG was found in commercial layers, followed by breeders, and broilers. Regarding MS, the highest detection rate was found in breeders, followed by commercial layers, and broilers. By comparing the used diagnostic methods, MG and MS were determined by the SPA test (40% and 31.1% respectively), ELISA test (31.7% and 23.6%), PCR (16.7% and 11.7%), and by the conventional culture method (10.8% and 3.9%). It could be concluded that the serological methods and PCR gave better sensitivity than culture methods and can be used in the diagnosis of avian mycoplasmosis.
Litter management is important for poultry housing husbandry and affects chicken performance. The... more Litter management is important for poultry housing husbandry and affects chicken performance. The present study evaluated the effect of bedding material and gender on the productive, physiological, and immunological performance of a new hybrid chicken (WINZY Line 105) under cold stress for 56 days of age. A total of 540 one-day-old broiler chicks were divided into two groups. The groups, including 270 males (M) and 270 females (F) were further divided into two subgroups , including sawdust litter (SL) and plastic slatted floor (PSF), three replicates, and 45 chickens each. Broiler chickens were raised during the winter with an average temperature of 10 o C for 56 days. Productive, physiological, and immunological performance parameters were measured. The obtained results indicated that M reared on PSF (M x PSF) recorded the highest values in body weight (BW), body weight gain (BWG), carcass characteristics, and the best values in feed conversion compared with other interaction groups during all experimental periods. In addition, F reared on PSF had higher BW, carcass, and thigh percentages, low feed intake (FI), and best feed conversion compared to females reared on SL. The M reared on PSF had the highest hepatic enzymes except AST which was higher in F reared on PSF than other treatments. However, renal function biomarkers (Creatine, Uric acid, Urea) were higher in both M and F that were reared on SL than those reared on PSF. Moreover, there was a significant interaction detected for antibody titters against avian influenza (H5) and Newcastle disease at 21 days of age suggesting that the highest values observed for M reared on PSF (M x PSF) compared with other interaction groups during the experimental period, and it was higher in F that reared on PSF than F reared on SL at 21 days of age. It can be concluded that plastic slatted floors could be an alternative to substitute wood shavings to raise broiler chickens since it was efficient from the perspective of environmental conditions and production rates.
Phytogenic feed additives play an important role in broilers' nutrition, contributing to the impr... more Phytogenic feed additives play an important role in broilers' nutrition, contributing to the improvement of the performance and quality of meat. The study aimed to evaluate the effect of Ginger (Zingiber officinale) and Cinnamon (Cinnamon zeylanicum) on broiler chicken production, fatty acid profile, and meat quality. In the present study, 140-day-old Vencob-400 broiler chicks were divided into 7 groups, including the control group (with no additives, T0), and T1 to T6 groups receiving varying concentrations of cinnamon and ginger. Accordingly, the chickens' diet in T1 was supplemented with 1.0% cinnamon, T2 with 2.0% cinnamon, T3 with 3.0% cinnamon, T4 with 1.0% ginger, T5 with 2.0% ginger, and T6 with 3.0% ginger, all calculated based on dry matter. The carcass traits, weight of immune organs, organoleptic tests, and fatty acid profile of meat (breast and thigh) were recorded after the age of 42 days. The findings indicated that the breast and thigh had the highest organ weights in group T4 compared to other groups, however, the neck, back, drumstick, wing, and heart were not affected. The inclusion of 2% cinnamon (T2) and 1% ginger (T4) in the diet, significantly enhanced the color, texture, flavor, juiciness, and overall acceptability of the meat, compared to the diet of the control group. Adding a supplement of 2% cinnamon or 1% ginger powder to the diet of broiler chickens significantly decreased the percentage of total saturated fatty acid and increased the total unsaturated fatty acid (breast and thigh). The improvement in fatty acid composition is beneficial for the quality of the broiler meat. Based on these findings, it is recommended to supplement the diet of the broiler with either 2% cinnamon or 1% ginger powder to improve the carcass parameters and quality of the meat.
In Egypt, Newcastle disease virus (NDV) strains of genotype VII are known to be mild in domestic ... more In Egypt, Newcastle disease virus (NDV) strains of genotype VII are known to be mild in domestic waterfowl and considered reservoirs. This is the first report for the detection of NDV GVII.1.1 from ducks showing severe clinical signs with high mortalities and nervous manifestations, additionally, isolation of NDV and molecular characterization for full HN and F genes were performed. In the current study, 16 backyard mallard duck flocks showing severe nervous signs with high mortalities were investigated by real-time RT-PCR using primers specific for the Fusion gene of NDV and matrix gene for avian influenza virus (AIV). Fourteen duck flocks tested positive for AIV and only two flocks tested positive for NDV infection. NDV was isolated from the trachea and brain of the same duck from each flock then full HN and F genes were sequenced. The phylogenetic analysis of the F and HN genes indicated that these strains were clustered with NDV genotype VII 1.1. The F gene had a specific mutation that cluster them in a new branch with with A11T in the signal peptide, N30S, T324A, and 480K in the hydrophobic heptad repeat (HRc) compared to the Lasota strain. The duck strains of NDV isolated from the brain had N294K in the hydrophobic heptad repeat-b (HRb) of F protein compared to the strains isolated from the trachea of the same duck, which may have a role in crossing the blood-brain barrier. The HN protein had a specific mutation that clustered them in a new branch with mutations of A4V, R15K in the cytoplasmic region, A28T in the transmembrane domain, and S76L in the HRa. In addition, HN protein had A50T, S54R T232N, P392S, and T443V, and multiple mutations were detected in the neutralizing epitopes specific to strains in the present study (N120G, K284R, S521T) that can alter virus antigenicity. The current study indicated the continuous evolution of NDV strains from genotype VII circulating in Egypt with increasing pathogenicity in ducks. The present findings demonstrated the urgent need for the vaccination of ducks and geese with killed NDV vaccines to reduce economic losses due to virus infection and prevent transmission to chickens helping in ND control in Egypt.
The rodents play a significant role in the transmission of Salmonella between farms and regions. ... more The rodents play a significant role in the transmission of Salmonella between farms and regions. The present study aimed to compare the virulence of Salmonella enteritidis isolated from fecal samples of laying hens and murine within the same poultry house but different regions in Libya using Vivo-quantitative measurement of invasiveness (chicken intestinal loop model). A total of 540 cloacal swabs from laying hens (Hy-line brown chickens) aged 36 weeks and 200 batches of murine fecal pellets were collected from the same poultry house at Gaser Bin Gisher and Furnag regions in Libya. The samples were passed on pre-enrichment broth (Buffered Peptone Water) and enrichment broths (Rappaport Vassiliadis, Selenite broth, and tetrathionate), then the samples were cultured onto Xylose Lysine Deoxycholate agar, brilliant green agar, Salmonella Shigella agar, and Hektoen enteric agar. Single colonies were selected and stained by gram stain and tested biochemically using analytical profile index (API) 20 tests. Salmonella enteritidis was isolated from all the collected samples. The invasion of Salmonella enteritidis isolated from laying hens and murine feces was significantly higher in the anterior inoculation position compared to the posterior position of jejunum in both regions. The account of Salmonella enteritidis isolated from laying feces of hens and murine at Gaser Bengasher region was significantly higher than that isolated from the AlFurnge region. In the present study, the rodents act only as mechanical transmitters without affecting Salmonella invasiveness capacity. Furthermore, the invasion of Salmonella enteritidis depends on the inoculation position in the jejunum. Moreover, the invasiveness variation of Salmonella enteritidis isolated from the Gaser Bengasher and AlFurnge regions could be attributed to the presence of different Salmonella strains in the studied area. Salmonella enteritidis isolated from poultry and murine in the current study was sensitive to gentamicin, ciprofloxacin, and enrofloxacin and resistant to doxycycline, chloramphenicol, sulfafurazol, and ampicillin.
Coccidiosis, caused by the protozoan Eimeria, is a significant disease in poultry farms worldwide... more Coccidiosis, caused by the protozoan Eimeria, is a significant disease in poultry farms worldwide, leading to substantial economic losses. Triazines, benzene-aceto-nitrile derivatives, are widely employed in the field of veterinary healthcare to combat the hazardous impacts of protozoan parasite infestation comprising coccidiosis. The current research was designed to investigate the pharmacokinetic profile of diclazuril, a member of triazines, in Japanese quails (Coturnix japonica) and domestic pigeons (Columba livia) following single oral administration at 0.3 mg/kg body weight. 78 Quails (male: female, 1:1, 7 weeks old) and 78 pigeons (male: female, 1:1, 4 weeks old) were randomly divided into 13 groups for each species (n=6 birds/ group). Plasma samples were obtained at various time intervals (at time 0 [preceding diclazuril administration], and 0.5, 1, 4, 8, 12, 24, 48, 72, 96, 120, 144, and 168 hours after diclazuril administration) to determine its concentration utilizing high-performance liquid chromatography (HPLC). The non-compartmental approach was applied to assess the pharmacokinetic parameters via the aid of WinNonlin 8.3 software. In quails and pigeons, the peak plasma concentrations were 5.35 and 9.14 μg/mL attained at 8 hours, respectively. Additionally, the elimination half-lives (T1/2λz) were 30.74 and 26.48 hours, and the area under the plasma concentration-time curve from time zero to the last sample (AUC0-last) values were 155.67 and 343.57 μg h/mL, respectively. The mean residence time was 30.71 hours in quails and 39.68 hours in pigeons. Diclazuril exhibited favorable pharmacokinetic characteristics after oral administration at a dose of 0.3 mg/kg in quails and pigeons. However, to adjust the dosage regimen for curing coccidiosis, a future study is warranted to determine the clinical efficacy against coccidia infection. Moreover, further investigation is needed to evaluate the tissue residues and calculate the withdrawal time of diclazuril in quails and pigeons.
The Libyan coast has various types of wetlands that are passed by many migratory birds throughout... more The Libyan coast has various types of wetlands that are passed by many migratory birds throughout the year, however, studies on bacterial isolation from these birds in Lybia are scarce. The present study aimed to isolate enteropathogenic bacterial species from the seagulls that migrated to the Libyan coast as well as identification of the antibiotics that are resistant to these bacteria. A total of 50 fresh fecal samples were collected from slender-billed gulls in January 2023 at Farwa Island near the city of Zuwara, in Western Libya. Bacteria were isolated by conventional culturing method, identified using the Enterosystem 18R, and antibiotic susceptibility tests were conducted on the isolated bacteria. The results revealed the isolation of 46 bacteria, but only 32 of them were identified using biochemical tests. These identified bacteria belong to six species of Enterobacteriacae, namely Citrobacter (C.) freundii, Pantoea (P.) agglomerans, Escherichia (E.) coli, Enterobacter (En.) cloacae, Serratia liquifaciens, and Proteus mirabilis, with percentages of 53.125%, 31.25%, 6.25%, 3.125%, 3.125%, and 3.125%, respectively. All isolated bacteria were 100% sensitive to gentamicin (10 µg) and ciprofloxacin (5 µg). The highest resistance result was observed against the antibiotic cefoxitin (30 µg), with both C. freundii (5 samples) and P. agglomerans (4 samples). Resistance was observed in 5 samples of C. freundii and 4 samples of P. agglomerans out of 11 samples. Resistance to antibiotics, such as azithromycin (15 µg), ceftriaxone (30 µg), and ampicillin (10 µg), was also noted in a few isolates. The results indicated that C. freundii was the most antibiotic-resistant bacterial species isolated in this study. The highest multiple antibiotic resistance index was demonstrated by bacteria C. freundii, P. agglomerans, and En. cloacae, with a value of 0.33 for each of them. In conclusion, slender-billed gulls carry multi-drug-resistant bacteria. The study recommends the implementation of a national program to survey antibiotic-resistant bacteria, determine their prevalence, and assess the presence of antibiotic residues in animal-derived food. Furthermore, the present study advises expanding scientific studies on risk analysis, and antibiotic alternatives in migratory birds.
Quails are an important alternative to chicken production for protein sources, offering many adva... more Quails are an important alternative to chicken production for protein sources, offering many advantages over other poultry species. However, raising quail faces certain challenges, such as a shortage of specified hatcheries and the lack of local markets for quail eggs and meat, particularly in Egypt. In addition, there is less interest in quail's medication and vaccine production. A significant disease affecting the health and productivity of quails is coccidiosis, which is associated with poor feed conversion ratio, lower growth performance, heightened mortality, and high cost of vaccination and treatment. Attention to quail coccidiosis and its clinical forms, diagnosis, morphological characterization, control, and prevention is very critical for improving quail meat and egg production. This review compiles scientific data on quail coccidiosis, with a focus on literature from Egypt, for classification, data analysis, and processing.
In the egg production industry, egg weight is a critical parameter influencing economic viability... more In the egg production industry, egg weight is a critical parameter influencing economic viability. The objective of the present study was to determine the effect of cage, free-range, and deep litter breeding systems on hen egg weight using meta-analysis. Articles were searched using Google Scholar, PubMed, ScienceDirect, and Web of Science yielding 175 articles of which 22 articles were included in the present study. Methodological quality was assessed using Joanna Briggs Institute guidelines. A model was used to determine the effect of breeding systems on average hen egg weight. Meta-regression analysis was used to examine the effect of the following moderators, publication year, region, chicken age, and breed. The Cochran's Q test and I 2 statistic were performed for h heterogeneity across used studies. According to the obtained results, there was no significant difference between cage and free-range on average hen egg weight (standardized mean difference (SMD) = 0.08, I 2 = 89%, 95%CI 0.19-0.34). The free-range breeding system had heavier hen egg weight than deep litter (SMD = 0.54, I 2 = 88%, 95%CI 0.01-0.08). The findings also revealed that deep litter and free range had no significant difference in average hen egg weight (SMD =-0.05, I 2 = 87%, 95%CI-0.28-0.17). Metaregression findings showed that the origin of the used articles, the age of the chickens, and the chicken breed were observed as the main reasons for heterogeneity. This meta-analysis revealed that a free-range breeding system increased the average hen egg weight.
The production of traditional synthesized calcium D-pantothenate (D-PA) is accompanied with chemi... more The production of traditional synthesized calcium D-pantothenate (D-PA) is accompanied with chemical pollution, therefore, the eco-friendly bio-fermentation technology has received widespread attention. In order to verify the safety of a novel D-PA product produced by genetically engineered bacteria (Escherichia coli K12), the authors of the current study investigated the influence of adding D-PA to the diet on growth performance and health status of broiler chickens. A total of 192 day-old healthy Arbor Acres broiler chickens with similar weight (43.21 ± 0.12 g) were randomly divided into 4 treatments with 6 replicates and 8 broiler chickens in each replicate (male and female in half). The Escherichia coli K12 was genetically engineered for the production of D-PA. The control group was fed with the basal diet containing 20 mg/kg synthesized D-PA (CT group). The treatments were supplemented with 20 (TCaP1 group, recommended dose group), 100 (TCaP5 group, 5-fold-dose group), and 200 (TCaP10 group, 10-fold-dose group) mg/kg bio-based D-PA product, respectively. The experiment lasted for 42 days and the growth performance and health status of broiler chickens were determined. The results indicated that the addition of 5- and 10-fold doses of bio-based D-PA could increase the average daily weight gain during 22-42 days of age and decrease the feed conversion rate during 22-42 and 1-42 days of age of broilers. There were some differences in white blood cell count, intermediate cell absolute value (MID) count, absolute granulocyte count, absolute lymphocyte count, granulocyte percentage, mean corpuscular volume, red blood cell distribution width-standard deviation, mean platelet volume and serum phosphorus and total bilirubin in different groups, compared with the CT group. Histological observations of the liver, spleen, pancreas, and small intestines showed that the tissue structures of various organs of the broiler chickens fed with the bio-based D-PA were clear, and no abnormal changes such as inflammatory cell infiltration and fibrous tissue hyperplasia were observed in all groups. In summary, dietary supplementation of bio-based D-PA was safe within the 10-fold- dose (200mg/kg) to broiler chickens during 1-42 days.
Egg quality could influence the price of the table and hatching eggs. However, the effects of hen... more Egg quality could influence the price of the table and hatching eggs. However, the effects of hen's live body weight on egg characteristics are poorly understood. The present study evaluated the influence of body weight (BW) on egg characteristics, such as egg width (EWD), shell weight (SW), egg length (EL), albumen weight (AW), yolk weight (YW), shell surface index (SI), albumen ratio (AR), shell ratio (SR), and yolk ratio (YR) of White Leghorn chicken. A total of 100 White Leghorn chickens at 30 weeks of age were used in the current study of which 300 eggs were collected. Live body weight was classified into three groups namely, small (≤ 195g), medium (196-220g), and large (≥ 221g). The results revealed that the White Leghorn layer's live body weight was positively influenced by the eggshell index and egg length. Additionally, the results indicated that body weight did not affect EW, SW, EWD, SR, YW, AW, AR, and YR. In conclusion, the live body weight influences egg length and shell index in the White Leghorn chicken breed.
Khat contains many bioactive compounds that are beneficial for chickens' health. Most of the chem... more Khat contains many bioactive compounds that are beneficial for chickens' health. Most of the chemical constituents found in Khat are biologically active and are used worldwide for the treatment of many diseases. However, there are few studies on the use of Khat in poultry as a nutrition, antioxidant, and antimicrobial activity. Therefore, the present study evaluated the effects of dried Khat leave (DKL) as a natural feed additive on the White Leghorn layer's performance, serum chemistry, and hematology. A total of 180 White Leghorn chickens, aged 168 days were randomly divided into four groups consisting of 45 chickens in three replications. Thirteen layers and two cocks were assigned to each replication and reared on a deep litter system. The diets of layers in T1, T2, T3, and T4 were supplemented by DKL at proportion of 0%, 0.2%, 0.4%, and 0.6%, respectively. The layers were evaluated for feed intake, body weight gain, egg production, feed conversion ratio, and some blood parameters, such as total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, albumin, globulin, red blood cells, hemoglobin, packed cells volume, white blood cells, and total protein. Feed intake in T1 (103.5 g) was significantly higher than T4 (98.5 g) and T3 (98.9 g). Layers in T1 significantly gained higher body weight than layers in other treatments. The use of DKL in layer chickens' diet did not affect egg production, egg mass, and feed conversion ratio. An increase in the level of DKL in the layers diet significantly reduced the serum high-density lipoprotein, cholesterol, and albumin. The present study showed that chickens that received a higher proportion of dried khat leaf (0.6%) significantly recorded lower levels of low-density lipoprotein and total cholesterol in their blood.
Infectious bronchitis (IB) is a disease with significant economic impacts both on the costs of co... more Infectious bronchitis (IB) is a disease with significant economic impacts both on the costs of control strategies and on productive losses. Various vaccination protocols are applied, depending on homologous or heterologous protection against IB and finding the optimal balance between costs and benefits as a choice by a responsible veterinarian. The current case study aimed to demonstrate the efficacy of a heterologous vaccine combination against field IBV GI-23 (Variant 2) infection when vaccination quality was properly monitored. Two groups, each consisting of six flocks, were examined before and after improving the quality of IB vaccine application in the hatchery. These groups were vaccinated with H-120 and 1/96 vaccine strains for heterologous protection. The study involved field visits, necropsies, serology via ELISA, and oropharyngeal sampling for RT-PCR follow-up activities. Moreover, performance parameters including average body weight, feed conversion ratio, and 7 days plus total mortality were analyzed at the end of the production cycle at 40-45 days of age. Results indicated that the group with enhanced vaccination quality in the hatchery exhibited a significant decrease in IBV titers and an absence of IBV GI-23 field infection. Additionally, there was an improvement in performance data in terms of average body weight, FCR and total mortality. Hatchery vaccination proved to be more controllable and practical compared to traditional on-farm vaccination, ensuring better control of the vaccination process and massive coverage of the farm population.
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Papers by Journal of World's Poultry Research JWPR
production and suggests mitigation strategies to combat the effects of increased environmental temperature on poultry performance. Poultry raised in hot climates suffers from heat stress, which reduces meat and egg production, reproductive performance, feed intake, and feed conversion efficiency leading to poor growth rates. Reduced feed intake results in a reduction in meat quality, growth, egg yield, and quality. A decrease in feed utilization efficiency is the major cause of poor growth performance in hot environments. To counteract the negative impacts of high ambient temperatures on the performance of poultry, a wide range of
management practices are widely used, including nutrient manipulations (particularly protein and energy), electrolyte and vitamin supplementation, feed form (especially particle size and moisture content), choice feeding, controlled feeding, time of feeding, wet feeding, water management, and use of new breeds that thrive well in hot environments. These anagement practices help lower heat load and facilitate evaporative cooling,
all of which may positively impact poultry performance and health.
has imposed considerable cost on the Egyptian poultry industry. The goal of the current study was to
investigate the molecular features of IBDV in Egypt from June 2019 to April 2021. A total of 30 field samples
(bursa of Fabricius) were collected from broiler farms in which the chickens were vaccinated (Transmune
2512 s/c) at hatching. A highly variable region encompassing VP2 gene was targeted for IBDV screening
utilizing reverse transcription-polymerase chain reaction (RT-PCR). Of 30 tested samples, 16 were positive by
PCR. To isolate the virus, the bursal suspension was injected into 10-11 day embryonated chicken eggs via the
chorioallantoic membrane. Five current positive isolates from 2021 were chosen for nucleotide and amino acid
(aa) sequence analysis. Phylogenetically, three of the strains under study belonged to the very virulent
(vvIBDV) strains, with 97-98% resemblance to Giza 2008 belonging to the (Genogroup 3) IBDV strain. The
remaining two strains were identified as a vaccination strain (genotype 1) that matched the winter field 2512
vaccine strain by a similarity percentage of 98. Mutations in the antigenic locations of (P) domain loops were
discovered when the sequencing samples were compared to the existing IBD vaccines. The circulating strains
were found to be very similar to vvIBDV serotype 1 genotype 3 strains with mutations in the P domain loop
providing a potential reason for the circulation of vvIBDV viruses in Egyptian broiler farms despite the
vaccination program.
production and suggests mitigation strategies to combat the effects of increased environmental temperature on poultry performance. Poultry raised in hot climates suffers from heat stress, which reduces meat and egg production, reproductive performance, feed intake, and feed conversion efficiency leading to poor growth rates. Reduced feed intake results in a reduction in meat quality, growth, egg yield, and quality. A decrease in feed utilization efficiency is the major cause of poor growth performance in hot environments. To counteract the negative impacts of high ambient temperatures on the performance of poultry, a wide range of
management practices are widely used, including nutrient manipulations (particularly protein and energy), electrolyte and vitamin supplementation, feed form (especially particle size and moisture content), choice feeding, controlled feeding, time of feeding, wet feeding, water management, and use of new breeds that thrive well in hot environments. These anagement practices help lower heat load and facilitate evaporative cooling,
all of which may positively impact poultry performance and health.
has imposed considerable cost on the Egyptian poultry industry. The goal of the current study was to
investigate the molecular features of IBDV in Egypt from June 2019 to April 2021. A total of 30 field samples
(bursa of Fabricius) were collected from broiler farms in which the chickens were vaccinated (Transmune
2512 s/c) at hatching. A highly variable region encompassing VP2 gene was targeted for IBDV screening
utilizing reverse transcription-polymerase chain reaction (RT-PCR). Of 30 tested samples, 16 were positive by
PCR. To isolate the virus, the bursal suspension was injected into 10-11 day embryonated chicken eggs via the
chorioallantoic membrane. Five current positive isolates from 2021 were chosen for nucleotide and amino acid
(aa) sequence analysis. Phylogenetically, three of the strains under study belonged to the very virulent
(vvIBDV) strains, with 97-98% resemblance to Giza 2008 belonging to the (Genogroup 3) IBDV strain. The
remaining two strains were identified as a vaccination strain (genotype 1) that matched the winter field 2512
vaccine strain by a similarity percentage of 98. Mutations in the antigenic locations of (P) domain loops were
discovered when the sequencing samples were compared to the existing IBD vaccines. The circulating strains
were found to be very similar to vvIBDV serotype 1 genotype 3 strains with mutations in the P domain loop
providing a potential reason for the circulation of vvIBDV viruses in Egyptian broiler farms despite the
vaccination program.