Iranian bactrian camel (Camelus bactrianus) is an endangered livestock breed with distribution in... more Iranian bactrian camel (Camelus bactrianus) is an endangered livestock breed with distribution in northwest of Iran. Microsatellites are a powerful marker for animal genetic and cell line identification and population genetic study. In this study, after producing more than 40 Camelus bactrianus fibroblast cell lines, microsatellites loci from the genome of Iranian Camelus bactrianus cell lines were identified using polymerase chain reaction (PCR)-based isolation of microsatellite arrays (PIMA) methods for genetic resources studies. PIMA is a relatively simple method which avoids not only library construction, but also radioactivity manipulation. Four polymorphic microsatellite loci (IBRC01 to IBRC04) were identified. Polymorphism of these new loci was assessed in 40 samples of Iranian bactrian camel. Number of alleles ranged from 3 to 5, observed and expected heterozygosity varied from 0.625 to 0.71 and from 0.642 to 0.807 and Shannon index from 0.89 to 1.603, respectively. The phyl...
Cryopreservation of cell lines from endangered animals is an important approach to conserve genet... more Cryopreservation of cell lines from endangered animals is an important approach to conserve genetic resources. In this study, ear fibroblasts from 51 Iranian Sistani cattle (Bos indicus) were cultured and cryopreserved in vitro, and their biological characteristics, proliferation and chromosomal features were analyzed. The results indicated that Sistani cattle fibroblasts could be successfully obtained by explant culture. The cultured cells were typical fibroblasts, with normal morphology and population doubling time of approximately 24 ± 0.5 h. Karyotyping showed that frequency of cells with chromosome number 2n = 60 was >95%. Bacterial, fungal, yeast and mycoplasma contaminations were negative. The efficiencies of VSV-G (pMDG) and lentiviral pCSGW vectors encoding fluorescent proteins were around 60%. Multiplex PCR for identification of species confirmed that Sistani cattle cell lines had no cross-contamination. The samples identification information was prepared and is available in this research. These established cell lines are valuable genetic pools from endangered Iranian Sistani cattle breed, and would be a source of many biological studies such as genomic, postgenomic and somatic cell cloning researches.
In vitro cellular & developmental biology. Animal, Jan 30, 2016
Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small hor... more Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0.5 h for all cell lines. Multiplex polymerase chain reaction (multiplex PCR) revealed that cell lines had no cross-contamination with other species. Bacteria, fungi, and mycoplasma contamination were checked using standard methods such as PCR, direct culture, and Hoechst staining. In addition to providing a valuable source for genomic, postgenomic, and somatic cloning researches, the established cell lines would preserve Caspian horse genet...
Khaledi, K.J., Panandam, J.M., Maheran, A.A. and Siraj, S.S. 2009. Reliable and fast molecular se... more Khaledi, K.J., Panandam, J.M., Maheran, A.A. and Siraj, S.S. 2009. Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers. J. Appl. Anim. Res., 35: 83–85.To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48
To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex different... more To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females ...
Abstract: The objective of this study was to assess the association of polymorphism in leptin gen... more Abstract: The objective of this study was to assess the association of polymorphism in leptin gene with breeding value of milk traits in Iranian Holstein cattle. Traits analyzed were breeding values of Milk and Fat production. A strategy employing polymerase chain reaction was used to amplify a 422 bp from blood, semen. Digestion of polymerase chain reaction products with Sau3AI revealed two alleles: Allele A was 390, 32 fragment and allele B was 303, 88 and 32 (only 303 fragment visible on the gel). Three patterns were observed ...
Khaledi, K.J., Panandam, J.M., Siraj, S.S. and Maheran, A.A. 2009. Estimation of inbreeding coeff... more Khaledi, K.J., Panandam, J.M., Siraj, S.S. and Maheran, A.A. 2009. Estimation of inbreeding coefficient in Rusa deer (Cervus timorensis) using microsatellite loci in Malaysia. J. Appl. Anim. Res, 36: 279–281.To estimate inbreeding coefficient in a small and closed population of Rusa deer (Cervus timorensis) in Malaysia, 38 individual mares were typed from blood samples at thirty nine sets of primer
Iranian bactrian camel (Camelus bactrianus) is an endangered livestock breed with distribution in... more Iranian bactrian camel (Camelus bactrianus) is an endangered livestock breed with distribution in northwest of Iran. Microsatellites are a powerful marker for animal genetic and cell line identification and population genetic study. In this study, after producing more than 40 Camelus bactrianus fibroblast cell lines, microsatellites loci from the genome of Iranian Camelus bactrianus cell lines were identified using polymerase chain reaction (PCR)-based isolation of microsatellite arrays (PIMA) methods for genetic resources studies. PIMA is a relatively simple method which avoids not only library construction, but also radioactivity manipulation. Four polymorphic microsatellite loci (IBRC01 to IBRC04) were identified. Polymorphism of these new loci was assessed in 40 samples of Iranian bactrian camel. Number of alleles ranged from 3 to 5, observed and expected heterozygosity varied from 0.625 to 0.71 and from 0.642 to 0.807 and Shannon index from 0.89 to 1.603, respectively. The phyl...
Cryopreservation of cell lines from endangered animals is an important approach to conserve genet... more Cryopreservation of cell lines from endangered animals is an important approach to conserve genetic resources. In this study, ear fibroblasts from 51 Iranian Sistani cattle (Bos indicus) were cultured and cryopreserved in vitro, and their biological characteristics, proliferation and chromosomal features were analyzed. The results indicated that Sistani cattle fibroblasts could be successfully obtained by explant culture. The cultured cells were typical fibroblasts, with normal morphology and population doubling time of approximately 24 ± 0.5 h. Karyotyping showed that frequency of cells with chromosome number 2n = 60 was >95%. Bacterial, fungal, yeast and mycoplasma contaminations were negative. The efficiencies of VSV-G (pMDG) and lentiviral pCSGW vectors encoding fluorescent proteins were around 60%. Multiplex PCR for identification of species confirmed that Sistani cattle cell lines had no cross-contamination. The samples identification information was prepared and is available in this research. These established cell lines are valuable genetic pools from endangered Iranian Sistani cattle breed, and would be a source of many biological studies such as genomic, postgenomic and somatic cell cloning researches.
In vitro cellular & developmental biology. Animal, Jan 30, 2016
Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small hor... more Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0.5 h for all cell lines. Multiplex polymerase chain reaction (multiplex PCR) revealed that cell lines had no cross-contamination with other species. Bacteria, fungi, and mycoplasma contamination were checked using standard methods such as PCR, direct culture, and Hoechst staining. In addition to providing a valuable source for genomic, postgenomic, and somatic cloning researches, the established cell lines would preserve Caspian horse genet...
Khaledi, K.J., Panandam, J.M., Maheran, A.A. and Siraj, S.S. 2009. Reliable and fast molecular se... more Khaledi, K.J., Panandam, J.M., Maheran, A.A. and Siraj, S.S. 2009. Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers. J. Appl. Anim. Res., 35: 83–85.To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48
To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex different... more To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females ...
Abstract: The objective of this study was to assess the association of polymorphism in leptin gen... more Abstract: The objective of this study was to assess the association of polymorphism in leptin gene with breeding value of milk traits in Iranian Holstein cattle. Traits analyzed were breeding values of Milk and Fat production. A strategy employing polymerase chain reaction was used to amplify a 422 bp from blood, semen. Digestion of polymerase chain reaction products with Sau3AI revealed two alleles: Allele A was 390, 32 fragment and allele B was 303, 88 and 32 (only 303 fragment visible on the gel). Three patterns were observed ...
Khaledi, K.J., Panandam, J.M., Siraj, S.S. and Maheran, A.A. 2009. Estimation of inbreeding coeff... more Khaledi, K.J., Panandam, J.M., Siraj, S.S. and Maheran, A.A. 2009. Estimation of inbreeding coefficient in Rusa deer (Cervus timorensis) using microsatellite loci in Malaysia. J. Appl. Anim. Res, 36: 279–281.To estimate inbreeding coefficient in a small and closed population of Rusa deer (Cervus timorensis) in Malaysia, 38 individual mares were typed from blood samples at thirty nine sets of primer
Uploads
Papers by K. Khaledi